Click here to switch to the map view.

The map label for this gene is gyrB [H]

Identifier: 108796987

GI number: 108796987

Start: 6173

End: 8200

Strand: Direct

Name: gyrB [H]

Synonym: Mmcs_0006

Alternate gene names: 108796987

Gene position: 6173-8200 (Clockwise)

Preceding gene: 108796986

Following gene: 108796988

Centisome position: 0.11

GC content: 64.0

Gene sequence:

>2028_bases
GTGGCTGCCCAGAAGAAGAATGCTCCGGCCGAGTACGGCGCGGATTCGATCAAGGTCCTCGAGGGGCTCGAAGCGGTCCG
CAAGCGACCGGGCATGTACATCGGCTCAACGGGCGAACGCGGTCTCCACCACCTGGTCTGGGAAGTCGTCGACAACGCCG
TCGACGAGGCGATGGCCGGTTTCGCCACCAAGGTCGAGGTGCGGATCCTCGAGAACGGCGGCGTCCAGGTCACCGACGAC
GGTCGTGGCATCCCGGTCGCCATGCACTCCACCGGCATCCCCACCGTCGACGTCGTGATGACCGTCCTGCACGCCGGCGG
CAAGTTCGAAGAGGGCGCCTACCAGGTGTCCGGCGGTCTGCACGGTGTGGGTGTGTCGGTGGTCAACGCGCTGTCCACCC
GGCTCGAGGCCGACATCCGCACCGACGGGTACGAGTGGTTTCAGACCTACGACCGTTCGGTGCCGGGCACCCTCAAGCAG
GGTGAGAAGACGAAGAAGACCGGCACGACCATCCGCTTCTGGGCCGATCCGGACATCTTCGAGACCACCAGCTACGACTT
CGAGACGATCGCCCGGCGTCTGCAGGAGATGGCGTTCCTCAACAAGGGCCTCACCATCGAATTGACCGACGAGCGGGTGA
CCGCCGAAGAGGTGGTCGACGACGTCGTCAGCGATCATGCCGAAGCGCCCAAGAGCGCCGAGGAGAAGTCGGCCGAGGCG
GCAAAACCGCAGAAGGTCAAGCACCGGGTCTTCCACTACCCGGGCGGTCTGGTCGACTTCGTCAAGCACATCAACCGCAC
GAAGACCCCGATCCAGCCCAGCGTCATCGACTTCGACGGTAAGGGCGAGGGCCACGAGGTCGAGATCGCGATGCAGTGGA
ACGCCGGCTACTCCGAGTCGGTGCACACGTTCGCCAACACGATCAACACCCACGAGGGCGGCACCCACGAAGAGGGGTTC
CGCACGGCGCTGACGTCGGTGGTGAACAAGTACGCCAAGGACAAGAAGCTCCTCAAGGACAAGGATCCGAACCTCACGGG
CGACGACATCCGGGAGGGCCTCGCCGCGGTCATCTCGGTGAAGGTGTCCCAGCCGCAGTTCGAAGGCCAGACGAAGACCA
AACTCGGCAACACCGAGGTCAAGTCGTTCGTGCAGAAGATCTGCAACGAACAGCTCAGCCACTGGTTCGAGGCCAATCCG
GCCGAGGCGAAAACCGTTGTGAACAAGGCGGTCTCGTCGGCGCAGGCGCGGCTGGCCGCCCGCAAGGCCCGGGAGTTGGT
GCGGCGCAAGAGCGCAACTGACATCGGCGGTCTGCCCGGCAAGCTCGCCGACTGCCGTTCGACCGATCCGAGCAAGTCGG
AACTGTATGTGGTGGAGGGTGACTCGGCCGGCGGTTCGGCCAAGAGCGGTCGCGACTCGATGTTCCAGGCGATCCTGCCG
TTACGGGGCAAGATCATCAACGTGGAGAAGGCGCGCATCGACCGCGTGCTGAAGAACACCGAAGTCCAGGCGATCATCAC
CGCGCTGGGCACGGGTATCCACGACGAGTTCGACATCAGCAAGCTGCGGTATCACAAGATCGTGCTGATGGCCGACGCAG
ACGTCGACGGCCAACACATCTCGACGCTGCTGCTGACACTGCTGTTCCGGTTCATGAAACCGTTGATCGAGAACGGTCAC
GTGTTCCTGGCGCAGCCGCCGCTGTACAAACTCAAATGGCAGCGCAGCACACCGGAATTCGCCTATTCGGACCGGGAGCG
CGACGGGTTGCTCGAAGCCGGCCGTGCCGCGGGCAAGCGGATCAACGCCGACGACGGCATCCAGCGCTACAAGGGTCTGG
GCGAGATGGACGCCAAGGAACTGTGGGAGACCACAATGGACCCGTCGGTGCGGGTGCTGCGCCAGATCACGCTCGACGAT
GCGGCCGCTGCCGACGAACTGTTCTCGATCCTGATGGGCGAGGACGTCGAGGCGCGCCGCAGCTTCATCACCCGAAACGC
CAAAGACGTTCGCTTCCTTGACGTTTAG

Upstream 100 bases:

>100_bases
CGCAGCGTCGGTCCTGACGGTATCTGCCGGTAGACTGGACAGGAACTACGCGGCGGTCCGTCGACCGTTCGCCCGCGAAC
CCCGAGGAGACGCGTCCAAC

Downstream 100 bases:

>100_bases
ACTACGTTCGACCGAAATACTTAGGAATCATCGATGACTGATACCACGTTGCCTCCTGGCGACGAAGCCGGAGACCGCAT
CGAACCGGTCGACATCCAGC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 675; Mature: 674

Protein sequence:

>675_residues
MAAQKKNAPAEYGADSIKVLEGLEAVRKRPGMYIGSTGERGLHHLVWEVVDNAVDEAMAGFATKVEVRILENGGVQVTDD
GRGIPVAMHSTGIPTVDVVMTVLHAGGKFEEGAYQVSGGLHGVGVSVVNALSTRLEADIRTDGYEWFQTYDRSVPGTLKQ
GEKTKKTGTTIRFWADPDIFETTSYDFETIARRLQEMAFLNKGLTIELTDERVTAEEVVDDVVSDHAEAPKSAEEKSAEA
AKPQKVKHRVFHYPGGLVDFVKHINRTKTPIQPSVIDFDGKGEGHEVEIAMQWNAGYSESVHTFANTINTHEGGTHEEGF
RTALTSVVNKYAKDKKLLKDKDPNLTGDDIREGLAAVISVKVSQPQFEGQTKTKLGNTEVKSFVQKICNEQLSHWFEANP
AEAKTVVNKAVSSAQARLAARKARELVRRKSATDIGGLPGKLADCRSTDPSKSELYVVEGDSAGGSAKSGRDSMFQAILP
LRGKIINVEKARIDRVLKNTEVQAIITALGTGIHDEFDISKLRYHKIVLMADADVDGQHISTLLLTLLFRFMKPLIENGH
VFLAQPPLYKLKWQRSTPEFAYSDRERDGLLEAGRAAGKRINADDGIQRYKGLGEMDAKELWETTMDPSVRVLRQITLDD
AAAADELFSILMGEDVEARRSFITRNAKDVRFLDV

Sequences:

>Translated_675_residues
MAAQKKNAPAEYGADSIKVLEGLEAVRKRPGMYIGSTGERGLHHLVWEVVDNAVDEAMAGFATKVEVRILENGGVQVTDD
GRGIPVAMHSTGIPTVDVVMTVLHAGGKFEEGAYQVSGGLHGVGVSVVNALSTRLEADIRTDGYEWFQTYDRSVPGTLKQ
GEKTKKTGTTIRFWADPDIFETTSYDFETIARRLQEMAFLNKGLTIELTDERVTAEEVVDDVVSDHAEAPKSAEEKSAEA
AKPQKVKHRVFHYPGGLVDFVKHINRTKTPIQPSVIDFDGKGEGHEVEIAMQWNAGYSESVHTFANTINTHEGGTHEEGF
RTALTSVVNKYAKDKKLLKDKDPNLTGDDIREGLAAVISVKVSQPQFEGQTKTKLGNTEVKSFVQKICNEQLSHWFEANP
AEAKTVVNKAVSSAQARLAARKARELVRRKSATDIGGLPGKLADCRSTDPSKSELYVVEGDSAGGSAKSGRDSMFQAILP
LRGKIINVEKARIDRVLKNTEVQAIITALGTGIHDEFDISKLRYHKIVLMADADVDGQHISTLLLTLLFRFMKPLIENGH
VFLAQPPLYKLKWQRSTPEFAYSDRERDGLLEAGRAAGKRINADDGIQRYKGLGEMDAKELWETTMDPSVRVLRQITLDD
AAAADELFSILMGEDVEARRSFITRNAKDVRFLDV
>Mature_674_residues
AAQKKNAPAEYGADSIKVLEGLEAVRKRPGMYIGSTGERGLHHLVWEVVDNAVDEAMAGFATKVEVRILENGGVQVTDDG
RGIPVAMHSTGIPTVDVVMTVLHAGGKFEEGAYQVSGGLHGVGVSVVNALSTRLEADIRTDGYEWFQTYDRSVPGTLKQG
EKTKKTGTTIRFWADPDIFETTSYDFETIARRLQEMAFLNKGLTIELTDERVTAEEVVDDVVSDHAEAPKSAEEKSAEAA
KPQKVKHRVFHYPGGLVDFVKHINRTKTPIQPSVIDFDGKGEGHEVEIAMQWNAGYSESVHTFANTINTHEGGTHEEGFR
TALTSVVNKYAKDKKLLKDKDPNLTGDDIREGLAAVISVKVSQPQFEGQTKTKLGNTEVKSFVQKICNEQLSHWFEANPA
EAKTVVNKAVSSAQARLAARKARELVRRKSATDIGGLPGKLADCRSTDPSKSELYVVEGDSAGGSAKSGRDSMFQAILPL
RGKIINVEKARIDRVLKNTEVQAIITALGTGIHDEFDISKLRYHKIVLMADADVDGQHISTLLLTLLFRFMKPLIENGHV
FLAQPPLYKLKWQRSTPEFAYSDRERDGLLEAGRAAGKRINADDGIQRYKGLGEMDAKELWETTMDPSVRVLRQITLDDA
AAADELFSILMGEDVEARRSFITRNAKDVRFLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=708, Percent_Identity=24.1525423728814, Blast_Score=107, Evalue=5e-23,
Organism=Homo sapiens, GI19913406, Length=663, Percent_Identity=23.6802413273001, Blast_Score=104, Evalue=3e-22,
Organism=Escherichia coli, GI48994957, Length=566, Percent_Identity=51.4134275618375, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI1789408, Length=666, Percent_Identity=37.987987987988, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17535065, Length=700, Percent_Identity=23.1428571428571, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI212645845, Length=593, Percent_Identity=22.4283305227656, Blast_Score=105, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI212645657, Length=259, Percent_Identity=27.027027027027, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=324, Percent_Identity=27.1604938271605, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17136538, Length=668, Percent_Identity=24.5508982035928, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 74348; Mature: 74217

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAQKKNAPAEYGADSIKVLEGLEAVRKRPGMYIGSTGERGLHHLVWEVVDNAVDEAMAG
CCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHC
FATKVEVRILENGGVQVTDDGRGIPVAMHSTGIPTVDVVMTVLHAGGKFEEGAYQVSGGL
CCEEEEEEEEECCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGVSVVNALSTRLEADIRTDGYEWFQTYDRSVPGTLKQGEKTKKTGTTIRFWADPDIF
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHCCCCCCCCCCEEEEECCCCCC
ETTSYDFETIARRLQEMAFLNKGLTIELTDERVTAEEVVDDVVSDHAEAPKSAEEKSAEA
CCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
AKPQKVKHRVFHYPGGLVDFVKHINRTKTPIQPSVIDFDGKGEGHEVEIAMQWNAGYSES
CCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCEEEEEEEECCCCCHH
VHTFANTINTHEGGTHEEGFRTALTSVVNKYAKDKKLLKDKDPNLTGDDIREGLAAVISV
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHE
KVSQPQFEGQTKTKLGNTEVKSFVQKICNEQLSHWFEANPAEAKTVVNKAVSSAQARLAA
EECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RKARELVRRKSATDIGGLPGKLADCRSTDPSKSELYVVEGDSAGGSAKSGRDSMFQAILP
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
LRGKIINVEKARIDRVLKNTEVQAIITALGTGIHDEFDISKLRYHKIVLMADADVDGQHI
CCCEEEEHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHEEEEEEEEEECCCCCHHHH
STLLLTLLFRFMKPLIENGHVFLAQPPLYKLKWQRSTPEFAYSDRERDGLLEAGRAAGKR
HHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCC
INADDGIQRYKGLGEMDAKELWETTMDPSVRVLRQITLDDAAAADELFSILMGEDVEARR
CCCCHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SFITRNAKDVRFLDV
HHHHCCCCCEEEECC
>Mature Secondary Structure 
AAQKKNAPAEYGADSIKVLEGLEAVRKRPGMYIGSTGERGLHHLVWEVVDNAVDEAMAG
CCCCCCCCHHHCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHC
FATKVEVRILENGGVQVTDDGRGIPVAMHSTGIPTVDVVMTVLHAGGKFEEGAYQVSGGL
CCEEEEEEEEECCCEEEECCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEECCCC
HGVGVSVVNALSTRLEADIRTDGYEWFQTYDRSVPGTLKQGEKTKKTGTTIRFWADPDIF
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHCCCCCCCCCCEEEEECCCCCC
ETTSYDFETIARRLQEMAFLNKGLTIELTDERVTAEEVVDDVVSDHAEAPKSAEEKSAEA
CCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
AKPQKVKHRVFHYPGGLVDFVKHINRTKTPIQPSVIDFDGKGEGHEVEIAMQWNAGYSES
CCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCEEEEEEEECCCCCHH
VHTFANTINTHEGGTHEEGFRTALTSVVNKYAKDKKLLKDKDPNLTGDDIREGLAAVISV
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHE
KVSQPQFEGQTKTKLGNTEVKSFVQKICNEQLSHWFEANPAEAKTVVNKAVSSAQARLAA
EECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RKARELVRRKSATDIGGLPGKLADCRSTDPSKSELYVVEGDSAGGSAKSGRDSMFQAILP
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
LRGKIINVEKARIDRVLKNTEVQAIITALGTGIHDEFDISKLRYHKIVLMADADVDGQHI
CCCEEEEHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHEEEEEEEEEECCCCCHHHH
STLLLTLLFRFMKPLIENGHVFLAQPPLYKLKWQRSTPEFAYSDRERDGLLEAGRAAGKR
HHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCC
INADDGIQRYKGLGEMDAKELWETTMDPSVRVLRQITLDDAAAADELFSILMGEDVEARR
CCCCHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SFITRNAKDVRFLDV
HHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8878580 [H]