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The map label for this gene is cysNC [H]

Identifier: 108800882

GI number: 108800882

Start: 4171235

End: 4173094

Strand: Reverse

Name: cysNC [H]

Synonym: Mmcs_3918

Alternate gene names: 108800882

Gene position: 4173094-4171235 (Counterclockwise)

Preceding gene: 108800883

Following gene: 108800881

Centisome position: 73.14

GC content: 67.8

Gene sequence:

>1860_bases
ATGACGCAAACCACCACGCTGCTCCGCATCGCCACCGCCGGGTCCGTCGACGACGGTAAGTCGACGTTGATCGGCCGGCT
GCTCTACGACTCCAAGGCCGTCATGGAGGATCAGCTCGCCGCCGTCGAGCGCACCTCCAAGGAACGCGGTAACGACTACA
CCGACCTCGCCCTGGTGACCGACGGTCTGCGCTCCGAGCGGGAACAGGGCATCACGATCGACGTGGCCTACCGCTACTTC
GCCACGGCCAAGCGGAAATTCATCATCGCCGACACCCCCGGCCACCTGCAGTACACGCGCAACATGGTGACCGGCACGTC
GACCGCGCAGCTGGCGATCGTCCTCGTCGACGCCCGCCACGGGCTGCTCGAACAGTCGCGTAGGCATGCGTTCCTGGCGT
CGCTGCTGGGCGTCCAGCACATCGTGCTCGCGGTCAACAAGATGGACCTGATCGACTGGAATCAGGAGAAGTTCAACGAC
ATCCGCGACGAGTTCCACGCCTTCGCCGCACGTCTGGACATCCACGACGTCACCACCATCCCGATGTCGGCGCTGCTCGG
GGACAACGTCGTCACCAAATCGGACAAGGCCCCGTGGTACGACGGGCCCGCGCTGCTGAGCCACCTCGAAGAGGTCTACA
TCGCCGGCGACCGCAACCTCACCGACGTCCGCTTCCCGGTGCAGTACGTCATCCGCCCGCAGACCCGCGACCACGCCGAC
CACCGCAGCTACGCCGGCACCGTTGCGAGCGGCGTGATGCGCGTCGGCGACGAGATCGTCGTGCTGCCCAGCGGTAAGAC
CAGCATTATCACCGCGATCGACTCGCCGACCGGTCCGGTCGGCGAGGCGTTCCCGCCGATGGCGGTGTCGGTCAGCCTGG
CCGACGATATCGACATCAGCCGCGGCGACGTGATCGCCCGCGTCCACAACCAGCCGCACGTGTCCAACGAGTTCGACGCG
ACCGTCTGCTGGATGGCCGACGCCTCGGCGCTCGAACCCGGCCGCGAATACCTGATCAAGCACACCACCCGGGTCACCCG
CGCCCGCGTCGCGTCGCTCGATTACCGCCTCGACGTCAACACCCTGCACCGCGACAAATCGGCCACCGCGCTCAAGCTCA
ACGAACTCGGCCGGGTCACGCTGCGCACCCAGCAGCCGCTGCTGCTCGACGAGTACTCCCGCAACGCCGCCACGGGCTCG
TTCATCCTGATCGACCCGGACACCAACGGCACGGTCGCCGCGGGCATGGTCCGCGACACCACCCCGGTGGCCGCGCGCAC
CGCCACCCCGAACACCGTGCGCCATCAGTCGCTGGTCAGCGCCAAGGACCGGCTCTCCAGGGGCCGCACCGTGTGGTTCA
CCGGGCTTTCGGGCTCCGGCAAATCCTCGGTGGCGGTGCGGGTGGAGCAGAAGCTCCTCGAACGGGGTTGTCCCGCTTAT
GTTCTCGACGGTGACAATCTGCGCCACGGCCTCAACGCCGACCTCGGCTTCTCGATGGCCGACCGGGCGGAGAACCTGCG
GCGGCTCGCCCACATCGCGACGCTGATGGCCGATTCCGGCCTGACCGTGCTGGTTCCTGCGATCAGCCCGCTCGCCGAGC
ATCGCGAACTCGCGCGCGCGGTACACGTCGACCAGGGCTACGACTTCTTCGAGGTGTTCTGCGACACCCCGCTGGAGGAC
TGCGAACGGCGCGACCCCAAGGGCCTCTACGCCAAGGCGCGCGCCGGTGAGATCACCCACTTCACCGGGATCGACAGCCC
CTACCAGCGCCCGAAGAACCCGGACCTGCGGCTGACCCCGGACCGCGACGTCGACGACCTGGCCCAGCTGGTCATCGACA
TGCTGGACGGCAGCCGGTGA

Upstream 100 bases:

>100_bases
AGACCGCGGTGTCGCGGCTGACCGAGCGCGGTGCGACGCGCGCCGACGACCGCATCTCCGAGGCCGGGATGGAAGACCGT
AAGCGCGAGGGGTACTTCTG

Downstream 100 bases:

>100_bases
ACGATCACGAGCTGGCCGCCCGGCTCGCCACCCGGGCGGGCGACCTGCTGCTCGACGTACGTGCGGAGTTCGCCGACACC
TCGGCCGAGGAGCGGAAAGC

Product: bifunctional sulfate adenylyltransferase subunit 1/adenylylsulfate kinase protein

Products: NA

Alternate protein names: Sulfate adenylyltransferase subunit 1; ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; APS kinase; ATP adenosine-5'-phosphosulfate 3'-phosphotransferase [H]

Number of amino acids: Translated: 619; Mature: 618

Protein sequence:

>619_residues
MTQTTTLLRIATAGSVDDGKSTLIGRLLYDSKAVMEDQLAAVERTSKERGNDYTDLALVTDGLRSEREQGITIDVAYRYF
ATAKRKFIIADTPGHLQYTRNMVTGTSTAQLAIVLVDARHGLLEQSRRHAFLASLLGVQHIVLAVNKMDLIDWNQEKFND
IRDEFHAFAARLDIHDVTTIPMSALLGDNVVTKSDKAPWYDGPALLSHLEEVYIAGDRNLTDVRFPVQYVIRPQTRDHAD
HRSYAGTVASGVMRVGDEIVVLPSGKTSIITAIDSPTGPVGEAFPPMAVSVSLADDIDISRGDVIARVHNQPHVSNEFDA
TVCWMADASALEPGREYLIKHTTRVTRARVASLDYRLDVNTLHRDKSATALKLNELGRVTLRTQQPLLLDEYSRNAATGS
FILIDPDTNGTVAAGMVRDTTPVAARTATPNTVRHQSLVSAKDRLSRGRTVWFTGLSGSGKSSVAVRVEQKLLERGCPAY
VLDGDNLRHGLNADLGFSMADRAENLRRLAHIATLMADSGLTVLVPAISPLAEHRELARAVHVDQGYDFFEVFCDTPLED
CERRDPKGLYAKARAGEITHFTGIDSPYQRPKNPDLRLTPDRDVDDLAQLVIDMLDGSR

Sequences:

>Translated_619_residues
MTQTTTLLRIATAGSVDDGKSTLIGRLLYDSKAVMEDQLAAVERTSKERGNDYTDLALVTDGLRSEREQGITIDVAYRYF
ATAKRKFIIADTPGHLQYTRNMVTGTSTAQLAIVLVDARHGLLEQSRRHAFLASLLGVQHIVLAVNKMDLIDWNQEKFND
IRDEFHAFAARLDIHDVTTIPMSALLGDNVVTKSDKAPWYDGPALLSHLEEVYIAGDRNLTDVRFPVQYVIRPQTRDHAD
HRSYAGTVASGVMRVGDEIVVLPSGKTSIITAIDSPTGPVGEAFPPMAVSVSLADDIDISRGDVIARVHNQPHVSNEFDA
TVCWMADASALEPGREYLIKHTTRVTRARVASLDYRLDVNTLHRDKSATALKLNELGRVTLRTQQPLLLDEYSRNAATGS
FILIDPDTNGTVAAGMVRDTTPVAARTATPNTVRHQSLVSAKDRLSRGRTVWFTGLSGSGKSSVAVRVEQKLLERGCPAY
VLDGDNLRHGLNADLGFSMADRAENLRRLAHIATLMADSGLTVLVPAISPLAEHRELARAVHVDQGYDFFEVFCDTPLED
CERRDPKGLYAKARAGEITHFTGIDSPYQRPKNPDLRLTPDRDVDDLAQLVIDMLDGSR
>Mature_618_residues
TQTTTLLRIATAGSVDDGKSTLIGRLLYDSKAVMEDQLAAVERTSKERGNDYTDLALVTDGLRSEREQGITIDVAYRYFA
TAKRKFIIADTPGHLQYTRNMVTGTSTAQLAIVLVDARHGLLEQSRRHAFLASLLGVQHIVLAVNKMDLIDWNQEKFNDI
RDEFHAFAARLDIHDVTTIPMSALLGDNVVTKSDKAPWYDGPALLSHLEEVYIAGDRNLTDVRFPVQYVIRPQTRDHADH
RSYAGTVASGVMRVGDEIVVLPSGKTSIITAIDSPTGPVGEAFPPMAVSVSLADDIDISRGDVIARVHNQPHVSNEFDAT
VCWMADASALEPGREYLIKHTTRVTRARVASLDYRLDVNTLHRDKSATALKLNELGRVTLRTQQPLLLDEYSRNAATGSF
ILIDPDTNGTVAAGMVRDTTPVAARTATPNTVRHQSLVSAKDRLSRGRTVWFTGLSGSGKSSVAVRVEQKLLERGCPAYV
LDGDNLRHGLNADLGFSMADRAENLRRLAHIATLMADSGLTVLVPAISPLAEHRELARAVHVDQGYDFFEVFCDTPLEDC
ERRDPKGLYAKARAGEITHFTGIDSPYQRPKNPDLRLTPDRDVDDLAQLVIDMLDGSR

Specific function: APS kinase catalyzes the synthesis of activated sulfate [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the APS kinase family [H]

Homologues:

Organism=Homo sapiens, GI46094058, Length=170, Percent_Identity=54.1176470588235, Blast_Score=190, Evalue=4e-48,
Organism=Homo sapiens, GI34447231, Length=170, Percent_Identity=51.7647058823529, Blast_Score=185, Evalue=1e-46,
Organism=Homo sapiens, GI62912492, Length=170, Percent_Identity=51.7647058823529, Blast_Score=185, Evalue=1e-46,
Organism=Homo sapiens, GI194018522, Length=432, Percent_Identity=27.0833333333333, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI223555963, Length=428, Percent_Identity=29.2056074766355, Blast_Score=166, Evalue=7e-41,
Organism=Homo sapiens, GI5729864, Length=428, Percent_Identity=29.2056074766355, Blast_Score=166, Evalue=7e-41,
Organism=Homo sapiens, GI194097354, Length=432, Percent_Identity=27.0833333333333, Blast_Score=166, Evalue=7e-41,
Organism=Homo sapiens, GI194018520, Length=432, Percent_Identity=27.0833333333333, Blast_Score=166, Evalue=8e-41,
Organism=Homo sapiens, GI4503475, Length=446, Percent_Identity=26.6816143497758, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI46094014, Length=432, Percent_Identity=26.1574074074074, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI4503471, Length=445, Percent_Identity=26.7415730337079, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI34147630, Length=422, Percent_Identity=24.8815165876777, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI1789108, Length=432, Percent_Identity=50, Blast_Score=401, Evalue=1e-113,
Organism=Escherichia coli, GI1789107, Length=189, Percent_Identity=50.7936507936508, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1790412, Length=148, Percent_Identity=29.0540540540541, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789737, Length=148, Percent_Identity=29.0540540540541, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17542422, Length=171, Percent_Identity=51.4619883040936, Blast_Score=177, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI32566629, Length=432, Percent_Identity=28.2407407407407, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17552884, Length=445, Percent_Identity=27.8651685393258, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=445, Percent_Identity=27.8651685393258, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI115532067, Length=266, Percent_Identity=30.8270676691729, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI115532065, Length=266, Percent_Identity=30.8270676691729, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI32566303, Length=438, Percent_Identity=25.7990867579909, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=38.9261744966443, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17556456, Length=421, Percent_Identity=25.6532066508314, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI25141371, Length=283, Percent_Identity=25.4416961130742, Blast_Score=84, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322852, Length=173, Percent_Identity=49.7109826589595, Blast_Score=173, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6325337, Length=447, Percent_Identity=27.0693512304251, Blast_Score=163, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=447, Percent_Identity=27.0693512304251, Blast_Score=163, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=400, Percent_Identity=27.25, Blast_Score=160, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6320377, Length=425, Percent_Identity=28, Blast_Score=133, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6324761, Length=146, Percent_Identity=39.7260273972603, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24667032, Length=150, Percent_Identity=56.6666666666667, Blast_Score=186, Evalue=6e-47,
Organism=Drosophila melanogaster, GI24667028, Length=150, Percent_Identity=56.6666666666667, Blast_Score=186, Evalue=6e-47,
Organism=Drosophila melanogaster, GI24667036, Length=150, Percent_Identity=56.6666666666667, Blast_Score=186, Evalue=6e-47,
Organism=Drosophila melanogaster, GI116007838, Length=150, Percent_Identity=56.6666666666667, Blast_Score=185, Evalue=8e-47,
Organism=Drosophila melanogaster, GI24667044, Length=150, Percent_Identity=56.6666666666667, Blast_Score=185, Evalue=8e-47,
Organism=Drosophila melanogaster, GI24667040, Length=150, Percent_Identity=56.6666666666667, Blast_Score=185, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24652838, Length=445, Percent_Identity=26.5168539325843, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI17137572, Length=445, Percent_Identity=26.5168539325843, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI17137380, Length=430, Percent_Identity=27.4418604651163, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI45553807, Length=445, Percent_Identity=25.6179775280899, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI45553816, Length=445, Percent_Identity=25.6179775280899, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24651721, Length=445, Percent_Identity=25.6179775280899, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17864154, Length=445, Percent_Identity=25.6179775280899, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI45550900, Length=428, Percent_Identity=28.7383177570093, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI281363316, Length=203, Percent_Identity=32.0197044334975, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17864358, Length=203, Percent_Identity=32.0197044334975, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19921738, Length=180, Percent_Identity=31.6666666666667, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002891
- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF01583 APS_kinase; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4; =2.7.1.25 [H]

Molecular weight: Translated: 68140; Mature: 68009

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTTTLLRIATAGSVDDGKSTLIGRLLYDSKAVMEDQLAAVERTSKERGNDYTDLALVT
CCCCEEEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEHHEEH
DGLRSEREQGITIDVAYRYFATAKRKFIIADTPGHLQYTRNMVTGTSTAQLAIVLVDARH
HHHHHHHHCCCEEEEEHHHHHHCCCEEEEECCCCCHHHHHHEECCCCCCEEEEEEEECCC
GLLEQSRRHAFLASLLGVQHIVLAVNKMDLIDWNQEKFNDIRDEFHAFAARLDIHDVTTI
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHH
PMSALLGDNVVTKSDKAPWYDGPALLSHLEEVYIAGDRNLTDVRFPVQYVIRPQTRDHAD
HHHHHHCCCCEECCCCCCCCCCHHHHHHHHHEEEECCCCCEEEECCEEEEECCCCCCCCC
HRSYAGTVASGVMRVGDEIVVLPSGKTSIITAIDSPTGPVGEAFPPMAVSVSLADDIDIS
HHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCC
RGDVIARVHNQPHVSNEFDATVCWMADASALEPGREYLIKHTTRVTRARVASLDYRLDVN
CCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEHH
TLHRDKSATALKLNELGRVTLRTQQPLLLDEYSRNAATGSFILIDPDTNGTVAAGMVRDT
HHCCCCCCCEEEECCCCEEEEECCCCCHHHHHCCCCCCCEEEEECCCCCCCEEECEEECC
TPVAARTATPNTVRHQSLVSAKDRLSRGRTVWFTGLSGSGKSSVAVRVEQKLLERGCPAY
CCCEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHHCCCCEE
VLDGDNLRHGLNADLGFSMADRAENLRRLAHIATLMADSGLTVLVPAISPLAEHRELARA
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHH
VHVDQGYDFFEVFCDTPLEDCERRDPKGLYAKARAGEITHFTGIDSPYQRPKNPDLRLTP
HHCCCCCCHHHHHHCCCHHHHHCCCCCCCEEECCCCCEEEECCCCCHHCCCCCCCEEECC
DRDVDDLAQLVIDMLDGSR
CCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQTTTLLRIATAGSVDDGKSTLIGRLLYDSKAVMEDQLAAVERTSKERGNDYTDLALVT
CCCEEEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEHHEEH
DGLRSEREQGITIDVAYRYFATAKRKFIIADTPGHLQYTRNMVTGTSTAQLAIVLVDARH
HHHHHHHHCCCEEEEEHHHHHHCCCEEEEECCCCCHHHHHHEECCCCCCEEEEEEEECCC
GLLEQSRRHAFLASLLGVQHIVLAVNKMDLIDWNQEKFNDIRDEFHAFAARLDIHDVTTI
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHH
PMSALLGDNVVTKSDKAPWYDGPALLSHLEEVYIAGDRNLTDVRFPVQYVIRPQTRDHAD
HHHHHHCCCCEECCCCCCCCCCHHHHHHHHHEEEECCCCCEEEECCEEEEECCCCCCCCC
HRSYAGTVASGVMRVGDEIVVLPSGKTSIITAIDSPTGPVGEAFPPMAVSVSLADDIDIS
HHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCC
RGDVIARVHNQPHVSNEFDATVCWMADASALEPGREYLIKHTTRVTRARVASLDYRLDVN
CCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEHH
TLHRDKSATALKLNELGRVTLRTQQPLLLDEYSRNAATGSFILIDPDTNGTVAAGMVRDT
HHCCCCCCCEEEECCCCEEEEECCCCCHHHHHCCCCCCCEEEEECCCCCCCEEECEEECC
TPVAARTATPNTVRHQSLVSAKDRLSRGRTVWFTGLSGSGKSSVAVRVEQKLLERGCPAY
CCCEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHHCCCCEE
VLDGDNLRHGLNADLGFSMADRAENLRRLAHIATLMADSGLTVLVPAISPLAEHRELARA
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHH
VHVDQGYDFFEVFCDTPLEDCERRDPKGLYAKARAGEITHFTGIDSPYQRPKNPDLRLTP
HHCCCCCCHHHHHHCCCHHHHHCCCCCCCEEECCCCCEEEECCCCCHHCCCCCCCEEECC
DRDVDDLAQLVIDMLDGSR
CCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]