Click here to switch to the map view.

The map label for this gene is istB [H]

Identifier: 108812553

GI number: 108812553

Start: 2673478

End: 2673771

Strand: Reverse

Name: istB [H]

Synonym: YPN_2392

Alternate gene names: 108812553

Gene position: 2673771-2673478 (Counterclockwise)

Preceding gene: 108812554

Following gene: 108812552

Centisome position: 58.96

GC content: 50.0

Gene sequence:

>294_bases
ATGGCCCCCCGCCTGCTCATCATTGATGAAATAGGCTATCTGCCGTTCAGTCAGGAAGAAGCAAAGCTGTTCTTCCAGGT
CATCGCTAAACGTTACGAAAAGAGCGCAATGATCCTGACATCCAATCTGCCGTTCGGGCAGTGGGATCAAACGTTCGCCG
GTGATGCAGCACTGACCTCAGCGATGCTGGACCGTATCTTACACCACTCACATGTCGTTCAAATCAAAGGAGAAAGCTAT
CGACTCAGACAGAAACGAAAGGCCGGGGTTATAGCAGAAGCTAATCCTGAGTAA

Upstream 100 bases:

>100_bases
AGGTATCAAAGTTCGCTTCACAACAGCAGCAGATCTGTTACTTCAGTAATCTACGGCACAACGTCAGGGCCGTTATAAAA
CGACGCTTCAGCGTGGAGTA

Downstream 100 bases:

>100_bases
AACGGTGGATCAATATTGGGCCGTTGGTGGAGATATAAGTGGATCACTTTTCATCCGTCGTTGACACTTATCGCTTATGA
TGGGCTACCGGATGATAGTT

Product: IS100 transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 97; Mature: 96

Protein sequence:

>97_residues
MAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLDRILHHSHVVQIKGESY
RLRQKRKAGVIAEANPE

Sequences:

>Translated_97_residues
MAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLDRILHHSHVVQIKGESY
RLRQKRKAGVIAEANPE
>Mature_96_residues
APRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLDRILHHSHVVQIKGESYR
LRQKRKAGVIAEANPE

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 11017; Mature: 10886

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTS
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHH
AMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE
HHHHHHHCCCEEEEECCCCHHHHHHHHCCEEECCCCC
>Mature Secondary Structure 
APRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTS
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHH
AMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE
HHHHHHHCCCEEEEECCCCHHHHHHHHCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]