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The map label for this gene is fixL [H]

Identifier: 108763135

GI number: 108763135

Start: 1061417

End: 1063750

Strand: Direct

Name: fixL [H]

Synonym: MXAN_0931

Alternate gene names: 108763135

Gene position: 1061417-1063750 (Clockwise)

Preceding gene: 108761432

Following gene: 108763588

Centisome position: 11.61

GC content: 69.49

Gene sequence:

>2334_bases
GTGAAACTTCGCACGGAGAGAGCGGGGATGGCGGGAGCGCGGGGCTTCGGGCGAGCGTCGGGGGGCTGCTACATGACGAC
GTTGGATGAACTCACGACGTGCGCGGGCCTTGCCTTGGCGCCACCGTCCTCCACGGGAGCCTGCCTCATCCTCATCAGCA
CCACCACGCCGGCAGCCATCGGCAAGGCCTACCGCCTGGACCAGGGCGAGCACATCATCGGCCGCGGTTCCGACGTCACC
GTGCGCATCGACGACCACGGGGTGTCACGCAAGCACGCGCGCGTCGTTCGTGCGGGCGACGGTGCCTGCCACGTCACCGA
CCTGGACTCCACCAACGGAACGCTGCTCAACGGCGTGCCCGTCAGCACCGCCGAGTTGATGGAAGGGGACCGGCTGCAGA
TCGGCACCGTCACCGTGTTCCGCTTCTCCAAGCGCGAAGTCCTGGAGCAGCGGGAGGAGCAGCTTCGCCAGGCGCTGACC
GCCGCGCGCGTGGGCATCTGGGACTGGAACGCGCAGAGCGGCCGGGTGACGTGGAGCGAGCAGGTGGACCGGCTGCTGGG
GCTTCCCGTGGGCAAGTTGTCCGGCCGCGCCATGGAGCTGTCGGAGGTGGTGCACCCAGCGGACCTGCCGCGCGTGAGCG
AGGTGCTGGGCGCCGCGCTGGAGAAGAAGACGCAGGTGGACGTGGAGTACCGCATCGAGCCGCAGGGGAGCGGCTGGCGG
TGGATTTCCTGCAAGGGTGACGTGCTGTGTGACGCCTCGGGCGCGCCAGCGCGGGTGACGGGCACGGTGATGGACATCAC
CGCGCGCAAGCTGGCCGAACAGGAGCTGCACCGCCAATCGCTCATCTTCGAGAGCATCTACGACGGCGTGGTGATTACCG
ACCTGGGTGGCGGCATCATCGACTGGAACTCCAGCGCCGAGCGCATGTTCGGGCGACAGAAGTCGGAAGCGCTGGGGCAG
ACGCTCTTCAGCGTGCTGCACCCGGATGAGCCCGACCGGCTCACCGGCCTGGTTCTCACCGCGCTGGACAAGCAGGGGCG
CTGGTCCGGAGAGCTGGAGTTCAAGCGCCACGACGGCACCACCTGTTGGTGCGAGTCCGTCGTCGTGCCGCTGCGCGACA
GCGAGGGGCGCGCCATCGCCAACATCATGGTCCACCGCGACACGACGGAGCGGAAGCAGCTCCAGGCGCACCTGGTGGTG
GCGGACCGGCTGGCGTCGGTGGGCACGCTGGGCGCGGGCGTGGCGCACGAAATCAACAACCCGCTGGCCTACATGCTCGT
CAACCTGCATCTCATCCGCGAAGGGCTGGAGCGACTGGAGAGCCAGGCGCCCGCGGCGCCCGTGGCGTCGTTGCAGCAGT
TGGTGCGTGAGACAGCGGAAGGCGCGGAGCGCATCGCCACCATCGTCCGCGACCTGAAGGTCTTCGCGCGCGGTGAGCAG
GAGACGCGGCTGATGCCGGTGGACGTGCGCCGGGCGGTGGAGCTGGCGTGCAAGATGGCGGACAACGTCATCCGCCACCG
GGCGCGGCTGGTGACGGAGTTCGAGCCGGTGGCGCCGGTGGAGGCCAGCGAGTCGCGGCTGTGCCAGGTGTTCCTCAACC
TGCTGCTCAACGCGGCGCAGGCGATTCCGGAGGAGGGCACGCCGGGCGTGGAGCACGAGATTCGCGTGGTCATCCGCGCC
GGAGAGAAGGACCGGGTGGTGGTGGAGGTCCGCGACACGGGCATGGGCATGGGCCAGGAGGTGCTGGGCCGCATCTTCGA
CCCGTTCTTCACCACCAAGCCGGTGGGCGTGGGCACGGGGCTGGGGCTGTCCATCTGCCACGGCATCATCGAATCCATGG
GCGGCTCCATCCAGGCGGAGAGCGAGCCGGGCCGGGGCAGCACCTTCCGCGTGGTGCTGTGCGCCGCCACGCGTGAGCTG
GAGGTGTTACCGCGCCTGTCCGCCACCATGCAGGCCAACGTGCGCGCGCGCATCCTGGTGGTGGATGACGAGCCCAACGT
GACGCTGGCGCTCCAGCGCTCGCTGGCCGCGGACCACGAGGTGTCCACCGCGAACAGCGCCCAGGCCGCGCTGCGGCTGG
TGAGCGACGGCGGCCGTTTCGACCTCATCCTCTGTGATGTGATGATGCCCGGGATGACGGGCATGGACCTGTACCACGAG
CTGGGCCGCTGCGCCCCGGAGCAAGCTGGACGCATGGTGTTCATGACGGGCGGCGCCTTCACGCCGCGCACGGTGTCCTT
CCTGCGTGACGTGCCCAACGCCAAGATTGCCAAGCCGCTGGACCTGATGCAGTTGCGCGAGCTCGTGGGCCGCTCCGCCG
AGGCGGGGCGATGA

Upstream 100 bases:

>100_bases
GTGGGCGTGTCCGCGGGGTGTTCGCTGGCGGAAGGTCTGCTGCCCTGGAGTCGGGGGGGATTGGACGGAGGGGCCCTGGC
ACTGATGGGATTCAGGGGGC

Downstream 100 bases:

>100_bases
GCCACCCGGTGAGTGGCCTCTCTGGAACGGACCCCTCGGAGGTCGCGCCCGCGCCCTCCCTGGCCCTGGTGACGGAGCGC
CCCGCGCCGCTGGAGTTGAC

Product: sensory box histidine kinase/response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 777; Mature: 777

Protein sequence:

>777_residues
MKLRTERAGMAGARGFGRASGGCYMTTLDELTTCAGLALAPPSSTGACLILISTTTPAAIGKAYRLDQGEHIIGRGSDVT
VRIDDHGVSRKHARVVRAGDGACHVTDLDSTNGTLLNGVPVSTAELMEGDRLQIGTVTVFRFSKREVLEQREEQLRQALT
AARVGIWDWNAQSGRVTWSEQVDRLLGLPVGKLSGRAMELSEVVHPADLPRVSEVLGAALEKKTQVDVEYRIEPQGSGWR
WISCKGDVLCDASGAPARVTGTVMDITARKLAEQELHRQSLIFESIYDGVVITDLGGGIIDWNSSAERMFGRQKSEALGQ
TLFSVLHPDEPDRLTGLVLTALDKQGRWSGELEFKRHDGTTCWCESVVVPLRDSEGRAIANIMVHRDTTERKQLQAHLVV
ADRLASVGTLGAGVAHEINNPLAYMLVNLHLIREGLERLESQAPAAPVASLQQLVRETAEGAERIATIVRDLKVFARGEQ
ETRLMPVDVRRAVELACKMADNVIRHRARLVTEFEPVAPVEASESRLCQVFLNLLLNAAQAIPEEGTPGVEHEIRVVIRA
GEKDRVVVEVRDTGMGMGQEVLGRIFDPFFTTKPVGVGTGLGLSICHGIIESMGGSIQAESEPGRGSTFRVVLCAATREL
EVLPRLSATMQANVRARILVVDDEPNVTLALQRSLAADHEVSTANSAQAALRLVSDGGRFDLILCDVMMPGMTGMDLYHE
LGRCAPEQAGRMVFMTGGAFTPRTVSFLRDVPNAKIAKPLDLMQLRELVGRSAEAGR

Sequences:

>Translated_777_residues
MKLRTERAGMAGARGFGRASGGCYMTTLDELTTCAGLALAPPSSTGACLILISTTTPAAIGKAYRLDQGEHIIGRGSDVT
VRIDDHGVSRKHARVVRAGDGACHVTDLDSTNGTLLNGVPVSTAELMEGDRLQIGTVTVFRFSKREVLEQREEQLRQALT
AARVGIWDWNAQSGRVTWSEQVDRLLGLPVGKLSGRAMELSEVVHPADLPRVSEVLGAALEKKTQVDVEYRIEPQGSGWR
WISCKGDVLCDASGAPARVTGTVMDITARKLAEQELHRQSLIFESIYDGVVITDLGGGIIDWNSSAERMFGRQKSEALGQ
TLFSVLHPDEPDRLTGLVLTALDKQGRWSGELEFKRHDGTTCWCESVVVPLRDSEGRAIANIMVHRDTTERKQLQAHLVV
ADRLASVGTLGAGVAHEINNPLAYMLVNLHLIREGLERLESQAPAAPVASLQQLVRETAEGAERIATIVRDLKVFARGEQ
ETRLMPVDVRRAVELACKMADNVIRHRARLVTEFEPVAPVEASESRLCQVFLNLLLNAAQAIPEEGTPGVEHEIRVVIRA
GEKDRVVVEVRDTGMGMGQEVLGRIFDPFFTTKPVGVGTGLGLSICHGIIESMGGSIQAESEPGRGSTFRVVLCAATREL
EVLPRLSATMQANVRARILVVDDEPNVTLALQRSLAADHEVSTANSAQAALRLVSDGGRFDLILCDVMMPGMTGMDLYHE
LGRCAPEQAGRMVFMTGGAFTPRTVSFLRDVPNAKIAKPLDLMQLRELVGRSAEAGR
>Mature_777_residues
MKLRTERAGMAGARGFGRASGGCYMTTLDELTTCAGLALAPPSSTGACLILISTTTPAAIGKAYRLDQGEHIIGRGSDVT
VRIDDHGVSRKHARVVRAGDGACHVTDLDSTNGTLLNGVPVSTAELMEGDRLQIGTVTVFRFSKREVLEQREEQLRQALT
AARVGIWDWNAQSGRVTWSEQVDRLLGLPVGKLSGRAMELSEVVHPADLPRVSEVLGAALEKKTQVDVEYRIEPQGSGWR
WISCKGDVLCDASGAPARVTGTVMDITARKLAEQELHRQSLIFESIYDGVVITDLGGGIIDWNSSAERMFGRQKSEALGQ
TLFSVLHPDEPDRLTGLVLTALDKQGRWSGELEFKRHDGTTCWCESVVVPLRDSEGRAIANIMVHRDTTERKQLQAHLVV
ADRLASVGTLGAGVAHEINNPLAYMLVNLHLIREGLERLESQAPAAPVASLQQLVRETAEGAERIATIVRDLKVFARGEQ
ETRLMPVDVRRAVELACKMADNVIRHRARLVTEFEPVAPVEASESRLCQVFLNLLLNAAQAIPEEGTPGVEHEIRVVIRA
GEKDRVVVEVRDTGMGMGQEVLGRIFDPFFTTKPVGVGTGLGLSICHGIIESMGGSIQAESEPGRGSTFRVVLCAATREL
EVLPRLSATMQANVRARILVVDDEPNVTLALQRSLAADHEVSTANSAQAALRLVSDGGRFDLILCDVMMPGMTGMDLYHE
LGRCAPEQAGRMVFMTGGAFTPRTVSFLRDVPNAKIAKPLDLMQLRELVGRSAEAGR

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=356, Percent_Identity=29.4943820224719, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI1790436, Length=252, Percent_Identity=34.1269841269841, Blast_Score=112, Evalue=9e-26,
Organism=Escherichia coli, GI48994928, Length=430, Percent_Identity=26.9767441860465, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI145693157, Length=231, Percent_Identity=32.9004329004329, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788381, Length=256, Percent_Identity=26.171875, Blast_Score=78, Evalue=3e-15,
Organism=Escherichia coli, GI87081816, Length=379, Percent_Identity=26.9129287598945, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI1788713, Length=483, Percent_Identity=23.3954451345756, Blast_Score=74, Evalue=5e-14,
Organism=Escherichia coli, GI1790300, Length=257, Percent_Identity=30.3501945525292, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 84275; Mature: 84275

