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The map label for this gene is rafR [H]

Identifier: 108812552

GI number: 108812552

Start: 2673205

End: 2673462

Strand: Reverse

Name: rafR [H]

Synonym: YPN_2391

Alternate gene names: 108812552

Gene position: 2673462-2673205 (Counterclockwise)

Preceding gene: 108812553

Following gene: 108812547

Centisome position: 58.96

GC content: 45.74

Gene sequence:

>258_bases
TTGGGCCGTTGGTGGAGATATAAGTGGATCACTTTTCATCCGTCGTTGACACTTATCGCTTATGATGGGCTACCGGATGA
TAGTTTGTTAGATATTGCCGTGACCCCTATTGTTCAAAATACGCGTACCAGTGTAGGTAAACAAATTGCATCAATGATTT
GTGATTTACTGGGGGGGAAGGATCCTAAAGAACTACAGGTCCTCTGGCAGCCAGAAATAGGAGAGGGTGAAACCGACGGG
GTGAATCGACGTGGATAA

Upstream 100 bases:

>100_bases
ACATGTCGTTCAAATCAAAGGAGAAAGCTATCGACTCAGACAGAAACGAAAGGCCGGGGTTATAGCAGAAGCTAATCCTG
AGTAAAACGGTGGATCAATA

Downstream 100 bases:

>100_bases
CTGGCTATTTGGGTCGGGTCGGGAAAGGGAAGGGAGAGGAAGGGTGCTGTTGAGCCGCTGAGACTAGACGTAGATAAGCG
AGGAGAGTAACTCAGTGATA

Product: ThuR, regulatory protein for trehalosemaltose transport

Products: NA

Alternate protein names: Raffinose operon repressor [H]

Number of amino acids: Translated: 85; Mature: 84

Protein sequence:

>85_residues
MGRWWRYKWITFHPSLTLIAYDGLPDDSLLDIAVTPIVQNTRTSVGKQIASMICDLLGGKDPKELQVLWQPEIGEGETDG
VNRRG

Sequences:

>Translated_85_residues
MGRWWRYKWITFHPSLTLIAYDGLPDDSLLDIAVTPIVQNTRTSVGKQIASMICDLLGGKDPKELQVLWQPEIGEGETDG
VNRRG
>Mature_84_residues
GRWWRYKWITFHPSLTLIAYDGLPDDSLLDIAVTPIVQNTRTSVGKQIASMICDLLGGKDPKELQVLWQPEIGEGETDGV
NRRG

Specific function: The raffinose (raf) operon is negatively controlled by the specific binding of raf repressor to raf operator (rafO) DNA. Binds raffinose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 9569; Mature: 9438

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRWWRYKWITFHPSLTLIAYDGLPDDSLLDIAVTPIVQNTRTSVGKQIASMICDLLGGK
CCCEEEEEEEEECCCEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
DPKELQVLWQPEIGEGETDGVNRRG
CCCCEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GRWWRYKWITFHPSLTLIAYDGLPDDSLLDIAVTPIVQNTRTSVGKQIASMICDLLGGK
CCEEEEEEEEECCCEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
DPKELQVLWQPEIGEGETDGVNRRG
CCCCEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2180920 [H]