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The map label for this gene is lcfB [H]

Identifier: 108800984

GI number: 108800984

Start: 4283703

End: 4285106

Strand: Reverse

Name: lcfB [H]

Synonym: Mmcs_4020

Alternate gene names: 108800984

Gene position: 4285106-4283703 (Counterclockwise)

Preceding gene: 108800989

Following gene: 108800983

Centisome position: 75.11

GC content: 71.37

Gene sequence:

>1404_bases
GTGTTGCTGACCTCGCTGAACCCCGCCGCGGTCGCCGCAGGCGCCGATATCGCCGACGCCGTCACGATCGGGGAGGCGGT
GCTCAGCCGTAGCGATCTGGTCGGGGCGGCCACGTCGGTCGCCGAGCGGGTCGGCGGTGCGCAGCGGGTCGCGGTGCTGG
CAACGCCGACGCCCGCGACGGTGTTGGCGATCACCGGCTGCCTGATCGCCGGCGTGCCCGTCGTGCCCGTGCCCGCCGAC
GTGGGTGTGGCCGAACGCCGCCACATCCTCACCGATTCCGGCGCGCAGGCGTGGCTCGGCGACAAGCCCGAGGACACTGA
GCTGCCGCACATCCCCGTCCGCCTGCACGCCCGGTCCTGGCACCGCTACGCCGAACCGCATCCCGACTCGGCCGCGCTGA
TCGTCTACACCTCCGGCACGACGGGCCCGCCCAAGGGCGTCGTCATCAGCCGCCGCGCGATCGCGGCCGACCTCGATGCG
CTCGCCGAGGCGTGGCAGTGGACCGCCGAGGACACCCTCGTCCACGGGTTGCCGCTCTACCACGTGCACGGGCTGGTGCT
CGGACTTCTCGGTTCGCTGCGCATCGGGAACCGGTTCGTCCACACCGGTCGGCCCACCCCCGAGGCCTACGCACAGGCGC
AGGGCACGCTGTACTTCGGGGTGCCGACGGTCTGGTCCCGCATCGCCGCCGACGACGACGCCGCCCGCGCACTGGCGACG
GCGCGGCTGCTCGTCTCGGGCAGTGCGCCGCTGCCGGTCCCGGTGTTCGACCGGTTGGCGGCGTCGACGGGCCACGAGGT
GGTCGAGCGCTACGGCAGCACCGAATCGTTGATCACCCTGAGCACCCGCGCGGACGGGGAGCGCCGGCCCGGCTGGGTGG
GACTCCCGCTGGCCGGGGTGGAGACCCGCCTGGTCGGCGACGACGGCGCACCGGTGCCCCACGACGGCGAAACCATTGCG
CGCCTAGAGGTTCGCGGCCCGACGTTGTTCGACGGATATCTGAACCGGCCGGATGCGACGGCCGATGTGCTGGGTGTGGA
CGGTTGGTACCGCACCGGTGACGTCGCTGTTGTCGACGGGGTTGGTATGCACCGCATCGTCGGCCGTGAGTCGGTCGACC
TGATCAAGACGGGCGGCTTCCGAGTGGGGGCCGGTGAGATCGAGACGGTCCTGCTCGGCCACGACGGCGTCGCCGAGGCC
GCGGTGGTCGGGATGCCCGACGACGATCTCGGCCAGCGCATCGTCGCGTTCGTCGTCGGTGATGCGACACCCGAGGCGTT
GATCGATTATGTCGCCGAACAACTCTCGGTGCACAAGCGGCCCCGGGAAGTCCGCATGGTCGACAGCCTGCCCCGCAACG
CGATGGGCAAGGTGCTCAAGAAGGAGCTGACGCAATGGGTTTGA

Upstream 100 bases:

>100_bases
AACGTCAGCCAGTCGTTGCGGTACACCTCGCGCGACGACACCTGCCGGATCGAATCCACGCGACCAGTATTGCCCAGCCC
GGACGAGTAGCCTCGTCGGC

Downstream 100 bases:

>100_bases
GGTTCACCGATCTGTGTGTCGATGCCGCCGACATCCACGCGCTCGGGGGCTGGTGGGCCGAGGTCCTCGGATATGCCCAG
GAAGTCACCGACGACGGTGA

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MLLTSLNPAAVAAGADIADAVTIGEAVLSRSDLVGAATSVAERVGGAQRVAVLATPTPATVLAITGCLIAGVPVVPVPAD
VGVAERRHILTDSGAQAWLGDKPEDTELPHIPVRLHARSWHRYAEPHPDSAALIVYTSGTTGPPKGVVISRRAIAADLDA
LAEAWQWTAEDTLVHGLPLYHVHGLVLGLLGSLRIGNRFVHTGRPTPEAYAQAQGTLYFGVPTVWSRIAADDDAARALAT
ARLLVSGSAPLPVPVFDRLAASTGHEVVERYGSTESLITLSTRADGERRPGWVGLPLAGVETRLVGDDGAPVPHDGETIA
RLEVRGPTLFDGYLNRPDATADVLGVDGWYRTGDVAVVDGVGMHRIVGRESVDLIKTGGFRVGAGEIETVLLGHDGVAEA
AVVGMPDDDLGQRIVAFVVGDATPEALIDYVAEQLSVHKRPREVRMVDSLPRNAMGKVLKKELTQWV