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50006 FHA_DOMAIN ; PS50112 PAS ; PS50113 PAC ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRTERAGMAGARGFGRASGGCYMTTLDELTTCAGLALAPPSSTGACLILISTTTPAAI
CCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHCCEEECCCCCCCEEEEEEECCCCHHH
GKAYRLDQGEHIIGRGSDVTVRIDDHGVSRKHARVVRAGDGACHVTDLDSTNGTLLNGVP
HHHHCCCCCCEEEECCCEEEEEECCCCCCCHHHEEEECCCCEEEEEECCCCCCEEEECCC
VSTAELMEGDRLQIGTVTVFRFSKREVLEQREEQLRQALTAARVGIWDWNAQSGRVTWSE
CCHHHHCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCEEHHH
QVDRLLGLPVGKLSGRAMELSEVVHPADLPRVSEVLGAALEKKTQVDVEYRIEPQGSGWR
HHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCEE
WISCKGDVLCDASGAPARVTGTVMDITARKLAEQELHRQSLIFESIYDGVVITDLGGGII
EEEECCCEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEE
DWNSSAERMFGRQKSEALGQTLFSVLHPDEPDRLTGLVLTALDKQGRWSGELEFKRHDGT
ECCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHEEECCCCCCCCCEEEEECCCC
TCWCESVVVPLRDSEGRAIANIMVHRDTTERKQLQAHLVVADRLASVGTLGAGVAHEINN
EEEECCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCC
PLAYMLVNLHLIREGLERLESQAPAAPVASLQQLVRETAEGAERIATIVRDLKVFARGEQ
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ETRLMPVDVRRAVELACKMADNVIRHRARLVTEFEPVAPVEASESRLCQVFLNLLLNAAQ
CCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AIPEEGTPGVEHEIRVVIRAGEKDRVVVEVRDTGMGMGQEVLGRIFDPFFTTKPVGVGTG
HCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHCHHHCCCCCCCCCC
LGLSICHGIIESMGGSIQAESEPGRGSTFRVVLCAATRELEVLPRLSATMQANVRARILV
CHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEE
VDDEPNVTLALQRSLAADHEVSTANSAQAALRLVSDGGRFDLILCDVMMPGMTGMDLYHE
EECCCCEEEEEEHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHH
LGRCAPEQAGRMVFMTGGAFTPRTVSFLRDVPNAKIAKPLDLMQLRELVGRSAEAGR
HHCCCHHHCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKLRTERAGMAGARGFGRASGGCYMTTLDELTTCAGLALAPPSSTGACLILISTTTPAAI
CCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHCCEEECCCCCCCEEEEEEECCCCHHH
GKAYRLDQGEHIIGRGSDVTVRIDDHGVSRKHARVVRAGDGACHVTDLDSTNGTLLNGVP
HHHHCCCCCCEEEECCCEEEEEECCCCCCCHHHEEEECCCCEEEEEECCCCCCEEEECCC
VSTAELMEGDRLQIGTVTVFRFSKREVLEQREEQLRQALTAARVGIWDWNAQSGRVTWSE
CCHHHHCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCEEHHH
QVDRLLGLPVGKLSGRAMELSEVVHPADLPRVSEVLGAALEKKTQVDVEYRIEPQGSGWR
HHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCEE
WISCKGDVLCDASGAPARVTGTVMDITARKLAEQELHRQSLIFESIYDGVVITDLGGGII
EEEECCCEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEE
DWNSSAERMFGRQKSEALGQTLFSVLHPDEPDRLTGLVLTALDKQGRWSGELEFKRHDGT
ECCCCHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHEEECCCCCCCCCEEEEECCCC
TCWCESVVVPLRDSEGRAIANIMVHRDTTERKQLQAHLVVADRLASVGTLGAGVAHEINN
EEEECCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCC
PLAYMLVNLHLIREGLERLESQAPAAPVASLQQLVRETAEGAERIATIVRDLKVFARGEQ
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ETRLMPVDVRRAVELACKMADNVIRHRARLVTEFEPVAPVEASESRLCQVFLNLLLNAAQ
CCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AIPEEGTPGVEHEIRVVIRAGEKDRVVVEVRDTGMGMGQEVLGRIFDPFFTTKPVGVGTG
HCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHCHHHCCCCCCCCCC
LGLSICHGIIESMGGSIQAESEPGRGSTFRVVLCAATRELEVLPRLSATMQANVRARILV
CHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEE
VDDEPNVTLALQRSLAADHEVSTANSAQAALRLVSDGGRFDLILCDVMMPGMTGMDLYHE
EECCCCEEEEEEHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHH
LGRCAPEQAGRMVFMTGGAFTPRTVSFLRDVPNAKIAKPLDLMQLRELVGRSAEAGR
HHCCCHHHCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]