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The map label for this gene is murE [H]

Identifier: 108800227

GI number: 108800227

Start: 3457324

End: 3458850

Strand: Reverse

Name: murE [H]

Synonym: Mmcs_3261

Alternate gene names: 108800227

Gene position: 3458850-3457324 (Counterclockwise)

Preceding gene: 108800228

Following gene: 108800226

Centisome position: 60.62

GC content: 74.66

Gene sequence:

>1527_bases
ATGGCCATGAAGCTGCGCCCCAGCCATCCCGTCGGACACGCGCTCGGTGCCCTTGCCGAGCAGGTGCAGGCGGTTCCCGC
CCCCGGATTCCCGGTGCCCGACATCCGGGTCACCGGGGTGACGTTGCGCGGCCAGGACGCGCTGCCCGGTGACCTGTTCG
CCGCGTTGCCGGGTTCCTCCGCGCACGGGGCGCGGTTCGTCGCCGACGCGGTCGCCGCGGGTGCGGTGGCCGTGCTCACC
GACGCCGCCGGAGCGGTCATGCTGGGTGCGGACCCGGTGGTCCCGGTGCTCGTCCACCCCGATCCCCGCGCAGTGCTCGG
CGCCGTCGCGTCGACCGTCTACGGCCATCCGTCGCAGCACCTGCGCGTCGTCGGCGTGACCGGCACCTCGGGCAAGACCA
CCACCACGTACCTGGTCGAGGCCGGCCTGCGGGCGGCCGGGCGGGTCGCCGGGCTGATCGGCACGGTCGGCATCCGGATC
GACGGGCGCGATCAGCCCAGCGCGCTGACCACCCCCGAGGCACCGGATCTGCAGGCGCTGCTCGCCGTGATGGTCGAACA
GGGCGTCGACACCGTCGTGATGGAGGTGTCCAGCCACGCGCTCACCCTCGGCCGGGTCGACGGGGTGCACTTCGCGGCGG
GCGGGTTCACCAACCTCTCGCGCGACCACCTCGACTTCCACCCGACCATGGAGGACTACTTCGACGCCAAGGCGGGGCTG
TTCGACCCGCAGTCCCGCACCCGCGCCGAGCTCGCGGTGGTGTGTGTCGACGACGACGCGGGCCGGGCGATGGCCATCCG
GGCCGGGCGGGCGGTGACGGTGAGCGCCTCCGGCAGACCCGCGGACTGGCGCGCCGAGGACGTCCGCGCCGTCGAGGCCG
GCGCGCAGGAGTTCGTCGCCGTCGACCCGGCGGGCGTGCACCACGGACTGCGCATCGCCCTGCCCGGGCACTACAACGTC
GCGAACTGTGTGCTGGCCGCCGCGCTGCTCGACGCCGTCGACGTCTCGCCCGAGCAGGCCGCGCCGGGTCTGCGCACCGC
GACGGTGCCCGGCCGGTTGGAGGCCGTCGACCGTGGTCAGGACTTCCTCGCGTTGGTCGACTACGCCCACAAACCGGGCG
CCCTGCGGGCGGTGCTGGAGACGCTGCGGGGCCAGGGAAAGGGCCGCATCGCCGTGGTGTTCGGCGCGGGCGGCAACCGC
GACGCCGGTAAACGCGAACCGATGGGCCGGGTCGCCGCGGAACTGGCGGACCTGGTCGTCGTCACCGACGACAACCCCCG
CGACGAGGACCCCGCGACGATCCGCGCGGCGATCATGGCCGGCGCCCGCGCGGCCGGCGGCGGAGCGGACGTCGTCGAGA
TCGGCGACCGCCGCGAGGCGATCGCCCACGCCGTGCGGTGGGCGCGTCCCGGTGACGTGGTGCTGGTCGCGGGTAAGGGC
CACGAGACCGGCCAGACCGCGGCGGGTCGCACCCACCCGTTCGACGACCGCGTCGAACTCGCCGAACTGCTGGAGAAGCG
CGGGTGA

Upstream 100 bases:

>100_bases
CTGCAGCGGCAGAACGTGCCGCTGTCACCGCCCGGCGCTCCGCTGACACTGCAGGCGACCTGACCGACGCGTCGGGCCGA
CCGAGGCTCGGTACTGTGTC

Downstream 100 bases:

>100_bases
TCGAACTGACCCTGGCCCGGGTGGCCGAGATCGTCGGCGGCCGGCTGGCCGACATCACCCCCGAGGACGCCGCCGCCACC
CGGATCACCGGAACCGTCGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MAMKLRPSHPVGHALGALAEQVQAVPAPGFPVPDIRVTGVTLRGQDALPGDLFAALPGSSAHGARFVADAVAAGAVAVLT
DAAGAVMLGADPVVPVLVHPDPRAVLGAVASTVYGHPSQHLRVVGVTGTSGKTTTTYLVEAGLRAAGRVAGLIGTVGIRI
DGRDQPSALTTPEAPDLQALLAVMVEQGVDTVVMEVSSHALTLGRVDGVHFAAGGFTNLSRDHLDFHPTMEDYFDAKAGL
FDPQSRTRAELAVVCVDDDAGRAMAIRAGRAVTVSASGRPADWRAEDVRAVEAGAQEFVAVDPAGVHHGLRIALPGHYNV
ANCVLAAALLDAVDVSPEQAAPGLRTATVPGRLEAVDRGQDFLALVDYAHKPGALRAVLETLRGQGKGRIAVVFGAGGNR
DAGKREPMGRVAAELADLVVVTDDNPRDEDPATIRAAIMAGARAAGGGADVVEIGDRREAIAHAVRWARPGDVVLVAGKG
HETGQTAAGRTHPFDDRVELAELLEKRG

Sequences:

>Translated_508_residues
MAMKLRPSHPVGHALGALAEQVQAVPAPGFPVPDIRVTGVTLRGQDALPGDLFAALPGSSAHGARFVADAVAAGAVAVLT
DAAGAVMLGADPVVPVLVHPDPRAVLGAVASTVYGHPSQHLRVVGVTGTSGKTTTTYLVEAGLRAAGRVAGLIGTVGIRI
DGRDQPSALTTPEAPDLQALLAVMVEQGVDTVVMEVSSHALTLGRVDGVHFAAGGFTNLSRDHLDFHPTMEDYFDAKAGL
FDPQSRTRAELAVVCVDDDAGRAMAIRAGRAVTVSASGRPADWRAEDVRAVEAGAQEFVAVDPAGVHHGLRIALPGHYNV
ANCVLAAALLDAVDVSPEQAAPGLRTATVPGRLEAVDRGQDFLALVDYAHKPGALRAVLETLRGQGKGRIAVVFGAGGNR
DAGKREPMGRVAAELADLVVVTDDNPRDEDPATIRAAIMAGARAAGGGADVVEIGDRREAIAHAVRWARPGDVVLVAGKG
HETGQTAAGRTHPFDDRVELAELLEKRG
>Mature_507_residues
AMKLRPSHPVGHALGALAEQVQAVPAPGFPVPDIRVTGVTLRGQDALPGDLFAALPGSSAHGARFVADAVAAGAVAVLTD
AAGAVMLGADPVVPVLVHPDPRAVLGAVASTVYGHPSQHLRVVGVTGTSGKTTTTYLVEAGLRAAGRVAGLIGTVGIRID
GRDQPSALTTPEAPDLQALLAVMVEQGVDTVVMEVSSHALTLGRVDGVHFAAGGFTNLSRDHLDFHPTMEDYFDAKAGLF
DPQSRTRAELAVVCVDDDAGRAMAIRAGRAVTVSASGRPADWRAEDVRAVEAGAQEFVAVDPAGVHHGLRIALPGHYNVA
NCVLAAALLDAVDVSPEQAAPGLRTATVPGRLEAVDRGQDFLALVDYAHKPGALRAVLETLRGQGKGRIAVVFGAGGNRD
AGKREPMGRVAAELADLVVVTDDNPRDEDPATIRAAIMAGARAAGGGADVVEIGDRREAIAHAVRWARPGDVVLVAGKGH
ETGQTAAGRTHPFDDRVELAELLEKRG

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=495, Percent_Identity=39.7979797979798, Blast_Score=225, Evalue=7e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 52312; Mature: 52181

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMKLRPSHPVGHALGALAEQVQAVPAPGFPVPDIRVTGVTLRGQDALPGDLFAALPGSS
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCHHEEECCCCC
AHGARFVADAVAAGAVAVLTDAAGAVMLGADPVVPVLVHPDPRAVLGAVASTVYGHPSQH
CCCHHHHHHHHHHCCEEEEECCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCCC
LRVVGVTGTSGKTTTTYLVEAGLRAAGRVAGLIGTVGIRIDGRDQPSALTTPEAPDLQAL
EEEEEEECCCCCCEEEEEEHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCCCCCHHHH
LAVMVEQGVDTVVMEVSSHALTLGRVDGVHFAAGGFTNLSRDHLDFHPTMEDYFDAKAGL
HHHHHHCCCCEEEEEHHCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCC
FDPQSRTRAELAVVCVDDDAGRAMAIRAGRAVTVSASGRPADWRAEDVRAVEAGAQEFVA
CCCCCCCCEEEEEEEEECCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHHHCCCHHEEE
VDPAGVHHGLRIALPGHYNVANCVLAAALLDAVDVSPEQAAPGLRTATVPGRLEAVDRGQ
ECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCHHHCCCCCEEECCCCCHHHHHCCC
DFLALVDYAHKPGALRAVLETLRGQGKGRIAVVFGAGGNRDAGKREPMGRVAAELADLVV
CEEEEHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCEEE
VTDDNPRDEDPATIRAAIMAGARAAGGGADVVEIGDRREAIAHAVRWARPGDVVLVAGKG
EECCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCEEEEECCC
HETGQTAAGRTHPFDDRVELAELLEKRG
CCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AMKLRPSHPVGHALGALAEQVQAVPAPGFPVPDIRVTGVTLRGQDALPGDLFAALPGSS
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCCCCCHHEEECCCCC
AHGARFVADAVAAGAVAVLTDAAGAVMLGADPVVPVLVHPDPRAVLGAVASTVYGHPSQH
CCCHHHHHHHHHHCCEEEEECCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCCC
LRVVGVTGTSGKTTTTYLVEAGLRAAGRVAGLIGTVGIRIDGRDQPSALTTPEAPDLQAL
EEEEEEECCCCCCEEEEEEHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCCCCCHHHH
LAVMVEQGVDTVVMEVSSHALTLGRVDGVHFAAGGFTNLSRDHLDFHPTMEDYFDAKAGL
HHHHHHCCCCEEEEEHHCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCC
FDPQSRTRAELAVVCVDDDAGRAMAIRAGRAVTVSASGRPADWRAEDVRAVEAGAQEFVA
CCCCCCCCEEEEEEEEECCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHHHCCCHHEEE
VDPAGVHHGLRIALPGHYNVANCVLAAALLDAVDVSPEQAAPGLRTATVPGRLEAVDRGQ
ECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCHHHCCCCCEEECCCCCHHHHHCCC
DFLALVDYAHKPGALRAVLETLRGQGKGRIAVVFGAGGNRDAGKREPMGRVAAELADLVV
CEEEEHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCEEE
VTDDNPRDEDPATIRAAIMAGARAAGGGADVVEIGDRREAIAHAVRWARPGDVVLVAGKG
EECCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCEEEEECCC
HETGQTAAGRTHPFDDRVELAELLEKRG
CCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA