Click here to switch to the map view.

The map label for this gene is argF [H]

Identifier: 108799934

GI number: 108799934

Start: 3132621

End: 3133544

Strand: Reverse

Name: argF [H]

Synonym: Mmcs_2968

Alternate gene names: 108799934

Gene position: 3133544-3132621 (Counterclockwise)

Preceding gene: 108799935

Following gene: 108799933

Centisome position: 54.92

GC content: 70.24

Gene sequence:

>924_bases
ATGAGACATTTCCTGCGCGACGACGACCTGACCCCCGCCGAGCAGGCCGAGGTGCTGGCCCTGGCCGCCGAGCTGAAGAA
GGATCCGCTGCTGCACCGCCCGCTGGCCGGGCCGCGCGGTGTCGCGGTGATCTTCGACAAGAACTCGACGCGCACGCGGT
TCTCCTTCGAGGTGGGCATCGCCCAGCTCGGCGGGCACGCCGTCGTGGTGGACGGTCGCAGCACCCAGCTCGGCCGCGAG
GAGACCCTCGAGGACACCGGAAAGGTGTTGTCCCGCTACGTCGATGCGATCGTGTGGCGCACCTTCGCCCAGGAACGGCT
GACCTCCATGGCCGCCGGGGCGACCGTGCCGGTGATCAACGCGCTCTCCGACGAGTTCCACCCCTGTCAGGTCCTTGCCG
ACCTGCAGACCCTGATCGAACGCCGCGGCGCGCTACCCGGCCTGCGGATGGCCTACTTCGGTGACGGTGCCAACAACATG
GCGCACTCGCTGATGCTCGGCGGCGCCACCGCCGGCATGCACGTGACGATCGCGGCACCGCAGGGCTTCGAACCGCATCC
GATGTTCGTCGCCGCCGCGCAGGAGCGCGCGGCGCAGACCGGCGCGTCGGTCACCGTCACCGCCGACCCGCATCGGGCCG
CCGACGGTGCGGACGTCCTCGTCACCGACACGTGGACGTCGATGGGTCAGGAGAACGACGGCCTCGACCGGGTGCGACCG
TTCCGGCCGTTTCAGGTCAACACCGGCCTGCTCGACCGCGCCCATGCCGAAGCCGTTGTGCTGCACTGCCTTCCGGCCCA
CCGCGGGCACGAAATCACCGACGAGGTCATCGACGGCCCGCACAGCGCGGTGTGGGACGAAGCGGAGAACCGGCTGCACG
CGCAGAAGGCGCTGCTGGTGTGGCTGCTGGAGCGGTCGGGGTGA

Upstream 100 bases:

>100_bases
AAGCCCAGATCGACGACTTCCTCGGTGCGCTCCCCGGCGTACTCGACGCCGCCGCCGCCGGAGGCGGCGAGTCAACCCCG
GCTGCGGAGGTGGCCGACAC

Downstream 100 bases:

>100_bases
CGGTGTCATGACTTCCGCGGTGACCAGAGCCGGACGACAGGCCCGTATCGTGGCGATCCTGTCGTCGCATTCCGTGCGCA
GCCAGGGTGAGCTGGCCGCC

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MRHFLRDDDLTPAEQAEVLALAAELKKDPLLHRPLAGPRGVAVIFDKNSTRTRFSFEVGIAQLGGHAVVVDGRSTQLGRE
ETLEDTGKVLSRYVDAIVWRTFAQERLTSMAAGATVPVINALSDEFHPCQVLADLQTLIERRGALPGLRMAYFGDGANNM
AHSLMLGGATAGMHVTIAAPQGFEPHPMFVAAAQERAAQTGASVTVTADPHRAADGADVLVTDTWTSMGQENDGLDRVRP
FRPFQVNTGLLDRAHAEAVVLHCLPAHRGHEITDEVIDGPHSAVWDEAENRLHAQKALLVWLLERSG

Sequences:

>Translated_307_residues
MRHFLRDDDLTPAEQAEVLALAAELKKDPLLHRPLAGPRGVAVIFDKNSTRTRFSFEVGIAQLGGHAVVVDGRSTQLGRE
ETLEDTGKVLSRYVDAIVWRTFAQERLTSMAAGATVPVINALSDEFHPCQVLADLQTLIERRGALPGLRMAYFGDGANNM
AHSLMLGGATAGMHVTIAAPQGFEPHPMFVAAAQERAAQTGASVTVTADPHRAADGADVLVTDTWTSMGQENDGLDRVRP
FRPFQVNTGLLDRAHAEAVVLHCLPAHRGHEITDEVIDGPHSAVWDEAENRLHAQKALLVWLLERSG
>Mature_307_residues
MRHFLRDDDLTPAEQAEVLALAAELKKDPLLHRPLAGPRGVAVIFDKNSTRTRFSFEVGIAQLGGHAVVVDGRSTQLGRE
ETLEDTGKVLSRYVDAIVWRTFAQERLTSMAAGATVPVINALSDEFHPCQVLADLQTLIERRGALPGLRMAYFGDGANNM
AHSLMLGGATAGMHVTIAAPQGFEPHPMFVAAAQERAAQTGASVTVTADPHRAADGADVLVTDTWTSMGQENDGLDRVRP
FRPFQVNTGLLDRAHAEAVVLHCLPAHRGHEITDEVIDGPHSAVWDEAENRLHAQKALLVWLLERSG

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=305, Percent_Identity=40.9836065573771, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=27.0096463022508, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1790703, Length=331, Percent_Identity=37.7643504531722, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1786469, Length=326, Percent_Identity=36.8098159509202, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI2367364, Length=313, Percent_Identity=33.2268370607029, Blast_Score=97, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6322373, Length=326, Percent_Identity=34.0490797546012, Blast_Score=184, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6322331, Length=310, Percent_Identity=26.4516129032258, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24642586, Length=312, Percent_Identity=27.8846153846154, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 33276; Mature: 33276

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHFLRDDDLTPAEQAEVLALAAELKKDPLLHRPLAGPRGVAVIFDKNSTRTRFSFEVGI
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECH
AQLGGHAVVVDGRSTQLGREETLEDTGKVLSRYVDAIVWRTFAQERLTSMAAGATVPVIN
HHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ALSDEFHPCQVLADLQTLIERRGALPGLRMAYFGDGANNMAHSLMLGGATAGMHVTIAAP
HHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCCCCEEEEEECC
QGFEPHPMFVAAAQERAAQTGASVTVTADPHRAADGADVLVTDTWTSMGQENDGLDRVRP
CCCCCCCEEEEHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEECHHHCCCCCCCHHHCCC
FRPFQVNTGLLDRAHAEAVVLHCLPAHRGHEITDEVIDGPHSAVWDEAENRLHAQKALLV
CCCEEECCCCHHHHHHHEEEEEECCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHH
WLLERSG
HEEECCC
>Mature Secondary Structure
MRHFLRDDDLTPAEQAEVLALAAELKKDPLLHRPLAGPRGVAVIFDKNSTRTRFSFEVGI
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECH
AQLGGHAVVVDGRSTQLGREETLEDTGKVLSRYVDAIVWRTFAQERLTSMAAGATVPVIN
HHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ALSDEFHPCQVLADLQTLIERRGALPGLRMAYFGDGANNMAHSLMLGGATAGMHVTIAAP
HHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCCCCEEEEEECC
QGFEPHPMFVAAAQERAAQTGASVTVTADPHRAADGADVLVTDTWTSMGQENDGLDRVRP
CCCCCCCEEEEHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEECHHHCCCCCCCHHHCCC
FRPFQVNTGLLDRAHAEAVVLHCLPAHRGHEITDEVIDGPHSAVWDEAENRLHAQKALLV
CCCEEECCCCHHHHHHHEEEEEECCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHH
WLLERSG
HEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA