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The map label for this gene is tyrS [H]

Identifier: 108799921

GI number: 108799921

Start: 3118900

End: 3120171

Strand: Reverse

Name: tyrS [H]

Synonym: Mmcs_2955

Alternate gene names: 108799921

Gene position: 3120171-3118900 (Counterclockwise)

Preceding gene: 108799922

Following gene: 108799920

Centisome position: 54.69

GC content: 68.08

Gene sequence:

>1272_bases
ATGAGCAGCATTCTCGACGAGTTGGACTGGCGCGGGCTGATCGCGCAATCGACCGACCGGGACGCACTGGCGGCCGAACT
GGCCGCCGGTCCGATGACGCTGTACTCCGGCTTCGACCCCACCGCGCCCAGCCTGCATGCCGGACATCTCGTGCCACTGC
TGACGCTGCGCCGTTTTCAGCAGGCCGGTCACCGGCCGATCGTGCTGGCCGGCGGGGCCACCGGAATGATCGGCGACCCG
CGGGACACGGGGGAGCGCACACTGCAGACCGCCGACACGGTCGCCGACTGGGCGGACCGGATCCGGGGTCAGCTCGAGCG
GTTCGTGGAGTTCGACGAATCGCCCACCGGCGCCATCGTCGAGAACAACCTCTCCTGGACCGGCGCGTTGTCGACGATCG
AATTCCTCCGTGACGTGGGCAAGTACTTCTCGGTGAATGTGATGCTCGACCGCGACACCGTCCGGCGGCGCCTGGAGGGA
GAAGGGATCTCCTACACCGAGTTCAGCTACATGCTGTTGCAGGCCAACGACTATGTGCAGTTGCGGAAACGCCACGGCTG
CGCGCTGCAGATCGGCGGTTCCGATCAGTGGGGCAACATCGTGGCCGGGGTCCGGTTGGTGCGCCAGAAACTCGGCGACA
CCGTCCACGCGATGACGACCCCGCTGGTCACCGACTCGGAGGGCAAGAAGTTCGGGAAGTCGACCGGGGGTGGGAACCTG
TGGCTCGACCCGGAGATGACCACGCCGTACGCCTGGTACCAGTACTTCATCAACACTGCCGACGCTGACGTCGTCAACTA
CCTGCGGTGGTTCACGTTCCTCGAGGCCGGCGAGTTGGCGGAGCTCGAGGAGGCGACCCGTGATCGACCTCACCAGCGCA
CCGCCCAGCGGCGCCTCGCCCGCGAGCTGACCACGCTGGTCCACGGCGAGGATGCGACCCGGTCGGTGGAGCACGCCAGC
CAGGCGCTGTTCGGCCGCGGTGAGCTCGCCGCGCTCGACGAACCGACCCTGGCCGCGGCGCTGCGTGAGGCGTCGGTGGC
CGAACTCACCCCGAGCGGGCCGGATCTCATCACCGATCTGCTGGTCGCCACCGGCCTGTCGGCGAGTAAGGGCGCCGCGC
GCAGGACCATCGCCGAGGGCGGGGTCTCGGTGAACAACATGAAGATCGACAGCGACGAGTGGACCCCGCAGGCCTCGGAC
TTCCTGCACGGCAGGTGGTTGGTCCTGCGCCGCGGCAAGCGGAACATCGCCGGCGTGCAGCGCGTTGGATGA

Upstream 100 bases:

>100_bases
GTTGGCCGGCCGGCCGGAGGTGTCGGCGTACCGGCGCAGCCCGCGAGCTCCGGCACCGGGCGGCAGCGACTGATCTCGGC
GCGTACGGAAAGATCGAGGT

Downstream 100 bases:

>100_bases
TCTGGCACGGCCGTACGACTGTGCGTCCGCCGAAATGAGCCGAAAACCCGCTTCTAGCTGGGGATTTGACTCCGAGTTTC
CACTGACGTAACTTATTCCA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MSSILDELDWRGLIAQSTDRDALAAELAAGPMTLYSGFDPTAPSLHAGHLVPLLTLRRFQQAGHRPIVLAGGATGMIGDP
RDTGERTLQTADTVADWADRIRGQLERFVEFDESPTGAIVENNLSWTGALSTIEFLRDVGKYFSVNVMLDRDTVRRRLEG
EGISYTEFSYMLLQANDYVQLRKRHGCALQIGGSDQWGNIVAGVRLVRQKLGDTVHAMTTPLVTDSEGKKFGKSTGGGNL
WLDPEMTTPYAWYQYFINTADADVVNYLRWFTFLEAGELAELEEATRDRPHQRTAQRRLARELTTLVHGEDATRSVEHAS
QALFGRGELAALDEPTLAAALREASVAELTPSGPDLITDLLVATGLSASKGAARRTIAEGGVSVNNMKIDSDEWTPQASD
FLHGRWLVLRRGKRNIAGVQRVG

Sequences:

