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The map label for this gene is yoaE [H]

Identifier: 108800560

GI number: 108800560

Start: 3831535

End: 3833691

Strand: Reverse

Name: yoaE [H]

Synonym: Mmcs_3594

Alternate gene names: 108800560

Gene position: 3833691-3831535 (Counterclockwise)

Preceding gene: 108800563

Following gene: 108800559

Centisome position: 67.19

GC content: 66.25

Gene sequence:

>2157_bases
GTGAAGGAACTCACCATCGTCCGAGGCGCCTGCCCGCACGACTGCCCCGACACGTGCGCGATGCTCTACCACGTCGAGGA
CGGGAAACTCGTTGACGTGACGGGAGATCCGGACCACCCGATGACCAGGGGCGGTCTGTGCGTCAAGGTCAAGAACTTCC
ACGAGCACCACTACCAGCCCGACCGTCTGCTGTACCCGATGCGCCGGGTGGGGCCCAAGGGTTCCGGTGCGTTCGAGCGG
ATCTCGTGGGACGAGGCGCTCGCGGAGATCAAACAGAGGTGGACCGCGATCATCGACGAGTACGGCAGCCAGGCGATCAT
GCCGCACGCCTACCTCGGCCACCAGGGTGTGCTCAATGGTCTGACCTCGGGTGACGCGTTCTTCAACCGGCTCGGATCGA
CGGTGGCGGAAAAGACGTACTGCGAGTCCGGATCGTCGACGGCGTGGCATATGACCGTCGGCGGGTCGGGTGGTCTCGAC
GTCGAGTCGATGGCGCATTCGAAGTACATCATCGTGTGGGGCATGAACATGACCAGCACGAACCTGCACGGCTGGCCGTT
CCTGCTGGAGGCGCGCAAGAACAACGGCGCGAAGATCGTCGTCATCGATCCGGTGCGCAACCGGACTGCGCGACAGGCTG
ATTGGCACATCCGCATCCGGCCCGGCACCGACGGCGCCCTCGCCATGGGCATGATCCACGAGATCATCGCGCAGGGGCTG
ACCGACACCGACTACATCGAGCGCTACACCGTCGGATTCGACGAACTCGCCGCGCGTGCGGCGAACTATCCGCCGGAGCG
GGTCGAGGAGATCACCGGTGTGCCCGCCGACGACGTCCGCACGCTGGCCCGCGAGTACGCCACGAGCCAGCCCGCGGCGA
TCCGGCAGGGGGTGGCGTTGGAACGCAGCCGCGGCGGGGGACAGGCGATCCGCGCGATCACCAGCCTGCCGGCACTGGTG
GGCGCCTGGCGCCACGTCGGTGGCGGCACGATGGAGATGCCGATCTGGGAGTTCCCCACCCGGTTCGACAAGATCTGCAT
GCCGGAGTGGATTCCCGAGGGCACCCGCGTGGTCAACGAACTCGACCTCGGGATGGCGCTGACCGGTGAACTCGAACTCG
ACCCGCCCATCAGATCGCTGTTCGTCTACAACTCCAACCCGGTGTCACAGGGTCCCGCGCAGGAGAAGACGATGCGCGGG
CTGATGCGCGACGACCTGTTCACCGTCGTCAGCGAGCACTTCATCACCGACACCGCGAAATACGCCGATCTGGTGCTGCC
TGCCACCATGCAGGCCGAGCAGCTCGACATCATGGTGACCTGGGGCCACCTCTACATCTCGCTCAACCAGCCCGCGATCG
CTCCGCCGGGTGACGCGATCCCCAACGTCGAACTGTTCCGCCGGCTGGCGCGGACCATGGAGTTCGACGAGGAGTCGATG
GCCTACTGGAACCGCACCGACCGCGAGATGCTCATCGACTTCCACGATTGGGAAGCGCCTGCGCTGCAGGGCATCACGTA
CGAGAAGCTCGAAGAGGTCGGGTGGATGCGGCTCAACGTCGGCAGCCCGGACACCAGGGCCCCGCACGCCGAGGGCAACT
TCCCCACCCCGTCGGGCAAGTGCGAGTTCAAGTCCAGCCTCGCCGAGGGTGGCAACTTCGTGGTCCCGGTTTGGCGGTCG
ATGTACGAGGCGATGCAGCCGGGCGGATACGTGGACCCGGTACCCGACTACGTTCCGCCCTTCGAATCCCCGCAGTCGAA
TCCGGAACTGGCGCAACGGTTCCCGTTGAGCATCATCTCGCCGAAACCGCACGCCTTCCTCAACAGTCAGTACGGCAACG
CGCCGGACAAGCAGCGGGTCCAGGGCGGCCAGCGGGTGTTCATCCATCCCGACGACGCCGCCGCACGCGGTATCGCCGAA
GGGGAGATGGTGCGGGTGTTCAACGACCGCGGATCCTTCGTGGGACCGGCCACCTACGAGCCGGACCTGATGCCGGGTCT
GGTGATGGCCAATGTCGGACACTGGCAGAGCGGGGGTTCGGGCACGACCGTGAACGCGATCACCGCCGACCGGCACTGCG
GTCTGGGCAACGCGGGCGTGTACGGCGACAACCTGGTGGAGGTCCAGAAGGTCGCGGACGAGGCCGTCGCGAGCTGA

Upstream 100 bases:

>100_bases
CCGGGGGAGGTTCGGTCACAGCACCTTAATGTCCGTGCAATTGGCGGGCCACAAACCTCGGCATAACTGGTAGCACCCAC
TGCAGCCCAGGAGGACCCCC

Downstream 100 bases:

>100_bases
CGCCGATTGTCTTACCGATCCGCGGAATCCGGAGCGCAGACCGGGGAGCGGCGTAGTGTGCGGCTAGCGGGTTCGCCGGT
CTGTGAGGAGGCGGAACATG

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MKELTIVRGACPHDCPDTCAMLYHVEDGKLVDVTGDPDHPMTRGGLCVKVKNFHEHHYQPDRLLYPMRRVGPKGSGAFER
ISWDEALAEIKQRWTAIIDEYGSQAIMPHAYLGHQGVLNGLTSGDAFFNRLGSTVAEKTYCESGSSTAWHMTVGGSGGLD
VESMAHSKYIIVWGMNMTSTNLHGWPFLLEARKNNGAKIVVIDPVRNRTARQADWHIRIRPGTDGALAMGMIHEIIAQGL
TDTDYIERYTVGFDELAARAANYPPERVEEITGVPADDVRTLAREYATSQPAAIRQGVALERSRGGGQAIRAITSLPALV
GAWRHVGGGTMEMPIWEFPTRFDKICMPEWIPEGTRVVNELDLGMALTGELELDPPIRSLFVYNSNPVSQGPAQEKTMRG
LMRDDLFTVVSEHFITDTAKYADLVLPATMQAEQLDIMVTWGHLYISLNQPAIAPPGDAIPNVELFRRLARTMEFDEESM
AYWNRTDREMLIDFHDWEAPALQGITYEKLEEVGWMRLNVGSPDTRAPHAEGNFPTPSGKCEFKSSLAEGGNFVVPVWRS
MYEAMQPGGYVDPVPDYVPPFESPQSNPELAQRFPLSIISPKPHAFLNSQYGNAPDKQRVQGGQRVFIHPDDAAARGIAE
GEMVRVFNDRGSFVGPATYEPDLMPGLVMANVGHWQSGGSGTTVNAITADRHCGLGNAGVYGDNLVEVQKVADEAVAS

Sequences:

>Translated_718_residues
MKELTIVRGACPHDCPDTCAMLYHVEDGKLVDVTGDPDHPMTRGGLCVKVKNFHEHHYQPDRLLYPMRRVGPKGSGAFER
ISWDEALAEIKQRWTAIIDEYGSQAIMPHAYLGHQGVLNGLTSGDAFFNRLGSTVAEKTYCESGSSTAWHMTVGGSGGLD
VESMAHSKYIIVWGMNMTSTNLHGWPFLLEARKNNGAKIVVIDPVRNRTARQADWHIRIRPGTDGALAMGMIHEIIAQGL
TDTDYIERYTVGFDELAARAANYPPERVEEITGVPADDVRTLAREYATSQPAAIRQGVALERSRGGGQAIRAITSLPALV
GAWRHVGGGTMEMPIWEFPTRFDKICMPEWIPEGTRVVNELDLGMALTGELELDPPIRSLFVYNSNPVSQGPAQEKTMRG
LMRDDLFTVVSEHFITDTAKYADLVLPATMQAEQLDIMVTWGHLYISLNQPAIAPPGDAIPNVELFRRLARTMEFDEESM
AYWNRTDREMLIDFHDWEAPALQGITYEKLEEVGWMRLNVGSPDTRAPHAEGNFPTPSGKCEFKSSLAEGGNFVVPVWRS
MYEAMQPGGYVDPVPDYVPPFESPQSNPELAQRFPLSIISPKPHAFLNSQYGNAPDKQRVQGGQRVFIHPDDAAARGIAE
GEMVRVFNDRGSFVGPATYEPDLMPGLVMANVGHWQSGGSGTTVNAITADRHCGLGNAGVYGDNLVEVQKVADEAVAS
>Mature_718_residues
MKELTIVRGACPHDCPDTCAMLYHVEDGKLVDVTGDPDHPMTRGGLCVKVKNFHEHHYQPDRLLYPMRRVGPKGSGAFER
ISWDEALAEIKQRWTAIIDEYGSQAIMPHAYLGHQGVLNGLTSGDAFFNRLGSTVAEKTYCESGSSTAWHMTVGGSGGLD
VESMAHSKYIIVWGMNMTSTNLHGWPFLLEARKNNGAKIVVIDPVRNRTARQADWHIRIRPGTDGALAMGMIHEIIAQGL
TDTDYIERYTVGFDELAARAANYPPERVEEITGVPADDVRTLAREYATSQPAAIRQGVALERSRGGGQAIRAITSLPALV
GAWRHVGGGTMEMPIWEFPTRFDKICMPEWIPEGTRVVNELDLGMALTGELELDPPIRSLFVYNSNPVSQGPAQEKTMRG
LMRDDLFTVVSEHFITDTAKYADLVLPATMQAEQLDIMVTWGHLYISLNQPAIAPPGDAIPNVELFRRLARTMEFDEESM
AYWNRTDREMLIDFHDWEAPALQGITYEKLEEVGWMRLNVGSPDTRAPHAEGNFPTPSGKCEFKSSLAEGGNFVVPVWRS
MYEAMQPGGYVDPVPDYVPPFESPQSNPELAQRFPLSIISPKPHAFLNSQYGNAPDKQRVQGGQRVFIHPDDAAARGIAE
GEMVRVFNDRGSFVGPATYEPDLMPGLVMANVGHWQSGGSGTTVNAITADRHCGLGNAGVYGDNLVEVQKVADEAVAS

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=781, Percent_Identity=29.5774647887324, Blast_Score=274, Evalue=2e-74,
Organism=Escherichia coli, GI1787870, Length=776, Percent_Identity=30.2835051546392, Blast_Score=273, Evalue=3e-74,
Organism=Escherichia coli, GI171474008, Length=787, Percent_Identity=29.4790343074968, Blast_Score=272, Evalue=4e-74,
Organism=Escherichia coli, GI3868721, Length=589, Percent_Identity=27.6740237691002, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI87081994, Length=776, Percent_Identity=24.8711340206186, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1787231, Length=725, Percent_Identity=24.1379310344828, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI145693196, Length=745, Percent_Identity=24.5637583892617, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1788534, Length=734, Percent_Identity=22.0708446866485, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI3868719, Length=399, Percent_Identity=24.3107769423559, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI3868720, Length=399, Percent_Identity=24.0601503759398, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 79148; Mature: 79148

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELTIVRGACPHDCPDTCAMLYHVEDGKLVDVTGDPDHPMTRGGLCVKVKNFHEHHYQP
CCCEEEEECCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCEEEEEECHHHHCCCC
DRLLYPMRRVGPKGSGAFERISWDEALAEIKQRWTAIIDEYGSQAIMPHAYLGHQGVLNG
HHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHC
LTSGDAFFNRLGSTVAEKTYCESGSSTAWHMTVGGSGGLDVESMAHSKYIIVWGMNMTST
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHCCCCEEEEEECCCCCC
NLHGWPFLLEARKNNGAKIVVIDPVRNRTARQADWHIRIRPGTDGALAMGMIHEIIAQGL
CCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCC
TDTDYIERYTVGFDELAARAANYPPERVEEITGVPADDVRTLAREYATSQPAAIRQGVAL
CCHHHHHHHHCCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHCCCE
ERSRGGGQAIRAITSLPALVGAWRHVGGGTMEMPIWEFPTRFDKICMPEWIPEGTRVVNE
ECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHCCCHHHHHCCCCCCCCHHHHHHH
LDLGMALTGELELDPPIRSLFVYNSNPVSQGPAQEKTMRGLMRDDLFTVVSEHFITDTAK
HCCCEEEECCEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YADLVLPATMQAEQLDIMVTWGHLYISLNQPAIAPPGDAIPNVELFRRLARTMEFDEESM
HHHEEECCCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHH
AYWNRTDREMLIDFHDWEAPALQGITYEKLEEVGWMRLNVGSPDTRAPHAEGNFPTPSGK
HHHCCCCCEEEEEECCCCCHHHCCCCHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCC
CEFKSSLAEGGNFVVPVWRSMYEAMQPGGYVDPVPDYVPPFESPQSNPELAQRFPLSIIS
CHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEC
PKPHAFLNSQYGNAPDKQRVQGGQRVFIHPDDAAARGIAEGEMVRVFNDRGSFVGPATYE
CCCCCEECCCCCCCCCHHHHCCCCEEEEECCHHHHCCCCCCCEEEEECCCCCCCCCCCCC
PDLMPGLVMANVGHWQSGGSGTTVNAITADRHCGLGNAGVYGDNLVEVQKVADEAVAS
CCCCCCHHEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKELTIVRGACPHDCPDTCAMLYHVEDGKLVDVTGDPDHPMTRGGLCVKVKNFHEHHYQP
CCCEEEEECCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCEEEEEECHHHHCCCC
DRLLYPMRRVGPKGSGAFERISWDEALAEIKQRWTAIIDEYGSQAIMPHAYLGHQGVLNG
HHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHC
LTSGDAFFNRLGSTVAEKTYCESGSSTAWHMTVGGSGGLDVESMAHSKYIIVWGMNMTST
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHCCCCEEEEEECCCCCC
NLHGWPFLLEARKNNGAKIVVIDPVRNRTARQADWHIRIRPGTDGALAMGMIHEIIAQGL
CCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCC
TDTDYIERYTVGFDELAARAANYPPERVEEITGVPADDVRTLAREYATSQPAAIRQGVAL
CCHHHHHHHHCCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHCCCE
ERSRGGGQAIRAITSLPALVGAWRHVGGGTMEMPIWEFPTRFDKICMPEWIPEGTRVVNE
ECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHCCCHHHHHCCCCCCCCHHHHHHH
LDLGMALTGELELDPPIRSLFVYNSNPVSQGPAQEKTMRGLMRDDLFTVVSEHFITDTAK
HCCCEEEECCEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YADLVLPATMQAEQLDIMVTWGHLYISLNQPAIAPPGDAIPNVELFRRLARTMEFDEESM
HHHEEECCCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHH
AYWNRTDREMLIDFHDWEAPALQGITYEKLEEVGWMRLNVGSPDTRAPHAEGNFPTPSGK
HHHCCCCCEEEEEECCCCCHHHCCCCHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCC
CEFKSSLAEGGNFVVPVWRSMYEAMQPGGYVDPVPDYVPPFESPQSNPELAQRFPLSIIS
CHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEC
PKPHAFLNSQYGNAPDKQRVQGGQRVFIHPDDAAARGIAEGEMVRVFNDRGSFVGPATYE
CCCCCEECCCCCCCCCHHHHCCCCEEEEECCHHHHCCCCCCCEEEEECCCCCCCCCCCCC
PDLMPGLVMANVGHWQSGGSGTTVNAITADRHCGLGNAGVYGDNLVEVQKVADEAVAS
CCCCCCHHEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]