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The map label for this gene is nei [C]

Identifier: 108800564

GI number: 108800564

Start: 3837618

End: 3838424

Strand: Reverse

Name: nei [C]

Synonym: Mmcs_3598

Alternate gene names: 108800564

Gene position: 3838424-3837618 (Counterclockwise)

Preceding gene: 108800565

Following gene: 108800563

Centisome position: 67.28

GC content: 71.25

Gene sequence:

>807_bases
ATGCCCGAGGGCCATACGCTGCACCGGCTCGCCCGGCTGCACCAGAAGCGGTACAAGGGCCAGCGGGTGCGGGTGAGCAG
TCCGCAGGGCCGGTTCGCCGAGGGCGCGGCGATGGTGGACGGTCGCACCTTCACCGGTGCCAGCGCCTGGGGTAAGCACC
TGTTCCACCACTACCGCGGCGGCCGCATCATCCACGTGCACCTGGGCCTGTACGGACGGTTCGACGAGTTCGCGGTGTCG
GCCGACGATCCGCCACCCGATCCCGTCGGACAGGTGCGCATGCGCATGGTCGGTGCCGGCCACGGCACCGACCTGCGCGG
CCCCACCGCCTGCGAGATCGTCGACGAGGCGCAGGTGTCCGACATCCTGGCCCGGCTCGGACCCGACCCGCTGCGCCGCG
ACGCCGATCCGGCGCCCGCGTGGACGCGGCTCAACCGGTCGCGCAGGCCCGTGGGCGCGCTGCTGATGGACCAAACGGTG
ATCGCCGGCGTCGGCAACGTCTACCGCAGCGAACTGCTGTACCGCCACCGCATCGACCCCTACCGGTTGGGCACCAACCT
CGAGCCGGGGGAGTTCGACGCGATGTGGACCGACCTCGTCGAGTTGATGAAAGTCGGTGTGCGGCGCGGCAAGATCGTCA
CCGTCCGGCCCGAGGACGATCACGGGGCACCGTCGTACCGCACCGGCCGGCCGCGCACGTACGTGTATCGCCGGGCGCTC
GAACCGTGCCGGATCTGCGGGACGCCCGTGAACACCGCCGTCCTGGAGGGCCGCAACCTGTTCTGGTGCCCGACCTGCCA
GAGGTGA

Upstream 100 bases:

>100_bases
GTGGTCGAAAGCCGAACGCCACCAACGGCGTATCGACATCCTCTCCGAATACGAGCGGACCCACGAAGCGCCGCCGGTGC
CCGGCGCCCCTGCCTGACGG

Downstream 100 bases:

>100_bases
GGCGAAGCCCGCATACGCGCGGGTTTGCGGCGACAATCGCCCGGTGGAACTCATCCTCGTCGTCGTCGGCGCCATCGTGG
TCACCGCCATCGCCCACCGC

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Putative DNA-(apurinic or apyrimidinic site) lyase Rv2464c/MT2539; Putative AP lyase Rv2464c/MT2539 [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MPEGHTLHRLARLHQKRYKGQRVRVSSPQGRFAEGAAMVDGRTFTGASAWGKHLFHHYRGGRIIHVHLGLYGRFDEFAVS
ADDPPPDPVGQVRMRMVGAGHGTDLRGPTACEIVDEAQVSDILARLGPDPLRRDADPAPAWTRLNRSRRPVGALLMDQTV
IAGVGNVYRSELLYRHRIDPYRLGTNLEPGEFDAMWTDLVELMKVGVRRGKIVTVRPEDDHGAPSYRTGRPRTYVYRRAL
EPCRICGTPVNTAVLEGRNLFWCPTCQR

Sequences:

>Translated_268_residues
MPEGHTLHRLARLHQKRYKGQRVRVSSPQGRFAEGAAMVDGRTFTGASAWGKHLFHHYRGGRIIHVHLGLYGRFDEFAVS
ADDPPPDPVGQVRMRMVGAGHGTDLRGPTACEIVDEAQVSDILARLGPDPLRRDADPAPAWTRLNRSRRPVGALLMDQTV
IAGVGNVYRSELLYRHRIDPYRLGTNLEPGEFDAMWTDLVELMKVGVRRGKIVTVRPEDDHGAPSYRTGRPRTYVYRRAL
EPCRICGTPVNTAVLEGRNLFWCPTCQR
>Mature_267_residues
PEGHTLHRLARLHQKRYKGQRVRVSSPQGRFAEGAAMVDGRTFTGASAWGKHLFHHYRGGRIIHVHLGLYGRFDEFAVSA
DDPPPDPVGQVRMRMVGAGHGTDLRGPTACEIVDEAQVSDILARLGPDPLRRDADPAPAWTRLNRSRRPVGALLMDQTVI
AGVGNVYRSELLYRHRIDPYRLGTNLEPGEFDAMWTDLVELMKVGVRRGKIVTVRPEDDHGAPSYRTGRPRTYVYRRALE
PCRICGTPVNTAVLEGRNLFWCPTCQR

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA b

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1786932, Length=276, Percent_Identity=26.8115942028986, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790066, Length=220, Percent_Identity=28.1818181818182, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR012319
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663 [H]

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 30107; Mature: 29976

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS01242 ZF_FPG_1 ; PS51066 ZF_FPG_2 ; PS00485 A_DEAMINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEGHTLHRLARLHQKRYKGQRVRVSSPQGRFAEGAAMVDGRTFTGASAWGKHLFHHYRG
CCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEECCCEECCHHHHHHHHHHHHCC
GRIIHVHLGLYGRFDEFAVSADDPPPDPVGQVRMRMVGAGHGTDLRGPTACEIVDEAQVS
CEEEEEEECCCCCCHHEEECCCCCCCCCHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHH
DILARLGPDPLRRDADPAPAWTRLNRSRRPVGALLMDQTVIAGVGNVYRSELLYRHRIDP
HHHHHCCCCHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YRLGTNLEPGEFDAMWTDLVELMKVGVRRGKIVTVRPEDDHGAPSYRTGRPRTYVYRRAL
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHC
EPCRICGTPVNTAVLEGRNLFWCPTCQR
CHHHHCCCCCCCEEECCCCEEECCCCCC
>Mature Secondary Structure 
PEGHTLHRLARLHQKRYKGQRVRVSSPQGRFAEGAAMVDGRTFTGASAWGKHLFHHYRG
CCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEECCCEECCHHHHHHHHHHHHCC
GRIIHVHLGLYGRFDEFAVSADDPPPDPVGQVRMRMVGAGHGTDLRGPTACEIVDEAQVS
CEEEEEEECCCCCCHHEEECCCCCCCCCHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHH
DILARLGPDPLRRDADPAPAWTRLNRSRRPVGALLMDQTVIAGVGNVYRSELLYRHRIDP
HHHHHCCCCHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YRLGTNLEPGEFDAMWTDLVELMKVGVRRGKIVTVRPEDDHGAPSYRTGRPRTYVYRRAL
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHC
EPCRICGTPVNTAVLEGRNLFWCPTCQR
CHHHHCCCCCCCEEECCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]