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The map label for this gene is citE [H]

Identifier: 108800593

GI number: 108800593

Start: 3872195

End: 3873013

Strand: Reverse

Name: citE [H]

Synonym: Mmcs_3627

Alternate gene names: 108800593

Gene position: 3873013-3872195 (Counterclockwise)

Preceding gene: 108800594

Following gene: 108800592

Centisome position: 67.88

GC content: 70.33

Gene sequence:

>819_bases
GTGACGCTCGGCAACAACGGGCCCGGGTGGCTGTTCTGCCCGGCCGACCGGCCGGAGCGCTTCGAGAAGGCTGCCGCGGC
AGCCGATGTGGTGATCCTCGACCTCGAGGACGGGGTGGCTGCCAGGGACCGGGAGGCGGCGCGCGCGGCGCTCGTCGACA
CCCCGCTCGACCCGTCGCGCACCGTCGTCCGGGTGAACCCGACCACCACGCCCGACCATGCCCTCGACCTGCAGGCGCTG
GCACGGACCGACTACACGACCGTCATGCTCGCCAAGACCGAGAGCGCAGAACAGGTGAGCGCACTGGCGCCGCTGGAGGT
GGTGGCGCTGATCGAAACCCCGCTGGGGGCGCTCACCGTCGTCGAATCGAGTCGCGTGGACAACACCGTCGCGGTGATGT
GGGGCGCCGAGGATCTGCTGGCGGTCACCGGCGGCACGTCGAGTCGGTGGCCCGACGGCTCCTACCGGGACTTCGCCGTG
CACGTCCGCTCCCGGTCGCTGCTGGCCGCCAAGGCCTACGGCCGGATGGCACTGGACTCGGTCTACCTCGACATCAAGGA
TCTCGACGGGCTGCGGGCCGAAACCGACGATGCGGTCGCGGTCGGATTCGACGCCAAGGTCGCCATCCACCCCACCCAGG
TCGCGGTGATCCGCGACGGCTACCGGCCCACCGACGAACAGGTCGACTGGGCCCGTCGCGTCCTGGACACGGCGCACGAT
GAACGCGGCGTCTTCCAGTTCGAGGGCATAATGGTGGATGCCCCAGTGCTGAGGCGAGCAGAGCGCATCGTCGCGTTGGC
GCCACACCCAGGCAGCTGA

Upstream 100 bases:

>100_bases
TCGTGACCTTCTCCCACGTCGGGCGCAATCAGCACGGCGACGTCGTCGCGACCGCGTCACGAAAGACCATGGTGCGCAAG
CGACCTGAAGGAGACCCGCA

Downstream 100 bases:

>100_bases
CCGGCAGCCCTACACCAGGGTTCCTGCTCTGACCGCCACACGCTGAGCACCTGAAGTCGTCGGACGGCGAAACATGCCGT
CGATGCGACCTGAAGGAGGC

Product: HpcH/HpaI aldolase

Products: Acetate; Oxaloacetate [C]

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTLGNNGPGWLFCPADRPERFEKAAAAADVVILDLEDGVAARDREAARAALVDTPLDPSRTVVRVNPTTTPDHALDLQAL
ARTDYTTVMLAKTESAEQVSALAPLEVVALIETPLGALTVVESSRVDNTVAVMWGAEDLLAVTGGTSSRWPDGSYRDFAV
HVRSRSLLAAKAYGRMALDSVYLDIKDLDGLRAETDDAVAVGFDAKVAIHPTQVAVIRDGYRPTDEQVDWARRVLDTAHD
ERGVFQFEGIMVDAPVLRRAERIVALAPHPGS

Sequences:

>Translated_272_residues
MTLGNNGPGWLFCPADRPERFEKAAAAADVVILDLEDGVAARDREAARAALVDTPLDPSRTVVRVNPTTTPDHALDLQAL
ARTDYTTVMLAKTESAEQVSALAPLEVVALIETPLGALTVVESSRVDNTVAVMWGAEDLLAVTGGTSSRWPDGSYRDFAV
HVRSRSLLAAKAYGRMALDSVYLDIKDLDGLRAETDDAVAVGFDAKVAIHPTQVAVIRDGYRPTDEQVDWARRVLDTAHD
ERGVFQFEGIMVDAPVLRRAERIVALAPHPGS
>Mature_271_residues
TLGNNGPGWLFCPADRPERFEKAAAAADVVILDLEDGVAARDREAARAALVDTPLDPSRTVVRVNPTTTPDHALDLQALA
RTDYTTVMLAKTESAEQVSALAPLEVVALIETPLGALTVVESSRVDNTVAVMWGAEDLLAVTGGTSSRWPDGSYRDFAVH
VRSRSLLAAKAYGRMALDSVYLDIKDLDGLRAETDDAVAVGFDAKVAIHPTQVAVIRDGYRPTDEQVDWARRVLDTAHDE
RGVFQFEGIMVDAPVLRRAERIVALAPHPGS

Specific function: May play a role in fatty acid biosynthesis (Potential) [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit-like subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=291, Percent_Identity=31.2714776632302, Blast_Score=139, Evalue=3e-33,
Organism=Escherichia coli, GI87081764, Length=268, Percent_Identity=32.4626865671642, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17557458, Length=140, Percent_Identity=37.1428571428571, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6 [C]

Molecular weight: Translated: 29338; Mature: 29207

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLGNNGPGWLFCPADRPERFEKAAAAADVVILDLEDGVAARDREAARAALVDTPLDPSR
CCCCCCCCEEEEECCCCHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHEEECCCCCCCC
TVVRVNPTTTPDHALDLQALARTDYTTVMLAKTESAEQVSALAPLEVVALIETPLGALTV
EEEEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCEEEEEHHCCCCEEEE
VESSRVDNTVAVMWGAEDLLAVTGGTSSRWPDGSYRDFAVHVRSRSLLAAKAYGRMALDS
EECCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCEEEEEEECCCEEEHHHHCCCEEEE
VYLDIKDLDGLRAETDDAVAVGFDAKVAIHPTQVAVIRDGYRPTDEQVDWARRVLDTAHD
EEEEEECCCCCCCCCCCEEEEECCCEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHCCC
ERGVFQFEGIMVDAPVLRRAERIVALAPHPGS
CCCEEEECCEEECCHHHHCCCEEEEECCCCCC
>Mature Secondary Structure 
TLGNNGPGWLFCPADRPERFEKAAAAADVVILDLEDGVAARDREAARAALVDTPLDPSR
CCCCCCCEEEEECCCCHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHEEECCCCCCCC
TVVRVNPTTTPDHALDLQALARTDYTTVMLAKTESAEQVSALAPLEVVALIETPLGALTV
EEEEECCCCCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCEEEEEHHCCCCEEEE
VESSRVDNTVAVMWGAEDLLAVTGGTSSRWPDGSYRDFAVHVRSRSLLAAKAYGRMALDS
EECCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCEEEEEEECCCEEEHHHHCCCEEEE
VYLDIKDLDGLRAETDDAVAVGFDAKVAIHPTQVAVIRDGYRPTDEQVDWARRVLDTAHD
EEEEEECCCCCCCCCCCEEEEECCCEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHCCC
ERGVFQFEGIMVDAPVLRRAERIVALAPHPGS
CCCEEEECCEEECCHHHHCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: Citrate [C]

Specific reaction: Citrate --> Acetate + Oxaloacetate [C]

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]