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The map label for this gene is zraS [H]

Identifier: 108812985

GI number: 108812985

Start: 3180667

End: 3182481

Strand: Reverse

Name: zraS [H]

Synonym: YPN_2825

Alternate gene names: 108812985

Gene position: 3182481-3180667 (Counterclockwise)

Preceding gene: 108812987

Following gene: 108812984

Centisome position: 70.18

GC content: 43.31

Gene sequence:

>1815_bases
GTGAAGGAATCTAAAGGAAAATCGAATTTATTCTCTGTGATCCCATACGGTTTTTTTGTTGGTTTAGTGATCATTTTGAT
CGCCGTGATTATCACTATGGCGATTAAGGACCTGAATCGTGGGCGCAACATTGAATTTCAGACACTATTAGAAAAAAGCG
CCGTATTGATTCGTTCCTTTGAATCCAGCTCGCGTACAGGCATGGGAATGAGTTGGCGTCAGCGTCAAACATTGATGGAA
GAGATGGCTTACCAGCCTGGCGTTATCTATATTGCGGTTACCGACCTATCAGGGAAAATCCTTGCCCACAGTAACCCGGC
ACGTGTCGGAACGCAGCTCTATAGCCCACAAGAGATGGCGGATTTAAACGTTCAGTTAGCTGAACAGTGGCGGATAGCGG
CTTTTCTTGATGAGGATAATAAAAGTCAGGATGCTTTTGAGGTCTATCGTTTCTTTAAGCCACTGAGACGCAATAATACC
CATCAGTCCCATATGAATATGGTGAGAAGGAGTAACGTTGCTGCGACTCAAGATAATAATGACAAATATGAAAATCAAAG
TGTCATTTTTACCGCCTTCGACACTGAAGCGTTAGATGCGATGCAAGCAAAAGATATTCGCAATACGATTATCTTTTTGT
CTATTCTCAGTATGTTGGCTCTGGCGGGTATTCTGGCCCTATTCTGGGCGCGCCGTTACCAGCTTTCCCGACGCCAGCTA
CAAGACTCTAAAGCGATTTCAACCGAAATTATCAATAATCTGCCTATTGGGTTGATTACCACCAATGAAGAGCAATTGAT
CAGTGTCGTAAACCACACGGCAGAAAAGATATTCGGTATCAGTAAAGATATTCTGACCGGCCAAAATATTCACCAAGCCC
TACCTCCAGAATGGAATCTTTTAGTTAAAAAAAGTCAACGCCATCAGCCAGTAATAGAGCATGAAATTGATTATAAATTA
AAAGAAGGCCTAATTATTCCATTGAGCATTAGCGTGGCAAATATTGTTAATCACAATGGCAGTTTCCTGGGCAATATATT
CATCTTTCGGGACATGAGGGAGGTTCGGCAGTTACAAGAAGAGATACGGCGCAAAGAAAAACTGGTGGCTATTGGCAATT
TAGCCGCAGGTGTCGCGCATGAAATAAGAAATCCGCTCTCTTCTATCAAAGGTTTTGCGAAATATTTTGAGGGGCATTCC
CCGCAAGGCAGTGAGGAGCAAGAATTAGCGAAAGTGATGATAAAAGAGGTCGATAGGCTCAATAGAGCGGTCACTGAGTT
ACTGGGGTTAGTTCGCCCATCGGATTTAAGGATCCAACTCGTCAATATTAACGAGATCATTGCTCATTCACTTCATCTTA
TTCGTCAGGATGCCGATAGCAAAAAAATCACAATTCAATTTATCAGCAATGAAAACTTGCCACGAGTGGAAATAGATCCC
GATCGTTTCACTCAAGCATTACTCAACCTTTACTTAAATGCTATTCAGGCCATGGGGAGAGCGGGGACACTTGAAATTGC
CCTGGCGCTTGTCGAAGAGTCAAAATTACGAATTAGCGTAATCGATACGGGTAAAGGCATTAGAGCAGAAGATCTGGAAA
ACATCTTTAATCCTTATTTCACGACGAAAGCTTCTGGCACTGGTTTGGGCCTTGCTATCGTGCAAAAAGTGATTGAAGAG
CATCAAGGTAGAATCACCGTTACCAGCCACCCGCAATCTGGAACCCGCTTTGACATGACAATCCCCTTGGTACAACGCGG
CCAATATCGCCCAGATGGGCAAGAACAAAAGGCGTCGAATAATGACAACACATAA

Upstream 100 bases:

>100_bases
AAAACGGGTAATTATTACCCACGGTAGCGGCGATGTGGGTAATAATTACCCGCCACCAATTCATTGATACTAACCAATAA
TAAAAATAAGGATGAGAAGG

Downstream 100 bases:

