Click here to switch to the map view.

The map label for this gene is alkA [H]

Identifier: 108763302

GI number: 108763302

Start: 6518965

End: 6520464

Strand: Reverse

Name: alkA [H]

Synonym: MXAN_5220

Alternate gene names: 108763302

Gene position: 6520464-6518965 (Counterclockwise)

Preceding gene: 108763118

Following gene: 108761935

Centisome position: 71.34

GC content: 67.8

Gene sequence:

>1500_bases
ATGCGCGGGGCAAAGAGCTGGCAAGGCCTCCAACTCTATACCGTTGGCCACTCGACGCGAACGCTCGACGAGCTCATCGC
GCTCCTTCGACCGTTCGGCGTCTCCACGGTGGTTGATATCAGGACGATCCCGCGCTCGCGCCACAATCCGCAATTCGAAA
GCGAGGCGCTTCGCTCCGCGCTTCGCCGGCATCACCTCCGTTACGTCCATCTCCCCGCGCTCGGAGGGCTGCGGCACGCG
CGGGGTGACTCACCGAACACGGGGTGGCGCAATGCGAGCTTCCGCGGGTACGCGGACTACATGCTGACGGACGCGTTCGA
GTCTGGGCTGGCCGAGCTGCGTGAGCTGGTCGCGGAAGGCACGGTAGCGTTGATGTGCGCCGAAGCCGTGCCCTGGCGTT
GCCATCGCTCCCTGGTCGCAGACGCCCTGACAGTTCGCGGCGCGCAGGTCGAGCACATCACCAGCCGAACCCGGTCGACG
CCGCACCGGATGACCGACTTCGCTCATGTCGATGGCACGCGGCTCACGTATCCGGCGGGCCTCGGCTCATCCCTCGATAC
GCGCGCGCCGTTTCACCTCGAGGCGACCGTGCGCGTACTGCAGCGGCGGCCGACGAACCTCGTCGACGTCTGGGAGGGCG
GACGGTACCTGCGAGCGCTCACGGTCTCCGATGGCTTCGTGCTGGTCGAGGTCTCGAATCAGGGGACGGTGGACGAGCCG
CAGGTCCGGTTCCGCGTGCTCGATGGGGATGACTCGCGCGGCGCGCATGCCGAGATCTCTCGGGTGTTGCGGCGGGGGCT
CGGACTCGATGTGGACCCGGAGCCGCTCGATCGGCTGCTCCAATCCGAGCGCAAGCTCGGCCCCATTGTGCGGGCGCTGC
GCGGTATGCGGCCGCCACGGTTCCCCAGCCTCTTCGAGACGTTCGCGAACGTCATCCCGTTCCAGCAGGTCAGCCTGGAC
GCCGGGGTCGCAGTCGTTCGCCGTTTGGTAGCGCGCTTCGGCCGGTTCTTGCCGCACGAGGGGCAAGTACGGTACGCCTT
TCCTACTGCTGCTGCGCTCGCCGAGGCGCGGCTCGACGCGATTCGCTCCTGTGGCTTGAGCGCGAGGAAGGCCGAGGCGC
TCCGCGCGGCCGCCGCCGCCATCCAAGCGGGCGATGTGACAGAGGCCATGCTCTCGCAAATGAGCAGCGCCGAGGCGATG
CGGATGCTCACTGGGCTTCACGGTATTGGCCCCTGGAGCGCGGCCTTGGTTCTCCTGCGCGGCTTGGGCCGGCTGGACGT
GTTCCCGGAGGGCGATGTCGGGGTCATTCGCGGGCTCTCGGGATTGATGCATGTCGAGCCGGGACCTGCTCTCGAGCGCC
TGATCCGCCGCTTCGGCGAGCAGCGTGGGTATTTGTATTTCTGCTCGCTCGGTAGCGCGCTCTTGGCCCGAGGCCTCATT
CATGCCGCCGACGCTGGGCCACGGCGAAGCCTTCTAATCGCGCCGGAGTCGCACCATTGA

Upstream 100 bases:

>100_bases
CCTCGAGAAAGGCCTGCGCCATGACGTGGAGGCGATCGAGGAGAGCGCGTTCCTGCTCACGCTCGGATGGAAGAGCCCAT
GAGAGAGGGGTAGAAAGTCG

Downstream 100 bases:

>100_bases
GGTCAACGCAGCGATGCCTGCCGATGTGCAACTCATGCAGAGGAGGCGGTTCGCTCTCGCTCTAGGGGAGCCGCGCGAAG
CCGAGTCGCCGCTCAGGAGC

Product: HhH-GPD domain-containing protein

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosidase [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MRGAKSWQGLQLYTVGHSTRTLDELIALLRPFGVSTVVDIRTIPRSRHNPQFESEALRSALRRHHLRYVHLPALGGLRHA
RGDSPNTGWRNASFRGYADYMLTDAFESGLAELRELVAEGTVALMCAEAVPWRCHRSLVADALTVRGAQVEHITSRTRST
PHRMTDFAHVDGTRLTYPAGLGSSLDTRAPFHLEATVRVLQRRPTNLVDVWEGGRYLRALTVSDGFVLVEVSNQGTVDEP
QVRFRVLDGDDSRGAHAEISRVLRRGLGLDVDPEPLDRLLQSERKLGPIVRALRGMRPPRFPSLFETFANVIPFQQVSLD
AGVAVVRRLVARFGRFLPHEGQVRYAFPTAAALAEARLDAIRSCGLSARKAEALRAAAAAIQAGDVTEAMLSQMSSAEAM
RMLTGLHGIGPWSAALVLLRGLGRLDVFPEGDVGVIRGLSGLMHVEPGPALERLIRRFGEQRGYLYFCSLGSALLARGLI
HAADAGPRRSLLIAPESHH

Sequences:

>Translated_499_residues
MRGAKSWQGLQLYTVGHSTRTLDELIALLRPFGVSTVVDIRTIPRSRHNPQFESEALRSALRRHHLRYVHLPALGGLRHA
RGDSPNTGWRNASFRGYADYMLTDAFESGLAELRELVAEGTVALMCAEAVPWRCHRSLVADALTVRGAQVEHITSRTRST
PHRMTDFAHVDGTRLTYPAGLGSSLDTRAPFHLEATVRVLQRRPTNLVDVWEGGRYLRALTVSDGFVLVEVSNQGTVDEP
QVRFRVLDGDDSRGAHAEISRVLRRGLGLDVDPEPLDRLLQSERKLGPIVRALRGMRPPRFPSLFETFANVIPFQQVSLD
AGVAVVRRLVARFGRFLPHEGQVRYAFPTAAALAEARLDAIRSCGLSARKAEALRAAAAAIQAGDVTEAMLSQMSSAEAM
RMLTGLHGIGPWSAALVLLRGLGRLDVFPEGDVGVIRGLSGLMHVEPGPALERLIRRFGEQRGYLYFCSLGSALLARGLI
HAADAGPRRSLLIAPESHH
>Mature_499_residues
MRGAKSWQGLQLYTVGHSTRTLDELIALLRPFGVSTVVDIRTIPRSRHNPQFESEALRSALRRHHLRYVHLPALGGLRHA
RGDSPNTGWRNASFRGYADYMLTDAFESGLAELRELVAEGTVALMCAEAVPWRCHRSLVADALTVRGAQVEHITSRTRST
PHRMTDFAHVDGTRLTYPAGLGSSLDTRAPFHLEATVRVLQRRPTNLVDVWEGGRYLRALTVSDGFVLVEVSNQGTVDEP
QVRFRVLDGDDSRGAHAEISRVLRRGLGLDVDPEPLDRLLQSERKLGPIVRALRGMRPPRFPSLFETFANVIPFQQVSLD
AGVAVVRRLVARFGRFLPHEGQVRYAFPTAAALAEARLDAIRSCGLSARKAEALRAAAAAIQAGDVTEAMLSQMSSAEAM
RMLTGLHGIGPWSAALVLLRGLGRLDVFPEGDVGVIRGLSGLMHVEPGPALERLIRRFGEQRGYLYFCSLGSALLARGLI
HAADAGPRRSLLIAPESHH

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG5483

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320990, Length=192, Percent_Identity=29.6875, Blast_Score=77, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010316
- InterPro:   IPR000035
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR012904 [H]

Pfam domain/function: PF00730 HhH-GPD; PF07934 OGG_N [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 54793; Mature: 54793

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGAKSWQGLQLYTVGHSTRTLDELIALLRPFGVSTVVDIRTIPRSRHNPQFESEALRSA
CCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCHHEEEHHHCCCCCCCCCHHHHHHHHH
LRRHHLRYVHLPALGGLRHARGDSPNTGWRNASFRGYADYMLTDAFESGLAELRELVAEG
HHHCCCEEEEECCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
TVALMCAEAVPWRCHRSLVADALTVRGAQVEHITSRTRSTPHRMTDFAHVDGTRLTYPAG
HHEEHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHCCCCCEEEECCC
LGSSLDTRAPFHLEATVRVLQRRPTNLVDVWEGGRYLRALTVSDGFVLVEVSNQGTVDEP
CCCCCCCCCCEEHHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCEEEEEECCCCCCCCC
QVRFRVLDGDDSRGAHAEISRVLRRGLGLDVDPEPLDRLLQSERKLGPIVRALRGMRPPR
CEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FPSLFETFANVIPFQQVSLDAGVAVVRRLVARFGRFLPHEGQVRYAFPTAAALAEARLDA
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHH
IRSCGLSARKAEALRAAAAAIQAGDVTEAMLSQMSSAEAMRMLTGLHGIGPWSAALVLLR
HHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
GLGRLDVFPEGDVGVIRGLSGLMHVEPGPALERLIRRFGEQRGYLYFCSLGSALLARGLI
CCCCCCCCCCCCHHHHHHCCCCEECCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHH
HAADAGPRRSLLIAPESHH
HHCCCCCCCEEEECCCCCC
>Mature Secondary Structure
MRGAKSWQGLQLYTVGHSTRTLDELIALLRPFGVSTVVDIRTIPRSRHNPQFESEALRSA
CCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCHHEEEHHHCCCCCCCCCHHHHHHHHH
LRRHHLRYVHLPALGGLRHARGDSPNTGWRNASFRGYADYMLTDAFESGLAELRELVAEG
HHHCCCEEEEECCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
TVALMCAEAVPWRCHRSLVADALTVRGAQVEHITSRTRSTPHRMTDFAHVDGTRLTYPAG
HHEEHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHCCCCCEEEECCC
LGSSLDTRAPFHLEATVRVLQRRPTNLVDVWEGGRYLRALTVSDGFVLVEVSNQGTVDEP
CCCCCCCCCCEEHHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCEEEEEECCCCCCCCC
QVRFRVLDGDDSRGAHAEISRVLRRGLGLDVDPEPLDRLLQSERKLGPIVRALRGMRPPR
CEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FPSLFETFANVIPFQQVSLDAGVAVVRRLVARFGRFLPHEGQVRYAFPTAAALAEARLDA
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHH
IRSCGLSARKAEALRAAAAAIQAGDVTEAMLSQMSSAEAMRMLTGLHGIGPWSAALVLLR
HHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
GLGRLDVFPEGDVGVIRGLSGLMHVEPGPALERLIRRFGEQRGYLYFCSLGSALLARGLI
CCCCCCCCCCCCHHHHHHCCCCEECCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHH
HAADAGPRRSLLIAPESHH
HHCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8376346; 9384377 [H]