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The map label for this gene is yfkH [H]

Identifier: 108801303

GI number: 108801303

Start: 4615212

End: 4616264

Strand: Reverse

Name: yfkH [H]

Synonym: Mmcs_4339

Alternate gene names: 108801303

Gene position: 4616264-4615212 (Counterclockwise)

Preceding gene: 108801304

Following gene: 108801302

Centisome position: 80.91

GC content: 68.85

Gene sequence:

>1053_bases
ATGGCCGAAGCTGCGTCCACGAAACACCGCGCACCCGACCCTGACGATCCGCGCAAACCGGAGTCTCCGACCGACCTGAC
CAAACCGTCGGCGTTCTACGTGCTGCGCAAGACCGCCCGGGAGTTCGGCCACGATCAGTGCACCGACCTGGCCGCCGCGC
TCACCTACTACGCCGTCCTGTCGCTGTTCCCGGCGGTGCTGGTGATGGTGTCGCTGCTCGGCGTCTTCGGCCAGGGCCGC
CGCACCACCGACGCCCTGCTCGACATCGTCTCGGACGTCGCCCCGGCCTCGACGGTCGACACCCTGCGGCCCACCATCGA
GCAACTCGTGGAATCACCGTCTGCCGGTTTCGCTCTGGTGATCGGCATCCTCACCGCGCTGTGGTCGGCCTCCGGGTATG
TCGGCGCGTTCGGACGGGCGATGAACCGGATCTACGAGGTCGCCGAGGGCCGCCCGGTGTGGAAGCTGCGGCCGCTGCAA
CTCGTCCTGACCTTCGCCGCGCTGCTCGGCGCGGCGTTGGTGGCGTTCATGCTCGCGGTGAGCGGTCCGGTGGCCGAAGC
CCTCGGCAACGCGATCGGTCTCGGCGAGGCGGCGGTGACCGCGTGGAGTATCGGGCGTTGGCCGGTGATCCTGCTGCTCG
TCGTCGTCGCCGTCGCGGTGCTGTACTACGCCACTCCCAACGTGCAGCAGCCCAAGTTCCGGTGGATCAGCCTCGGCGCC
GGGCTGGCGATCCTGACCTGGATCGTGGCGTCGGTGGGGTTCGGGTTCTACGTCGCGAACTTCAGCAGCTACAACAAGAC
CTACGGGACGCTGGCCGGGGTGATCATCTTCCTGCTGTGGCTGTGGATCACCAACCTCGCGTTGCTCTTCGGCGCCGAAC
TGGACGCCGAACTCGAACGGGGCCGCCAGCTGCAGGCCGGCCTGCCCGCCGAGCGGGAACTGCAGCTGCCCCCGCGCGAC
ACCCGGGTCATCGAGAAGAAGGAAGCCGCCATCGAAGAGGACGTCGAACGCGCGAAGGCGCTGCGACGCAGCCGGGGCCG
CGACGACGGGTGA

Upstream 100 bases:

>100_bases
GGCCCGAACGTGAGCCTGTGCCTGGGAATTCGGCCGAATCTCGAACACAGCTCACGTTCGCGCGAGTTTGGGACACGGCC
GTCCGGGTATCGACTGAGGC

Downstream 100 bases:

>100_bases
CCCACGCACCCGGGCATACTCACCTGCGTGACTCGACGGATCCACGTCATCGGCATCGGTGCGGGTGACCCGGACTACGT
GACCGCCCAGGCCGTCGCGG

Product: ribonuclease BN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MAEAASTKHRAPDPDDPRKPESPTDLTKPSAFYVLRKTAREFGHDQCTDLAAALTYYAVLSLFPAVLVMVSLLGVFGQGR
RTTDALLDIVSDVAPASTVDTLRPTIEQLVESPSAGFALVIGILTALWSASGYVGAFGRAMNRIYEVAEGRPVWKLRPLQ
LVLTFAALLGAALVAFMLAVSGPVAEALGNAIGLGEAAVTAWSIGRWPVILLLVVVAVAVLYYATPNVQQPKFRWISLGA
GLAILTWIVASVGFGFYVANFSSYNKTYGTLAGVIIFLLWLWITNLALLFGAELDAELERGRQLQAGLPAERELQLPPRD
TRVIEKKEAAIEEDVERAKALRRSRGRDDG

Sequences:

>Translated_350_residues
MAEAASTKHRAPDPDDPRKPESPTDLTKPSAFYVLRKTAREFGHDQCTDLAAALTYYAVLSLFPAVLVMVSLLGVFGQGR
RTTDALLDIVSDVAPASTVDTLRPTIEQLVESPSAGFALVIGILTALWSASGYVGAFGRAMNRIYEVAEGRPVWKLRPLQ
LVLTFAALLGAALVAFMLAVSGPVAEALGNAIGLGEAAVTAWSIGRWPVILLLVVVAVAVLYYATPNVQQPKFRWISLGA
GLAILTWIVASVGFGFYVANFSSYNKTYGTLAGVIIFLLWLWITNLALLFGAELDAELERGRQLQAGLPAERELQLPPRD
TRVIEKKEAAIEEDVERAKALRRSRGRDDG
>Mature_349_residues
AEAASTKHRAPDPDDPRKPESPTDLTKPSAFYVLRKTAREFGHDQCTDLAAALTYYAVLSLFPAVLVMVSLLGVFGQGRR
TTDALLDIVSDVAPASTVDTLRPTIEQLVESPSAGFALVIGILTALWSASGYVGAFGRAMNRIYEVAEGRPVWKLRPLQL
VLTFAALLGAALVAFMLAVSGPVAEALGNAIGLGEAAVTAWSIGRWPVILLLVVVAVAVLYYATPNVQQPKFRWISLGAG
LAILTWIVASVGFGFYVANFSSYNKTYGTLAGVIIFLLWLWITNLALLFGAELDAELERGRQLQAGLPAERELQLPPRDT
RVIEKKEAAIEEDVERAKALRRSRGRDDG

Specific function: 3' To 5' Exoribonuclease Necessary For 3'Maturation Of tRNA Molecules. [C]

COG id: COG1295

COG function: function code S; Predicted membrane protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790319, Length=283, Percent_Identity=28.6219081272085, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017039
- InterPro:   IPR004664 [H]

Pfam domain/function: PF03631 Ribonuclease_BN [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 37824; Mature: 37693

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAASTKHRAPDPDDPRKPESPTDLTKPSAFYVLRKTAREFGHDQCTDLAAALTYYAVL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
SLFPAVLVMVSLLGVFGQGRRTTDALLDIVSDVAPASTVDTLRPTIEQLVESPSAGFALV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHH
IGILTALWSASGYVGAFGRAMNRIYEVAEGRPVWKLRPLQLVLTFAALLGAALVAFMLAV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
SGPVAEALGNAIGLGEAAVTAWSIGRWPVILLLVVVAVAVLYYATPNVQQPKFRWISLGA
CCHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEECCC
GLAILTWIVASVGFGFYVANFSSYNKTYGTLAGVIIFLLWLWITNLALLFGAELDAELER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHC
GRQLQAGLPAERELQLPPRDTRVIEKKEAAIEEDVERAKALRRSRGRDDG
CCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AEAASTKHRAPDPDDPRKPESPTDLTKPSAFYVLRKTAREFGHDQCTDLAAALTYYAVL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
SLFPAVLVMVSLLGVFGQGRRTTDALLDIVSDVAPASTVDTLRPTIEQLVESPSAGFALV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHH
IGILTALWSASGYVGAFGRAMNRIYEVAEGRPVWKLRPLQLVLTFAALLGAALVAFMLAV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
SGPVAEALGNAIGLGEAAVTAWSIGRWPVILLLVVVAVAVLYYATPNVQQPKFRWISLGA
CCHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEECCC
GLAILTWIVASVGFGFYVANFSSYNKTYGTLAGVIIFLLWLWITNLALLFGAELDAELER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHC
GRQLQAGLPAERELQLPPRDTRVIEKKEAAIEEDVERAKALRRSRGRDDG
CCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]