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The map label for this gene is braC [H]

Identifier: 108799986

GI number: 108799986

Start: 3191216

End: 3192427

Strand: Reverse

Name: braC [H]

Synonym: Mmcs_3020

Alternate gene names: 108799986

Gene position: 3192427-3191216 (Counterclockwise)

Preceding gene: 108799987

Following gene: 108799985

Centisome position: 55.95

GC content: 67.49

Gene sequence:

>1212_bases
GTGCGCGGTCGCGTGGCACGGAACGCATTCGCTTTCGGAAGTGCGGGGCTGTTGGCGCTCGCGCTCGGCGCCTGCAGTCA
ATCGACACCCGAAGAGGAAGCGGCGCAGACGAATCTGAAGATCGTCGAGCAGGTCCAGATCGACGAGAACGGCGCGGAGG
TCAAGGCCGCCGAAGGAGCCGCCCCGGCGGATCCCGCCGGTGACGGCAAGGCGACCTGCCCACCGCTGTCGATCGCGATG
GCCGGGGCGCTCAACGGCCCGGACGCGGCCCTGGGCATCAACATCAAGAACGGTGTGCAGCTCGCCGTCGACAAGCACAA
CGCGGCCAACCCCGGCTGCCAGGTCCAGCTCAAGACGTTCGACACCGAGGGTGACCCGCAGAAGGCCAGCGCGATCGCCC
CGCAGATCGTCGACGACCAGTACACGATCGGCCTTGTCGGCCCGGCCTTCTCGGGTGAGACCAACGCCACCGGCGACGTG
TTCAACCAGGCCGGCCTGGTCGCGGCCACCGCCTCGGCCACCAACGTCACGCTGTCGGAGAAGGGGTGGCGGACGTTCTT
CCGCGGCCTGGCCAACGACGGTGTGCAGGGCCCGTCGGTCGCCAACTACCTGAAGAACACGCTCGGTCACCAGAAGGTGT
GCGTCGTCGACGACAGCACCGACTACGGTTTGGGCCTGGCTCAGGCGGTCCGCGAAACCCTCGGGCCGGTCGCCGATCCG
GCGTGCAACATCTCGGTGAAGAAGGGCGACAAGGACTTCTCCGCCGCCGTCACCCAGGTCAAGGGGGCGAGCCCGCAGTC
GGTGTTCTTCAGCGGCTACTACGCCGAGGCGGCGCCGTTCGTCCAGCAGCTCAAGGACGGCGGTTTCGAGGGCACGTTCG
TCAGCGCCGACGGCACCAAGGATCCGGAGTTCGTCAAGCAGGCCGGTGAGGCGTCCAAGGACGCGCTGCTGTCGTGCCCG
TGCGGCCCGGCCACCGGCGGCTTCGCCGACGAGTACAACCAGAAGTTCGGCCAGGAGCCCGGCACCTACAGCACCGAGGG
ATACGACCTGGGCACCATCCTGGTCAAGGGCATCGATTCCGGGGCCATCACCCGCCAGGCGCTGCTGGACTACGTGCGCA
ACTACAACGGCCAGGGTGTGGCGCGCAACTATCAGTGGACACCGCAGGGTGAGCTCACCACCACCCTGATCTGGATGTAC
AAGGTCCAGTGA

Upstream 100 bases:

>100_bases
GGCCAGGTGTTGGCTATGTTCTACCGGAAGCGTCAGCGCCCTGCGGCCGATCCGGTCGCGGGAGTCGATGCCGATGACAA
CATCGACCAGGAGGTGAAAC

Downstream 100 bases:

>100_bases
TCGACTAGGCGAGACGCGTCCGAACCAGTTGGCTCGGACGCGTTTCGTTTAGCACCCCCACCGCGCGAGGAGCCCACCGC
ACCGATGATCGCCGAGTGCG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MRGRVARNAFAFGSAGLLALALGACSQSTPEEEAAQTNLKIVEQVQIDENGAEVKAAEGAAPADPAGDGKATCPPLSIAM
AGALNGPDAALGINIKNGVQLAVDKHNAANPGCQVQLKTFDTEGDPQKASAIAPQIVDDQYTIGLVGPAFSGETNATGDV
FNQAGLVAATASATNVTLSEKGWRTFFRGLANDGVQGPSVANYLKNTLGHQKVCVVDDSTDYGLGLAQAVRETLGPVADP
ACNISVKKGDKDFSAAVTQVKGASPQSVFFSGYYAEAAPFVQQLKDGGFEGTFVSADGTKDPEFVKQAGEASKDALLSCP
CGPATGGFADEYNQKFGQEPGTYSTEGYDLGTILVKGIDSGAITRQALLDYVRNYNGQGVARNYQWTPQGELTTTLIWMY
KVQ

Sequences:

>Translated_403_residues
MRGRVARNAFAFGSAGLLALALGACSQSTPEEEAAQTNLKIVEQVQIDENGAEVKAAEGAAPADPAGDGKATCPPLSIAM
AGALNGPDAALGINIKNGVQLAVDKHNAANPGCQVQLKTFDTEGDPQKASAIAPQIVDDQYTIGLVGPAFSGETNATGDV
FNQAGLVAATASATNVTLSEKGWRTFFRGLANDGVQGPSVANYLKNTLGHQKVCVVDDSTDYGLGLAQAVRETLGPVADP
ACNISVKKGDKDFSAAVTQVKGASPQSVFFSGYYAEAAPFVQQLKDGGFEGTFVSADGTKDPEFVKQAGEASKDALLSCP
CGPATGGFADEYNQKFGQEPGTYSTEGYDLGTILVKGIDSGAITRQALLDYVRNYNGQGVARNYQWTPQGELTTTLIWMY
KVQ
>Mature_403_residues
MRGRVARNAFAFGSAGLLALALGACSQSTPEEEAAQTNLKIVEQVQIDENGAEVKAAEGAAPADPAGDGKATCPPLSIAM
AGALNGPDAALGINIKNGVQLAVDKHNAANPGCQVQLKTFDTEGDPQKASAIAPQIVDDQYTIGLVGPAFSGETNATGDV
FNQAGLVAATASATNVTLSEKGWRTFFRGLANDGVQGPSVANYLKNTLGHQKVCVVDDSTDYGLGLAQAVRETLGPVADP
ACNISVKKGDKDFSAAVTQVKGASPQSVFFSGYYAEAAPFVQQLKDGGFEGTFVSADGTKDPEFVKQAGEASKDALLSCP
CGPATGGFADEYNQKFGQEPGTYSTEGYDLGTILVKGIDSGAITRQALLDYVRNYNGQGVARNYQWTPQGELTTTLIWMY
KVQ

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=269, Percent_Identity=28.996282527881, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI48994941, Length=255, Percent_Identity=29.0196078431373, Blast_Score=111, Evalue=7e-26,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41896; Mature: 41896

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGRVARNAFAFGSAGLLALALGACSQSTPEEEAAQTNLKIVEQVQIDENGAEVKAAEGA
CCCCCCCCHHHCCCHHHHHHHHHHCCCCCCHHHHHHCCCEEEEEEEECCCCCEEEECCCC
APADPAGDGKATCPPLSIAMAGALNGPDAALGINIKNGVQLAVDKHNAANPGCQVQLKTF
CCCCCCCCCCCCCCCHHEEEECCCCCCCCEEEEEECCCEEEEEECCCCCCCCCEEEEEEE
DTEGDPQKASAIAPQIVDDQYTIGLVGPAFSGETNATGDVFNQAGLVAATASATNVTLSE
CCCCCCHHHHHCCCHHCCCCEEEEEECCCCCCCCCCCHHHHHCCCEEEEECCCCEEEECC
KGWRTFFRGLANDGVQGPSVANYLKNTLGHQKVCVVDDSTDYGLGLAQAVRETLGPVADP
HHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCC
ACNISVKKGDKDFSAAVTQVKGASPQSVFFSGYYAEAAPFVQQLKDGGFEGTFVSADGTK
CCEEEEECCCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEECCCCC
DPEFVKQAGEASKDALLSCPCGPATGGFADEYNQKFGQEPGTYSTEGYDLGTILVKGIDS
CHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCC
GAITRQALLDYVRNYNGQGVARNYQWTPQGELTTTLIWMYKVQ
CHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCEEEEEEEEEEC
>Mature Secondary Structure
MRGRVARNAFAFGSAGLLALALGACSQSTPEEEAAQTNLKIVEQVQIDENGAEVKAAEGA
CCCCCCCCHHHCCCHHHHHHHHHHCCCCCCHHHHHHCCCEEEEEEEECCCCCEEEECCCC
APADPAGDGKATCPPLSIAMAGALNGPDAALGINIKNGVQLAVDKHNAANPGCQVQLKTF
CCCCCCCCCCCCCCCHHEEEECCCCCCCCEEEEEECCCEEEEEECCCCCCCCCEEEEEEE
DTEGDPQKASAIAPQIVDDQYTIGLVGPAFSGETNATGDVFNQAGLVAATASATNVTLSE
CCCCCCHHHHHCCCHHCCCCEEEEEECCCCCCCCCCCHHHHHCCCEEEEECCCCEEEECC
KGWRTFFRGLANDGVQGPSVANYLKNTLGHQKVCVVDDSTDYGLGLAQAVRETLGPVADP
HHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCC
ACNISVKKGDKDFSAAVTQVKGASPQSVFFSGYYAEAAPFVQQLKDGGFEGTFVSADGTK
CCEEEEECCCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEECCCCC
DPEFVKQAGEASKDALLSCPCGPATGGFADEYNQKFGQEPGTYSTEGYDLGTILVKGIDS
CHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCC
GAITRQALLDYVRNYNGQGVARNYQWTPQGELTTTLIWMYKVQ
CHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]