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The map label for this gene is gspE [C]

Identifier: 108762982

GI number: 108762982

Start: 3641054

End: 3642841

Strand: Direct

Name: gspE [C]

Synonym: MXAN_3107

Alternate gene names: 108762982

Gene position: 3641054-3642841 (Clockwise)

Preceding gene: 108762925

Following gene: 108757246

Centisome position: 39.84

GC content: 63.48

Gene sequence:

>1788_bases
ATGTTTCTCATCACCCTCGCTGAAAAGGGCGGCGGGACCGAGCAGCGCGAGTATCACAAGAATGAAGTCACCATCGGCCG
TCTGCCGGGCAATGACATCATCCTCGCGAAGGGCAACGTCTCCAAGTACCACTCGCGAATCGTTGCCAAGGACGGGAAGT
TCATCATCGTGGACATGAAGTCCACGAATGGCACGTTCGTGAACGGCAAGAAGATTGCCGCGCCCCAGGTTCTCAAGCCG
ACCGACCAGGTCTACATCGGCGACTACATCCTCAACGTCGAGGCGCTCGAGGACGAGGGCCCGGTGATGACCCGCGCGGG
TCAGCCCGAAGAGGAGTACTACGACGAGCAGGGCGAGGAGCCCTACGAGGACGAGGAAGGGGCTTACGAAGAAGACGAGC
CCTACGAGGAGGAGGAAGAAGAAGCGCCGCCTCCCGCGCCCGCTCCCAAGGGCATGCCGGCGTCGCTGGCGTCCGCCCTG
GCGAAGAACAAGCGCAAGGTGGATCCGCGTCAGGAGCGCTACACCCGGCTCCAGAAGGAAATCCATGACCGGCTCATCGA
GTACCTCGATCTGCGCCGCATGGACATGGACCGGCTCGGCGACGACGAGCTGTGGCGCCGCACCGAGAAGGCCATCCGCG
ACATCATCGACCAGATGGAAGCGGACGGAGAGCTTCCGGAGGACGTGGACCGCGAGGAGCTGCTCACGGACGTCATCAAC
GAGGCGCTGGGGCTCGGGCCCCTCGAGGCGTTCCTCGCGTCGGATGACATCAGCGAGATCATGGTGAACCACGCCAACCA
GATCTACATCGAGCGCAAGGGCAAGCTGACCCTGTCGGAGAAGACGTTCTCCTCCAACCAGGCGGTGCTCGGCGTCATCG
AGCGCATCGTGGCGCCCATTGGCCGCCGCATCGACGAGTCCAGCCCGCTGGTGGACGCGCGCCTCAAGGACGGCAGCCGC
GTCAACGCCATCATCCCTCCGCTGGCGCTGAAGGGTCCCTGCATCACCATCCGCAAGTTCAAGAAGGACTCGCTGAAGAT
CGCGGACCTCATCAAGTACAAGACCGTCACGGCGCAGATGGCCGAGTTCCTGGAGATGTGCGTCAAGGCCCGGCGCAACA
TCGTCATCTCCGGCGGCACCGGCTCCGGGAAGACGACGACGCTGAACATCATCAGCTCCTTCATCCCGGAGGGTGAGCGC
ATCATCACCGTGGAGGACGCCGCCGAGCTGCAACTGCCGCAGGACCACTGGGTGCAGCTGGAGAGCCGGCCGCCCAACCT
GGAAGGCAAGGGCGCCATCACCATCCGCGAGCTGGTGAAGAACTGCCTGCGCATGCGGCCCGACCGCATCGTCGTGGGTG
AGTGCCGCTCCGGTGAGACGCTGGACATGCTCCAGGCCATGAACACCGGCCACGACGGTTCGCTCACCACGCTCCATGCG
AATACGCCGCGAGACGCCATCGCCCGCTTGGAGACGATGGTGCTCATGTCCGGCATGGACCTGCCGGTGAAGGCCATCCG
TGAGCAGATCGCCAGCGCGGTGCACATGATCGTGCAGCAGACGCGCTTCTCCGACGGCACGCGGAAGATCTGCTACATCA
CCGAGGTGTCCGGCATGGAGGTCGATATCGTGACCCTCCAGGACATCTTCTATTTCAAACAGGATGGCTTCACGGAGGAC
CACAAGGTCCGTGGCCGCTACGTCGCCTCCGGCTTCGTGCCGAAGTTCTACGACGAGCTGCAGCGTAAGGGCATCCCCGT
CAACATGAGCATCTTCCGCGAGGACTGA

Upstream 100 bases:

>100_bases
CCCTTCCGGGGGCGGATGCCGGCCCGTCGCCTTGCGGGCAAGCATGCAACCCGGTTCTCACGCTTTGAGGACGAAGGGGG
GCTTGCTAGCATCCGTCCCC

Downstream 100 bases:

>100_bases
CGCGCCCATGGCCACCCTGGTCGTCCGTCACCCCGACGGCACTGAGAACGAATTCGCCATCACGGGCGAGCTGAAGATTG
GCCGCCAGCAGGGCAGTGAC

Product: type II/IV secretion system protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
MFLITLAEKGGGTEQREYHKNEVTIGRLPGNDIILAKGNVSKYHSRIVAKDGKFIIVDMKSTNGTFVNGKKIAAPQVLKP
TDQVYIGDYILNVEALEDEGPVMTRAGQPEEEYYDEQGEEPYEDEEGAYEEDEPYEEEEEEAPPPAPAPKGMPASLASAL
AKNKRKVDPRQERYTRLQKEIHDRLIEYLDLRRMDMDRLGDDELWRRTEKAIRDIIDQMEADGELPEDVDREELLTDVIN
EALGLGPLEAFLASDDISEIMVNHANQIYIERKGKLTLSEKTFSSNQAVLGVIERIVAPIGRRIDESSPLVDARLKDGSR
VNAIIPPLALKGPCITIRKFKKDSLKIADLIKYKTVTAQMAEFLEMCVKARRNIVISGGTGSGKTTTLNIISSFIPEGER
IITVEDAAELQLPQDHWVQLESRPPNLEGKGAITIRELVKNCLRMRPDRIVVGECRSGETLDMLQAMNTGHDGSLTTLHA
NTPRDAIARLETMVLMSGMDLPVKAIREQIASAVHMIVQQTRFSDGTRKICYITEVSGMEVDIVTLQDIFYFKQDGFTED
HKVRGRYVASGFVPKFYDELQRKGIPVNMSIFRED

Sequences:

>Translated_595_residues
MFLITLAEKGGGTEQREYHKNEVTIGRLPGNDIILAKGNVSKYHSRIVAKDGKFIIVDMKSTNGTFVNGKKIAAPQVLKP
TDQVYIGDYILNVEALEDEGPVMTRAGQPEEEYYDEQGEEPYEDEEGAYEEDEPYEEEEEEAPPPAPAPKGMPASLASAL
AKNKRKVDPRQERYTRLQKEIHDRLIEYLDLRRMDMDRLGDDELWRRTEKAIRDIIDQMEADGELPEDVDREELLTDVIN
EALGLGPLEAFLASDDISEIMVNHANQIYIERKGKLTLSEKTFSSNQAVLGVIERIVAPIGRRIDESSPLVDARLKDGSR
VNAIIPPLALKGPCITIRKFKKDSLKIADLIKYKTVTAQMAEFLEMCVKARRNIVISGGTGSGKTTTLNIISSFIPEGER
IITVEDAAELQLPQDHWVQLESRPPNLEGKGAITIRELVKNCLRMRPDRIVVGECRSGETLDMLQAMNTGHDGSLTTLHA
NTPRDAIARLETMVLMSGMDLPVKAIREQIASAVHMIVQQTRFSDGTRKICYITEVSGMEVDIVTLQDIFYFKQDGFTED
HKVRGRYVASGFVPKFYDELQRKGIPVNMSIFRED
>Mature_595_residues
MFLITLAEKGGGTEQREYHKNEVTIGRLPGNDIILAKGNVSKYHSRIVAKDGKFIIVDMKSTNGTFVNGKKIAAPQVLKP
TDQVYIGDYILNVEALEDEGPVMTRAGQPEEEYYDEQGEEPYEDEEGAYEEDEPYEEEEEEAPPPAPAPKGMPASLASAL
AKNKRKVDPRQERYTRLQKEIHDRLIEYLDLRRMDMDRLGDDELWRRTEKAIRDIIDQMEADGELPEDVDREELLTDVIN
EALGLGPLEAFLASDDISEIMVNHANQIYIERKGKLTLSEKTFSSNQAVLGVIERIVAPIGRRIDESSPLVDARLKDGSR
VNAIIPPLALKGPCITIRKFKKDSLKIADLIKYKTVTAQMAEFLEMCVKARRNIVISGGTGSGKTTTLNIISSFIPEGER
IITVEDAAELQLPQDHWVQLESRPPNLEGKGAITIRELVKNCLRMRPDRIVVGECRSGETLDMLQAMNTGHDGSLTTLHA
NTPRDAIARLETMVLMSGMDLPVKAIREQIASAVHMIVQQTRFSDGTRKICYITEVSGMEVDIVTLQDIFYFKQDGFTED
HKVRGRYVASGFVPKFYDELQRKGIPVNMSIFRED

