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The map label for this gene is folP2 [H]

Identifier: 108800970

GI number: 108800970

Start: 4270235

End: 4271110

Strand: Reverse

Name: folP2 [H]

Synonym: Mmcs_4006

Alternate gene names: 108800970

Gene position: 4271110-4270235 (Counterclockwise)

Preceding gene: 108800971

Following gene: 108800969

Centisome position: 74.86

GC content: 68.84

Gene sequence:

>876_bases
GTGGAGTCGACCTTCTGCGGACGTCCGGTCGCCGGTGACCGCGCCATGATCATGGCGATCGTGAACCGGACGCCGGACTC
GTTCTACGACCGGGGCGCGACGTTCACCGACGAGGCCGCCAAATCCGCCGCCTACCGCGTCGTCGAGGACGGGGCCGACG
TGGTCGACGTCGGCGGGGTCAAGGCCGGTCCGGGTCAGACCGTGGGCGCCGACGAGGAGATCGCCCGCGTCGTGCCGTTC
ATCGAATGGCTGCGCGGCACGTTCCCCGACGTGGTGATCAGCGTCGACACCTGGCGGGCCTCGGTGGCCAAACAGGCCTG
CGCCGCCGGCGCCGATCTGATCAACGACACCTGGGGCGGTAGCGACCCCGGACTGCCCGAGGTCGCCGCCGAGTTCAACG
CCGGGCTGGTCTGCTCCCACACCGGGGGGGCGGTGCCACGCACCCGCCCGTTCCGCGTCAACTACGGCATCACCGAACGC
GGCGTGGTCGACGACGTCATCGCCGAGGTCACCTCGGCGGCGCAGCGGGCGGTCGCCGCGGGCGTCGCGCGGGACCGGAT
TCTGATCGATCCGACGCACGATTTCGGCAAGAACACTTACCACGGTCTTAGTTTGTTGCGGCACGTGGAAGAGCTTGTAA
AAACTGGATGGCCGGTCCTGATGGCGCTGAGCAACAAGGATTTCGTCGGGGAGACTCTGGGTGTGGGTTTGACTGAACGC
CTCGAGGGCACCCTCGCAGCAACCGCGCTGGCAGCGCACGCCGGTGCGGCGATGTTCCGGGTGCACGAGGTCGGGCCCAC
TCGGCGCGTACTGGAGATGGTCGCGTCGATCCAAGGCGTGCGTCCGCCGACGCGCACGGTGAGGGGACTGGCATGA

Upstream 100 bases:

>100_bases
CAAGGAAGGCAAGAAGCCGAAGTAGCGGCGATTCGGGCGTGGTTGGTCGCGCCCAGCGCGACCGAACACGCCGAAATCAC
CCCGCCCGTAGCCTGGCAGG

Downstream 100 bases:

>100_bases
CTGTTATTTCGCGGTTACCGGAACTGGAGACACCAGAACTGCACTCCGCCGACGCGATCGCGCGGCACCGCTGGTTCGAC
ACCCACAGTTGGAACCGTCC

Product: dihydropteroate synthase

Products: diphosphate; dihydropteroate

Alternate protein names: DHPS 2; Dihydropteroate pyrophosphorylase 2 [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MESTFCGRPVAGDRAMIMAIVNRTPDSFYDRGATFTDEAAKSAAYRVVEDGADVVDVGGVKAGPGQTVGADEEIARVVPF
IEWLRGTFPDVVISVDTWRASVAKQACAAGADLINDTWGGSDPGLPEVAAEFNAGLVCSHTGGAVPRTRPFRVNYGITER
GVVDDVIAEVTSAAQRAVAAGVARDRILIDPTHDFGKNTYHGLSLLRHVEELVKTGWPVLMALSNKDFVGETLGVGLTER
LEGTLAATALAAHAGAAMFRVHEVGPTRRVLEMVASIQGVRPPTRTVRGLA

Sequences:

>Translated_291_residues
MESTFCGRPVAGDRAMIMAIVNRTPDSFYDRGATFTDEAAKSAAYRVVEDGADVVDVGGVKAGPGQTVGADEEIARVVPF
IEWLRGTFPDVVISVDTWRASVAKQACAAGADLINDTWGGSDPGLPEVAAEFNAGLVCSHTGGAVPRTRPFRVNYGITER
GVVDDVIAEVTSAAQRAVAAGVARDRILIDPTHDFGKNTYHGLSLLRHVEELVKTGWPVLMALSNKDFVGETLGVGLTER
LEGTLAATALAAHAGAAMFRVHEVGPTRRVLEMVASIQGVRPPTRTVRGLA
>Mature_291_residues
MESTFCGRPVAGDRAMIMAIVNRTPDSFYDRGATFTDEAAKSAAYRVVEDGADVVDVGGVKAGPGQTVGADEEIARVVPF
IEWLRGTFPDVVISVDTWRASVAKQACAAGADLINDTWGGSDPGLPEVAAEFNAGLVCSHTGGAVPRTRPFRVNYGITER
GVVDDVIAEVTSAAQRAVAAGVARDRILIDPTHDFGKNTYHGLSLLRHVEELVKTGWPVLMALSNKDFVGETLGVGLTER
LEGTLAATALAAHAGAAMFRVHEVGPTRRVLEMVASIQGVRPPTRTVRGLA

Specific function: Has very low affinity for the DHPS substrate 6- hydroxymethyl-7,8-dihydropterin-pyrophosphate, but can bind the inhibitor dapsone. Seems to lack dihydropteroate synthase activity, and does probably not function in folate metabolism [H]

COG id: COG0294

COG function: function code H; Dihydropteroate synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082224, Length=274, Percent_Identity=36.1313868613139, Blast_Score=153, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324073, Length=314, Percent_Identity=29.6178343949045, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006390
- InterPro:   IPR011005
- InterPro:   IPR000489 [H]

Pfam domain/function: PF00809 Pterin_bind [H]

EC number: 2.5.1.15

Molecular weight: Translated: 30751; Mature: 30751

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50972 PTERIN_BINDING ; PS00792 DHPS_1 ; PS00793 DHPS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESTFCGRPVAGDRAMIMAIVNRTPDSFYDRGATFTDEAAKSAAYRVVEDGADVVDVGGV
CCCCCCCCCCCCCHHEEHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEECCCC
KAGPGQTVGADEEIARVVPFIEWLRGTFPDVVISVDTWRASVAKQACAAGADLINDTWGG
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCC
SDPGLPEVAAEFNAGLVCSHTGGAVPRTRPFRVNYGITERGVVDDVIAEVTSAAQRAVAA
CCCCCHHHHHHCCCCEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
GVARDRILIDPTHDFGKNTYHGLSLLRHVEELVKTGWPVLMALSNKDFVGETLGVGLTER
HHHCCCEEECCCHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCHHHH
LEGTLAATALAAHAGAAMFRVHEVGPTRRVLEMVASIQGVRPPTRTVRGLA
HHHHHHHHHHHHHHCHHHEEEHHCCHHHHHHHHHHHHCCCCCHHHHHCCCC
>Mature Secondary Structure
MESTFCGRPVAGDRAMIMAIVNRTPDSFYDRGATFTDEAAKSAAYRVVEDGADVVDVGGV
CCCCCCCCCCCCCHHEEHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEECCCC
KAGPGQTVGADEEIARVVPFIEWLRGTFPDVVISVDTWRASVAKQACAAGADLINDTWGG
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCC
SDPGLPEVAAEFNAGLVCSHTGGAVPRTRPFRVNYGITERGVVDDVIAEVTSAAQRAVAA
CCCCCHHHHHHCCCCEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
GVARDRILIDPTHDFGKNTYHGLSLLRHVEELVKTGWPVLMALSNKDFVGETLGVGLTER
HHHCCCEEECCCHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCHHHH
LEGTLAATALAAHAGAAMFRVHEVGPTRRVLEMVASIQGVRPPTRTVRGLA
HHHHHHHHHHHHHHCHHHEEEHHCCHHHHHHHHHHHHCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0015 {4-aminobenzoate}} [C]

Substrates: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate; 4-aminobenzoate

Specific reaction: 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate

General reaction: Aryl group transfer [C]

Inhibitor: Sulfaguanidine; Sulfanilamide; Sulfathiazole; Sulfonamides [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]