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The map label for this gene is hsdS [H]

Identifier: 108800742

GI number: 108800742

Start: 4029860

End: 4031119

Strand: Reverse

Name: hsdS [H]

Synonym: Mmcs_3777

Alternate gene names: 108800742

Gene position: 4031119-4029860 (Counterclockwise)

Preceding gene: 108800743

Following gene: 108800741

Centisome position: 70.65

GC content: 56.83

Gene sequence:

>1260_bases
GTGAGTTGGGCTCAGGAGGTAACGCTCGCTGAACTCGCCGAGGGCGGGCTGTTCTCTGATGGAGACTGGGTTGAATCGAA
GGATCAAGATGCGAGCGGTGACGTACGGTTGACGCAGTTAGCCGACGTGGGCGTTGGCGAATTTCGTGATCGCTCTGACC
GCTGGATGCGGCGTGATCAAGCACACCGTCTTCGTTGCACGTTCCTGGAGGGTGACGACGTTCTCATTGCTCGGATGCCT
GATCCGATTGGACGTTCGTGCTTGGTGCCTTCGAGCGTCGGATCGGCCGTAACTGTTGTTGATGTCGCAATCCTCCGACT
TGCGCGGCGAGACGCAAACCCTAGGTACGTCATGTGGGCACTAAACTCCCCGAGGTTCCACTCCAAGGTTGTCGCCTTGC
AGTCGGGCACTACAAGGAAGCGAATATCCAGAAAAAATCTTGCGTCACTCACGATTCCATTACCAACCCTCGACGAACAA
AATCGCATTGTCGACCTTCTCGAAGACCACCTGTCGCGCCTCGATGCTGCTGAGTCCTCGCTCCGACTCGCGATGCAAAA
AGCTGATGCGATGACGACTGCATCTCTCGATCGGCAAACGACAGCGGGGTCCAGGGCTTGGCGCGATACAACCATCGGAG
CGATGGCGGAGTTGGTCGAGTATGGATCAAGTGCGAAATGTGCTGGACAAGCCGCTGACTCCGACGTCCCTGTGCTCCGC
ATGGGCAATATCCAGAATGGGAAGATCAATTGGACTGGATTGAAGTACTTGCCCGCTGGCCACGCGGAGTTCCCGAAGCT
GCTGCTGCAATCGGGTGATCTGGTTTTCAATCGTACGAACAGCGCTGAGCTAGTGGGTAAGTCAGCGGTCTTCGAAGACA
CTCGTGCCGCGTCATTCGCGTCGTATCTGATACGAGTGAGGTTCGGCCAGGAAGTGAATCCTGCGTGGGCGAACATGGTC
ATCAACAGCCCGGCAGGCCGACGATATGTGAAGTCGGTTGCATCGCAGCAAGTTGGTCAGGCGAACGTGAATGGGACGAA
ATTGAAAGCGTTTCCGTTACCGTTGCCACCCCTGGATGAGCAATGTCGTCGCGTCCGTGCTCATGATGAGGTCGTGGTGA
GTCGCGAACGACTTCACCATCAAATTGCAGATTTGGTGGTGAGGGCCGCTGGTCTCCGTCGCGCCCTCCTCGCCGCAGCA
TTCACCGGCCGCCTGACGAACTCTGCGGAAGGACTTCTCGAAGAACTCGAATCCGTATGA

Upstream 100 bases:

>100_bases
ATGCGCCGTTCATCGAACGTGGTGGGACCGACGGTGCGATCAGGGATTTGGGTGATCGGGCGGCGGAGTTGATTGATGAG
TTGAATGCGGAGCTGACGGC

Downstream 100 bases:

>100_bases
CCGGCAGGCAGATCAAGCTCTTCCTGGTCGACGGCAGCCCCGGCAGCCTCACCACCGCGGAGATCACCAACTGGACCGGC
CACGTCCTGTCGGCGCCACG

Product: restriction endonuclease S subunits-like protein

Products: NA

Alternate protein names: S.EcoKI; Type I restriction enzyme EcoKI specificity protein; S protein [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSWAQEVTLAELAEGGLFSDGDWVESKDQDASGDVRLTQLADVGVGEFRDRSDRWMRRDQAHRLRCTFLEGDDVLIARMP
DPIGRSCLVPSSVGSAVTVVDVAILRLARRDANPRYVMWALNSPRFHSKVVALQSGTTRKRISRKNLASLTIPLPTLDEQ
NRIVDLLEDHLSRLDAAESSLRLAMQKADAMTTASLDRQTTAGSRAWRDTTIGAMAELVEYGSSAKCAGQAADSDVPVLR
MGNIQNGKINWTGLKYLPAGHAEFPKLLLQSGDLVFNRTNSAELVGKSAVFEDTRAASFASYLIRVRFGQEVNPAWANMV
INSPAGRRYVKSVASQQVGQANVNGTKLKAFPLPLPPLDEQCRRVRAHDEVVVSRERLHHQIADLVVRAAGLRRALLAAA
FTGRLTNSAEGLLEELESV

