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The map label for this gene is murF [H]

Identifier: 108800226

GI number: 108800226

Start: 3455834

End: 3457327

Strand: Reverse

Name: murF [H]

Synonym: Mmcs_3260

Alternate gene names: 108800226

Gene position: 3457327-3455834 (Counterclockwise)

Preceding gene: 108800227

Following gene: 108800225

Centisome position: 60.6

GC content: 72.76

Gene sequence:

>1494_bases
GTGATCGAACTGACCCTGGCCCGGGTGGCCGAGATCGTCGGCGGCCGGCTGGCCGACATCACCCCCGAGGACGCCGCCGC
CACCCGGATCACCGGAACCGTCGAATTCGACTCGCGCGCCGTCACGGCCGGCGGGCTGTTCCTGGCGCTGCCGGGGGCCC
GCTCCGACGGCCACGACTTCGCCGCGGCCGCCGTCGCCGCGGGGGCCGCGGCGGTGCTGGCCGCCCGTCCGGTCGGCGTG
CCCGCCGTCGTCGTCCCTCCTGCCGCGGGGCCCGAGGGCTCCGGTGTGCTCGAACACGACACCGACGGCGCCGGTGCCGC
GGTGCTGGCGGCGCTGGCCCGGTTGGCCGCCGCGGTCGCCGCCGAACTGGTCGACGGCGGGCTGCGGATCGTCGGGATCA
CCGGATCGTCGGGGAAGACCTCGACGAAGGATCTGGTCGCCGCCGTACTCGCCCCGCTGGGGGAGGTGATCGCCCCGCCG
GGTTCGTTCAACAACGAACTCGGTCACCCGTGGACGGTGCTGCGCGCCGACCGCTCGACCGACTTCCTGGTGCTGGAGAT
GTCGGCGCGGCATCCGGGCAACATCGCCGCGCTGGCCGCGATCGCCCCGCCCTCGATCGCGGTGGTGCTCAACGTGGGCA
CCGCCCACCTCGGCGAGTTCGGTTCGCGCGAGGCCATTGCCGCCACGAAATCCGAACTGCCGCAGGCTGTTCCGGAATCC
GGCGTGGTGATCCTCAACGCCGACGACAGTGCGGTCGCCGCGATGGCGGAGGTGACGGCGGCCCGGGTGGTGCGGGTGTC
GCGGGAACCCGGCGCCGACATCTGGGCGGACGCGGTGACGCTCGACGAACTCGCCCGCCCGCGTTTCACCCTGCACGCCG
CGGGCGGTGAGGTGCCGGTGAACCTCGCGGTGCACGGCGACCACCAGGTGTCCAACGCACTGTGTGCGGCCGCGGTCGCG
CTGGAATGCGGGGCCACCCTGGACCAGGTCGCGGCCGCGCTCGCCGGGGCCGGTCCGGTGTCGCGGCACCGTATGCAGGT
CACCACGCGCGAGGACGGCGTCACCGTGGTCAACGACGCCTACAACGCGAACCCGGACTCCATGCGGGCGGGGCTCAAGG
CGCTGGCATGGATGAGTAGGGGCGGGGCGGCCAAACGCCGGAGTTGGGCCGTGCTCGGCGAGATGGCCGAACTCGGTGAC
GACTCGATATCCGAGCACGACAGCATCGGCCGACTGGCGGTGCGCTTAGATGTGTCACGACTCGTCGTCGTAGGAACCGG
GAGGTCGGCAAGCGCCATGCATCAGGGGGCGGTCATGGAGGGATCCTGGGGGGCGGAGGCCACCCGGGTCGATGACGCCG
ACGCTGCGCTGGCGCTGTTGAGCGACGAACTGCAGCCCGGTGATGTCGTGCTGGTGAAGGCGTCGAACTCGGCGGGCCTC
GGTGCGCTGGCCGATTCGCTGACCGCCGGACGTGACGGGACCGCCACCCGATGA

Upstream 100 bases:

>100_bases
TGGTCGCGGGTAAGGGCCACGAGACCGGCCAGACCGCGGCGGGTCGCACCCACCCGTTCGACGACCGCGTCGAACTCGCC
GAACTGCTGGAGAAGCGCGG

Downstream 100 bases:

>100_bases
GGCAGATCCTCATCGCCGTCGGCCTCGCGCTGGCGGTGTCGATCCTGCTGACGCCGGTGCTCATCCGGTTGTTCACCCGG
CAGGGCTTCGGACACGAGAT

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MIELTLARVAEIVGGRLADITPEDAAATRITGTVEFDSRAVTAGGLFLALPGARSDGHDFAAAAVAAGAAAVLAARPVGV
PAVVVPPAAGPEGSGVLEHDTDGAGAAVLAALARLAAAVAAELVDGGLRIVGITGSSGKTSTKDLVAAVLAPLGEVIAPP
GSFNNELGHPWTVLRADRSTDFLVLEMSARHPGNIAALAAIAPPSIAVVLNVGTAHLGEFGSREAIAATKSELPQAVPES
GVVILNADDSAVAAMAEVTAARVVRVSREPGADIWADAVTLDELARPRFTLHAAGGEVPVNLAVHGDHQVSNALCAAAVA
LECGATLDQVAAALAGAGPVSRHRMQVTTREDGVTVVNDAYNANPDSMRAGLKALAWMSRGGAAKRRSWAVLGEMAELGD
DSISEHDSIGRLAVRLDVSRLVVVGTGRSASAMHQGAVMEGSWGAEATRVDDADAALALLSDELQPGDVVLVKASNSAGL
GALADSLTAGRDGTATR

Sequences:

>Translated_497_residues
MIELTLARVAEIVGGRLADITPEDAAATRITGTVEFDSRAVTAGGLFLALPGARSDGHDFAAAAVAAGAAAVLAARPVGV
PAVVVPPAAGPEGSGVLEHDTDGAGAAVLAALARLAAAVAAELVDGGLRIVGITGSSGKTSTKDLVAAVLAPLGEVIAPP
GSFNNELGHPWTVLRADRSTDFLVLEMSARHPGNIAALAAIAPPSIAVVLNVGTAHLGEFGSREAIAATKSELPQAVPES
GVVILNADDSAVAAMAEVTAARVVRVSREPGADIWADAVTLDELARPRFTLHAAGGEVPVNLAVHGDHQVSNALCAAAVA
LECGATLDQVAAALAGAGPVSRHRMQVTTREDGVTVVNDAYNANPDSMRAGLKALAWMSRGGAAKRRSWAVLGEMAELGD
DSISEHDSIGRLAVRLDVSRLVVVGTGRSASAMHQGAVMEGSWGAEATRVDDADAALALLSDELQPGDVVLVKASNSAGL
GALADSLTAGRDGTATR
>Mature_497_residues
MIELTLARVAEIVGGRLADITPEDAAATRITGTVEFDSRAVTAGGLFLALPGARSDGHDFAAAAVAAGAAAVLAARPVGV
PAVVVPPAAGPEGSGVLEHDTDGAGAAVLAALARLAAAVAAELVDGGLRIVGITGSSGKTSTKDLVAAVLAPLGEVIAPP
GSFNNELGHPWTVLRADRSTDFLVLEMSARHPGNIAALAAIAPPSIAVVLNVGTAHLGEFGSREAIAATKSELPQAVPES
GVVILNADDSAVAAMAEVTAARVVRVSREPGADIWADAVTLDELARPRFTLHAAGGEVPVNLAVHGDHQVSNALCAAAVA
LECGATLDQVAAALAGAGPVSRHRMQVTTREDGVTVVNDAYNANPDSMRAGLKALAWMSRGGAAKRRSWAVLGEMAELGD
DSISEHDSIGRLAVRLDVSRLVVVGTGRSASAMHQGAVMEGSWGAEATRVDDADAALALLSDELQPGDVVLVKASNSAGL
GALADSLTAGRDGTATR

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=486, Percent_Identity=34.9794238683128, Blast_Score=181, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49919; Mature: 49919

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELTLARVAEIVGGRLADITPEDAAATRITGTVEFDSRAVTAGGLFLALPGARSDGHDF
CEEEHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCEEEEECCEEEEECCCCCCCCHH
AAAAVAAGAAAVLAARPVGVPAVVVPPAAGPEGSGVLEHDTDGAGAAVLAALARLAAAVA
HHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHH
AELVDGGLRIVGITGSSGKTSTKDLVAAVLAPLGEVIAPPGSFNNELGHPWTVLRADRST
HHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCC
DFLVLEMSARHPGNIAALAAIAPPSIAVVLNVGTAHLGEFGSREAIAATKSELPQAVPES
CEEEEEECCCCCCCEEEEEEECCCCEEEEEECCCHHHCCCCCCCHHHHHHHHHHHHCCCC
GVVILNADDSAVAAMAEVTAARVVRVSREPGADIWADAVTLDELARPRFTLHAAGGEVPV
CEEEEECCCHHHHHHHHHHHHHHEEECCCCCCCHHHHHEEHHHHCCCCEEEEECCCCCEE
NLAVHGDHQVSNALCAAAVALECGATLDQVAAALAGAGPVSRHRMQVTTREDGVTVVNDA
EEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEECC
YNANPDSMRAGLKALAWMSRGGAAKRRSWAVLGEMAELGDDSISEHDSIGRLAVRLDVSR
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEE
LVVVGTGRSASAMHQGAVMEGSWGAEATRVDDADAALALLSDELQPGDVVLVKASNSAGL
EEEEECCCCHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCC
GALADSLTAGRDGTATR
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIELTLARVAEIVGGRLADITPEDAAATRITGTVEFDSRAVTAGGLFLALPGARSDGHDF
CEEEHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCEEEEECCEEEEECCCCCCCCHH
AAAAVAAGAAAVLAARPVGVPAVVVPPAAGPEGSGVLEHDTDGAGAAVLAALARLAAAVA
HHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHH
AELVDGGLRIVGITGSSGKTSTKDLVAAVLAPLGEVIAPPGSFNNELGHPWTVLRADRST
HHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCC
DFLVLEMSARHPGNIAALAAIAPPSIAVVLNVGTAHLGEFGSREAIAATKSELPQAVPES
CEEEEEECCCCCCCEEEEEEECCCCEEEEEECCCHHHCCCCCCCHHHHHHHHHHHHCCCC
GVVILNADDSAVAAMAEVTAARVVRVSREPGADIWADAVTLDELARPRFTLHAAGGEVPV
CEEEEECCCHHHHHHHHHHHHHHEEECCCCCCCHHHHHEEHHHHCCCCEEEEECCCCCEE
NLAVHGDHQVSNALCAAAVALECGATLDQVAAALAGAGPVSRHRMQVTTREDGVTVVNDA
EEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEECC
YNANPDSMRAGLKALAWMSRGGAAKRRSWAVLGEMAELGDDSISEHDSIGRLAVRLDVSR
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEE
LVVVGTGRSASAMHQGAVMEGSWGAEATRVDDADAALALLSDELQPGDVVLVKASNSAGL
EEEEECCCCHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCC
GALADSLTAGRDGTATR
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]