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The map label for this gene is fadD [C]

Identifier: 108801225

GI number: 108801225

Start: 4526325

End: 4527959

Strand: Reverse

Name: fadD [C]

Synonym: Mmcs_4261

Alternate gene names: 108801225

Gene position: 4527959-4526325 (Counterclockwise)

Preceding gene: 108801226

Following gene: 108801224

Centisome position: 79.36

GC content: 67.95

Gene sequence:

>1635_bases
GTGAGCAGGTTCACCGAGAAGATGTACCGCAACGCGCGAACCGTGAGCACCGGCATGGTGACCGGCGAGCCGCACGAACC
CATCCGCCACACCTGGGGTGAGGTGCACGAGCGGGCCCGCCGCATCGCCAGCGGACTCGCGGCAGCCGGTGTCGGCCACG
GCGACGCCGTGGGTGTGCTGGCCGGCTTCCCGGTCGAGATCGCCCCGACCGCGCAGGGGCTGTGGATGCGCGGCGCGAGC
CTGACCATGCTGCATCAGCCGACACCGCGCACCGACCTCGCGATGTGGGCCGAAGACACGATGAACGTCATCGGCATGAT
CGAGGCGAAGGCCGTCATCGTCTCCGAGCCGTTCGTGGTCGCCATCCCGGTGCTCGAGGAGAAGGGGATCAAGGTCCTCA
CCGTCAGTGACCTGCTGGCGGCCGAGCCGATCGACCCCATCGAGGTGGGCGAGGACGATCTGGCGCTGATGCAGCTGACG
TCGGGATCGACCGGATCCCCGAAGGCCGTGCAGGTGACGCACCGCAACATCCACTCCAACGCCGAGGCGATGTTCATCGG
CGCGGAGTACGACGTCGAGAACGACGTCATGGTCAGCTGGCTGCCCTGTTTCCACGACATGGGCATGGTCGGCTTCCTCA
CCATCCCGATGTACTTCGGCGCCGAGCTCGTCAAGGTCACGCCGATGGACTTCCTGCGCGACACCCTGCTGTGGGCCAAG
CTCATCGACAAGTACAAGGGCACGATGACGGCGGCGCCGAACTTCGCCTATGCGCTGCTGGCCAAGCGGCTGCGCCGCCA
GGCCAAACCGGGCGACTTCGACCTGTCCACGCTGCGATTCGCGCTCTCGGGCGCCGAGCCGGTCGAACCCGCCGACGTCG
AGGATCTGATCGACGCCGGAAAGCCGTTCGGCCTCAAGCCGGAAGCCATCCTGCCCGCATACGGCATGGCCGAGACCACG
CTCGCCGTGTCGTTCTCGCCGTGCGGCGCCGGTCTCGTCGTCGACGAGGTCGACGCCGACCTGCTGGCTGCGCTGCGCCG
CGCCGTGCCCGCCACCAAGGGCAACACCAAGCGGCTGGCCGATCTGGGCCCGCTGCTCACCGATCTCGAAGCCCGCGTCA
TCGACGAGAACGGCAATGTCATGCCGCCTCGCGGAGTGGGCGTCATCGAACTGCGCGGCGAATGCGTCACGCCCGGGTAC
ATGACCATGGGTGGATTCATCCCGGCCCAGGACGACCACGGCTGGTACGACACCGGCGACCTCGGCTACATCACCGAGGA
GGGTCACGTCGTGGTCTGCGGCCGCGTCAAGGACGTGATCATCATGGCCGGGCGCAACATCTACCCGACCGACATCGAAC
GCGCGGCGGGCCGCGTCGAGGGTGTCCGGCCGGGTTGTGCCGTCGCGGTGCGCCTCGATGCCGGACACTCGCGTGAGACG
TTCGCCGTGGCGGTGGAGTCCAACAACTGGCAGGATCCCGCCGAGGTGCGCCGCATCGAGCATCAGGTCGCCCACGAGGT
CGTGGCCGAGGTCGAGATGCGTCCACGCAATGTCGTGGTTCTCGGACCGGGCAGCATCCCGAAGACCTCGTCGGGCAAGC
TGCGCCGCGCCAACTCGGTCTCGCTGGTCACCTAA

Upstream 100 bases:

>100_bases
CAACTGCTCCTGAATCGTGTGCGCGTGCCTACTCGGCAACGCTCGTGGACAGGCGGAGCATTAAAAGCCGGGCAGTGGCG
CCGGTCCACAGGAGGATGTC

Downstream 100 bases:

>100_bases
TCCTGCCGAGCAGACGCAAAAGTCCCCGAAACGTCGGCGTGTTGCGGACATTTGTGTCTGCTCGCGCACCGAAACTCGTT
CGCCCGCTGTCGGTCGGCGC

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MSRFTEKMYRNARTVSTGMVTGEPHEPIRHTWGEVHERARRIASGLAAAGVGHGDAVGVLAGFPVEIAPTAQGLWMRGAS
LTMLHQPTPRTDLAMWAEDTMNVIGMIEAKAVIVSEPFVVAIPVLEEKGIKVLTVSDLLAAEPIDPIEVGEDDLALMQLT
SGSTGSPKAVQVTHRNIHSNAEAMFIGAEYDVENDVMVSWLPCFHDMGMVGFLTIPMYFGAELVKVTPMDFLRDTLLWAK
LIDKYKGTMTAAPNFAYALLAKRLRRQAKPGDFDLSTLRFALSGAEPVEPADVEDLIDAGKPFGLKPEAILPAYGMAETT
LAVSFSPCGAGLVVDEVDADLLAALRRAVPATKGNTKRLADLGPLLTDLEARVIDENGNVMPPRGVGVIELRGECVTPGY
MTMGGFIPAQDDHGWYDTGDLGYITEEGHVVVCGRVKDVIIMAGRNIYPTDIERAAGRVEGVRPGCAVAVRLDAGHSRET
FAVAVESNNWQDPAEVRRIEHQVAHEVVAEVEMRPRNVVVLGPGSIPKTSSGKLRRANSVSLVT

Sequences:

>Translated_544_residues
MSRFTEKMYRNARTVSTGMVTGEPHEPIRHTWGEVHERARRIASGLAAAGVGHGDAVGVLAGFPVEIAPTAQGLWMRGAS
LTMLHQPTPRTDLAMWAEDTMNVIGMIEAKAVIVSEPFVVAIPVLEEKGIKVLTVSDLLAAEPIDPIEVGEDDLALMQLT
SGSTGSPKAVQVTHRNIHSNAEAMFIGAEYDVENDVMVSWLPCFHDMGMVGFLTIPMYFGAELVKVTPMDFLRDTLLWAK
LIDKYKGTMTAAPNFAYALLAKRLRRQAKPGDFDLSTLRFALSGAEPVEPADVEDLIDAGKPFGLKPEAILPAYGMAETT
LAVSFSPCGAGLVVDEVDADLLAALRRAVPATKGNTKRLADLGPLLTDLEARVIDENGNVMPPRGVGVIELRGECVTPGY
MTMGGFIPAQDDHGWYDTGDLGYITEEGHVVVCGRVKDVIIMAGRNIYPTDIERAAGRVEGVRPGCAVAVRLDAGHSRET
FAVAVESNNWQDPAEVRRIEHQVAHEVVAEVEMRPRNVVVLGPGSIPKTSSGKLRRANSVSLVT
>Mature_543_residues
SRFTEKMYRNARTVSTGMVTGEPHEPIRHTWGEVHERARRIASGLAAAGVGHGDAVGVLAGFPVEIAPTAQGLWMRGASL
TMLHQPTPRTDLAMWAEDTMNVIGMIEAKAVIVSEPFVVAIPVLEEKGIKVLTVSDLLAAEPIDPIEVGEDDLALMQLTS
GSTGSPKAVQVTHRNIHSNAEAMFIGAEYDVENDVMVSWLPCFHDMGMVGFLTIPMYFGAELVKVTPMDFLRDTLLWAKL
IDKYKGTMTAAPNFAYALLAKRLRRQAKPGDFDLSTLRFALSGAEPVEPADVEDLIDAGKPFGLKPEAILPAYGMAETTL
AVSFSPCGAGLVVDEVDADLLAALRRAVPATKGNTKRLADLGPLLTDLEARVIDENGNVMPPRGVGVIELRGECVTPGYM
TMGGFIPAQDDHGWYDTGDLGYITEEGHVVVCGRVKDVIIMAGRNIYPTDIERAAGRVEGVRPGCAVAVRLDAGHSRETF
AVAVESNNWQDPAEVRRIEHQVAHEVVAEVEMRPRNVVVLGPGSIPKTSSGKLRRANSVSLVT

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=475, Percent_Identity=23.3684210526316, Blast_Score=90, Evalue=5e-18,
Organism=Escherichia coli, GI1788107, Length=397, Percent_Identity=25.9445843828715, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1789201, Length=390, Percent_Identity=27.9487179487179, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI145693145, Length=389, Percent_Identity=27.5064267352185, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17560308, Length=326, Percent_Identity=25.7668711656442, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17558820, Length=399, Percent_Identity=24.3107769423559, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71994703, Length=483, Percent_Identity=23.6024844720497, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994690, Length=483, Percent_Identity=23.6024844720497, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994694, Length=483, Percent_Identity=23.6024844720497, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=409, Percent_Identity=24.2053789731051, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17559526, Length=375, Percent_Identity=24.5333333333333, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=307, Percent_Identity=26.7100977198697, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI18859661, Length=324, Percent_Identity=25, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24581924, Length=308, Percent_Identity=24.3506493506494, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI20130357, Length=513, Percent_Identity=23.1968810916179, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648257, Length=419, Percent_Identity=21.0023866348449, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 58661; Mature: 58530

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRFTEKMYRNARTVSTGMVTGEPHEPIRHTWGEVHERARRIASGLAAAGVGHGDAVGVL
CCHHHHHHHHCCCEEEECEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
AGFPVEIAPTAQGLWMRGASLTMLHQPTPRTDLAMWAEDTMNVIGMIEAKAVIVSEPFVV
ECCCEEECCCCCCEEECCCEEEEEECCCCCCHHHHHHHHHHHHHEEEEEEEEEEECCEEE
AIPVLEEKGIKVLTVSDLLAAEPIDPIEVGEDDLALMQLTSGSTGSPKAVQVTHRNIHSN
EEEEECCCCCEEEEEHHHHHCCCCCHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCCCC
AEAMFIGAEYDVENDVMVSWLPCFHDMGMVGFLTIPMYFGAELVKVTPMDFLRDTLLWAK
CCEEEEEEECCCCCCEEEEEEHHHHHCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHH
LIDKYKGTMTAAPNFAYALLAKRLRRQAKPGDFDLSTLRFALSGAEPVEPADVEDLIDAG
HHHHHCCCEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHEECCCCCCCCCCHHHHHHCC
KPFGLKPEAILPAYGMAETTLAVSFSPCGAGLVVDEVDADLLAALRRAVPATKGNTKRLA
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEECCCHHHHHHHHHHCCCCCCCCHHHH
DLGPLLTDLEARVIDENGNVMPPRGVGVIELRGECVTPGYMTMGGFIPAQDDHGWYDTGD
HHHHHHHCCEEEEECCCCCCCCCCCCEEEEECCCEECCCCHHHCCCCCCCCCCCCCCCCC
LGYITEEGHVVVCGRVKDVIIMAGRNIYPTDIERAAGRVEGVRPGCAVAVRLDAGHSRET
CCEEECCCCEEEECCCCEEEEEECCCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCE
FAVAVESNNWQDPAEVRRIEHQVAHEVVAEVEMRPRNVVVLGPGSIPKTSSGKLRRANSV
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEECCCE
SLVT
EEEC
>Mature Secondary Structure 
SRFTEKMYRNARTVSTGMVTGEPHEPIRHTWGEVHERARRIASGLAAAGVGHGDAVGVL
CHHHHHHHHCCCEEEECEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
AGFPVEIAPTAQGLWMRGASLTMLHQPTPRTDLAMWAEDTMNVIGMIEAKAVIVSEPFVV
ECCCEEECCCCCCEEECCCEEEEEECCCCCCHHHHHHHHHHHHHEEEEEEEEEEECCEEE
AIPVLEEKGIKVLTVSDLLAAEPIDPIEVGEDDLALMQLTSGSTGSPKAVQVTHRNIHSN
EEEEECCCCCEEEEEHHHHHCCCCCHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCCCC
AEAMFIGAEYDVENDVMVSWLPCFHDMGMVGFLTIPMYFGAELVKVTPMDFLRDTLLWAK
CCEEEEEEECCCCCCEEEEEEHHHHHCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHH
LIDKYKGTMTAAPNFAYALLAKRLRRQAKPGDFDLSTLRFALSGAEPVEPADVEDLIDAG
HHHHHCCCEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHEECCCCCCCCCCHHHHHHCC
KPFGLKPEAILPAYGMAETTLAVSFSPCGAGLVVDEVDADLLAALRRAVPATKGNTKRLA
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEECCCHHHHHHHHHHCCCCCCCCHHHH
DLGPLLTDLEARVIDENGNVMPPRGVGVIELRGECVTPGYMTMGGFIPAQDDHGWYDTGD
HHHHHHHCCEEEEECCCCCCCCCCCCEEEEECCCEECCCCHHHCCCCCCCCCCCCCCCCC
LGYITEEGHVVVCGRVKDVIIMAGRNIYPTDIERAAGRVEGVRPGCAVAVRLDAGHSRET
CCEEECCCCEEEECCCCEEEEEECCCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCE
FAVAVESNNWQDPAEVRRIEHQVAHEVVAEVEMRPRNVVVLGPGSIPKTSSGKLRRANSV
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEECCCE
SLVT
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA