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The map label for this gene is opuCB [H]

Identifier: 108801927

GI number: 108801927

Start: 5254460

End: 5255254

Strand: Reverse

Name: opuCB [H]

Synonym: Mmcs_4964

Alternate gene names: 108801927

Gene position: 5255254-5254460 (Counterclockwise)

Preceding gene: 108801928

Following gene: 108801926

Centisome position: 92.11

GC content: 71.19

Gene sequence:

>795_bases
GTGAACTTCCTGTCCGAGGCGCTGTCGTTCATCTTCACCGCCGCCAACTGGGCCGGCCCGGCAGGGCTGGGCGCGCGCAT
CGTCGAACACCTCGAGTACACCGTGATCGCGGTCGTGTTCTCCGCGCTGATCGCCGTCCCGCTGGGAATGATCATCGGCC
ACACCGGGAAGGGCACGTTCCTGGTCGTGACGGGGGTCAACGCATTGCGCGCGCTGCCCACCCTCGGGGTGCTGCTGCTC
GGCGTACTGCTGTGGGGGCTGGGCCTGGTCGGCCCGACCGTCGCGCTGATGTTGCTCGGCATCCCGCCGCTGCTGGCGGG
GACCTACTCCGGGATCGCCAACGTCGACCCGGCGGTGGTCGACGCGGCGCGGTCGATGGGGATGACCGAGCGCCGGGTGC
TGCTGCGGGTGGAGGTGCCGATCGCGCTGCCCCTGATTCTCGGTGGGCTGCGCACGGCGACCCTGCAGATCGTGGCCACC
GCGACGGTCGCCGCCTACGCGAGCCTCGGCGGGTTGGGCCGCTACCTCATCGACGGCATCAAGGTCCGGGAGTTCTATCT
GGCGCTCGTGGGCGCGCTGATGGTGACGGCGCTGGCGCTCATCCTCGACGGGTTGCTCGCGTTCGCGGTGTGGCTGTCGG
TGCCGGGCACCGGGCGGTGGCGCCGCGCGGCCCGGATGCCGCAGCCGCTGCTGGGCGACGAGGTGGCGCTGGAGTCACGA
AGTCGCGGCGGATCGCGGTCGACTTCGGTAGCGGGCTACGAACCGGCGAGCGCGTCGCCTACGGTAGAAGGGTGA

Upstream 100 bases:

>100_bases
TGGCGGTGGTGATCGACACCCTGATCATGCTGATCGGCAAAGCCCTGACCCCCTGGGCCAGGGCCACCCGCCCGGCCCGG
CAGAAGGCGGGGGCGGCCGC

Downstream 100 bases:

>100_bases
GTGAAGCCGACCGCTCTGACCCGAAGAACACCTGGCCCGCGGTCCTGACGTGGCGGGCGCACGACGTCGCCCGGATGGAA
TCCGTCCGGGTGCAGTTGTC

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MNFLSEALSFIFTAANWAGPAGLGARIVEHLEYTVIAVVFSALIAVPLGMIIGHTGKGTFLVVTGVNALRALPTLGVLLL
GVLLWGLGLVGPTVALMLLGIPPLLAGTYSGIANVDPAVVDAARSMGMTERRVLLRVEVPIALPLILGGLRTATLQIVAT
ATVAAYASLGGLGRYLIDGIKVREFYLALVGALMVTALALILDGLLAFAVWLSVPGTGRWRRAARMPQPLLGDEVALESR
SRGGSRSTSVAGYEPASASPTVEG

Sequences:

>Translated_264_residues
MNFLSEALSFIFTAANWAGPAGLGARIVEHLEYTVIAVVFSALIAVPLGMIIGHTGKGTFLVVTGVNALRALPTLGVLLL
GVLLWGLGLVGPTVALMLLGIPPLLAGTYSGIANVDPAVVDAARSMGMTERRVLLRVEVPIALPLILGGLRTATLQIVAT
ATVAAYASLGGLGRYLIDGIKVREFYLALVGALMVTALALILDGLLAFAVWLSVPGTGRWRRAARMPQPLLGDEVALESR
SRGGSRSTSVAGYEPASASPTVEG
>Mature_264_residues
MNFLSEALSFIFTAANWAGPAGLGARIVEHLEYTVIAVVFSALIAVPLGMIIGHTGKGTFLVVTGVNALRALPTLGVLLL
GVLLWGLGLVGPTVALMLLGIPPLLAGTYSGIANVDPAVVDAARSMGMTERRVLLRVEVPIALPLILGGLRTATLQIVAT
ATVAAYASLGGLGRYLIDGIKVREFYLALVGALMVTALALILDGLLAFAVWLSVPGTGRWRRAARMPQPLLGDEVALESR
SRGGSRSTSVAGYEPASASPTVEG

Specific function: Involved in a high affinity multicomponent binding- protein-dependent transport system for glycine betaine, carnitine and choline; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1174

COG function: function code E; ABC-type proline/glycine betaine transport systems, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=166, Percent_Identity=29.5180722891566, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788449, Length=137, Percent_Identity=34.3065693430657, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788451, Length=196, Percent_Identity=29.0816326530612, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 27321; Mature: 27321

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFLSEALSFIFTAANWAGPAGLGARIVEHLEYTVIAVVFSALIAVPLGMIIGHTGKGTF
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
LVVTGVNALRALPTLGVLLLGVLLWGLGLVGPTVALMLLGIPPLLAGTYSGIANVDPAVV
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHH
DAARSMGMTERRVLLRVEVPIALPLILGGLRTATLQIVATATVAAYASLGGLGRYLIDGI
HHHHHCCCHHHEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVREFYLALVGALMVTALALILDGLLAFAVWLSVPGTGRWRRAARMPQPLLGDEVALESR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHH
SRGGSRSTSVAGYEPASASPTVEG
CCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNFLSEALSFIFTAANWAGPAGLGARIVEHLEYTVIAVVFSALIAVPLGMIIGHTGKGTF
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
LVVTGVNALRALPTLGVLLLGVLLWGLGLVGPTVALMLLGIPPLLAGTYSGIANVDPAVV
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHH
DAARSMGMTERRVLLRVEVPIALPLILGGLRTATLQIVATATVAAYASLGGLGRYLIDGI
HHHHHCCCHHHEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVREFYLALVGALMVTALALILDGLLAFAVWLSVPGTGRWRRAARMPQPLLGDEVALESR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHH
SRGGSRSTSVAGYEPASASPTVEG
CCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10216873; 9384377 [H]