>100_bases
AGCGCATATTTTGGTCGTTGATGATGACCTGAGCCACTGCACAATTATCCAGGCTTTAATGAAAGGCTGGGGTTATCAAA
CCACGCCAGCCCACAATGGA

Product: PAS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MKESKGKSNLFSVIPYGFFVGLVIILIAVIITMAIKDLNRGRNIEFQTLLEKSAVLIRSFESSSRTGMGMSWRQRQTLME
EMAYQPGVIYIAVTDLSGKILAHSNPARVGTQLYSPQEMADLNVQLAEQWRIAAFLDEDNKSQDAFEVYRFFKPLRRNNT
HQSHMNMVRRSNVAATQDNNDKYENQSVIFTAFDTEALDAMQAKDIRNTIIFLSILSMLALAGILALFWARRYQLSRRQL
QDSKAISTEIINNLPIGLITTNEEQLISVVNHTAEKIFGISKDILTGQNIHQALPPEWNLLVKKSQRHQPVIEHEIDYKL
KEGLIIPLSISVANIVNHNGSFLGNIFIFRDMREVRQLQEEIRRKEKLVAIGNLAAGVAHEIRNPLSSIKGFAKYFEGHS
PQGSEEQELAKVMIKEVDRLNRAVTELLGLVRPSDLRIQLVNINEIIAHSLHLIRQDADSKKITIQFISNENLPRVEIDP
DRFTQALLNLYLNAIQAMGRAGTLEIALALVEESKLRISVIDTGKGIRAEDLENIFNPYFTTKASGTGLGLAIVQKVIEE
HQGRITVTSHPQSGTRFDMTIPLVQRGQYRPDGQEQKASNNDNT

Sequences:

>Translated_604_residues
MKESKGKSNLFSVIPYGFFVGLVIILIAVIITMAIKDLNRGRNIEFQTLLEKSAVLIRSFESSSRTGMGMSWRQRQTLME
EMAYQPGVIYIAVTDLSGKILAHSNPARVGTQLYSPQEMADLNVQLAEQWRIAAFLDEDNKSQDAFEVYRFFKPLRRNNT
HQSHMNMVRRSNVAATQDNNDKYENQSVIFTAFDTEALDAMQAKDIRNTIIFLSILSMLALAGILALFWARRYQLSRRQL
QDSKAISTEIINNLPIGLITTNEEQLISVVNHTAEKIFGISKDILTGQNIHQALPPEWNLLVKKSQRHQPVIEHEIDYKL
KEGLIIPLSISVANIVNHNGSFLGNIFIFRDMREVRQLQEEIRRKEKLVAIGNLAAGVAHEIRNPLSSIKGFAKYFEGHS
PQGSEEQELAKVMIKEVDRLNRAVTELLGLVRPSDLRIQLVNINEIIAHSLHLIRQDADSKKITIQFISNENLPRVEIDP
DRFTQALLNLYLNAIQAMGRAGTLEIALALVEESKLRISVIDTGKGIRAEDLENIFNPYFTTKASGTGLGLAIVQKVIEE
HQGRITVTSHPQSGTRFDMTIPLVQRGQYRPDGQEQKASNNDNT
>Mature_604_residues
MKESKGKSNLFSVIPYGFFVGLVIILIAVIITMAIKDLNRGRNIEFQTLLEKSAVLIRSFESSSRTGMGMSWRQRQTLME
EMAYQPGVIYIAVTDLSGKILAHSNPARVGTQLYSPQEMADLNVQLAEQWRIAAFLDEDNKSQDAFEVYRFFKPLRRNNT
HQSHMNMVRRSNVAATQDNNDKYENQSVIFTAFDTEALDAMQAKDIRNTIIFLSILSMLALAGILALFWARRYQLSRRQL
QDSKAISTEIINNLPIGLITTNEEQLISVVNHTAEKIFGISKDILTGQNIHQALPPEWNLLVKKSQRHQPVIEHEIDYKL
KEGLIIPLSISVANIVNHNGSFLGNIFIFRDMREVRQLQEEIRRKEKLVAIGNLAAGVAHEIRNPLSSIKGFAKYFEGHS
PQGSEEQELAKVMIKEVDRLNRAVTELLGLVRPSDLRIQLVNINEIIAHSLHLIRQDADSKKITIQFISNENLPRVEIDP
DRFTQALLNLYLNAIQAMGRAGTLEIALALVEESKLRISVIDTGKGIRAEDLENIFNPYFTTKASGTGLGLAIVQKVIEE
HQGRITVTSHPQSGTRFDMTIPLVQRGQYRPDGQEQKASNNDNT

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=243, Percent_Identity=54.320987654321, Blast_Score=272, Evalue=5e-74,
Organism=Escherichia coli, GI1788549, Length=360, Percent_Identity=32.2222222222222, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1790300, Length=244, Percent_Identity=32.3770491803279, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1788713, Length=259, Percent_Identity=29.3436293436293, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1786600, Length=340, Percent_Identity=25.8823529411765, Blast_Score=87, Evalue=5e-18,
Organism=Escherichia coli, GI1790346, Length=224, Percent_Identity=28.125, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI48994928, Length=361, Percent_Identity=24.3767313019391, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1786912, Length=218, Percent_Identity=27.0642201834862, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1786783, Length=220, Percent_Identity=24.5454545454545, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI145693157, Length=225, Percent_Identity=29.3333333333333, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1790567, Length=583, Percent_Identity=22.4699828473413, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788393, Length=222, Percent_Identity=24.7747747747748, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1789149, Length=254, Percent_Identity=26.7716535433071, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1789808, Length=222, Percent_Identity=23.4234234234234, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 68175; Mature: 68175

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKESKGKSNLFSVIPYGFFVGLVIILIAVIITMAIKDLNRGRNIEFQTLLEKSAVLIRSF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
ESSSRTGMGMSWRQRQTLMEEMAYQPGVIYIAVTDLSGKILAHSNPARVGTQLYSPQEMA
CCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCHHHHHHHCCCHHHH
DLNVQLAEQWRIAAFLDEDNKSQDAFEVYRFFKPLRRNNTHQSHMNMVRRSNVAATQDNN
HCCHHHHHCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCC
DKYENQSVIFTAFDTEALDAMQAKDIRNTIIFLSILSMLALAGILALFWARRYQLSRRQL
CCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDSKAISTEIINNLPIGLITTNEEQLISVVNHTAEKIFGISKDILTGQNIHQALPPEWNL
HHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHCCCCCCE
LVKKSQRHQPVIEHEIDYKLKEGLIIPLSISVANIVNHNGSFLGNIFIFRDMREVRQLQE
EEECCCCCCCCHHHHCCHHHHCCEEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
EIRRKEKLVAIGNLAAGVAHEIRNPLSSIKGFAKYFEGHSPQGSEEQELAKVMIKEVDRL
HHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
NRAVTELLGLVRPSDLRIQLVNINEIIAHSLHLIRQDADSKKITIQFISNENLPRVEIDP
HHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEECH
DRFTQALLNLYLNAIQAMGRAGTLEIALALVEESKLRISVIDTGKGIRAEDLENIFNPYF
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCE
TTKASGTGLGLAIVQKVIEEHQGRITVTSHPQSGTRFDMTIPLVQRGQYRPDGQEQKASN
ECCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEHHHHCCCCCCCCCHHCCCC
NDNT
CCCC
>Mature Secondary Structure
MKESKGKSNLFSVIPYGFFVGLVIILIAVIITMAIKDLNRGRNIEFQTLLEKSAVLIRSF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
ESSSRTGMGMSWRQRQTLMEEMAYQPGVIYIAVTDLSGKILAHSNPARVGTQLYSPQEMA
CCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCHHHHHHHCCCHHHH
DLNVQLAEQWRIAAFLDEDNKSQDAFEVYRFFKPLRRNNTHQSHMNMVRRSNVAATQDNN
HCCHHHHHCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCC
DKYENQSVIFTAFDTEALDAMQAKDIRNTIIFLSILSMLALAGILALFWARRYQLSRRQL
CCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDSKAISTEIINNLPIGLITTNEEQLISVVNHTAEKIFGISKDILTGQNIHQALPPEWNL
HHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHCCCCCCE
LVKKSQRHQPVIEHEIDYKLKEGLIIPLSISVANIVNHNGSFLGNIFIFRDMREVRQLQE
EEECCCCCCCCHHHHCCHHHHCCEEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
EIRRKEKLVAIGNLAAGVAHEIRNPLSSIKGFAKYFEGHSPQGSEEQELAKVMIKEVDRL
HHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
NRAVTELLGLVRPSDLRIQLVNINEIIAHSLHLIRQDADSKKITIQFISNENLPRVEIDP
HHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEECH
DRFTQALLNLYLNAIQAMGRAGTLEIALALVEESKLRISVIDTGKGIRAEDLENIFNPYF
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCE
TTKASGTGLGLAIVQKVIEEHQGRITVTSHPQSGTRFDMTIPLVQRGQYRPDGQEQKASN
ECCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEHHHHCCCCCCCCCHHCCCC
NDNT
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]