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The map label for this gene is rfbB [H]

Identifier: 108762258

GI number: 108762258

Start: 5787587

End: 5788612

Strand: Reverse

Name: rfbB [H]

Synonym: MXAN_4613

Alternate gene names: 108762258

Gene position: 5788612-5787587 (Counterclockwise)

Preceding gene: 108762403

Following gene: 108763033

Centisome position: 63.33

GC content: 67.06

Gene sequence:

>1026_bases
ATGAACGTACTGGTGACAGGTGGCTGTGGCTTCATCGGGTCCAACCTCGTGAAGTATCTGCGCCGTGAGCGGCCTGCCTG
GACGGTGGTCAACCTCGACAAGCTCACGTACGCCGGCAACCTGGAGAGCCTGTCCGAGCTGGAGGGCGACCCCCAGCACG
TCTTCATCCGGGGCGACATCGGCAATCGCGAGCTGGTGGAGCACCTGATGTCCGTGCACGCCATCGACGCGGTGATGCAC
CTGGCGGCGGAGAGCCACGTCGACCGCTCCATCCTCGGGCCGGAGGTGTTCGTCACCACCAACGTGCTGGGCACCCAGCA
GCTCCTGGAGGCCAGCCGCGCCCGCGGCGTGAGGCGCTTCCTCATGGTGTCCACCGACGAGGTCTACGGCTCGCTGGGGC
CCACGGGCGCCTTCACCGAGTCCTCGCCGTTGCAGCCCTCCAGCCCGTACTCGGCCAGCAAGACGAGCTCCGACCTGGTG
GCGCTCGCGTATCACCACACGTTCAACCTGGACGTCGTCGTCACGCGCTGCTCGAACAACTACGGCCGCTACCAGTTCCC
GGAGAAGTTGATTCCGTTGATGGTGGTGAACGCGCTGCACGACAAGCCGCTGCCGGTGTACGGCGACGGCGGCAACGTGC
GCGACTGGCTCCACGTGGAGGACCACTGCCATGCGCTGCTATTGGCGCTGGAGAAGGGCCGGGCCGGCGAGGTCTACAAC
ATCGGCGGCGGGGCGGAGCGGCGGAACATCGACATCGTGAAGGCGATTCTGGGCCTCGTGGGCAAGCCGGAGTCGCTCAT
CCAGTACGTGAAGGACCGGCCGGGGCATGACCGGCGCTACGCCATCGACCCGTCGAAGATTCGCGCGGAGCTGGGCTGGA
CGCCCGCGCACACCTTCGAGCAAGGCCTGGCGGAGACGGTGCGCTGGTTCGTGGACCACCCTGCGTGGTGGCAGCGCGTC
ACGAGTGGCGCCTATCGCCAGTACTTCGAAACGCAGTACCGAACGCGCCTGCAGGGGAGGGCCTGA

Upstream 100 bases:

>100_bases
TGCTGGGCCGCCCACAAGCCTGGCTTCCCTCGCGCCTTGACGGTCGTGGACCCCTTCGATACGCAGGCGCTCGCTCCGAG
CGCATGAGGTCCACGGGACA

Downstream 100 bases:

>100_bases
GCCATGCGCTTCCTCGTCACGGGGTCCAACGGGTTGGTGGGCAGCCGCGTGTGTTCCTTGCTCCACCAGGGCGGGCACCA
GGTGGTGGGCCTGGGCCGCG

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MNVLVTGGCGFIGSNLVKYLRRERPAWTVVNLDKLTYAGNLESLSELEGDPQHVFIRGDIGNRELVEHLMSVHAIDAVMH
LAAESHVDRSILGPEVFVTTNVLGTQQLLEASRARGVRRFLMVSTDEVYGSLGPTGAFTESSPLQPSSPYSASKTSSDLV
ALAYHHTFNLDVVVTRCSNNYGRYQFPEKLIPLMVVNALHDKPLPVYGDGGNVRDWLHVEDHCHALLLALEKGRAGEVYN
IGGGAERRNIDIVKAILGLVGKPESLIQYVKDRPGHDRRYAIDPSKIRAELGWTPAHTFEQGLAETVRWFVDHPAWWQRV
TSGAYRQYFETQYRTRLQGRA

Sequences:

>Translated_341_residues
MNVLVTGGCGFIGSNLVKYLRRERPAWTVVNLDKLTYAGNLESLSELEGDPQHVFIRGDIGNRELVEHLMSVHAIDAVMH
LAAESHVDRSILGPEVFVTTNVLGTQQLLEASRARGVRRFLMVSTDEVYGSLGPTGAFTESSPLQPSSPYSASKTSSDLV
ALAYHHTFNLDVVVTRCSNNYGRYQFPEKLIPLMVVNALHDKPLPVYGDGGNVRDWLHVEDHCHALLLALEKGRAGEVYN
IGGGAERRNIDIVKAILGLVGKPESLIQYVKDRPGHDRRYAIDPSKIRAELGWTPAHTFEQGLAETVRWFVDHPAWWQRV
TSGAYRQYFETQYRTRLQGRA
>Mature_341_residues
MNVLVTGGCGFIGSNLVKYLRRERPAWTVVNLDKLTYAGNLESLSELEGDPQHVFIRGDIGNRELVEHLMSVHAIDAVMH
LAAESHVDRSILGPEVFVTTNVLGTQQLLEASRARGVRRFLMVSTDEVYGSLGPTGAFTESSPLQPSSPYSASKTSSDLV
ALAYHHTFNLDVVVTRCSNNYGRYQFPEKLIPLMVVNALHDKPLPVYGDGGNVRDWLHVEDHCHALLLALEKGRAGEVYN
IGGGAERRNIDIVKAILGLVGKPESLIQYVKDRPGHDRRYAIDPSKIRAELGWTPAHTFEQGLAETVRWFVDHPAWWQRV
TSGAYRQYFETQYRTRLQGRA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=324, Percent_Identity=37.037037037037, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI42516563, Length=314, Percent_Identity=26.7515923566879, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI56237023, Length=349, Percent_Identity=27.2206303724928, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI56118217, Length=349, Percent_Identity=27.2206303724928, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI189083684, Length=349, Percent_Identity=27.2206303724928, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI48994969, Length=346, Percent_Identity=51.1560693641619, Blast_Score=338, Evalue=2e-94,
Organism=Escherichia coli, GI1788353, Length=361, Percent_Identity=49.584487534626, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1786974, Length=337, Percent_Identity=30.5637982195846, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1788366, Length=347, Percent_Identity=24.207492795389, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=321, Percent_Identity=37.0716510903427, Blast_Score=204, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=319, Percent_Identity=34.7962382445141, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17539532, Length=315, Percent_Identity=26.031746031746, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71982035, Length=354, Percent_Identity=26.5536723163842, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=356, Percent_Identity=26.4044943820225, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17507723, Length=337, Percent_Identity=24.0356083086053, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=352, Percent_Identity=25.2840909090909, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356223, Length=335, Percent_Identity=27.4626865671642, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19923002, Length=346, Percent_Identity=29.1907514450867, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24158427, Length=342, Percent_Identity=23.3918128654971, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 38089; Mature: 38089

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLVTGGCGFIGSNLVKYLRRERPAWTVVNLDKLTYAGNLESLSELEGDPQHVFIRGDI
CCEEEECCCHHHHHHHHHHHHHCCCCEEEEEHHHEEECCCHHHHHHCCCCCCEEEEECCC
GNRELVEHLMSVHAIDAVMHLAAESHVDRSILGPEVFVTTNVLGTQQLLEASRARGVRRF
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHH
LMVSTDEVYGSLGPTGAFTESSPLQPSSPYSASKTSSDLVALAYHHTFNLDVVVTRCSNN
HEEECHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECEEEEEEECCCC
YGRYQFPEKLIPLMVVNALHDKPLPVYGDGGNVRDWLHVEDHCHALLLALEKGRAGEVYN
CCCEECHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEHHHHHHHHHHHHCCCCCCCEEE
IGGGAERRNIDIVKAILGLVGKPESLIQYVKDRPGHDRRYAIDPSKIRAELGWTPAHTFE
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEECHHHHHHHCCCCCHHHHH
QGLAETVRWFVDHPAWWQRVTSGAYRQYFETQYRTRLQGRA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNVLVTGGCGFIGSNLVKYLRRERPAWTVVNLDKLTYAGNLESLSELEGDPQHVFIRGDI
CCEEEECCCHHHHHHHHHHHHHCCCCEEEEEHHHEEECCCHHHHHHCCCCCCEEEEECCC
GNRELVEHLMSVHAIDAVMHLAAESHVDRSILGPEVFVTTNVLGTQQLLEASRARGVRRF
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHH
LMVSTDEVYGSLGPTGAFTESSPLQPSSPYSASKTSSDLVALAYHHTFNLDVVVTRCSNN
HEEECHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECEEEEEEECCCC
YGRYQFPEKLIPLMVVNALHDKPLPVYGDGGNVRDWLHVEDHCHALLLALEKGRAGEVYN
CCCEECHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEHHHHHHHHHHHHCCCCCCCEEE
IGGGAERRNIDIVKAILGLVGKPESLIQYVKDRPGHDRRYAIDPSKIRAELGWTPAHTFE
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEECHHHHHHHCCCCCHHHHH
QGLAETVRWFVDHPAWWQRVTSGAYRQYFETQYRTRLQGRA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]