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The map label for this gene is hcaE [H]

Identifier: 108800682

GI number: 108800682

Start: 3969039

End: 3970316

Strand: Reverse

Name: hcaE [H]

Synonym: Mmcs_3717

Alternate gene names: 108800682

Gene position: 3970316-3969039 (Counterclockwise)

Preceding gene: 108800685

Following gene: 108800681

Centisome position: 69.59

GC content: 63.62

Gene sequence:

>1278_bases
ATGGCCCGGTTTCCCAAACCGCCAGAGGGCAGCTGGACGCAGCACTATCCGGAGCTCGGGACGGGGCCGGTGTCCTATGA
GGACTCGATCGATCCCGAGTTCTACGAGATCGAGCGTAAGGCCGTCTTCAAGCGCGCCTGGCTCAACGTGGGACGGGTCG
AACAGCTACCGCGCAAGGGCAGCTATTTCACCAAAGAACTGAAGGTCGCGAACACCTCGATCATCGTGGTGCGCACCGGC
AGTGGTGAGGTCAAGGCGTACCACAACATCTGCAGGCACCGCGGCAACAAGCTGGTGTGGAACGACATGCCGCTGGAAGA
GACGAGTGGAGTCTGCCGGCAGTTCACCTGCAAGTACCACGCCTGGCGCTACGACCTCGACGGCAACCTCACGTTCGTGC
AGCAGGAGGGCGAGTTCTTCGACCTCGACAAGAGCCGTTACGGGCTGGTCGGTGTCCACTGCGACGTGTGGGAGGGCTTC
ATCTTCGTCAACTTCGCCGAACAGCCCGAGCAGACACTGCGCGACTTCCTCGGACCGATGATCACGAATCTCGAAGGCTA
TCCGTTCGACCGGATGACCTCGCGGTTCCACTACCGCTCCGAGGTCAAGGCCAACTGGAAGCTCTACATGGACGCGTTCC
AGGAGTTCTACCACGCACCGGTGCTGCATGCGAACCAGTCACCGACCGCGTACTCGAAGGCCGCGGCCGAAGCCGGTTTC
GAAGCGCCGCACTACCGCATCGAAGGACCGCATCGGCTGGTCAGCACCTCGGGCGTGCGGGCCTGGGAGATGGCCGACGA
GATGCGCAAGCCGATCGAGGACATCTGCCAGAGCGGGCTGTTCGGCCCGTGGGACAAACCGGATCTGGGGGAGATGCCCG
CGGGGCTCAACCCGGCGAAATGCGATCCCTGGGGCCTGGATTCGTTCCAGCTGTTCCCCAACTTCGTCGTCCTGTTCTGG
GGGCAGGGCTGGTATCTCACGTACCACTACTGGCCGACGTCGCACAACACGCACATCTTCGAGGGCACGGTGTACTTCCC
GCAGCCCCGCACGGCGCGCGAACGCATCGCCCAGGAACTGGCCGCGGTGTCGTTCAAGGAGTACGGCCTGCAGGACGCCA
ACACCCTGGAGGCCACCCAGTCGATGATCGAGTCGCGCGTGCTCGACAACTTCGTGCTCTGCGATCAGGAGGTGCTGATC
CGCCACCTGCACAAGGAGACCGCGGCCTGGGTCGAGGAGTACCAGCGCAAGGCCCAGCACGTCGGGCAGGGGGTGTGA

Upstream 100 bases:

>100_bases
ATTGACCTGTGAAGCATATACCGTCGGCAGTGCACGCTCTGAGCGGAATGCCGAAAAGGCAGCTGTGCGTAGCTGTTTGC
CGCTAGACAGGAGACGACAC

Downstream 100 bases:

>100_bases
CCGTGTCCGACACCGCCACGACGACCGCGAAACTGCCGCCGGAATTCGCCGATCTCGAACAGTTCTCCGACTGGTGTCTG
GGCAGCGAGGCGGAACGCTA

Product: Rieske (2Fe-2S) domain-containing protein

Products: NA

Alternate protein names: Digoxigenin subunit alpha [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MARFPKPPEGSWTQHYPELGTGPVSYEDSIDPEFYEIERKAVFKRAWLNVGRVEQLPRKGSYFTKELKVANTSIIVVRTG
SGEVKAYHNICRHRGNKLVWNDMPLEETSGVCRQFTCKYHAWRYDLDGNLTFVQQEGEFFDLDKSRYGLVGVHCDVWEGF
IFVNFAEQPEQTLRDFLGPMITNLEGYPFDRMTSRFHYRSEVKANWKLYMDAFQEFYHAPVLHANQSPTAYSKAAAEAGF
EAPHYRIEGPHRLVSTSGVRAWEMADEMRKPIEDICQSGLFGPWDKPDLGEMPAGLNPAKCDPWGLDSFQLFPNFVVLFW
GQGWYLTYHYWPTSHNTHIFEGTVYFPQPRTARERIAQELAAVSFKEYGLQDANTLEATQSMIESRVLDNFVLCDQEVLI
RHLHKETAAWVEEYQRKAQHVGQGV

Sequences:

>Translated_425_residues
MARFPKPPEGSWTQHYPELGTGPVSYEDSIDPEFYEIERKAVFKRAWLNVGRVEQLPRKGSYFTKELKVANTSIIVVRTG
SGEVKAYHNICRHRGNKLVWNDMPLEETSGVCRQFTCKYHAWRYDLDGNLTFVQQEGEFFDLDKSRYGLVGVHCDVWEGF
IFVNFAEQPEQTLRDFLGPMITNLEGYPFDRMTSRFHYRSEVKANWKLYMDAFQEFYHAPVLHANQSPTAYSKAAAEAGF
EAPHYRIEGPHRLVSTSGVRAWEMADEMRKPIEDICQSGLFGPWDKPDLGEMPAGLNPAKCDPWGLDSFQLFPNFVVLFW
GQGWYLTYHYWPTSHNTHIFEGTVYFPQPRTARERIAQELAAVSFKEYGLQDANTLEATQSMIESRVLDNFVLCDQEVLI
RHLHKETAAWVEEYQRKAQHVGQGV
>Mature_424_residues
ARFPKPPEGSWTQHYPELGTGPVSYEDSIDPEFYEIERKAVFKRAWLNVGRVEQLPRKGSYFTKELKVANTSIIVVRTGS
GEVKAYHNICRHRGNKLVWNDMPLEETSGVCRQFTCKYHAWRYDLDGNLTFVQQEGEFFDLDKSRYGLVGVHCDVWEGFI
FVNFAEQPEQTLRDFLGPMITNLEGYPFDRMTSRFHYRSEVKANWKLYMDAFQEFYHAPVLHANQSPTAYSKAAAEAGFE
APHYRIEGPHRLVSTSGVRAWEMADEMRKPIEDICQSGLFGPWDKPDLGEMPAGLNPAKCDPWGLDSFQLFPNFVVLFWG
QGWYLTYHYWPTSHNTHIFEGTVYFPQPRTARERIAQELAAVSFKEYGLQDANTLEATQSMIESRVLDNFVLCDQEVLIR
HLHKETAAWVEEYQRKAQHVGQGV

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=214, Percent_Identity=32.2429906542056, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1788103, Length=196, Percent_Identity=29.5918367346939, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020875
- InterPro:   IPR017941
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.19 [H]

Molecular weight: Translated: 49181; Mature: 49050

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARFPKPPEGSWTQHYPELGTGPVSYEDSIDPEFYEIERKAVFKRAWLNVGRVEQLPRKG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCC
SYFTKELKVANTSIIVVRTGSGEVKAYHNICRHRGNKLVWNDMPLEETSGVCRQFTCKYH
CHHHHHEEEECEEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHEEE
AWRYDLDGNLTFVQQEGEFFDLDKSRYGLVGVHCDVWEGFIFVNFAEQPEQTLRDFLGPM
EEEEECCCCEEEEECCCCEEECCCCCCCEEEEEEECCCCEEEEEECCCHHHHHHHHHHHH
ITNLEGYPFDRMTSRFHYRSEVKANWKLYMDAFQEFYHAPVLHANQSPTAYSKAAAEAGF
HHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHCCC
EAPHYRIEGPHRLVSTSGVRAWEMADEMRKPIEDICQSGLFGPWDKPDLGEMPAGLNPAK
CCCCEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
CDPWGLDSFQLFPNFVVLFWGQGWYLTYHYWPTSHNTHIFEGTVYFPQPRTARERIAQEL
CCCCCCCHHHHCCCEEEEEECCCEEEEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHH
AAVSFKEYGLQDANTLEATQSMIESRVLDNFVLCDQEVLIRHLHKETAAWVEEYQRKAQH
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
VGQGV
HCCCC
>Mature Secondary Structure 
ARFPKPPEGSWTQHYPELGTGPVSYEDSIDPEFYEIERKAVFKRAWLNVGRVEQLPRKG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCC
SYFTKELKVANTSIIVVRTGSGEVKAYHNICRHRGNKLVWNDMPLEETSGVCRQFTCKYH
CHHHHHEEEECEEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHEEE
AWRYDLDGNLTFVQQEGEFFDLDKSRYGLVGVHCDVWEGFIFVNFAEQPEQTLRDFLGPM
EEEEECCCCEEEEECCCCEEECCCCCCCEEEEEEECCCCEEEEEECCCHHHHHHHHHHHH
ITNLEGYPFDRMTSRFHYRSEVKANWKLYMDAFQEFYHAPVLHANQSPTAYSKAAAEAGF
HHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHCCC
EAPHYRIEGPHRLVSTSGVRAWEMADEMRKPIEDICQSGLFGPWDKPDLGEMPAGLNPAK
CCCCEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
CDPWGLDSFQLFPNFVVLFWGQGWYLTYHYWPTSHNTHIFEGTVYFPQPRTARERIAQEL
CCCCCCCHHHHCCCEEEEEECCCEEEEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHH
AAVSFKEYGLQDANTLEATQSMIESRVLDNFVLCDQEVLIRHLHKETAAWVEEYQRKAQH
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
VGQGV
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]