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The map label for this gene is stp [H]

Identifier: 108801418

GI number: 108801418

Start: 4736811

End: 4738247

Strand: Reverse

Name: stp [H]

Synonym: Mmcs_4455

Alternate gene names: 108801418

Gene position: 4738247-4736811 (Counterclockwise)

Preceding gene: 108801419

Following gene: 108801417

Centisome position: 83.05

GC content: 68.34

Gene sequence:

>1437_bases
ATGAGCGCACGGCGCAAGGCCATCATCCTGGTCTCGTGCTGTCTGAGCCTGCTGATCGTTTCCATGGACGCCACGATCGT
CAACGTCGCGATCCCGGCGATCCGCTCGGATCTGCACGCCTCCTCGTCGCAGCTGCAGTGGGTGGTCGACGTCTACACCC
TGGTGCTGGCCTCGCTGTTGATCCTGGCGGGGGCGACCGGGGACCGGTTCGGCCGGCGCCGGGTGTTTCAGATCGGGCTG
ACCGTCTTCGCCTTCGGATCGCTGCTGTGCAGCCTCGCCCCCAACATCGAGACGCTGATCGTGGCGCGCCTGCTGCAGGC
CATCGGCGGTTCGATGATGAATCCCGTTGCGCTGTCAATCGTCTCGCAGATCTTCACCGGCCGGGTGGAACGGGCCCGGG
CGCTGGGCATCTGGGGTTCGGTGGTGGGCATCGCCATGTCGCTGGGCCCGATCGTCGGGGGTCTGCTCATCGAGACGGTC
AGTTGGCGGGCGGTCTTCTGGATCAACCTGCCGATCTGCGCGGCCGCGATCATCCTGACCGCGGTGTTCGTGCCCGAGAC
GAAGTCGGCGACCATGCGCAACATCGATCCGATCGGGCAGGCCCTGGCCGTGGTGTTCCTGTTCGGGATCGTGTTCGCCC
TGATCGAGGGGCCCGGTCTGGGCTGGACCGATCCGCTCACGCTCACCGCCGCCGTGGTTGCGCTGGTGGCGTTCGCGGGG
TTCCTCCGCTACGAATCACGCCGCCACGACCCCTTCATCGATCTGCGCTTCTTCCGCAGTATCCCGTTCAGCGCGGCGAC
GGTGACCGCGGTGTCGGCGTTCGCCGGCTGGGGTGCGTTCCTGTTCATGATGTCGCTGTACCTGCAGGGTGAGCGCGGCT
ACTCCGCGATGGAGACCGGTCTCATCTACTTGCCCATCGCGATCGGGGCACTGCTGTTCTCGCCGCTGTCGGGCCGGCTG
GTGGGGCGTTTCGGCGCCCGGCCGTCGCTGCTGATCGCCGGGGTGTTCATCACGGCGGCGTCGGCGATGCTGAGCTTCCT
CACCGCGGCCACCCCGGTCTGGGAACTGCTCGTCGTGTTCGCCGTCTTCGGTATCGGGTTCTCGATGGTCAACGCGCCGA
TCACCAATGCCGCGGTCAGCGGGATGCCGCTGGAGCGCGCGGGCGCGGCGTCGGCGGTGACGTCCACCAGCAGGCAGATC
GGCGTGAGTATCGGTGTGGCGCTGTGCGGTTCGGTCGCGGGCGGTGCGCTGGCCGGCACGTCGACGGACTTCGCCGCCGC
GGCGCGGCCGCTGTGGTTCGTCCTCGTCGGTTTCGGGCTGATCATCACCGCGCTGGCGGTGTACTCCACATCGGGCCGCG
CCATGCACTCCGCCGACCGGCTGGCGCCGCTGATCTCGGGAGATGTGGTGGGGCAGGGGGCGACCCGTGCCGCGTAA

Upstream 100 bases:

>100_bases
ACCGACAAGCGCTGACCTCGCCACTTCCGTCGATTTCGGCGCTGGTTTGCCTTGCGAACAGTTGTAGTTTCACAATGGTT
GGGTGATTGAAACCGTCGGC

Downstream 100 bases:

>100_bases
CCCGCTCGCCGACGAGGTATGGGGTGCGTTGACGGCCCTGGTCTTCGACAACCGCGACAGCTGGCGGCGCGCGGTGGTCG
AGGAGACGGGGTTGCCGTTC

Product: EmrB/QacA family drug resistance transporter

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSARRKAIILVSCCLSLLIVSMDATIVNVAIPAIRSDLHASSSQLQWVVDVYTLVLASLLILAGATGDRFGRRRVFQIGL
TVFAFGSLLCSLAPNIETLIVARLLQAIGGSMMNPVALSIVSQIFTGRVERARALGIWGSVVGIAMSLGPIVGGLLIETV
SWRAVFWINLPICAAAIILTAVFVPETKSATMRNIDPIGQALAVVFLFGIVFALIEGPGLGWTDPLTLTAAVVALVAFAG
FLRYESRRHDPFIDLRFFRSIPFSAATVTAVSAFAGWGAFLFMMSLYLQGERGYSAMETGLIYLPIAIGALLFSPLSGRL
VGRFGARPSLLIAGVFITAASAMLSFLTAATPVWELLVVFAVFGIGFSMVNAPITNAAVSGMPLERAGAASAVTSTSRQI
GVSIGVALCGSVAGGALAGTSTDFAAAARPLWFVLVGFGLIITALAVYSTSGRAMHSADRLAPLISGDVVGQGATRAA

Sequences:

