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The map label for this gene is trmJ [H]

Identifier: 108763165

GI number: 108763165

Start: 2813772

End: 2814494

Strand: Reverse

Name: trmJ [H]

Synonym: MXAN_2416

Alternate gene names: 108763165

Gene position: 2814494-2813772 (Counterclockwise)

Preceding gene: 108757499

Following gene: 108760801

Centisome position: 30.79

GC content: 73.44

Gene sequence:

>723_bases
ATGGTGCTGCCTGTCCGATTCGTCCTCATGCGCCCCCGCAACGCGGAAAACCTGGGCGCCGCCGCCCGGGCCTTGAAGAA
CTGCGGCCTGTCCGACTGGGTCTGGGTCCGCCCGGAGGTGGAGGATTTGGAGCCGGCGCGAAGGCTCGCCGTCCACGCCG
GGGACGTGCTGGACGCGGCGCGGCGCGCGGACACGCTGGAAGAGGCCGTGGCGGACTGTGTCTGGGTGGTGGGGACCAGC
TCGCGCAAGGTCGAAGGCAAGCGCCGGCTTCCCCCTCGCGCGGTGGGTGAGGAGCTGGTGGCCCGGGCGCCCCAAGGGCC
CGTGGCGCTCGTCTTCGGTGACGAGCGCAGTGGACTCACCAACGCGGAGGTGGAGCGCGTGCACGACCTGTCCGCCGTGC
CGACCGCGCCCGAACAGCCCTCCATCAACCTGGCGCAGGCCGTGCTGCTGTACGCCTACGAGGTCCGCGTGGCGATGCTG
GAGGCCGCCGCCACGCCGCCCGGCCCGCTGCCCGCGGCGGCCACGGACGCGGAGCTCGCGCAGGTGGAGTCCACGCTGGA
ATCCGTCCTCACGACGGGAGGCTTCCTCGTGGACGCCCAGCCGGGCCGCACGGCGGTGCGGGACTTGTTCGCACCGCTGC
GGCGCTCACGGCTGACGCGGAAGGAAGCCCGCTTGTGGCTGGCCGCGCTGCACAGCCTCCGCAAGCGTCAGCCCGCGCCC
TAG

Upstream 100 bases:

>100_bases
GCGAAGGCTTTCGAGGCCCCCAGGCTAGCGGAGTCCGCGACGCGGCGGGACCTCCATCCGCGGTCCTCAGGCTGCGAGAA
ACCGCTAAGGTGCCCCGCGC

Downstream 100 bases:

>100_bases
AGGGAGCGCGGCTTCTGCGGCAGCCCCGTCCCCTTCCCCACGCCAATCCCCCCGGAGCCGGACGTCGGCGGCCCCTTGCG
CCCCGCCATGCGCACCGCGC

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MVLPVRFVLMRPRNAENLGAAARALKNCGLSDWVWVRPEVEDLEPARRLAVHAGDVLDAARRADTLEEAVADCVWVVGTS
SRKVEGKRRLPPRAVGEELVARAPQGPVALVFGDERSGLTNAEVERVHDLSAVPTAPEQPSINLAQAVLLYAYEVRVAML
EAAATPPGPLPAAATDAELAQVESTLESVLTTGGFLVDAQPGRTAVRDLFAPLRRSRLTRKEARLWLAALHSLRKRQPAP

Sequences:

>Translated_240_residues
MVLPVRFVLMRPRNAENLGAAARALKNCGLSDWVWVRPEVEDLEPARRLAVHAGDVLDAARRADTLEEAVADCVWVVGTS
SRKVEGKRRLPPRAVGEELVARAPQGPVALVFGDERSGLTNAEVERVHDLSAVPTAPEQPSINLAQAVLLYAYEVRVAML
EAAATPPGPLPAAATDAELAQVESTLESVLTTGGFLVDAQPGRTAVRDLFAPLRRSRLTRKEARLWLAALHSLRKRQPAP
>Mature_240_residues
MVLPVRFVLMRPRNAENLGAAARALKNCGLSDWVWVRPEVEDLEPARRLAVHAGDVLDAARRADTLEEAVADCVWVVGTS
SRKVEGKRRLPPRAVGEELVARAPQGPVALVFGDERSGLTNAEVERVHDLSAVPTAPEQPSINLAQAVLLYAYEVRVAML
EAAATPPGPLPAAATDAELAQVESTLESVLTTGGFLVDAQPGRTAVRDLFAPLRRSRLTRKEARLWLAALHSLRKRQPAP

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=250, Percent_Identity=37.6, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1790865, Length=157, Percent_Identity=38.2165605095541, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 25976; Mature: 25976

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLPVRFVLMRPRNAENLGAAARALKNCGLSDWVWVRPEVEDLEPARRLAVHAGDVLDAA
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHCCHHHHHHHHHHHHHHHH
RRADTLEEAVADCVWVVGTSSRKVEGKRRLPPRAVGEELVARAPQGPVALVFGDERSGLT
HHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCC
NAEVERVHDLSAVPTAPEQPSINLAQAVLLYAYEVRVAMLEAAATPPGPLPAAATDAELA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
QVESTLESVLTTGGFLVDAQPGRTAVRDLFAPLRRSRLTRKEARLWLAALHSLRKRQPAP
HHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVLPVRFVLMRPRNAENLGAAARALKNCGLSDWVWVRPEVEDLEPARRLAVHAGDVLDAA
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCHHHCCHHHHHHHHHHHHHHHH
RRADTLEEAVADCVWVVGTSSRKVEGKRRLPPRAVGEELVARAPQGPVALVFGDERSGLT
HHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCC
NAEVERVHDLSAVPTAPEQPSINLAQAVLLYAYEVRVAMLEAAATPPGPLPAAATDAELA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
QVESTLESVLTTGGFLVDAQPGRTAVRDLFAPLRRSRLTRKEARLWLAALHSLRKRQPAP
HHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA