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The map label for this gene is 108800297

Identifier: 108800297

GI number: 108800297

Start: 3532087

End: 3533529

Strand: Reverse

Name: 108800297

Synonym: Mmcs_3331

Alternate gene names: NA

Gene position: 3533529-3532087 (Counterclockwise)

Preceding gene: 108800299

Following gene: 108800296

Centisome position: 61.93

GC content: 67.22

Gene sequence:

>1443_bases
GTGTTAAGTGGATTTGCTCACACCGTCGTCCCGGACCCCTACGCTGGGCGTATGCAACGGCTCAGCGGTCTCGACGCCAG
TTTCCTGTATCTCGAAACCGCACAGCAGCTCCTGCACGTGTGCTCGATCCTCGAGCTGGACACCACGACGATGCCCGGCG
GCTACACCTTCGCCAAACTGCGCGACGCCCTGTCGCGGCGCATCGAGTCCATGCCGCAGTTCCGCGAGAAGCTCGCCGAC
AGCCGGTTCAACCCCGACCACCCGGTGTGGGTGGAGGACAAGGACTTCGACGTCGACCGGCATCTTCACCGCATCGGGCT
GCCCGCGCCTGGCGGCCGCGAGGAGCTCGCGGAGATCCTCGGCCACATCGCCTCGCTGCCGCTGGACCGCTCCCGGCCGC
TGTGGGAGAAGTGGATCATCGAGGGCATCGACGGGACCGACGCCCGCGACGGGGGACCGCTCGTGGTGCTGACCAAGGTG
CACCACGCCGCCGTCGACGGTGTCACCGGCGCCAGCCTCATGTCGCAGCTGTGCAGCACCGAACCCGATGCGCCGGACCC
CGAACCGGTCGCGGGCGTCGGCGGCGGCAACCAGCTCGAGATCGCCGTCAACGGGGCGATCAAGTTCGCCACCCGGCCGC
TGAAACTGGCCAACGCGTTGCCCGCGACGGTCAACACGGTGCTCGACACCGTCAAGCGTGCGCGTTCGGGGCTGTCGATG
GCGCCTCCCTTCGTGGCGCCGCAGACGGCATGGAACGCCAACGTGACCAGCCACCGCAACATCGCGTTCGCCCAACTCGA
GCTCGACGACGTGAAGGCCGTCAAGAACCACTTCGGCGTCAAGCTCAACGACGTGGTGATGGCGCTGGTCTCGGGCGTGC
TGCGCAAGTTCCTGCAGGATCGGGGGGAACTGCCCACCAGCACGCTGATCGCCGCGGTGCCGGTGTCGGTGCACGACAAG
TCCGACCGGCCCGGACGCAACCAGGTGTCGGCGATGTTCTCCCGCCTCGAGACCCAGATCGAGGACCCGGTCGAACGCCT
CAAGGCCATCTCGGAGGCCAATTCCGTTGCCAAACAGCATAATTCGGCGATCGGCGCCACGCTGCTGCAGGATTGGACGA
CGTTCGCCGCACCCGCGGTGTTCGGGATGGCGATGCGCGTCTACGCCGCCAGCCGGCTGTCCGGCGCCCGCCCGGTGCTC
AACCTCGTGGTCTCCAACGTCCCCGGCCCCCAGATGCCGCTGTACTACCTCGGCAACGAGGTCAAGGCGATGTATCCGAT
CGGACCGGTCTTCCACGGCTCCGGGCTCAACATCACGGTGATGTCGCTCAACGGCAAACTCGACGTGGGGATCGTGTCCT
GCCCCGAGTTGCTGCCCGACCTGTGGGACATGGCCGATGACTTCGGCCACGCACTCGAGGAACTGCTCGACGCGACCCGG
TGA

Upstream 100 bases:

>100_bases
TGTCCCTCGTGATGGTCGGGTCGATCCTCGAGGCCCATCCGGGTCCCCGGGTTCGTCTGACGTCCCAAGTCTGCCACTCC
GGCGCGCGCAGGTGAACACG

Downstream 100 bases:

>100_bases
AACCGCCGCTCGCGGGGGTCGGCGCCGGTTCATGGCAGCATGTGGTGCCATGAGGCTTCGGATCGGGGTCGCGGCGACCG
TCATCGCACTGCCGCTCGTC

Product: diacylglycerol O-acyltransferase

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv1760/MT1809 [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MLSGFAHTVVPDPYAGRMQRLSGLDASFLYLETAQQLLHVCSILELDTTTMPGGYTFAKLRDALSRRIESMPQFREKLAD
SRFNPDHPVWVEDKDFDVDRHLHRIGLPAPGGREELAEILGHIASLPLDRSRPLWEKWIIEGIDGTDARDGGPLVVLTKV
HHAAVDGVTGASLMSQLCSTEPDAPDPEPVAGVGGGNQLEIAVNGAIKFATRPLKLANALPATVNTVLDTVKRARSGLSM
APPFVAPQTAWNANVTSHRNIAFAQLELDDVKAVKNHFGVKLNDVVMALVSGVLRKFLQDRGELPTSTLIAAVPVSVHDK
SDRPGRNQVSAMFSRLETQIEDPVERLKAISEANSVAKQHNSAIGATLLQDWTTFAAPAVFGMAMRVYAASRLSGARPVL
NLVVSNVPGPQMPLYYLGNEVKAMYPIGPVFHGSGLNITVMSLNGKLDVGIVSCPELLPDLWDMADDFGHALEELLDATR

Sequences:

>Translated_480_residues
MLSGFAHTVVPDPYAGRMQRLSGLDASFLYLETAQQLLHVCSILELDTTTMPGGYTFAKLRDALSRRIESMPQFREKLAD
SRFNPDHPVWVEDKDFDVDRHLHRIGLPAPGGREELAEILGHIASLPLDRSRPLWEKWIIEGIDGTDARDGGPLVVLTKV
HHAAVDGVTGASLMSQLCSTEPDAPDPEPVAGVGGGNQLEIAVNGAIKFATRPLKLANALPATVNTVLDTVKRARSGLSM
APPFVAPQTAWNANVTSHRNIAFAQLELDDVKAVKNHFGVKLNDVVMALVSGVLRKFLQDRGELPTSTLIAAVPVSVHDK
SDRPGRNQVSAMFSRLETQIEDPVERLKAISEANSVAKQHNSAIGATLLQDWTTFAAPAVFGMAMRVYAASRLSGARPVL
NLVVSNVPGPQMPLYYLGNEVKAMYPIGPVFHGSGLNITVMSLNGKLDVGIVSCPELLPDLWDMADDFGHALEELLDATR
>Mature_480_residues
MLSGFAHTVVPDPYAGRMQRLSGLDASFLYLETAQQLLHVCSILELDTTTMPGGYTFAKLRDALSRRIESMPQFREKLAD
SRFNPDHPVWVEDKDFDVDRHLHRIGLPAPGGREELAEILGHIASLPLDRSRPLWEKWIIEGIDGTDARDGGPLVVLTKV
HHAAVDGVTGASLMSQLCSTEPDAPDPEPVAGVGGGNQLEIAVNGAIKFATRPLKLANALPATVNTVLDTVKRARSGLSM
APPFVAPQTAWNANVTSHRNIAFAQLELDDVKAVKNHFGVKLNDVVMALVSGVLRKFLQDRGELPTSTLIAAVPVSVHDK
SDRPGRNQVSAMFSRLETQIEDPVERLKAISEANSVAKQHNSAIGATLLQDWTTFAAPAVFGMAMRVYAASRLSGARPVL
NLVVSNVPGPQMPLYYLGNEVKAMYPIGPVFHGSGLNITVMSLNGKLDVGIVSCPELLPDLWDMADDFGHALEELLDATR

Specific function: Upon expression in E.coli functions weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has very weak wax synthase activity, incorporating palmityl alcohol into wax esters in the presence of palmit

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 51970; Mature: 51970

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSGFAHTVVPDPYAGRMQRLSGLDASFLYLETAQQLLHVCSILELDTTTMPGGYTFAKL
CCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
RDALSRRIESMPQFREKLADSRFNPDHPVWVEDKDFDVDRHLHRIGLPAPGGREELAEIL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHH
GHIASLPLDRSRPLWEKWIIEGIDGTDARDGGPLVVLTKVHHAAVDGVTGASLMSQLCST
HHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHCC
EPDAPDPEPVAGVGGGNQLEIAVNGAIKFATRPLKLANALPATVNTVLDTVKRARSGLSM
CCCCCCCCCCEECCCCCEEEEEEECEEEECCCCHHHHHCCHHHHHHHHHHHHHHHCCCCC
APPFVAPQTAWNANVTSHRNIAFAQLELDDVKAVKNHFGVKLNDVVMALVSGVLRKFLQD
CCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RGELPTSTLIAAVPVSVHDKSDRPGRNQVSAMFSRLETQIEDPVERLKAISEANSVAKQH
CCCCCHHHHHEEECEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NSAIGATLLQDWTTFAAPAVFGMAMRVYAASRLSGARPVLNLVVSNVPGPQMPLYYLGNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEECCCC
VKAMYPIGPVFHGSGLNITVMSLNGKLDVGIVSCPELLPDLWDMADDFGHALEELLDATR
CEEEECCCCEEECCCCEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLSGFAHTVVPDPYAGRMQRLSGLDASFLYLETAQQLLHVCSILELDTTTMPGGYTFAKL
CCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
RDALSRRIESMPQFREKLADSRFNPDHPVWVEDKDFDVDRHLHRIGLPAPGGREELAEIL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHH
GHIASLPLDRSRPLWEKWIIEGIDGTDARDGGPLVVLTKVHHAAVDGVTGASLMSQLCST
HHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHCC
EPDAPDPEPVAGVGGGNQLEIAVNGAIKFATRPLKLANALPATVNTVLDTVKRARSGLSM
CCCCCCCCCCEECCCCCEEEEEEECEEEECCCCHHHHHCCHHHHHHHHHHHHHHHCCCCC
APPFVAPQTAWNANVTSHRNIAFAQLELDDVKAVKNHFGVKLNDVVMALVSGVLRKFLQD
CCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RGELPTSTLIAAVPVSVHDKSDRPGRNQVSAMFSRLETQIEDPVERLKAISEANSVAKQH
CCCCCHHHHHEEECEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NSAIGATLLQDWTTFAAPAVFGMAMRVYAASRLSGARPVLNLVVSNVPGPQMPLYYLGNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEECCCC
VKAMYPIGPVFHGSGLNITVMSLNGKLDVGIVSCPELLPDLWDMADDFGHALEELLDATR
CEEEECCCCEEECCCCEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]