Sequences:

>Translated_419_residues
MSWAQEVTLAELAEGGLFSDGDWVESKDQDASGDVRLTQLADVGVGEFRDRSDRWMRRDQAHRLRCTFLEGDDVLIARMP
DPIGRSCLVPSSVGSAVTVVDVAILRLARRDANPRYVMWALNSPRFHSKVVALQSGTTRKRISRKNLASLTIPLPTLDEQ
NRIVDLLEDHLSRLDAAESSLRLAMQKADAMTTASLDRQTTAGSRAWRDTTIGAMAELVEYGSSAKCAGQAADSDVPVLR
MGNIQNGKINWTGLKYLPAGHAEFPKLLLQSGDLVFNRTNSAELVGKSAVFEDTRAASFASYLIRVRFGQEVNPAWANMV
INSPAGRRYVKSVASQQVGQANVNGTKLKAFPLPLPPLDEQCRRVRAHDEVVVSRERLHHQIADLVVRAAGLRRALLAAA
FTGRLTNSAEGLLEELESV
>Mature_418_residues
SWAQEVTLAELAEGGLFSDGDWVESKDQDASGDVRLTQLADVGVGEFRDRSDRWMRRDQAHRLRCTFLEGDDVLIARMPD
PIGRSCLVPSSVGSAVTVVDVAILRLARRDANPRYVMWALNSPRFHSKVVALQSGTTRKRISRKNLASLTIPLPTLDEQN
RIVDLLEDHLSRLDAAESSLRLAMQKADAMTTASLDRQTTAGSRAWRDTTIGAMAELVEYGSSAKCAGQAADSDVPVLRM
GNIQNGKINWTGLKYLPAGHAEFPKLLLQSGDLVFNRTNSAELVGKSAVFEDTRAASFASYLIRVRFGQEVNPAWANMVI
NSPAGRRYVKSVASQQVGQANVNGTKLKAFPLPLPPLDEQCRRVRAHDEVVVSRERLHHQIADLVVRAAGLRRALLAAAF
TGRLTNSAEGLLEELESV

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=329, Percent_Identity=22.7963525835866, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 45966; Mature: 45835

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWAQEVTLAELAEGGLFSDGDWVESKDQDASGDVRLTQLADVGVGEFRDRSDRWMRRDQ
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHCCHHHHHHHHHH
AHRLRCTFLEGDDVLIARMPDPIGRSCLVPSSVGSAVTVVDVAILRLARRDANPRYVMWA
CCEEEEEEECCCCEEEEECCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
LNSPRFHSKVVALQSGTTRKRISRKNLASLTIPLPTLDEQNRIVDLLEDHLSRLDAAESS
ECCCCCHHEEEEECCCCHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
LRLAMQKADAMTTASLDRQTTAGSRAWRDTTIGAMAELVEYGSSAKCAGQAADSDVPVLR
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
MGNIQNGKINWTGLKYLPAGHAEFPKLLLQSGDLVFNRTNSAELVGKSAVFEDTRAASFA
ECCCCCCEEEEEEEEECCCCCHHHHHHHHHCCCEEEECCCCHHHHCCHHHHHHHHHHHHH
SYLIRVRFGQEVNPAWANMVINSPAGRRYVKSVASQQVGQANVNGTKLKAFPLPLPPLDE
HHHHHHCCCCCCCHHHHHEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHH
QCRRVRAHDEVVVSRERLHHQIADLVVRAAGLRRALLAAAFTGRLTNSAEGLLEELESV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SWAQEVTLAELAEGGLFSDGDWVESKDQDASGDVRLTQLADVGVGEFRDRSDRWMRRDQ
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHCCHHHHHHHHHH
AHRLRCTFLEGDDVLIARMPDPIGRSCLVPSSVGSAVTVVDVAILRLARRDANPRYVMWA
CCEEEEEEECCCCEEEEECCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
LNSPRFHSKVVALQSGTTRKRISRKNLASLTIPLPTLDEQNRIVDLLEDHLSRLDAAESS
ECCCCCHHEEEEECCCCHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
LRLAMQKADAMTTASLDRQTTAGSRAWRDTTIGAMAELVEYGSSAKCAGQAADSDVPVLR
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
MGNIQNGKINWTGLKYLPAGHAEFPKLLLQSGDLVFNRTNSAELVGKSAVFEDTRAASFA
ECCCCCCEEEEEEEEECCCCCHHHHHHHHHCCCEEEECCCCHHHHCCHHHHHHHHHHHHH
SYLIRVRFGQEVNPAWANMVINSPAGRRYVKSVASQQVGQANVNGTKLKAFPLPLPPLDE
HHHHHHCCCCCCCHHHHHEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHH
QCRRVRAHDEVVVSRERLHHQIADLVVRAAGLRRALLAAAFTGRLTNSAEGLLEELESV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6304321; 7610040; 9278503; 7739896 [H]