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The map label for this gene is pduE [H]

Identifier: 108801916

GI number: 108801916

Start: 5245134

End: 5245466

Strand: Reverse

Name: pduE [H]

Synonym: Mmcs_4953

Alternate gene names: 108801916

Gene position: 5245466-5245134 (Counterclockwise)

Preceding gene: 108801917

Following gene: 108801915

Centisome position: 91.94

GC content: 67.87

Gene sequence:

>333_bases
ATGACCGAGAAATTCACGGTGGCGGCCGCCGTCGACGGCAAGCTGACGCTGTCGGATCTGCGGATGGACCCGGCCACGCT
GGCCTACCAGGCGGTCGTCGCCGAGCAGGACGGCAATCCGCAACTGGCCGAGAACTTCCTGCGCGCAGCGGAATTGGCCG
TCATCGACGACGAGGCGGTGATGAAATTCTACGAGGCGCTGCGTCCGCACCGGTCGACGGCCGCCGAACTGGAGGAGTTG
CGGGTGTCGCTGGAGACCGGCGGGGCATCGCGGTGCGCCGAACTCGTCCGCCAGGCCGCCGAGGTCTACGCGAGACGAGG
TCTGTTGCGGTGA

Upstream 100 bases:

>100_bases
TCTCCGCCCGCTACCACGCCCGAGCCGTGGCGTTGGTGGCGCTGGAACGCCAGGCCTGCGAACCGGGGCAGGCACCGATC
ACCGTGGAGGCCAAACGAGT

Downstream 100 bases:

>100_bases
GCCGGGATTCGGTCGTCGTGGCCGGGTGCGATGTCGGCAACCACACCACCGAGATCGTGCTCGCCCGCGTCGCCGCCGAC
GGTGTGGTCGAGCCGTTGAC

Product: propanediol utilization dehydratase small subunit

Products: NA

Alternate protein names: Propanediol utilization protein pduE [H]

Number of amino acids: Translated: 110; Mature: 109

Protein sequence:

>110_residues
MTEKFTVAAAVDGKLTLSDLRMDPATLAYQAVVAEQDGNPQLAENFLRAAELAVIDDEAVMKFYEALRPHRSTAAELEEL
RVSLETGGASRCAELVRQAAEVYARRGLLR

Sequences:

>Translated_110_residues
MTEKFTVAAAVDGKLTLSDLRMDPATLAYQAVVAEQDGNPQLAENFLRAAELAVIDDEAVMKFYEALRPHRSTAAELEEL
RVSLETGGASRCAELVRQAAEVYARRGLLR
>Mature_109_residues
TEKFTVAAAVDGKLTLSDLRMDPATLAYQAVVAEQDGNPQLAENFLRAAELAVIDDEAVMKFYEALRPHRSTAAELEELR
VSLETGGASRCAELVRQAAEVYARRGLLR

Specific function: Part of the pduCDE complex that catalyzes the dehydration of 1,2-propanediol to propionaldehyde. Is required for S.typhimurium growth on 1,2-propanediol as the sole carbon and energy source [H]

COG id: COG4910

COG function: function code Q; Propanediol dehydratase, small subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diol/glycerol dehydratase small subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003207 [H]

Pfam domain/function: PF02287 Dehydratase_SU [H]

EC number: =4.2.1.28 [H]

Molecular weight: Translated: 12053; Mature: 11922

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKFTVAAAVDGKLTLSDLRMDPATLAYQAVVAEQDGNPQLAENFLRAAELAVIDDEAV
CCCCEEEEEECCCEEEHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHH
MKFYEALRPHRSTAAELEELRVSLETGGASRCAELVRQAAEVYARRGLLR
HHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TEKFTVAAAVDGKLTLSDLRMDPATLAYQAVVAEQDGNPQLAENFLRAAELAVIDDEAV
CCCEEEEEECCCEEEHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHH
MKFYEALRPHRSTAAELEELRVSLETGGASRCAELVRQAAEVYARRGLLR
HHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9352910; 10498708; 11677609 [H]