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The map label for this gene is dnaN [H]

Identifier: 110677617

GI number: 110677617

Start: 206464

End: 207582

Strand: Direct

Name: dnaN [H]

Synonym: RD1_0209

Alternate gene names: 110677617

Gene position: 206464-207582 (Clockwise)

Preceding gene: 110677616

Following gene: 110677618

Centisome position: 5.0

GC content: 59.7

Gene sequence:

>1119_bases
ATGAAAATCAGCATCGAACGCGCGGCCCTGCTCAAAGCGGTGTCTCAGGCACAGTCGGTTGTGGAACGTCGCAACACCAT
CCCGATCCTTGCCAATGTGTTGATCGAAGCTGAAGGCAGCGATGTGACCTTTCGCGCCACCGACCTTGATATCGAAGTTG
TCGACAAGGCCCCCGCGCAGGTGGAACGCGCCGGGGCGACGACTGTTTCTGCCACTACCCTGCATGAGATCGTGCGCAAA
CTGCCCGACGGCGCGCTTGTGACCCTGACCGCGGATAGTGCCATCGGCCGCCTGACGGTTGAGGCCGGTCGGTCAAACTT
TTCGCTGGCAACCCTGCCCAAGGAAGACTTCCCGGTGATGGCGACGTCCGAGTATCAGTCGAATTTTTCTGTCAAGGCAC
CCGTGCTGCGACGTCTTTTCGACAAGTCGAAATTCGCGATCTCGACCGAAGAGACGCGGTATTATCTGAATGGCGTCTAC
ATGCATGTGGCGGATGCCGACGGCGGGCACGTATTGCGCTGCGTGGCGACGGATGGGCACAGGCTTGCGCGCATTGACGC
GGACCTGCCCGAGGCCGCAAACGATATGCCCGGCGTGATCGTGCCGCGCAAAACGGTTGGAGAGCTGCGCAAACTGCTCG
ACAATGACGAGATGACAATCGCGGTTTCCGTGTCGGAAACCAAGGTGCGCTTTGCCACGCCCGATATCACCCTGACGTCC
AAGGTGATTGATGGCACCTTCCCCGATTACACGCGGGTCATTCCGCAAGGCAACACGCGCAAGCTGGAAGTGGATGCGGC
GGATTTTGCCCGTGCGGTGGACCGGGTGGCAACCGTCAGCTCTGAACGCTCGCGCGCGGTCAAGCTGCAACTGGACGAAG
ACCGGTTGGTTCTGTCCGTCAACGCACCGGACAGCGGTGCCGCCGAAGAGGAATTGGCCGTGGCCTATGGCGATGAGCGG
CTGGAAATCGGGTTCAACGCGAAATACCTGCTGGAGATCGCCAGTCAGGTGGATCGTGAAAACGCCGTCTTTCTGTTCAA
TTCATCGGGCGATCCGACTCTGATGCGTGAAGGCAATGACCTGTCTGCCGTGTATGTCGTCATGCCGATGCGCGTCTGA

Upstream 100 bases:

>100_bases
GACGCCCTGCCGTTATCCTACGACAAATCCACAAAACGCTTGTGAAGTCGGCCAAACAGGGTAATTTGCCTGTCCCGACT
GTGGATTAGGAAGTGGCACA

Downstream 100 bases:

>100_bases
TGCCTTGCTCTTGTTGCCCTTGGCCGGGATAGCCACGAACGAGCTGGGGGGCATTTCATGGCCCTGCATCTGACCGATCT
CATGGTCTCGCACTTCAGGT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKISIERAALLKAVSQAQSVVERRNTIPILANVLIEAEGSDVTFRATDLDIEVVDKAPAQVERAGATTVSATTLHEIVRK
LPDGALVTLTADSAIGRLTVEAGRSNFSLATLPKEDFPVMATSEYQSNFSVKAPVLRRLFDKSKFAISTEETRYYLNGVY
MHVADADGGHVLRCVATDGHRLARIDADLPEAANDMPGVIVPRKTVGELRKLLDNDEMTIAVSVSETKVRFATPDITLTS
KVIDGTFPDYTRVIPQGNTRKLEVDAADFARAVDRVATVSSERSRAVKLQLDEDRLVLSVNAPDSGAAEEELAVAYGDER
LEIGFNAKYLLEIASQVDRENAVFLFNSSGDPTLMREGNDLSAVYVVMPMRV

Sequences:

>Translated_372_residues
MKISIERAALLKAVSQAQSVVERRNTIPILANVLIEAEGSDVTFRATDLDIEVVDKAPAQVERAGATTVSATTLHEIVRK
LPDGALVTLTADSAIGRLTVEAGRSNFSLATLPKEDFPVMATSEYQSNFSVKAPVLRRLFDKSKFAISTEETRYYLNGVY
MHVADADGGHVLRCVATDGHRLARIDADLPEAANDMPGVIVPRKTVGELRKLLDNDEMTIAVSVSETKVRFATPDITLTS
KVIDGTFPDYTRVIPQGNTRKLEVDAADFARAVDRVATVSSERSRAVKLQLDEDRLVLSVNAPDSGAAEEELAVAYGDER
LEIGFNAKYLLEIASQVDRENAVFLFNSSGDPTLMREGNDLSAVYVVMPMRV
>Mature_372_residues
MKISIERAALLKAVSQAQSVVERRNTIPILANVLIEAEGSDVTFRATDLDIEVVDKAPAQVERAGATTVSATTLHEIVRK
LPDGALVTLTADSAIGRLTVEAGRSNFSLATLPKEDFPVMATSEYQSNFSVKAPVLRRLFDKSKFAISTEETRYYLNGVY
MHVADADGGHVLRCVATDGHRLARIDADLPEAANDMPGVIVPRKTVGELRKLLDNDEMTIAVSVSETKVRFATPDITLTS
KVIDGTFPDYTRVIPQGNTRKLEVDAADFARAVDRVATVSSERSRAVKLQLDEDRLVLSVNAPDSGAAEEELAVAYGDER
LEIGFNAKYLLEIASQVDRENAVFLFNSSGDPTLMREGNDLSAVYVVMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=377, Percent_Identity=36.604774535809, Blast_Score=242, Evalue=2e-65,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40543; Mature: 40543

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISIERAALLKAVSQAQSVVERRNTIPILANVLIEAEGSDVTFRATDLDIEVVDKAPAQ
CEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCEEEEEEECCEEEEECCCCHH
VERAGATTVSATTLHEIVRKLPDGALVTLTADSAIGRLTVEAGRSNFSLATLPKEDFPVM
HHHCCCCEEHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCEEEECCCCCCCEE
ATSEYQSNFSVKAPVLRRLFDKSKFAISTEETRYYLNGVYMHVADADGGHVLRCVATDGH
EECCCCCCCCEEHHHHHHHHCCCCEEEECCCCEEEEEEEEEEEEECCCCEEEEEEECCCC
RLARIDADLPEAANDMPGVIVPRKTVGELRKLLDNDEMTIAVSVSETKVRFATPDITLTS
EEEEECCCCCCCCCCCCCEEECHHHHHHHHHHHCCCCEEEEEEEECCEEEEECCCEEEEH
KVIDGTFPDYTRVIPQGNTRKLEVDAADFARAVDRVATVSSERSRAVKLQLDEDRLVLSV
HHHCCCCCCHHEECCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEE
NAPDSGAAEEELAVAYGDERLEIGFNAKYLLEIASQVDRENAVFLFNSSGDPTLMREGND
ECCCCCCCCCCEEEEECCCEEEECCCHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCC
LSAVYVVMPMRV
CEEEEEEEEECC
>Mature Secondary Structure
MKISIERAALLKAVSQAQSVVERRNTIPILANVLIEAEGSDVTFRATDLDIEVVDKAPAQ
CEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCEEEEEEECCEEEEECCCCHH
VERAGATTVSATTLHEIVRKLPDGALVTLTADSAIGRLTVEAGRSNFSLATLPKEDFPVM
HHHCCCCEEHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCCEEEECCCCCCCEE
ATSEYQSNFSVKAPVLRRLFDKSKFAISTEETRYYLNGVYMHVADADGGHVLRCVATDGH
EECCCCCCCCEEHHHHHHHHCCCCEEEECCCCEEEEEEEEEEEEECCCCEEEEEEECCCC
RLARIDADLPEAANDMPGVIVPRKTVGELRKLLDNDEMTIAVSVSETKVRFATPDITLTS
EEEEECCCCCCCCCCCCCEEECHHHHHHHHHHHCCCCEEEEEEEECCEEEEECCCEEEEH
KVIDGTFPDYTRVIPQGNTRKLEVDAADFARAVDRVATVSSERSRAVKLQLDEDRLVLSV
HHHCCCCCCHHEECCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEE
NAPDSGAAEEELAVAYGDERLEIGFNAKYLLEIASQVDRENAVFLFNSSGDPTLMREGND
ECCCCCCCCCCEEEEECCCEEEECCCHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCC
LSAVYVVMPMRV
CEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]