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=168, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1786296, Length=175, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 66884; Mature: 66884

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50006 FHA_DOMAIN ; PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLITLAEKGGGTEQREYHKNEVTIGRLPGNDIILAKGNVSKYHSRIVAKDGKFIIVDMK
CEEEEEECCCCCCCHHHHCCCCEEEEECCCCCEEEECCCHHHHHHHHEECCCCEEEEEEE
STNGTFVNGKKIAAPQVLKPTDQVYIGDYILNVEALEDEGPVMTRAGQPEEEYYDEQGEE
CCCCCEECCCEECCCCCCCCCCCEEEEEEEEEEEEECCCCCEEECCCCCHHHHHHCCCCC
PYEDEEGAYEEDEPYEEEEEEAPPPAPAPKGMPASLASALAKNKRKVDPRQERYTRLQKE
CCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
IHDRLIEYLDLRRMDMDRLGDDELWRRTEKAIRDIIDQMEADGELPEDVDREELLTDVIN
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
EALGLGPLEAFLASDDISEIMVNHANQIYIERKGKLTLSEKTFSSNQAVLGVIERIVAPI
HHHCCCHHHHHHHCCCHHHHHHCCCCEEEEEECCCEEEECCCCCCCHHHHHHHHHHHHHH
GRRIDESSPLVDARLKDGSRVNAIIPPLALKGPCITIRKFKKDSLKIADLIKYKTVTAQM
HHCCCCCCCCEEEECCCCCEEEEECCCCCCCCCEEEHHHHCCCCHHHHHHHHHHHHHHHH
AEFLEMCVKARRNIVISGGTGSGKTTTLNIISSFIPEGERIITVEDAAELQLPQDHWVQL
HHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEECCHHCCCCHHHCEEE
ESRPPNLEGKGAITIRELVKNCLRMRPDRIVVGECRSGETLDMLQAMNTGHDGSLTTLHA
CCCCCCCCCCCCEEHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEC
NTPRDAIARLETMVLMSGMDLPVKAIREQIASAVHMIVQQTRFSDGTRKICYITEVSGME
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCE
VDIVTLQDIFYFKQDGFTEDHKVRGRYVASGFVPKFYDELQRKGIPVNMSIFRED
EEEEEHHHHHHHHCCCCCCCHHHCCEEEECCCCHHHHHHHHHCCCCCEEEEEECC
>Mature Secondary Structure
MFLITLAEKGGGTEQREYHKNEVTIGRLPGNDIILAKGNVSKYHSRIVAKDGKFIIVDMK
CEEEEEECCCCCCCHHHHCCCCEEEEECCCCCEEEECCCHHHHHHHHEECCCCEEEEEEE
STNGTFVNGKKIAAPQVLKPTDQVYIGDYILNVEALEDEGPVMTRAGQPEEEYYDEQGEE
CCCCCEECCCEECCCCCCCCCCCEEEEEEEEEEEEECCCCCEEECCCCCHHHHHHCCCCC
PYEDEEGAYEEDEPYEEEEEEAPPPAPAPKGMPASLASALAKNKRKVDPRQERYTRLQKE
CCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
IHDRLIEYLDLRRMDMDRLGDDELWRRTEKAIRDIIDQMEADGELPEDVDREELLTDVIN
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
EALGLGPLEAFLASDDISEIMVNHANQIYIERKGKLTLSEKTFSSNQAVLGVIERIVAPI
HHHCCCHHHHHHHCCCHHHHHHCCCCEEEEEECCCEEEECCCCCCCHHHHHHHHHHHHHH
GRRIDESSPLVDARLKDGSRVNAIIPPLALKGPCITIRKFKKDSLKIADLIKYKTVTAQM
HHCCCCCCCCEEEECCCCCEEEEECCCCCCCCCEEEHHHHCCCCHHHHHHHHHHHHHHHH
AEFLEMCVKARRNIVISGGTGSGKTTTLNIISSFIPEGERIITVEDAAELQLPQDHWVQL
HHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEECCHHCCCCHHHCEEE
ESRPPNLEGKGAITIRELVKNCLRMRPDRIVVGECRSGETLDMLQAMNTGHDGSLTTLHA
CCCCCCCCCCCCEEHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEC
NTPRDAIARLETMVLMSGMDLPVKAIREQIASAVHMIVQQTRFSDGTRKICYITEVSGME
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCE
VDIVTLQDIFYFKQDGFTEDHKVRGRYVASGFVPKFYDELQRKGIPVNMSIFRED
EEEEEHHHHHHHHCCCCCCCHHHCCEEEECCCCHHHHHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]