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The map label for this gene is ispE [H]

Identifier: 108801226

GI number: 108801226

Start: 4528288

End: 4529256

Strand: Reverse

Name: ispE [H]

Synonym: Mmcs_4262

Alternate gene names: 108801226

Gene position: 4529256-4528288 (Counterclockwise)

Preceding gene: 108801227

Following gene: 108801225

Centisome position: 79.38

GC content: 72.65

Gene sequence:

>969_bases
GTGCGCAACGGCAACACCGCCTCCGAGTGGGTGCCCACCGGGTCGGTCACCGTGCGGGTGCCCGGCAAGGTCAACCTGTT
CCTGGGCGTCGGTGATCTGCGCGACGACGGCTACCACGATCTGACGACGGTCTTCCACGCCGTATCCCTGCTCGACGAGG
TCACCGTCTCGACCGCCGACACGCTGTCGCTGGATCTGGCCGGCGAGGGCGCCGAGTCGCTGCCCACCGATCGTCGCAAC
CTCGCCTGGAAAGCGGCCGAACTGATGGCCGAGCACGTCGGGAGATCACCCGATGTGGCGATCTCGATCGAGAAGTCGAT
TCCGGTGGCAGGCGGCATGGCCGGGGGCAGCGCCGACGCCGCGGGGGTGCTGGTCGCGATGAATCACCTCTGGGAGATCG
GCGTACCGCGACGCGATCTGCACGCGATGGCCGCCGAACTGGGCAGTGACGTCCCGTTCGCGCTGCACGGCGGTACCGCA
CTGGGCACCGGTCGCGGCGAGGAGCTCGCGACCGTACTGGCCCGCAGCACGTTCCACTGGGTGCTCGCCTTCGCGTACAA
GGGCCTCTCGACGCCGAAGGTGTTTCGTGAGCTCGACCGGTTGCGTGCCACGGCCGGCGCCGCCAGGGCCGAACCCGCCC
GGCTGGGCGATCCGGAGCCGATGCTGGCCGCGCTGGCGTCCGGGGATGCCCGGCAACTGGCGCCGCTGCTCGGCAACGAC
CTGCAGGCGGCCGCGCTGAGCCTGAGCCCGGACCTGCGGCGGACGCTGCGGGCCGGGCTGGACGCGGGTGCGCTGGCCGC
GATCGTGTCCGGGTCGGGGCCGACGTGCGCGTTCCTGTGCGCCTCGTCGCCGCAGGCGATCGACGTCGGCGCCCGCCTGG
CCGGTGCCGGGGTGTGCCGGACCGTGCGGGTGGCCAGTGGACCCGTGCAGGGTGCGCGGGTGGTCCCCGCACCCTCGCCG
TCGGTGTGA

Upstream 100 bases:

>100_bases
AGCGCTCCGGAGAAGCCGAGGAACAGGTCCGCGTCCAGTAGTGCCGGTCTGGCCTGCCGCTGGAGTTTGCACCGTCCACA
TGCGGCGATAGTGTCGAGCG

Downstream 100 bases:

>100_bases
GGATGAGGTCATTGCGTCCGGAAAGTGTGACGCACGCCTCAATCGGCCCGAGAGTTTGGCAGTTACTTAAGGGTCTCTTA
AGATGGTCGGCGGTGAAAGC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MRNGNTASEWVPTGSVTVRVPGKVNLFLGVGDLRDDGYHDLTTVFHAVSLLDEVTVSTADTLSLDLAGEGAESLPTDRRN
LAWKAAELMAEHVGRSPDVAISIEKSIPVAGGMAGGSADAAGVLVAMNHLWEIGVPRRDLHAMAAELGSDVPFALHGGTA
LGTGRGEELATVLARSTFHWVLAFAYKGLSTPKVFRELDRLRATAGAARAEPARLGDPEPMLAALASGDARQLAPLLGND
LQAAALSLSPDLRRTLRAGLDAGALAAIVSGSGPTCAFLCASSPQAIDVGARLAGAGVCRTVRVASGPVQGARVVPAPSP
SV

Sequences:

>Translated_322_residues
MRNGNTASEWVPTGSVTVRVPGKVNLFLGVGDLRDDGYHDLTTVFHAVSLLDEVTVSTADTLSLDLAGEGAESLPTDRRN
LAWKAAELMAEHVGRSPDVAISIEKSIPVAGGMAGGSADAAGVLVAMNHLWEIGVPRRDLHAMAAELGSDVPFALHGGTA
LGTGRGEELATVLARSTFHWVLAFAYKGLSTPKVFRELDRLRATAGAARAEPARLGDPEPMLAALASGDARQLAPLLGND
LQAAALSLSPDLRRTLRAGLDAGALAAIVSGSGPTCAFLCASSPQAIDVGARLAGAGVCRTVRVASGPVQGARVVPAPSP
SV
>Mature_322_residues
MRNGNTASEWVPTGSVTVRVPGKVNLFLGVGDLRDDGYHDLTTVFHAVSLLDEVTVSTADTLSLDLAGEGAESLPTDRRN
LAWKAAELMAEHVGRSPDVAISIEKSIPVAGGMAGGSADAAGVLVAMNHLWEIGVPRRDLHAMAAELGSDVPFALHGGTA
LGTGRGEELATVLARSTFHWVLAFAYKGLSTPKVFRELDRLRATAGAARAEPARLGDPEPMLAALASGDARQLAPLLGND
LQAAALSLSPDLRRTLRAGLDAGALAAIVSGSGPTCAFLCASSPQAIDVGARLAGAGVCRTVRVASGPVQGARVVPAPSP
SV

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=194, Percent_Identity=37.6288659793814, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1786184, Length=254, Percent_Identity=29.1338582677165, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 33000; Mature: 33000

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNGNTASEWVPTGSVTVRVPGKVNLFLGVGDLRDDGYHDLTTVFHAVSLLDEVTVSTAD
CCCCCCCHHEECCCCEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
TLSLDLAGEGAESLPTDRRNLAWKAAELMAEHVGRSPDVAISIEKSIPVAGGMAGGSADA
EEEEEECCCCHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCH
AGVLVAMNHLWEIGVPRRDLHAMAAELGSDVPFALHGGTALGTGRGEELATVLARSTFHW
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCEECCCCCHHHHHHHHHHHHHH
VLAFAYKGLSTPKVFRELDRLRATAGAARAEPARLGDPEPMLAALASGDARQLAPLLGND
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHCCC
LQAAALSLSPDLRRTLRAGLDAGALAAIVSGSGPTCAFLCASSPQAIDVGARLAGAGVCR
HHHHHCCCCHHHHHHHHHCCCCCHHEEEECCCCCEEEEEECCCCCEEECCHHHHCCCHHE
TVRVASGPVQGARVVPAPSPSV
EEEECCCCCCCCEECCCCCCCC
>Mature Secondary Structure
MRNGNTASEWVPTGSVTVRVPGKVNLFLGVGDLRDDGYHDLTTVFHAVSLLDEVTVSTAD
CCCCCCCHHEECCCCEEEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
TLSLDLAGEGAESLPTDRRNLAWKAAELMAEHVGRSPDVAISIEKSIPVAGGMAGGSADA
EEEEEECCCCHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCH
AGVLVAMNHLWEIGVPRRDLHAMAAELGSDVPFALHGGTALGTGRGEELATVLARSTFHW
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCEECCCCCHHHHHHHHHHHHHH
VLAFAYKGLSTPKVFRELDRLRATAGAARAEPARLGDPEPMLAALASGDARQLAPLLGND
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHCCC
LQAAALSLSPDLRRTLRAGLDAGALAAIVSGSGPTCAFLCASSPQAIDVGARLAGAGVCR
HHHHHCCCCHHHHHHHHHCCCCCHHEEEECCCCCEEEEEECCCCCEEECCHHHHCCCHHE
TVRVASGPVQGARVVPAPSPSV
EEEECCCCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA