Click here to switch to the map view.

The map label for this gene is cysD [H]

Identifier: 108800883

GI number: 108800883

Start: 4173094

End: 4174023

Strand: Reverse

Name: cysD [H]

Synonym: Mmcs_3919

Alternate gene names: 108800883

Gene position: 4174023-4173094 (Counterclockwise)

Preceding gene: 108800885

Following gene: 108800882

Centisome position: 73.16

GC content: 67.96

Gene sequence:

>930_bases
ATGACAGCCGCTCATGTAGCCGCTCCGGAGCCGGGCCAATACGAGCTGAGCCATCTGCGCCTGCTCGAGGCCGAAGCCAT
CCACATCATCCGCGAGGTCGCCGCCGAATTCGAGCGTCCGGTGCTGCTGTTCTCCGGCGGCAAGGACTCGATTGTCATGC
TGCATCTGGCGATCAAGGCCTTCGCGCCGGCCCGTCTGCCGTTCCCGGTCATGCACGTCGACACCGGCCACAACTTCGAC
GAGGTCATCTCCACGCGCGACCGCCTGGTCGCCGAAAACGGCGTCCGCCTGGTCGTCGCCTCGGTGCAGGAGGACATCGA
CGCGGGCCGCGTGGTCGACAACGGGCCCTCGCGCAACCCGCTGCAGACCATCACGCTGTTGCGCGCCATCCGCGAGAACC
GCTTCGACGCCGCGTTCGGCGGCGCTCGCCGCGACGAGGAGAAGGCCCGCGCCAAGGAGCGGGTGTTCAGCTTCCGCGAC
GAGTTCGGCCAGTGGGATCCGAAGGCGCAGCGGCCGGAGCTGTGGAACATCTACAACGGCAGGCACCGCAAGGGTGAGCA
CATCCGGGTGTTCCCGCTGTCCAACTGGACCGAGTACGACATCTGGGCCTACATCGGCGCCGAGGGCATCACGCTGCCGG
GGATCTACTACGCGCACACCCGTCCGGTGTTCCAGCGCGACGGGATGCTGCTGGCCGTGCACCCGTACATGCAGCCGCGC
GACGACGAAGAGGTCTTCGAGACCAGCGTGCGGTTCCGCACCGTCGGCGACGTCACCTGCACCGGGTGCGTCGAATCGAC
CGCGTCGACGGTGGAGGACATCATCGCCGAGACCGCGGTGTCGCGGCTGACCGAGCGCGGTGCGACGCGCGCCGACGACC
GCATCTCCGAGGCCGGGATGGAAGACCGTAAGCGCGAGGGGTACTTCTGA

Upstream 100 bases:

>100_bases
GCGGGCGCTCGGGCCGCCTTGACACCGCCATCGTCTCGCCGGTAATTTTTCGGTAATCCGCGTAAATCTGGCAAGATCAA
CCAACTTAACGAGGAATCCG

Downstream 100 bases:

>100_bases
TGACGCAAACCACCACGCTGCTCCGCATCGCCACCGCCGGGTCCGTCGACGACGGTAAGTCGACGTTGATCGGCCGGCTG
CTCTACGACTCCAAGGCCGT

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MTAAHVAAPEPGQYELSHLRLLEAEAIHIIREVAAEFERPVLLFSGGKDSIVMLHLAIKAFAPARLPFPVMHVDTGHNFD
EVISTRDRLVAENGVRLVVASVQEDIDAGRVVDNGPSRNPLQTITLLRAIRENRFDAAFGGARRDEEKARAKERVFSFRD
EFGQWDPKAQRPELWNIYNGRHRKGEHIRVFPLSNWTEYDIWAYIGAEGITLPGIYYAHTRPVFQRDGMLLAVHPYMQPR
DDEEVFETSVRFRTVGDVTCTGCVESTASTVEDIIAETAVSRLTERGATRADDRISEAGMEDRKREGYF

Sequences:

>Translated_309_residues
MTAAHVAAPEPGQYELSHLRLLEAEAIHIIREVAAEFERPVLLFSGGKDSIVMLHLAIKAFAPARLPFPVMHVDTGHNFD
EVISTRDRLVAENGVRLVVASVQEDIDAGRVVDNGPSRNPLQTITLLRAIRENRFDAAFGGARRDEEKARAKERVFSFRD
EFGQWDPKAQRPELWNIYNGRHRKGEHIRVFPLSNWTEYDIWAYIGAEGITLPGIYYAHTRPVFQRDGMLLAVHPYMQPR
DDEEVFETSVRFRTVGDVTCTGCVESTASTVEDIIAETAVSRLTERGATRADDRISEAGMEDRKREGYF
>Mature_308_residues
TAAHVAAPEPGQYELSHLRLLEAEAIHIIREVAAEFERPVLLFSGGKDSIVMLHLAIKAFAPARLPFPVMHVDTGHNFDE
VISTRDRLVAENGVRLVVASVQEDIDAGRVVDNGPSRNPLQTITLLRAIRENRFDAAFGGARRDEEKARAKERVFSFRDE
FGQWDPKAQRPELWNIYNGRHRKGEHIRVFPLSNWTEYDIWAYIGAEGITLPGIYYAHTRPVFQRDGMLLAVHPYMQPRD
DEEVFETSVRFRTVGDVTCTGCVESTASTVEDIIAETAVSRLTERGATRADDRISEAGMEDRKREGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=303, Percent_Identity=53.4653465346535, Blast_Score=295, Evalue=3e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 34951; Mature: 34820

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAHVAAPEPGQYELSHLRLLEAEAIHIIREVAAEFERPVLLFSGGKDSIVMLHLAIKA
CCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEHHHH
FAPARLPFPVMHVDTGHNFDEVISTRDRLVAENGVRLVVASVQEDIDAGRVVDNGPSRNP
HCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCCEECCCCCCCH
LQTITLLRAIRENRFDAAFGGARRDEEKARAKERVFSFRDEFGQWDPKAQRPELWNIYNG
HHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
RHRKGEHIRVFPLSNWTEYDIWAYIGAEGITLPGIYYAHTRPVFQRDGMLLAVHPYMQPR
CCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCCEEEECCCCHHHHCCEEEEEECCCCCC
DDEEVFETSVRFRTVGDVTCTGCVESTASTVEDIIAETAVSRLTERGATRADDRISEAGM
CHHHHHHHHHHEEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCC
EDRKREGYF
HHHHHCCCC
>Mature Secondary Structure 
TAAHVAAPEPGQYELSHLRLLEAEAIHIIREVAAEFERPVLLFSGGKDSIVMLHLAIKA
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEHHHH
FAPARLPFPVMHVDTGHNFDEVISTRDRLVAENGVRLVVASVQEDIDAGRVVDNGPSRNP
HCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEHHHCCCCCCEECCCCCCCH
LQTITLLRAIRENRFDAAFGGARRDEEKARAKERVFSFRDEFGQWDPKAQRPELWNIYNG
HHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
RHRKGEHIRVFPLSNWTEYDIWAYIGAEGITLPGIYYAHTRPVFQRDGMLLAVHPYMQPR
CCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCCEEEECCCCHHHHCCEEEEEECCCCCC
DDEEVFETSVRFRTVGDVTCTGCVESTASTVEDIIAETAVSRLTERGATRADDRISEAGM
CHHHHHHHHHHEEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCC
EDRKREGYF
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA