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The map label for this gene is spsK [H]

Identifier: 108763033

GI number: 108763033

Start: 5786681

End: 5787583

Strand: Reverse

Name: spsK [H]

Synonym: MXAN_4612

Alternate gene names: 108763033

Gene position: 5787583-5786681 (Counterclockwise)

Preceding gene: 108762258

Following gene: 108762461

Centisome position: 63.32

GC content: 70.1

Gene sequence:

>903_bases
ATGCGCTTCCTCGTCACGGGGTCCAACGGGTTGGTGGGCAGCCGCGTGTGTTCCTTGCTCCACCAGGGCGGGCACCAGGT
GGTGGGCCTGGGCCGCGGTGCCCGGCGTACGGGCGGAGCGTATGGCTACGCGAGCGTGGACCTCACGCGCGAGGCGGACG
TCGCCGCCGCCGTGGAGACCGCCGCGCCCGAGGTGGTCATCCACTGCGCCTCCATGACGGAGGTGGATGCCTGCGAGAAG
GACCCGGAGGCCGCGTACGCGGGGAATGTCACCGCGGCCGCCGCCGTGGCGCGGAGCGCTCGCAAGGCCGGTGCGCACCT
GGTGCACGTGTCCACGGACTACGTCTTCGACGGCGAGGCCGGGCCCTATTCCGAGGACGCGATTCCGAATCCTCGCGGCG
TGTACTCAGTGACGAAGCACATGGGGGAGCAAGCCGCGCGCATGCTCGCGCCCGGCTGCGCCATTGCTCGCACCGCCGTG
GTGTATGGCTGGCCTCCGGTGGAGGGCCGGCTCAACTTCGGTGCCTGGCTGGTGACCGCGCTGGAGAAGGGGCAGCAGGT
GCGCCTCTTCGAGGACCAGATTGTGTCGCCCAGCTTCGCGGACAACGTGGCCGCCATGCTGGTGGAGCTGGGGACGCGGC
GGCTGGGTGGCGTGTGGAACACTTGCGGCGGAACGGTCATCGACCGGGTGGGCTTTGGCCGCGCGCTGTGCGAGGTGTTC
GGGTTCGATGCGAAGCTCATCGTCCCCACCCGCATGGCGGAGCTGAAGCTCGCCAGCCCCCGCCCGCTCAAGAGTGGTCT
GCGCACCGACAAGGTGCGTGCCGAACTTTCCGTTCCTCCCTTGGCGCTCGCGGAGTCCTTGTCGCGCTTCCATGCGACCT
GGCTCGCGGCTCGGGGACACTGA

Upstream 100 bases:

>100_bases
CGTGGACCACCCTGCGTGGTGGCAGCGCGTCACGAGTGGCGCCTATCGCCAGTACTTCGAAACGCAGTACCGAACGCGCC
TGCAGGGGAGGGCCTGAGCC

Downstream 100 bases:

>100_bases
CGTGCCGAGGTGGACATGAAGGGCATCATCCTCGCCGGTGGCTCGGGGACGCGGCTGTACCCACTGACGCGCGTGGTCAG
CAAGCAGTTGCTGCCCGTGC

Product: putative dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MRFLVTGSNGLVGSRVCSLLHQGGHQVVGLGRGARRTGGAYGYASVDLTREADVAAAVETAAPEVVIHCASMTEVDACEK
DPEAAYAGNVTAAAAVARSARKAGAHLVHVSTDYVFDGEAGPYSEDAIPNPRGVYSVTKHMGEQAARMLAPGCAIARTAV
VYGWPPVEGRLNFGAWLVTALEKGQQVRLFEDQIVSPSFADNVAAMLVELGTRRLGGVWNTCGGTVIDRVGFGRALCEVF
GFDAKLIVPTRMAELKLASPRPLKSGLRTDKVRAELSVPPLALAESLSRFHATWLAARGH

Sequences:

>Translated_300_residues
MRFLVTGSNGLVGSRVCSLLHQGGHQVVGLGRGARRTGGAYGYASVDLTREADVAAAVETAAPEVVIHCASMTEVDACEK
DPEAAYAGNVTAAAAVARSARKAGAHLVHVSTDYVFDGEAGPYSEDAIPNPRGVYSVTKHMGEQAARMLAPGCAIARTAV
VYGWPPVEGRLNFGAWLVTALEKGQQVRLFEDQIVSPSFADNVAAMLVELGTRRLGGVWNTCGGTVIDRVGFGRALCEVF
GFDAKLIVPTRMAELKLASPRPLKSGLRTDKVRAELSVPPLALAESLSRFHATWLAARGH
>Mature_300_residues
MRFLVTGSNGLVGSRVCSLLHQGGHQVVGLGRGARRTGGAYGYASVDLTREADVAAAVETAAPEVVIHCASMTEVDACEK
DPEAAYAGNVTAAAAVARSARKAGAHLVHVSTDYVFDGEAGPYSEDAIPNPRGVYSVTKHMGEQAARMLAPGCAIARTAV
VYGWPPVEGRLNFGAWLVTALEKGQQVRLFEDQIVSPSFADNVAAMLVELGTRRLGGVWNTCGGTVIDRVGFGRALCEVF
GFDAKLIVPTRMAELKLASPRPLKSGLRTDKVRAELSVPPLALAESLSRFHATWLAARGH

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=281, Percent_Identity=29.5373665480427, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI11034825, Length=281, Percent_Identity=29.5373665480427, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1788352, Length=293, Percent_Identity=26.2798634812287, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 31667; Mature: 31667

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLVTGSNGLVGSRVCSLLHQGGHQVVGLGRGARRTGGAYGYASVDLTREADVAAAVET
CEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHH
AAPEVVIHCASMTEVDACEKDPEAAYAGNVTAAAAVARSARKAGAHLVHVSTDYVFDGEA
CCCEEEEEECCCCHHHHCCCCCCCHHCCCHHHHHHHHHHHHHCCCEEEEEEECEEECCCC
GPYSEDAIPNPRGVYSVTKHMGEQAARMLAPGCAIARTAVVYGWPPVEGRLNFGAWLVTA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHEEEEECCCCCCCCCCHHHHHHHH
LEKGQQVRLFEDQIVSPSFADNVAAMLVELGTRRLGGVWNTCGGTVIDRVGFGRALCEVF
HHCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHCHHHHHHHHH
GFDAKLIVPTRMAELKLASPRPLKSGLRTDKVRAELSVPPLALAESLSRFHATWLAARGH
CCCCEEEECCCHHHEECCCCCHHHHCCCHHHHHEECCCCHHHHHHHHHHHHHHHHEECCC
>Mature Secondary Structure
MRFLVTGSNGLVGSRVCSLLHQGGHQVVGLGRGARRTGGAYGYASVDLTREADVAAAVET
CEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHH
AAPEVVIHCASMTEVDACEKDPEAAYAGNVTAAAAVARSARKAGAHLVHVSTDYVFDGEA
CCCEEEEEECCCCHHHHCCCCCCCHHCCCHHHHHHHHHHHHHCCCEEEEEEECEEECCCC
GPYSEDAIPNPRGVYSVTKHMGEQAARMLAPGCAIARTAVVYGWPPVEGRLNFGAWLVTA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHEEEEECCCCCCCCCCHHHHHHHH
LEKGQQVRLFEDQIVSPSFADNVAAMLVELGTRRLGGVWNTCGGTVIDRVGFGRALCEVF
HHCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHCHHHHHHHHH
GFDAKLIVPTRMAELKLASPRPLKSGLRTDKVRAELSVPPLALAESLSRFHATWLAARGH
CCCCEEEECCCHHHEECCCCCHHHHCCCHHHHHEECCCCHHHHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]