Sequences:

>Translated_467_residues
MLLTSLNPAAVAAGADIADAVTIGEAVLSRSDLVGAATSVAERVGGAQRVAVLATPTPATVLAITGCLIAGVPVVPVPAD
VGVAERRHILTDSGAQAWLGDKPEDTELPHIPVRLHARSWHRYAEPHPDSAALIVYTSGTTGPPKGVVISRRAIAADLDA
LAEAWQWTAEDTLVHGLPLYHVHGLVLGLLGSLRIGNRFVHTGRPTPEAYAQAQGTLYFGVPTVWSRIAADDDAARALAT
ARLLVSGSAPLPVPVFDRLAASTGHEVVERYGSTESLITLSTRADGERRPGWVGLPLAGVETRLVGDDGAPVPHDGETIA
RLEVRGPTLFDGYLNRPDATADVLGVDGWYRTGDVAVVDGVGMHRIVGRESVDLIKTGGFRVGAGEIETVLLGHDGVAEA
AVVGMPDDDLGQRIVAFVVGDATPEALIDYVAEQLSVHKRPREVRMVDSLPRNAMGKVLKKELTQWV
>Mature_467_residues
MLLTSLNPAAVAAGADIADAVTIGEAVLSRSDLVGAATSVAERVGGAQRVAVLATPTPATVLAITGCLIAGVPVVPVPAD
VGVAERRHILTDSGAQAWLGDKPEDTELPHIPVRLHARSWHRYAEPHPDSAALIVYTSGTTGPPKGVVISRRAIAADLDA
LAEAWQWTAEDTLVHGLPLYHVHGLVLGLLGSLRIGNRFVHTGRPTPEAYAQAQGTLYFGVPTVWSRIAADDDAARALAT
ARLLVSGSAPLPVPVFDRLAASTGHEVVERYGSTESLITLSTRADGERRPGWVGLPLAGVETRLVGDDGAPVPHDGETIA
RLEVRGPTLFDGYLNRPDATADVLGVDGWYRTGDVAVVDGVGMHRIVGRESVDLIKTGGFRVGAGEIETVLLGHDGVAEA
AVVGMPDDDLGQRIVAFVVGDATPEALIDYVAEQLSVHKRPREVRMVDSLPRNAMGKVLKKELTQWV

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=379, Percent_Identity=36.6754617414248, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI187761343, Length=379, Percent_Identity=36.6754617414248, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI157311624, Length=469, Percent_Identity=26.6524520255864, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI157311622, Length=469, Percent_Identity=26.6524520255864, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI122937307, Length=373, Percent_Identity=28.4182305630027, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI156151445, Length=350, Percent_Identity=28.5714285714286, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI58082049, Length=469, Percent_Identity=26.4392324093817, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI42544132, Length=426, Percent_Identity=25.3521126760563, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI40807491, Length=345, Percent_Identity=27.8260869565217, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI38505220, Length=428, Percent_Identity=25.2336448598131, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI115511026, Length=263, Percent_Identity=31.5589353612167, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI57165410, Length=351, Percent_Identity=27.3504273504274, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI57165412, Length=351, Percent_Identity=27.0655270655271, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI42794760, Length=348, Percent_Identity=26.7241379310345, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI42794758, Length=348, Percent_Identity=26.7241379310345, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI42794756, Length=348, Percent_Identity=26.7241379310345, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI28416953, Length=377, Percent_Identity=24.1379310344828, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788107, Length=366, Percent_Identity=33.3333333333333, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI145693145, Length=349, Percent_Identity=31.2320916905444, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI1786810, Length=359, Percent_Identity=30.9192200557103, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1789201, Length=365, Percent_Identity=29.5890410958904, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI221142682, Length=457, Percent_Identity=25.164113785558, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790505, Length=376, Percent_Identity=28.1914893617021, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788595, Length=451, Percent_Identity=26.3858093126386, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786801, Length=303, Percent_Identity=29.042904290429, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=477, Percent_Identity=30.6079664570231, Blast_Score=214, Evalue=7e-56,
Organism=Caenorhabditis elegans, GI32563687, Length=465, Percent_Identity=29.6774193548387, Blast_Score=190, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17559526, Length=386, Percent_Identity=30.0518134715026, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17531443, Length=355, Percent_Identity=29.8591549295775, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71994690, Length=367, Percent_Identity=29.700272479564, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71994703, Length=367, Percent_Identity=29.700272479564, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71994694, Length=367, Percent_Identity=29.700272479564, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=381, Percent_Identity=28.6089238845144, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17560308, Length=359, Percent_Identity=30.08356545961, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17538037, Length=413, Percent_Identity=27.3607748184019, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17560140, Length=361, Percent_Identity=27.4238227146814, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17556552, Length=373, Percent_Identity=26.5415549597855, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25141359, Length=436, Percent_Identity=22.9357798165138, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=357, Percent_Identity=25.7703081232493, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71996755, Length=361, Percent_Identity=26.8698060941828, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17510401, Length=193, Percent_Identity=30.0518134715026, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=341, Percent_Identity=30.4985337243402, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320852, Length=406, Percent_Identity=24.6305418719212, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6319264, Length=375, Percent_Identity=25.8666666666667, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323182, Length=378, Percent_Identity=25.9259259259259, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI18859661, Length=356, Percent_Identity=30.0561797752809, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI21355181, Length=351, Percent_Identity=29.9145299145299, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24581924, Length=357, Percent_Identity=28.2913165266106, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI19922652, Length=359, Percent_Identity=29.2479108635098, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI221329755, Length=401, Percent_Identity=22.6932668329177, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24656500, Length=365, Percent_Identity=29.5890410958904, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24653035, Length=346, Percent_Identity=25.7225433526012, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648260, Length=498, Percent_Identity=25.3012048192771, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281366413, Length=394, Percent_Identity=25.3807106598985, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24666501, Length=394, Percent_Identity=25.3807106598985, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24666497, Length=394, Percent_Identity=25.3807106598985, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21356947, Length=349, Percent_Identity=24.6418338108883, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI161076582, Length=353, Percent_Identity=24.929178470255, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 49152; Mature: 49152

