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The map label for this gene is yjfH [C]

Identifier: 108801430

GI number: 108801430

Start: 4748752

End: 4749564

Strand: Reverse

Name: yjfH [C]

Synonym: Mmcs_4467

Alternate gene names: 108801430

Gene position: 4749564-4748752 (Counterclockwise)

Preceding gene: 108801431

Following gene: 108801429

Centisome position: 83.25

GC content: 71.46

Gene sequence:

>813_bases
TTGACGAGCGTCATCGACGTCGACGACCCCGCCGATCCGCGCCTCGACGACTTCCGGGACCTCAACAGCATCGACCGCAG
ACCCGATCTGCCCAGCGGCAAGGGTTTGGTGATCGCCGAGGGTGTGCTGGTGGTGCAGCGCATGGTGGCCTCGCGGTTCA
CCCCGCGCGCGCTGCTCGGCACCGACCGCCGGCTCACCGAACTCGGTACCGACCTCGACGCCGTCGACGCCCCGTTCTAC
CGCGCCGACGCCGAGGTGATGGCCGAGGTCGTCGGCTTCCACCTGAATAGGGGTGTACTGGCGTCGGCGTCGCGGCCGCC
GGAGATCAGCGTGGCCGACGCGCTCGACGGCGCCCGCACCGTGGCGGTGCTCGAAGGTGTCAACGACCACGAGAACCTCG
GGTCGATCTTCCGCAACGCCGCGGGCCTCGGCGTCGACGCGGTGATCTTCGGGGCCGGATGTGCCGACCCGCTCTACCGG
CGGGCGGTGCGGGTGTCGATGGGCCATGCGCTGCTGGTGCCGTTCGCCCGCGCAGCCGCGTGGCCGGATGAGCTGAACAC
GTTGCGCGACAAGGGGTTTCAGTTGCTGGCGATGACGCCGGACCCCGCCGCCGACACCCTGGCCACGGCGATGACGGCGC
TGGCCGGCGAGAGGGTCGCCTTCCTCGTCGGCGCGGAGGGACCGGGCCTGACCGAGCGGGTGATGCGGGCCAGCGACCGG
CGGGTGCGCATCCCGATGTCGCGGGGCACCGATTCGCTCAACGTCGCGACCGCTGCCGCGCTGGCGTTCTACGAACGGGT
CCGATCCGGCTAG

Upstream 100 bases:

>100_bases
GAACCGGAGCAGCGCAAGACGTTGTTGATGGCCGCCGACCTGATGTCGGCGATGGTCGAGGCGAGCGCGTAAGCTTCGCC
GCCGTGCGGAGAGTGGGGCG

Downstream 100 bases:

>100_bases
ATTGACCCGGTGACCGACGAATCCACTCCGTGGGGAACGGGTTTGACAGTGGCCGCCGGAGTCGCGGCGGTGATCGCCAC
GGCCGTCATCGTGCTGAGCC

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MTSVIDVDDPADPRLDDFRDLNSIDRRPDLPSGKGLVIAEGVLVVQRMVASRFTPRALLGTDRRLTELGTDLDAVDAPFY
RADAEVMAEVVGFHLNRGVLASASRPPEISVADALDGARTVAVLEGVNDHENLGSIFRNAAGLGVDAVIFGAGCADPLYR
RAVRVSMGHALLVPFARAAAWPDELNTLRDKGFQLLAMTPDPAADTLATAMTALAGERVAFLVGAEGPGLTERVMRASDR
RVRIPMSRGTDSLNVATAAALAFYERVRSG

Sequences:

>Translated_270_residues
MTSVIDVDDPADPRLDDFRDLNSIDRRPDLPSGKGLVIAEGVLVVQRMVASRFTPRALLGTDRRLTELGTDLDAVDAPFY
RADAEVMAEVVGFHLNRGVLASASRPPEISVADALDGARTVAVLEGVNDHENLGSIFRNAAGLGVDAVIFGAGCADPLYR
RAVRVSMGHALLVPFARAAAWPDELNTLRDKGFQLLAMTPDPAADTLATAMTALAGERVAFLVGAEGPGLTERVMRASDR
RVRIPMSRGTDSLNVATAAALAFYERVRSG
>Mature_269_residues
TSVIDVDDPADPRLDDFRDLNSIDRRPDLPSGKGLVIAEGVLVVQRMVASRFTPRALLGTDRRLTELGTDLDAVDAPFYR
ADAEVMAEVVGFHLNRGVLASASRPPEISVADALDGARTVAVLEGVNDHENLGSIFRNAAGLGVDAVIFGAGCADPLYRR
AVRVSMGHALLVPFARAAAWPDELNTLRDKGFQLLAMTPDPAADTLATAMTALAGERVAFLVGAEGPGLTERVMRASDRR
VRIPMSRGTDSLNVATAAALAFYERVRSG

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=147, Percent_Identity=29.9319727891156, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28765; Mature: 28634

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVIDVDDPADPRLDDFRDLNSIDRRPDLPSGKGLVIAEGVLVVQRMVASRFTPRALLG
CCCEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHC
TDRRLTELGTDLDAVDAPFYRADAEVMAEVVGFHLNRGVLASASRPPEISVADALDGART
CCHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHCCCHHE
VAVLEGVNDHENLGSIFRNAAGLGVDAVIFGAGCADPLYRRAVRVSMGHALLVPFARAAA
EHHHCCCCCHHHHHHHHHHHCCCCCHHEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHC
WPDELNTLRDKGFQLLAMTPDPAADTLATAMTALAGERVAFLVGAEGPGLTERVMRASDR
CHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCC
RVRIPMSRGTDSLNVATAAALAFYERVRSG
EEEEECCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSVIDVDDPADPRLDDFRDLNSIDRRPDLPSGKGLVIAEGVLVVQRMVASRFTPRALLG
CCEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHC
TDRRLTELGTDLDAVDAPFYRADAEVMAEVVGFHLNRGVLASASRPPEISVADALDGART
CCHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHCCCHHE
VAVLEGVNDHENLGSIFRNAAGLGVDAVIFGAGCADPLYRRAVRVSMGHALLVPFARAAA
EHHHCCCCCHHHHHHHHHHHCCCCCHHEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHC
WPDELNTLRDKGFQLLAMTPDPAADTLATAMTALAGERVAFLVGAEGPGLTERVMRASDR
CHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCC
RVRIPMSRGTDSLNVATAAALAFYERVRSG
EEEEECCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]