>Translated_478_residues
MSARRKAIILVSCCLSLLIVSMDATIVNVAIPAIRSDLHASSSQLQWVVDVYTLVLASLLILAGATGDRFGRRRVFQIGL
TVFAFGSLLCSLAPNIETLIVARLLQAIGGSMMNPVALSIVSQIFTGRVERARALGIWGSVVGIAMSLGPIVGGLLIETV
SWRAVFWINLPICAAAIILTAVFVPETKSATMRNIDPIGQALAVVFLFGIVFALIEGPGLGWTDPLTLTAAVVALVAFAG
FLRYESRRHDPFIDLRFFRSIPFSAATVTAVSAFAGWGAFLFMMSLYLQGERGYSAMETGLIYLPIAIGALLFSPLSGRL
VGRFGARPSLLIAGVFITAASAMLSFLTAATPVWELLVVFAVFGIGFSMVNAPITNAAVSGMPLERAGAASAVTSTSRQI
GVSIGVALCGSVAGGALAGTSTDFAAAARPLWFVLVGFGLIITALAVYSTSGRAMHSADRLAPLISGDVVGQGATRAA
>Mature_477_residues
SARRKAIILVSCCLSLLIVSMDATIVNVAIPAIRSDLHASSSQLQWVVDVYTLVLASLLILAGATGDRFGRRRVFQIGLT
VFAFGSLLCSLAPNIETLIVARLLQAIGGSMMNPVALSIVSQIFTGRVERARALGIWGSVVGIAMSLGPIVGGLLIETVS
WRAVFWINLPICAAAIILTAVFVPETKSATMRNIDPIGQALAVVFLFGIVFALIEGPGLGWTDPLTLTAAVVALVAFAGF
LRYESRRHDPFIDLRFFRSIPFSAATVTAVSAFAGWGAFLFMMSLYLQGERGYSAMETGLIYLPIAIGALLFSPLSGRLV
GRFGARPSLLIAGVFITAASAMLSFLTAATPVWELLVVFAVFGIGFSMVNAPITNAAVSGMPLERAGAASAVTSTSRQIG
VSIGVALCGSVAGGALAGTSTDFAAAARPLWFVLVGFGLIITALAVYSTSGRAMHSADRLAPLISGDVVGQGATRAA

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=395, Percent_Identity=30.6329113924051, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1790195, Length=406, Percent_Identity=28.5714285714286, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788710, Length=412, Percent_Identity=29.126213592233, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789042, Length=452, Percent_Identity=26.9911504424779, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788392, Length=402, Percent_Identity=29.8507462686567, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1790146, Length=162, Percent_Identity=30.8641975308642, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI48994889, Length=166, Percent_Identity=31.3253012048193, Blast_Score=66, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324954, Length=451, Percent_Identity=23.2815964523282, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6323735, Length=320, Percent_Identity=27.8125, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6322958, Length=393, Percent_Identity=23.6641221374046, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323520, Length=428, Percent_Identity=22.4299065420561, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=137, Percent_Identity=34.3065693430657, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=317, Percent_Identity=25.2365930599369, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49880; Mature: 49748

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARRKAIILVSCCLSLLIVSMDATIVNVAIPAIRSDLHASSSQLQWVVDVYTLVLASLL
CCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ILAGATGDRFGRRRVFQIGLTVFAFGSLLCSLAPNIETLIVARLLQAIGGSMMNPVALSI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHH
VSQIFTGRVERARALGIWGSVVGIAMSLGPIVGGLLIETVSWRAVFWINLPICAAAIILT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHHHHHHH
AVFVPETKSATMRNIDPIGQALAVVFLFGIVFALIEGPGLGWTDPLTLTAAVVALVAFAG
HHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
FLRYESRRHDPFIDLRFFRSIPFSAATVTAVSAFAGWGAFLFMMSLYLQGERGYSAMETG
HHHHHCCCCCCCEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCC
LIYLPIAIGALLFSPLSGRLVGRFGARPSLLIAGVFITAASAMLSFLTAATPVWELLVVF
HHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVFGIGFSMVNAPITNAAVSGMPLERAGAASAVTSTSRQIGVSIGVALCGSVAGGALAGT
HHHHHHHHHHCCCHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
STDFAAAARPLWFVLVGFGLIITALAVYSTSGRAMHSADRLAPLISGDVVGQGATRAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SARRKAIILVSCCLSLLIVSMDATIVNVAIPAIRSDLHASSSQLQWVVDVYTLVLASLL
CCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ILAGATGDRFGRRRVFQIGLTVFAFGSLLCSLAPNIETLIVARLLQAIGGSMMNPVALSI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHH
VSQIFTGRVERARALGIWGSVVGIAMSLGPIVGGLLIETVSWRAVFWINLPICAAAIILT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHHHHHHH
AVFVPETKSATMRNIDPIGQALAVVFLFGIVFALIEGPGLGWTDPLTLTAAVVALVAFAG
HHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
FLRYESRRHDPFIDLRFFRSIPFSAATVTAVSAFAGWGAFLFMMSLYLQGERGYSAMETG
HHHHHCCCCCCCEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCC
LIYLPIAIGALLFSPLSGRLVGRFGARPSLLIAGVFITAASAMLSFLTAATPVWELLVVF
HHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVFGIGFSMVNAPITNAAVSGMPLERAGAASAVTSTSRQIGVSIGVALCGSVAGGALAGT
HHHHHHHHHHCCCHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
STDFAAAARPLWFVLVGFGLIITALAVYSTSGRAMHSADRLAPLISGDVVGQGATRAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]