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLTSLNPAAVAAGADIADAVTIGEAVLSRSDLVGAATSVAERVGGAQRVAVLATPTPAT
CEECCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHH
VLAITGCLIAGVPVVPVPADVGVAERRHILTDSGAQAWLGDKPEDTELPHIPVRLHARSW
HHHHHHHHHCCCCCCCCCCCCCHHHCCEEECCCCCCCCCCCCCCCCCCCCCEEEEECCHH
HRYAEPHPDSAALIVYTSGTTGPPKGVVISRRAIAADLDALAEAWQWTAEDTLVHGLPLY
HHCCCCCCCCCEEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCHHHCCCHHH
HVHGLVLGLLGSLRIGNRFVHTGRPTPEAYAQAQGTLYFGVPTVWSRIAADDDAARALAT
HHHHHHHHHHHHHHCCCEEEECCCCCHHHHHCCCCEEEECHHHHHHHHHCCCHHHHHHHH
ARLLVSGSAPLPVPVFDRLAASTGHEVVERYGSTESLITLSTRADGERRPGWVGLPLAGV
HHHEEECCCCCCCHHHHHHHHCCCHHHHHHHCCCCEEEEEEECCCCCCCCCEEECCCCCC
ETRLVGDDGAPVPHDGETIARLEVRGPTLFDGYLNRPDATADVLGVDGWYRTGDVAVVDG
EEEEECCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCCCCEEEECCCCEECCCEEEECC
VGMHRIVGRESVDLIKTGGFRVGAGEIETVLLGHDGVAEAAVVGMPDDDLGQRIVAFVVG
CCHHHHHCCCCCCEEECCCEEECCCCEEEEEECCCCCCCEEEECCCCHHHHHHEEEEEEC
DATPEALIDYVAEQLSVHKRPREVRMVDSLPRNAMGKVLKKELTQWV
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLTSLNPAAVAAGADIADAVTIGEAVLSRSDLVGAATSVAERVGGAQRVAVLATPTPAT
CEECCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHH
VLAITGCLIAGVPVVPVPADVGVAERRHILTDSGAQAWLGDKPEDTELPHIPVRLHARSW
HHHHHHHHHCCCCCCCCCCCCCHHHCCEEECCCCCCCCCCCCCCCCCCCCCEEEEECCHH
HRYAEPHPDSAALIVYTSGTTGPPKGVVISRRAIAADLDALAEAWQWTAEDTLVHGLPLY
HHCCCCCCCCCEEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCHHHCCCHHH
HVHGLVLGLLGSLRIGNRFVHTGRPTPEAYAQAQGTLYFGVPTVWSRIAADDDAARALAT
HHHHHHHHHHHHHHCCCEEEECCCCCHHHHHCCCCEEEECHHHHHHHHHCCCHHHHHHHH
ARLLVSGSAPLPVPVFDRLAASTGHEVVERYGSTESLITLSTRADGERRPGWVGLPLAGV
HHHEEECCCCCCCHHHHHHHHCCCHHHHHHHCCCCEEEEEEECCCCCCCCCEEECCCCCC
ETRLVGDDGAPVPHDGETIARLEVRGPTLFDGYLNRPDATADVLGVDGWYRTGDVAVVDG
EEEEECCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCCCCEEEECCCCEECCCEEEECC
VGMHRIVGRESVDLIKTGGFRVGAGEIETVLLGHDGVAEAAVVGMPDDDLGQRIVAFVVG
CCHHHHHCCCCCCEEECCCEEECCCCEEEEEECCCCCCCEEEECCCCHHHHHHEEEEEEC
DATPEALIDYVAEQLSVHKRPREVRMVDSLPRNAMGKVLKKELTQWV
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]