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The map label for this gene is rluB [H]

Identifier: 108760801

GI number: 108760801

Start: 2812526

End: 2813332

Strand: Reverse

Name: rluB [H]

Synonym: MXAN_2415

Alternate gene names: 108760801

Gene position: 2813332-2812526 (Counterclockwise)

Preceding gene: 108763165

Following gene: 108759074

Centisome position: 30.78

GC content: 71.87

Gene sequence:

>807_bases
ATGTCTCGCAAGCCCGACAAGCCGAAGCCGCCGCCGCAACACAAACGCTGGGAGGGCAAGGAGAAGCCGGACTGGCTGTC
GCGCGCGCTGGCCCGCGCGGGCGTCTTTCCCCAGAAGGAAGCCGAGGACGCCATCCGCGCCGGCCGCGTCAGCATCAACG
GCCGCGTGGCGAAGATGGCGCTGACGCCCGTGCCGCCGGGCGCCACCGTGAAGGTGGACGGACTCGTCGTGTCCCTGGAG
GCCCCGCCCACGCGGGTGCTGGCCTTCCACAAGCCCGCGGGCCTGCTGTCCTCCACGGAGCGGCAGCACCGCACCGGCAC
CGTCTTCGAGGCGCTGCTGCCCCAGCTCCCGCCGGAGCTGGCTGGATACACGTGGCACGCCGTGGGCCGCCTGGACGTGG
ATTCCACGGGCCTGCTGCTCTTCACCAACGACGACAAGCTGGTGGCCCACGCCACGTCCCCGGACGCGCACCTGCCCAAG
CGGTACGTGGCCACCGTCTTCAGCACCGCGAGCGACGAGCGGGTGGAGCCTCTGCGCCGGGGCATGACGTTGGATGATGG
CCCCGCCCGCCCCGCGAAGGTGAACGTGCGGGACGAGCACACGGTGGAGGTGACGCTCACGGAAGGCCGGCACCACCAGG
TGAAGCGGATGCTCGGCGCGGTGGGCCTTCCCGTGCGCGCGCTGCACCGCGAGGCCGTGGGCAGCGTGGACCTGGAAGGC
ATCCCCGAAGGCACCTTCCGGCTGCTGACGGACGACGAGGTCCGCGAGGGGCTTCGCTACGAAGGACGCACGCCCTTCGG
CCCCTAG

Upstream 100 bases:

>100_bases
CGGGCGCCCCCCGGCCAGATGGCCTCATCGCGACAGCGCCGCCATGGCCCATACAACTCCCGCCCCCGCCCGAGGCCCGG
CCGGGTACAGTGCCGCGCCC

Downstream 100 bases:

>100_bases
GGCCTTCGTTCCCTCCCCACGAAGCATGGCTGGGTGCCGCGCGACATGCGGGCCCACGCCCCCGCGTGCGAGGAGCGCCA
TGGGCACCCCCTGGTGGCGC

Product: RsuA family pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MSRKPDKPKPPPQHKRWEGKEKPDWLSRALARAGVFPQKEAEDAIRAGRVSINGRVAKMALTPVPPGATVKVDGLVVSLE
APPTRVLAFHKPAGLLSSTERQHRTGTVFEALLPQLPPELAGYTWHAVGRLDVDSTGLLLFTNDDKLVAHATSPDAHLPK
RYVATVFSTASDERVEPLRRGMTLDDGPARPAKVNVRDEHTVEVTLTEGRHHQVKRMLGAVGLPVRALHREAVGSVDLEG
IPEGTFRLLTDDEVREGLRYEGRTPFGP

Sequences:

>Translated_268_residues
MSRKPDKPKPPPQHKRWEGKEKPDWLSRALARAGVFPQKEAEDAIRAGRVSINGRVAKMALTPVPPGATVKVDGLVVSLE
APPTRVLAFHKPAGLLSSTERQHRTGTVFEALLPQLPPELAGYTWHAVGRLDVDSTGLLLFTNDDKLVAHATSPDAHLPK
RYVATVFSTASDERVEPLRRGMTLDDGPARPAKVNVRDEHTVEVTLTEGRHHQVKRMLGAVGLPVRALHREAVGSVDLEG
IPEGTFRLLTDDEVREGLRYEGRTPFGP
>Mature_267_residues
SRKPDKPKPPPQHKRWEGKEKPDWLSRALARAGVFPQKEAEDAIRAGRVSINGRVAKMALTPVPPGATVKVDGLVVSLEA
PPTRVLAFHKPAGLLSSTERQHRTGTVFEALLPQLPPELAGYTWHAVGRLDVDSTGLLLFTNDDKLVAHATSPDAHLPKR
YVATVFSTASDERVEPLRRGMTLDDGPARPAKVNVRDEHTVEVTLTEGRHHQVKRMLGAVGLPVRALHREAVGSVDLEGI
PEGTFRLLTDDEVREGLRYEGRTPFGP

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=230, Percent_Identity=37.8260869565217, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788510, Length=214, Percent_Identity=35.0467289719626, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790453, Length=231, Percent_Identity=31.6017316017316, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081838, Length=191, Percent_Identity=34.5549738219895, Blast_Score=94, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 29394; Mature: 29263

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKPDKPKPPPQHKRWEGKEKPDWLSRALARAGVFPQKEAEDAIRAGRVSINGRVAKMA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCEEEECCEEEEEE
LTPVPPGATVKVDGLVVSLEAPPTRVLAFHKPAGLLSSTERQHRTGTVFEALLPQLPPEL
ECCCCCCCEEEECCEEEEECCCCCEEEEEECCCCCHHHHHHHHHCHHHHHHHHHHCCHHH
AGYTWHAVGRLDVDSTGLLLFTNDDKLVAHATSPDAHLPKRYVATVFSTASDERVEPLRR
CCEEEEEEEEEEECCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHC
GMTLDDGPARPAKVNVRDEHTVEVTLTEGRHHQVKRMLGAVGLPVRALHREAVGSVDLEG
CCCCCCCCCCCCEEECCCCEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCC
IPEGTFRLLTDDEVREGLRYEGRTPFGP
CCCCCEEEECCHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SRKPDKPKPPPQHKRWEGKEKPDWLSRALARAGVFPQKEAEDAIRAGRVSINGRVAKMA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCEEEECCEEEEEE
LTPVPPGATVKVDGLVVSLEAPPTRVLAFHKPAGLLSSTERQHRTGTVFEALLPQLPPEL
ECCCCCCCEEEECCEEEEECCCCCEEEEEECCCCCHHHHHHHHHCHHHHHHHHHHCCHHH
AGYTWHAVGRLDVDSTGLLLFTNDDKLVAHATSPDAHLPKRYVATVFSTASDERVEPLRR
CCEEEEEEEEEEECCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHC
GMTLDDGPARPAKVNVRDEHTVEVTLTEGRHHQVKRMLGAVGLPVRALHREAVGSVDLEG
CCCCCCCCCCCCEEECCCCEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCC
IPEGTFRLLTDDEVREGLRYEGRTPFGP
CCCCCEEEECCHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]