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The map label for this gene is rph [H]

Identifier: 108800808

GI number: 108800808

Start: 4089896

End: 4090678

Strand: Reverse

Name: rph [H]

Synonym: Mmcs_3844

Alternate gene names: 108800808

Gene position: 4090678-4089896 (Counterclockwise)

Preceding gene: 108800809

Following gene: 108800807

Centisome position: 71.7

GC content: 69.73

Gene sequence:

>783_bases
GTGTCCAGACGAGAAGACGGTCGGCTTGACGACGAGTTGCGTCCGGTGCGCATCACCCGCGGTTTCACCTCGCATCCCGC
CGGTTCGGTGCTGGTCGAGTTCGGTGAGACCCGCGTGATGTGCACGGCCAGCGTCACCGAGGGAGTGCCGCGCTGGCGTA
AGGGAACGGGGCAGGGCTGGCTCACCGCCGAGTACGCGATGCTGCCCGCCGCCACGCACGATCGCTCCGACCGCGAATCG
GTCAAGGGCCGGGTCGGTGGCCGCACCCAGGAGATCAGCCGGTTGATCGGCCGGTCGCTGCGTGCGTGCATCGATCTGAA
TGCGTTGGGGGAGAACACAATCGCGATCGACTGCGATGTGCTGCAGGCCGACGGCGGCACCCGCACCGCGGCGATCACCG
GTGCGTACGTCGCGCTCGCCGATGCCGTGACCTACCTCGCCGCGGCCGAGAAGCTGTCCGATCCGCGGCCGCTGTCGTGT
GCGATCGCCGCGGTCAGCGTCGGCGTCGTCGACGGCCGGGTCCGCGTGGACCTGCCGTACTCGGAGGATTCGCGCGCCGA
GGTCGACATGAACGTCGTCGCCACCGACACCGGCACGCTGGTGGAGATCCAGGGCACCGGTGAGGGGGCGACGTTCCCGC
GCTCGACGCTGGACAAGCTGCTCGACCTCGCGCTCGCCTCCTGCGACCAGCTGTTCGTCGTGCAACGCGAAGCCCTCGAC
GCGCCGTATCCCGGTGCGCTCCCCGAGCCGACGTCGCCGCCGAAGAAGGCGTTCGGAAGCTGA

Upstream 100 bases:

>100_bases
GAGGCCAAGGCGGAGTTCGACGGTCCCGTGCACGCCGTGGTGTGCAACGAGTCGTTCGACGTCACGCATCGCTGACGGGG
CCACCGGTTAGGGTTGGCCC

Downstream 100 bases:

>100_bases
CGCAACTGTTGGTGGCCTCACGCAACCGCAAGAAGCTGGCCGAGCTTCGGCGGGTGCTCGACACCGCGGGGGTGTCCGGG
CTGACCCTGCTCTCGCTCGA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSRREDGRLDDELRPVRITRGFTSHPAGSVLVEFGETRVMCTASVTEGVPRWRKGTGQGWLTAEYAMLPAATHDRSDRES
VKGRVGGRTQEISRLIGRSLRACIDLNALGENTIAIDCDVLQADGGTRTAAITGAYVALADAVTYLAAAEKLSDPRPLSC
AIAAVSVGVVDGRVRVDLPYSEDSRAEVDMNVVATDTGTLVEIQGTGEGATFPRSTLDKLLDLALASCDQLFVVQREALD
APYPGALPEPTSPPKKAFGS

Sequences:

>Translated_260_residues
MSRREDGRLDDELRPVRITRGFTSHPAGSVLVEFGETRVMCTASVTEGVPRWRKGTGQGWLTAEYAMLPAATHDRSDRES
VKGRVGGRTQEISRLIGRSLRACIDLNALGENTIAIDCDVLQADGGTRTAAITGAYVALADAVTYLAAAEKLSDPRPLSC
AIAAVSVGVVDGRVRVDLPYSEDSRAEVDMNVVATDTGTLVEIQGTGEGATFPRSTLDKLLDLALASCDQLFVVQREALD
APYPGALPEPTSPPKKAFGS
>Mature_259_residues
SRREDGRLDDELRPVRITRGFTSHPAGSVLVEFGETRVMCTASVTEGVPRWRKGTGQGWLTAEYAMLPAATHDRSDRESV
KGRVGGRTQEISRLIGRSLRACIDLNALGENTIAIDCDVLQADGGTRTAAITGAYVALADAVTYLAAAEKLSDPRPLSCA
IAAVSVGVVDGRVRVDLPYSEDSRAEVDMNVVATDTGTLVEIQGTGEGATFPRSTLDKLLDLALASCDQLFVVQREALDA
PYPGALPEPTSPPKKAFGS

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=209, Percent_Identity=60.7655502392344, Blast_Score=257, Evalue=6e-70,
Organism=Caenorhabditis elegans, GI71981632, Length=252, Percent_Identity=25.7936507936508, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 27647; Mature: 27516

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRREDGRLDDELRPVRITRGFTSHPAGSVLVEFGETRVMCTASVTEGVPRWRKGTGQGW
CCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCEEEEEEEHHHHCCCHHHCCCCCCE
LTAEYAMLPAATHDRSDRESVKGRVGGRTQEISRLIGRSLRACIDLNALGENTIAIDCDV
EEEEEEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHEECCCCCCCEEEEEEEE
LQADGGTRTAAITGAYVALADAVTYLAAAEKLSDPRPLSCAIAAVSVGVVDGRVRVDLPY
EECCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCEEEEECCC
SEDSRAEVDMNVVATDTGTLVEIQGTGEGATFPRSTLDKLLDLALASCDQLFVVQREALD
CCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHEEEHHHHCC
APYPGALPEPTSPPKKAFGS
CCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
SRREDGRLDDELRPVRITRGFTSHPAGSVLVEFGETRVMCTASVTEGVPRWRKGTGQGW
CCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCEEEEEEEHHHHCCCHHHCCCCCCE
LTAEYAMLPAATHDRSDRESVKGRVGGRTQEISRLIGRSLRACIDLNALGENTIAIDCDV
EEEEEEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHEECCCCCCCEEEEEEEE
LQADGGTRTAAITGAYVALADAVTYLAAAEKLSDPRPLSCAIAAVSVGVVDGRVRVDLPY
EECCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCEEEEECCC
SEDSRAEVDMNVVATDTGTLVEIQGTGEGATFPRSTLDKLLDLALASCDQLFVVQREALD
CCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHEEEHHHHCC
APYPGALPEPTSPPKKAFGS
CCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA