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The map label for this gene is proP [H]

Identifier: 108800877

GI number: 108800877

Start: 4166354

End: 4167736

Strand: Reverse

Name: proP [H]

Synonym: Mmcs_3913

Alternate gene names: 108800877

Gene position: 4167736-4166354 (Counterclockwise)

Preceding gene: 108800878

Following gene: 108800871

Centisome position: 73.05

GC content: 66.67

Gene sequence:

>1383_bases
GTGGATTCCGAACATGATCGGCAAGATGATCGACCGACCACCGCCGACGGCCACCCCGACCCTGGAGTCGTGAAGAAGGC
GATCGCCGCGTCCGCGATCGGCAACGCGACCGAGTGGTTCGACTACGGCATCTACGCCTACGGCGTCAGCTACATCTCCG
CGGCGATCTTCCCCGGGGAGGCCGGGCGCGCGACGCTGCTCGCGCTGATGACGTTCGCGGTGTCGTTCTTGGTCAGACCG
CTGGGTGGCTTCGTCTGGGGCCCGCTGGGCGACCGCCTCGGCCGCAAGCGGGTGCTCGCGATCACGATCGTGCTGATGGC
CGGCGCGACCTTCTGCGTGGGGCTGGTGCCCACATACGCCGCCATCGGGATGTGGGCGCCCTTCCTGCTGGTCCTCCTGC
GGATGATCCAGGGCTTCTCCACCGGAGGCGAGTACGGCGGCGCCGCCACGTTCATGGCCGAATACGCCCCGAACCGCCGC
CGTGGACTGCTCGGCAGCTTCCTCGAATTCGGGACCCTCGGCGGGTTCGCGATCGGTGCGTTGCTCATGCTGGGCTGCTC
TCTGGTGCTCGGCGACGACCAGATGCACGACTGGGGTTGGCGGCTGCCGTTCCTGCTCGCCGCCCCACTCGGACTCGTCG
GCGTCTATTTGCGGTCGCGGCTCGAAGACACCCCGGTCTTCCGTGAACTCGAGGAGAAGGGTGAGGCCGAGCCTCAGGTG
ACCGCGGCGTTCCGCGATCTGCTCGCCCGCTACTGGCGGCCGATCCTGAAGCTCGGCGGCATGGTCGTGGCGCTGAACGT
CGTCAACTACACGCTGCTCAGCTACATGCCGACCTATCTGGAGACCCAGATCGGGCTGTCGGCGGACCAGTCGCTGCTCG
TACCGATCATCGGCATGCTGGCGATGATGGTGTTCGTGCCGTTCGTCGGCCTGCTGAGCGATCGGGTGGGGCGCAAACCC
CTGTGGTGGTTCTCGCTGATCGGCCTCTTCGTGGCCGGTGTGCCGATGTTCCTGCTGATGGGCACCAACCTGGCCGGGGC
GGTGATCGGGTTCGCGGTGTTGGGGCTGCTCTACGTACCGCAGCTGGCGACCATTTCGGCCACCTTCCCGGCCATGTTCC
CCACCCAGGTGCGCTACGCCGGATTCGCGATCGCCTACAACGTGTCGACCTCACTCTTCGGCGGCACCGCACCGGCGGTC
AACGAATGGCTCACCAACACCACCGGCGATGTGCTGTTCCCGGCGTACTACATGATGGCCGCGTGTGTGATCGGTGCGCT
GGCGCTGATCAAGGTCCCGGAGACCGCGCGCTGTCCGATCAACGGCACCGTGACGCCCGGCACAGATGAGGCACCTGATC
CGGTTCCCTACGAGAAGGTCTGA

Upstream 100 bases:

>100_bases
TCAAGAATTCGGCGGCCGACGACGACGAGGCGTCCGCAGGCCCCTCGGATGGTGAAAACGCGGAGCGGTAACGTCGGTTG
CCGACCAGCGAGAGGAACCT

Downstream 100 bases:

>100_bases
GCACCCGACCCGTACGGCCGGGTGATGATCTCGAGGTAGTGCCCGGCGGGGTCCTGGAAGTAGACGCCGCGGCCGCCGTC
GTGGTGGTTGATCTCTCCCG

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MDSEHDRQDDRPTTADGHPDPGVVKKAIAASAIGNATEWFDYGIYAYGVSYISAAIFPGEAGRATLLALMTFAVSFLVRP
LGGFVWGPLGDRLGRKRVLAITIVLMAGATFCVGLVPTYAAIGMWAPFLLVLLRMIQGFSTGGEYGGAATFMAEYAPNRR
RGLLGSFLEFGTLGGFAIGALLMLGCSLVLGDDQMHDWGWRLPFLLAAPLGLVGVYLRSRLEDTPVFRELEEKGEAEPQV
TAAFRDLLARYWRPILKLGGMVVALNVVNYTLLSYMPTYLETQIGLSADQSLLVPIIGMLAMMVFVPFVGLLSDRVGRKP
LWWFSLIGLFVAGVPMFLLMGTNLAGAVIGFAVLGLLYVPQLATISATFPAMFPTQVRYAGFAIAYNVSTSLFGGTAPAV
NEWLTNTTGDVLFPAYYMMAACVIGALALIKVPETARCPINGTVTPGTDEAPDPVPYEKV

Sequences:

>Translated_460_residues
MDSEHDRQDDRPTTADGHPDPGVVKKAIAASAIGNATEWFDYGIYAYGVSYISAAIFPGEAGRATLLALMTFAVSFLVRP
LGGFVWGPLGDRLGRKRVLAITIVLMAGATFCVGLVPTYAAIGMWAPFLLVLLRMIQGFSTGGEYGGAATFMAEYAPNRR
RGLLGSFLEFGTLGGFAIGALLMLGCSLVLGDDQMHDWGWRLPFLLAAPLGLVGVYLRSRLEDTPVFRELEEKGEAEPQV
TAAFRDLLARYWRPILKLGGMVVALNVVNYTLLSYMPTYLETQIGLSADQSLLVPIIGMLAMMVFVPFVGLLSDRVGRKP
LWWFSLIGLFVAGVPMFLLMGTNLAGAVIGFAVLGLLYVPQLATISATFPAMFPTQVRYAGFAIAYNVSTSLFGGTAPAV
NEWLTNTTGDVLFPAYYMMAACVIGALALIKVPETARCPINGTVTPGTDEAPDPVPYEKV
>Mature_460_residues
MDSEHDRQDDRPTTADGHPDPGVVKKAIAASAIGNATEWFDYGIYAYGVSYISAAIFPGEAGRATLLALMTFAVSFLVRP
LGGFVWGPLGDRLGRKRVLAITIVLMAGATFCVGLVPTYAAIGMWAPFLLVLLRMIQGFSTGGEYGGAATFMAEYAPNRR
RGLLGSFLEFGTLGGFAIGALLMLGCSLVLGDDQMHDWGWRLPFLLAAPLGLVGVYLRSRLEDTPVFRELEEKGEAEPQV
TAAFRDLLARYWRPILKLGGMVVALNVVNYTLLSYMPTYLETQIGLSADQSLLVPIIGMLAMMVFVPFVGLLSDRVGRKP
LWWFSLIGLFVAGVPMFLLMGTNLAGAVIGFAVLGLLYVPQLATISATFPAMFPTQVRYAGFAIAYNVSTSLFGGTAPAV
NEWLTNTTGDVLFPAYYMMAACVIGALALIKVPETARCPINGTVTPGTDEAPDPVPYEKV

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=440, Percent_Identity=42.5, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI1788942, Length=426, Percent_Identity=32.3943661971831, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1788292, Length=442, Percent_Identity=30.316742081448, Blast_Score=184, Evalue=9e-48,
Organism=Escherichia coli, GI1789941, Length=402, Percent_Identity=36.5671641791045, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI87082404, Length=384, Percent_Identity=23.6979166666667, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082231, Length=285, Percent_Identity=24.5614035087719, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49459; Mature: 49459

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]