>Translated_423_residues
MSSILDELDWRGLIAQSTDRDALAAELAAGPMTLYSGFDPTAPSLHAGHLVPLLTLRRFQQAGHRPIVLAGGATGMIGDP
RDTGERTLQTADTVADWADRIRGQLERFVEFDESPTGAIVENNLSWTGALSTIEFLRDVGKYFSVNVMLDRDTVRRRLEG
EGISYTEFSYMLLQANDYVQLRKRHGCALQIGGSDQWGNIVAGVRLVRQKLGDTVHAMTTPLVTDSEGKKFGKSTGGGNL
WLDPEMTTPYAWYQYFINTADADVVNYLRWFTFLEAGELAELEEATRDRPHQRTAQRRLARELTTLVHGEDATRSVEHAS
QALFGRGELAALDEPTLAAALREASVAELTPSGPDLITDLLVATGLSASKGAARRTIAEGGVSVNNMKIDSDEWTPQASD
FLHGRWLVLRRGKRNIAGVQRVG
>Mature_422_residues
SSILDELDWRGLIAQSTDRDALAAELAAGPMTLYSGFDPTAPSLHAGHLVPLLTLRRFQQAGHRPIVLAGGATGMIGDPR
DTGERTLQTADTVADWADRIRGQLERFVEFDESPTGAIVENNLSWTGALSTIEFLRDVGKYFSVNVMLDRDTVRRRLEGE
GISYTEFSYMLLQANDYVQLRKRHGCALQIGGSDQWGNIVAGVRLVRQKLGDTVHAMTTPLVTDSEGKKFGKSTGGGNLW
LDPEMTTPYAWYQYFINTADADVVNYLRWFTFLEAGELAELEEATRDRPHQRTAQRRLARELTTLVHGEDATRSVEHASQ
ALFGRGELAALDEPTLAAALREASVAELTPSGPDLITDLLVATGLSASKGAARRTIAEGGVSVNNMKIDSDEWTPQASDF
LHGRWLVLRRGKRNIAGVQRVG

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=401, Percent_Identity=35.6608478802993, Blast_Score=215, Evalue=7e-56,
Organism=Escherichia coli, GI1787925, Length=419, Percent_Identity=47.4940334128878, Blast_Score=397, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25145802, Length=424, Percent_Identity=33.7264150943396, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI25145805, Length=308, Percent_Identity=32.4675324675325, Blast_Score=151, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6325160, Length=435, Percent_Identity=35.4022988505747, Blast_Score=250, Evalue=4e-67,
Organism=Drosophila melanogaster, GI20130383, Length=408, Percent_Identity=37.2549019607843, Blast_Score=246, Evalue=2e-65,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46453; Mature: 46322

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSILDELDWRGLIAQSTDRDALAAELAAGPMTLYSGFDPTAPSLHAGHLVPLLTLRRFQ
CCHHHHHHCCCCEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHH
QAGHRPIVLAGGATGMIGDPRDTGERTLQTADTVADWADRIRGQLERFVEFDESPTGAIV
HCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
ENNLSWTGALSTIEFLRDVGKYFSVNVMLDRDTVRRRLEGEGISYTEFSYMLLQANDYVQ
ECCCCHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHCCCCCCEEHEEEEEEECCCHHH
LRKRHGCALQIGGSDQWGNIVAGVRLVRQKLGDTVHAMTTPLVTDSEGKKFGKSTGGGNL
HHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCEECCCCHHHCCCCCCCCE
WLDPEMTTPYAWYQYFINTADADVVNYLRWFTFLEAGELAELEEATRDRPHQRTAQRRLA
EECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHH
RELTTLVHGEDATRSVEHASQALFGRGELAALDEPTLAAALREASVAELTPSGPDLITDL
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
LVATGLSASKGAARRTIAEGGVSVNNMKIDSDEWTPQASDFLHGRWLVLRRGKRNIAGVQ
HHHCCCCCCCCHHHHHHHHCCCEECCEEECCCCCCCCHHHHHCCEEEEEECCCCCCCCHH
RVG
CCC
>Mature Secondary Structure 
SSILDELDWRGLIAQSTDRDALAAELAAGPMTLYSGFDPTAPSLHAGHLVPLLTLRRFQ
CHHHHHHCCCCEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHH
QAGHRPIVLAGGATGMIGDPRDTGERTLQTADTVADWADRIRGQLERFVEFDESPTGAIV
HCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
ENNLSWTGALSTIEFLRDVGKYFSVNVMLDRDTVRRRLEGEGISYTEFSYMLLQANDYVQ
ECCCCHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHCCCCCCEEHEEEEEEECCCHHH
LRKRHGCALQIGGSDQWGNIVAGVRLVRQKLGDTVHAMTTPLVTDSEGKKFGKSTGGGNL
HHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCEECCCCHHHCCCCCCCCE
WLDPEMTTPYAWYQYFINTADADVVNYLRWFTFLEAGELAELEEATRDRPHQRTAQRRLA
EECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHH
RELTTLVHGEDATRSVEHASQALFGRGELAALDEPTLAAALREASVAELTPSGPDLITDL
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
LVATGLSASKGAARRTIAEGGVSVNNMKIDSDEWTPQASDFLHGRWLVLRRGKRNIAGVQ
HHHCCCCCCCCHHHHHHHHCCCEECCEEECCCCCCCCHHHHHCCEEEEEECCCCCCCCHH
RVG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA