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The map label for this gene is murD [H]

Identifier: 108800224

GI number: 108800224

Start: 3453269

End: 3454756

Strand: Reverse

Name: murD [H]

Synonym: Mmcs_3258

Alternate gene names: 108800224

Gene position: 3454756-3453269 (Counterclockwise)

Preceding gene: 108800225

Following gene: 108800223

Centisome position: 60.55

GC content: 73.72

Gene sequence:

>1488_bases
GTGGCCCGCGCAGTCGTCGAACCGCTGACCCCGGGCGTCCGGGTGCTGATCACCGGCGCCGGGCTGACCGGGCGGTCGGT
CAGCGCGGTGCTCGAGCCGACCGGTGTGCGGTTGACCATCTGCGACGACGACCCGCTGGCGCTGCAGCGGCTGATCACCC
CCGCCGCGGTCGTCACCACCGGTGAGGCGGTGGCCGGGATCGCCGACTACGCCCTTGTCGTCACCAGCCCCGGATTCCCC
CCGACCGCACCGGTGCTGGCCGCGGCCGCGGCCGCCGGGGTGCCGATCTGGGGTGACGTGGAGCTGGCGTGGCGGCTGGA
CCGGGCGGGCTGGTTCGGGCCGCCGCGGCGTTGGCTGGTGGTCACCGGCACCAACGGCAAGACGACCACCACCTCGATGC
TCTACGCGATCCTGCGCGCCGCGGACCGCCGGGCGATGCTGTGCGGCAACATCGGCAACCCTGTCCTCGACGTGCTCGGT
GAGTCACCGCGGACGCGAGGAGATGAGGAACCCGAACTGCTGGCGGTCGAGCTCTCGAGCTTCCAGCTCCATTGGGCGCC
GTCGCTGCGCCCGGACGCGGGTGTGGTGCTCAACGTCGCCGAGGACCATCTGGACTGGCACGGGTCGATGACGGCCTACG
CCCGCGACAAGGCCAGGGCGCTCGACGGCCGGGTGGCCGTGGCAGGTCTCGACGACCCGGTGGCGGCCGAGCTGCTCACG
ACCGCCGCGGCGCCGGTGCGGGTGGGCTTCCGGCTGGGTCCGCCGGGGGCCGGTGAACTGGGCGTCCACGACGGGTGGCT
GGTCGACAACGCGTTCGCCGACGGCCTTCGGCTGGCGCGCGCCGACGACATCCGGGTGGCCGGACCGGTCGGGGTGCTCA
ACGCGCTGGCGGCGGCCGCGCTTGCCCGCGCGGTCGACGTACCGGCCGAGGCGGTGGCCGCGGCGCTGGCCTCGTTCGAG
GTGGGCCGGCACCGCGCCGAAGTGGTCGCGGTCTCCGGCGGCGTCACCTGGGTCGACGACTCGAAGGCCACCAATCCGCA
TGCCGCCCGCGCCTCGATCGCGGCGTATCCGCGGGTGGTGTGGATCGCCGGCGGACTGCTCAAGGGCGTGTCGGTCGACG
CGCTCGTCGCCGAGGTCGCCGACCGGCTCGCCGGGGTGGTGTTGATCGGCCGCGACCGTGCGGTGGTCGCCGAGGCGTTA
TCGCGACACGCGCCGGATGTCCCCGTCGTCGAGCTTGTGACGGGGGAGGATTCTGGGGTGCATGGGACAACTGAGTCAGC
TGTGAATCATGTGACTCGTGTGATCGACACGGGAGGGCGGCCGCTACCCGATGCGGTGATGCGGGCGGCGGTCGACGCCG
CGCGCCACCTCGCCCGCCCCGGTGACGCTGTGCTGCTGGCGCCCGCAGGCGCCTCATTCGACCAGTTCAGCGGCTACACC
CAGCGCGGCGACGCCTTCGCCGCCGCGGTGCGCGCCGATCTCGGGTAG

Upstream 100 bases:

>100_bases
ACCACGGTGATCATCCGGTTCTGGTTGCTCACCGCGATCGCCTGCGGGCTGGGCGTGGCGCTGTTCTACGGCGAGTGGCT
CACCGCCGTCGGGGCTTGAC

Downstream 100 bases:

>100_bases
ACGCCGTGGCGACGATCCTGGCCCGGTTGCGCGGTAAGGCGGCCGGCCCCGGCGCCGACGAACCGCCGAGGGCCGCCCAA
CCCCCCACGGTGTCCCACAC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MARAVVEPLTPGVRVLITGAGLTGRSVSAVLEPTGVRLTICDDDPLALQRLITPAAVVTTGEAVAGIADYALVVTSPGFP
PTAPVLAAAAAAGVPIWGDVELAWRLDRAGWFGPPRRWLVVTGTNGKTTTTSMLYAILRAADRRAMLCGNIGNPVLDVLG
ESPRTRGDEEPELLAVELSSFQLHWAPSLRPDAGVVLNVAEDHLDWHGSMTAYARDKARALDGRVAVAGLDDPVAAELLT
TAAAPVRVGFRLGPPGAGELGVHDGWLVDNAFADGLRLARADDIRVAGPVGVLNALAAAALARAVDVPAEAVAAALASFE
VGRHRAEVVAVSGGVTWVDDSKATNPHAARASIAAYPRVVWIAGGLLKGVSVDALVAEVADRLAGVVLIGRDRAVVAEAL
SRHAPDVPVVELVTGEDSGVHGTTESAVNHVTRVIDTGGRPLPDAVMRAAVDAARHLARPGDAVLLAPAGASFDQFSGYT
QRGDAFAAAVRADLG

Sequences:

>Translated_495_residues
MARAVVEPLTPGVRVLITGAGLTGRSVSAVLEPTGVRLTICDDDPLALQRLITPAAVVTTGEAVAGIADYALVVTSPGFP
PTAPVLAAAAAAGVPIWGDVELAWRLDRAGWFGPPRRWLVVTGTNGKTTTTSMLYAILRAADRRAMLCGNIGNPVLDVLG
ESPRTRGDEEPELLAVELSSFQLHWAPSLRPDAGVVLNVAEDHLDWHGSMTAYARDKARALDGRVAVAGLDDPVAAELLT
TAAAPVRVGFRLGPPGAGELGVHDGWLVDNAFADGLRLARADDIRVAGPVGVLNALAAAALARAVDVPAEAVAAALASFE
VGRHRAEVVAVSGGVTWVDDSKATNPHAARASIAAYPRVVWIAGGLLKGVSVDALVAEVADRLAGVVLIGRDRAVVAEAL
SRHAPDVPVVELVTGEDSGVHGTTESAVNHVTRVIDTGGRPLPDAVMRAAVDAARHLARPGDAVLLAPAGASFDQFSGYT
QRGDAFAAAVRADLG
>Mature_494_residues
ARAVVEPLTPGVRVLITGAGLTGRSVSAVLEPTGVRLTICDDDPLALQRLITPAAVVTTGEAVAGIADYALVVTSPGFPP
TAPVLAAAAAAGVPIWGDVELAWRLDRAGWFGPPRRWLVVTGTNGKTTTTSMLYAILRAADRRAMLCGNIGNPVLDVLGE
SPRTRGDEEPELLAVELSSFQLHWAPSLRPDAGVVLNVAEDHLDWHGSMTAYARDKARALDGRVAVAGLDDPVAAELLTT
AAAPVRVGFRLGPPGAGELGVHDGWLVDNAFADGLRLARADDIRVAGPVGVLNALAAAALARAVDVPAEAVAAALASFEV
GRHRAEVVAVSGGVTWVDDSKATNPHAARASIAAYPRVVWIAGGLLKGVSVDALVAEVADRLAGVVLIGRDRAVVAEALS
RHAPDVPVVELVTGEDSGVHGTTESAVNHVTRVIDTGGRPLPDAVMRAAVDAARHLARPGDAVLLAPAGASFDQFSGYTQ
RGDAFAAAVRADLG

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=489, Percent_Identity=31.0838445807771, Blast_Score=144, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 50943; Mature: 50812

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARAVVEPLTPGVRVLITGAGLTGRSVSAVLEPTGVRLTICDDDPLALQRLITPAAVVTT
CCCCCCCCCCCCEEEEEEECCCCCCCHHEEECCCCCEEEEECCCHHHHHHHHCCHHEEEC
GEAVAGIADYALVVTSPGFPPTAPVLAAAAAAGVPIWGDVELAWRLDRAGWFGPPRRWLV
CHHHHHHHCEEEEEECCCCCCCHHHHHHHHHCCCCEECCEEEEEEECCCCCCCCCCEEEE
VTGTNGKTTTTSMLYAILRAADRRAMLCGNIGNPVLDVLGESPRTRGDEEPELLAVELSS
EECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEC
FQLHWAPSLRPDAGVVLNVAEDHLDWHGSMTAYARDKARALDGRVAVAGLDDPVAAELLT
EEEEECCCCCCCCCEEEEEHHHCCCCCCCEEEHHHHHHHHCCCEEEEECCCCHHHHHHHH
TAAAPVRVGFRLGPPGAGELGVHDGWLVDNAFADGLRLARADDIRVAGPVGVLNALAAAA
HCCCCEEEEEEECCCCCCCCCCCCCEEEHHHHHCCEEEECCCCEEEECHHHHHHHHHHHH
LARAVDVPAEAVAAALASFEVGRHRAEVVAVSGGVTWVDDSKATNPHAARASIAAYPRVV
HHHHHCCCHHHHHHHHHHHHHCCCEEEEEEEECCEEEECCCCCCCCCHHHHHHHHCCEEE
WIAGGLLKGVSVDALVAEVADRLAGVVLIGRDRAVVAEALSRHAPDVPVVELVTGEDSGV
EEECHHHCCCCHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCCCC
HGTTESAVNHVTRVIDTGGRPLPDAVMRAAVDAARHLARPGDAVLLAPAGASFDQFSGYT
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCC
QRGDAFAAAVRADLG
CCCCHHHHHHHHCCC
>Mature Secondary Structure 
ARAVVEPLTPGVRVLITGAGLTGRSVSAVLEPTGVRLTICDDDPLALQRLITPAAVVTT
CCCCCCCCCCCEEEEEEECCCCCCCHHEEECCCCCEEEEECCCHHHHHHHHCCHHEEEC
GEAVAGIADYALVVTSPGFPPTAPVLAAAAAAGVPIWGDVELAWRLDRAGWFGPPRRWLV
CHHHHHHHCEEEEEECCCCCCCHHHHHHHHHCCCCEECCEEEEEEECCCCCCCCCCEEEE
VTGTNGKTTTTSMLYAILRAADRRAMLCGNIGNPVLDVLGESPRTRGDEEPELLAVELSS
EECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEC
FQLHWAPSLRPDAGVVLNVAEDHLDWHGSMTAYARDKARALDGRVAVAGLDDPVAAELLT
EEEEECCCCCCCCCEEEEEHHHCCCCCCCEEEHHHHHHHHCCCEEEEECCCCHHHHHHHH
TAAAPVRVGFRLGPPGAGELGVHDGWLVDNAFADGLRLARADDIRVAGPVGVLNALAAAA
HCCCCEEEEEEECCCCCCCCCCCCCEEEHHHHHCCEEEECCCCEEEECHHHHHHHHHHHH
LARAVDVPAEAVAAALASFEVGRHRAEVVAVSGGVTWVDDSKATNPHAARASIAAYPRVV
HHHHHCCCHHHHHHHHHHHHHCCCEEEEEEEECCEEEECCCCCCCCCHHHHHHHHCCEEE
WIAGGLLKGVSVDALVAEVADRLAGVVLIGRDRAVVAEALSRHAPDVPVVELVTGEDSGV
EEECHHHCCCCHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCCCC
HGTTESAVNHVTRVIDTGGRPLPDAVMRAAVDAARHLARPGDAVLLAPAGASFDQFSGYT
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCC
QRGDAFAAAVRADLG
CCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA