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The map label for this gene is rlmI [H]

Identifier: 108764079

GI number: 108764079

Start: 6391979

End: 6392965

Strand: Reverse

Name: rlmI [H]

Synonym: MXAN_5117

Alternate gene names: 108764079

Gene position: 6392965-6391979 (Counterclockwise)

Preceding gene: 108758244

Following gene: 108759758

Centisome position: 69.95

GC content: 70.52

Gene sequence:

>987_bases
ATGGGCACACTGGCTTCTTCTCTCGTGGAGACGCTGCGCGCCTCGCGCGGCCGGCGCGGGCCGCTGCTGGGAGACGCGCG
GACCACGGCCTGGCGCGTGGTAAACGCCGAAGCGGACGGTGTGCCGGATGTGACGGCGGATGTGTTTGGTGACGTGTACG
TCATCAGCCTCTACCGGGACTTCACGCCGGACGAGGAGGAGACGCTGCTCGACGCGGCCGTGGCCGCCTGGGCGCCTCGA
AGCCTCTACCTCAAGCGCCGGCCCCGGGAGGCGCGTGTCCTGGCCAACGTGGCGAAGGAGTCGCTCGCACCCGAGGCGCC
CGCCCGGGGCGAAGCGGTGGAGTCCTTCACCGCGCTCGAAAACGGCCTGACGTTCCTCATCCGTCCGGCGCAGGGGCTGT
CGGTGGGGCTGTACCTGGACATGCGGGACACGCGGGCGTGGCTGCTGTCGCAGGCGCGGGGCCTCACCGTGCTCAACCTC
TTCTCGTACACCTGTGCCTTTGGCGTCGTGGCCATGGCGGGCGGCGCGAAGCGGGCGCTCAACCTGGATGCGAGCCGGCG
CGTGCTGGAGTGGGGCGAGGAGAACGCGCGCCTCAATGGCCAGACGGTGGACCGATATGACTACGTGGCCGGTGACGTGT
TCGATTGGCTGAAGCGCCTGGCGAAGAAGGGCGAGTCCTTCGACATCGTCATCTCGGACCCGCCGTCGTTCTCGACGACG
CGCAGTGGCCGGTTCTCCGCGGCGCGCGACTATGCCCGGCTGGCCGAGGCCGCCGCGCGCGTCGTCAGTCCGGGAGGCCA
ACTGGTGGCGTGCTGCAACCACGCGGGACTGGCTGCCCGCCGCTTCGAGGCCATGGTGCTCGAAGGGGTGTCCCAGGCGG
GACGCCAGGGGAAGTCGTTGGGCTCGCTGGCGCCGTCCGCGTTGGATTTCCCACCGCCGCCCGGACAGGAGCCCGCGCTG
AAGGTGCACCGGGTGCAGGTCCGTTAG

Upstream 100 bases:

>100_bases
GGCAGTTGCTGGGGCTCGACTACGTGGCGCTTTCCCGTGCGGCGACCGAGGCGCTGGAGCGTGTCTGGACGCGCGCGCGT
CTGGGCAGTTGAGCGGCGGC

Downstream 100 bases:

>100_bases
GAATAGAGGCCTGCCGAGCTTCGCCCAAGTTTGTTGCCACTCCGCGGCTCGGCACAGGTAGGCTCGCCGGGCCATGCCTC
CCGCCGCCCCGCAGCGCGAC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MGTLASSLVETLRASRGRRGPLLGDARTTAWRVVNAEADGVPDVTADVFGDVYVISLYRDFTPDEEETLLDAAVAAWAPR
SLYLKRRPREARVLANVAKESLAPEAPARGEAVESFTALENGLTFLIRPAQGLSVGLYLDMRDTRAWLLSQARGLTVLNL
FSYTCAFGVVAMAGGAKRALNLDASRRVLEWGEENARLNGQTVDRYDYVAGDVFDWLKRLAKKGESFDIVISDPPSFSTT
RSGRFSAARDYARLAEAAARVVSPGGQLVACCNHAGLAARRFEAMVLEGVSQAGRQGKSLGSLAPSALDFPPPPGQEPAL
KVHRVQVR

Sequences:

>Translated_328_residues
MGTLASSLVETLRASRGRRGPLLGDARTTAWRVVNAEADGVPDVTADVFGDVYVISLYRDFTPDEEETLLDAAVAAWAPR
SLYLKRRPREARVLANVAKESLAPEAPARGEAVESFTALENGLTFLIRPAQGLSVGLYLDMRDTRAWLLSQARGLTVLNL
FSYTCAFGVVAMAGGAKRALNLDASRRVLEWGEENARLNGQTVDRYDYVAGDVFDWLKRLAKKGESFDIVISDPPSFSTT
RSGRFSAARDYARLAEAAARVVSPGGQLVACCNHAGLAARRFEAMVLEGVSQAGRQGKSLGSLAPSALDFPPPPGQEPAL
KVHRVQVR
>Mature_327_residues
GTLASSLVETLRASRGRRGPLLGDARTTAWRVVNAEADGVPDVTADVFGDVYVISLYRDFTPDEEETLLDAAVAAWAPRS
LYLKRRPREARVLANVAKESLAPEAPARGEAVESFTALENGLTFLIRPAQGLSVGLYLDMRDTRAWLLSQARGLTVLNLF
SYTCAFGVVAMAGGAKRALNLDASRRVLEWGEENARLNGQTVDRYDYVAGDVFDWLKRLAKKGESFDIVISDPPSFSTTR
SGRFSAARDYARLAEAAARVVSPGGQLVACCNHAGLAARRFEAMVLEGVSQAGRQGKSLGSLAPSALDFPPPPGQEPALK
VHRVQVR

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=268, Percent_Identity=27.9850746268657, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787181, Length=230, Percent_Identity=30.4347826086957, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 35437; Mature: 35305

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTLASSLVETLRASRGRRGPLLGDARTTAWRVVNAEADGVPDVTADVFGDVYVISLYRD
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCEEEEEEECC
FTPDEEETLLDAAVAAWAPRSLYLKRRPREARVLANVAKESLAPEAPARGEAVESFTALE
CCCCHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
NGLTFLIRPAQGLSVGLYLDMRDTRAWLLSQARGLTVLNLFSYTCAFGVVAMAGGAKRAL
CCCEEEEECCCCCEEEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEE
NLDASRRVLEWGEENARLNGQTVDRYDYVAGDVFDWLKRLAKKGESFDIVISDPPSFSTT
CCCHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
RSGRFSAARDYARLAEAAARVVSPGGQLVACCNHAGLAARRFEAMVLEGVSQAGRQGKSL
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHH
GSLAPSALDFPPPPGQEPALKVHRVQVR
HHCCCCCCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
GTLASSLVETLRASRGRRGPLLGDARTTAWRVVNAEADGVPDVTADVFGDVYVISLYRD
CHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCEEEEEEECC
FTPDEEETLLDAAVAAWAPRSLYLKRRPREARVLANVAKESLAPEAPARGEAVESFTALE
CCCCHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
NGLTFLIRPAQGLSVGLYLDMRDTRAWLLSQARGLTVLNLFSYTCAFGVVAMAGGAKRAL
CCCEEEEECCCCCEEEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEE
NLDASRRVLEWGEENARLNGQTVDRYDYVAGDVFDWLKRLAKKGESFDIVISDPPSFSTT
CCCHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
RSGRFSAARDYARLAEAAARVVSPGGQLVACCNHAGLAARRFEAMVLEGVSQAGRQGKSL
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHH
GSLAPSALDFPPPPGQEPALKVHRVQVR
HHCCCCCCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA