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The map label for this gene is rlmI [H]

Identifier: 108762676

GI number: 108762676

Start: 2323133

End: 2324329

Strand: Direct

Name: rlmI [H]

Synonym: MXAN_1988

Alternate gene names: 108762676

Gene position: 2323133-2324329 (Clockwise)

Preceding gene: 108762988

Following gene: 108757806

Centisome position: 25.42

GC content: 72.85

Gene sequence:

>1197_bases
GTGAGCCTGATGCCCCCCCATGCAGCCCTGCCCGTCGCGCGCGTCACCCCCAAGGGGGCGCGCTCGCTGCGGCACTTCAA
TCCCTGGGTGTACCGCACCGAAATCGCCGCCCCGCCCGACGTGAAGGGCGCCGGCGCCGTGGTGCTGGTGGTGGACTCCC
AGGGCAATCCCATTGGCCAGGCGCTCTATGCCCGCCGCTCGCCCCTGGCGCTGCGCCTGCTGACGCGCAAGGGGCCCGCC
GAGGAGCCGGTGGACGACGCCTTCTTCCGCCGCCGCCTGGAGGCCGCCCTGGCGCGCCGGGCGTACCTGTCCGGCCGTGA
CGGGCTGCGGCTGGTGCACGGCGAGGCGGACCAGCTCCCGGGCCTCTTCGTGGACCGCTACGGAAAGGGCCTCACGCTCC
AGACGCTCTCCGAGGGCATGGACGCGCGCAAGGAGACGCTGGCGAAGATGCTGGTGGAGCTCACCGGCGCCACCCACGTC
ATGTGCCGGGACGACGCCTCCGGCCGTGACTTCGAAGGCCTGCCCCGCGAGGCGCGCCTGCTGCACGGTGAGGGCGCCGC
GCGCTTCACCTACCACGAGGGGGAGAACCGCTTCGAGGTCGACCTCCAGGGCGACATGAAGACGGGCGCCTTCCTGGACC
AGGTGGACAACCACCTGCGGGCCGGGGAGCTGGCGCGCGGCGACGCGCTGGACCTCTTCAGCTACCACGGCGGCTTCGCG
CTCTCGCTGTCACGCACCTGCACGTCCGTGCTGGCGGTGGAGCAGGACGAGAAGGCCGCCGCGCGCGCCAAGGCCAACGC
CGAGGCCAACGGCCGCGCCAACGTCACCGTGGAGAACGCCAACGCCTTCGATGTGCTGCGCCGCTTCGACACCAGCGGGC
GCCGCTTCGACACCGTGGTGTTGGACCCGCCCGGACTGGCCAAGCGCCGCGAGGGCCTGGCCACCGCGCTGCGCGCCTAC
CACGAGCTGAACCTGCGCGCCTTCCGCTGCCTCAAGCCGGACGGGTTGCTCGTCACCTGCTCCTGCTCCGGCAAGCTGGA
CCGCGCCGCCTTCGAGGAGATGGTGCTGGCGGCCGCCGCGGATGCGAAGCGGCCGGTGCAGATTCTGGAGCGGCGGGGCG
CGGGGTTGGACCACCCGGTGCTGGCCGGGCTGCCGGAGACGGAGTACCTGAAGGCCCTCTACGTGCGCGCCCTCTAG

Upstream 100 bases:

>100_bases
CGCCCGAGGAACTTCGCGAGCAGATCCGCGTGACGTTGCTGCACGAAATCGGGCACCTTCGCGGCGAGGACGACGAAGAA
CTGGCCGCTCGCGGCCTGGA

Downstream 100 bases:

>100_bases
CGAGGGGAGGGGGCTGGGGCCTATGGAATCCCCAGCTCCTTGCGCAGCCGGCCCACGATTTTGAAGTACTCCGTTCGTGG
GAAGGGGACGTTGAGGATGT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSLMPPHAALPVARVTPKGARSLRHFNPWVYRTEIAAPPDVKGAGAVVLVVDSQGNPIGQALYARRSPLALRLLTRKGPA
EEPVDDAFFRRRLEAALARRAYLSGRDGLRLVHGEADQLPGLFVDRYGKGLTLQTLSEGMDARKETLAKMLVELTGATHV
MCRDDASGRDFEGLPREARLLHGEGAARFTYHEGENRFEVDLQGDMKTGAFLDQVDNHLRAGELARGDALDLFSYHGGFA
LSLSRTCTSVLAVEQDEKAAARAKANAEANGRANVTVENANAFDVLRRFDTSGRRFDTVVLDPPGLAKRREGLATALRAY
HELNLRAFRCLKPDGLLVTCSCSGKLDRAAFEEMVLAAAADAKRPVQILERRGAGLDHPVLAGLPETEYLKALYVRAL

Sequences:

>Translated_398_residues
MSLMPPHAALPVARVTPKGARSLRHFNPWVYRTEIAAPPDVKGAGAVVLVVDSQGNPIGQALYARRSPLALRLLTRKGPA
EEPVDDAFFRRRLEAALARRAYLSGRDGLRLVHGEADQLPGLFVDRYGKGLTLQTLSEGMDARKETLAKMLVELTGATHV
MCRDDASGRDFEGLPREARLLHGEGAARFTYHEGENRFEVDLQGDMKTGAFLDQVDNHLRAGELARGDALDLFSYHGGFA
LSLSRTCTSVLAVEQDEKAAARAKANAEANGRANVTVENANAFDVLRRFDTSGRRFDTVVLDPPGLAKRREGLATALRAY
HELNLRAFRCLKPDGLLVTCSCSGKLDRAAFEEMVLAAAADAKRPVQILERRGAGLDHPVLAGLPETEYLKALYVRAL
>Mature_397_residues
SLMPPHAALPVARVTPKGARSLRHFNPWVYRTEIAAPPDVKGAGAVVLVVDSQGNPIGQALYARRSPLALRLLTRKGPAE
EPVDDAFFRRRLEAALARRAYLSGRDGLRLVHGEADQLPGLFVDRYGKGLTLQTLSEGMDARKETLAKMLVELTGATHVM
CRDDASGRDFEGLPREARLLHGEGAARFTYHEGENRFEVDLQGDMKTGAFLDQVDNHLRAGELARGDALDLFSYHGGFAL
SLSRTCTSVLAVEQDEKAAARAKANAEANGRANVTVENANAFDVLRRFDTSGRRFDTVVLDPPGLAKRREGLATALRAYH
ELNLRAFRCLKPDGLLVTCSCSGKLDRAAFEEMVLAAAADAKRPVQILERRGAGLDHPVLAGLPETEYLKALYVRAL

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=398, Percent_Identity=34.1708542713568, Blast_Score=204, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43445; Mature: 43314

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLMPPHAALPVARVTPKGARSLRHFNPWVYRTEIAAPPDVKGAGAVVLVVDSQGNPIGQ
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCHHH
ALYARRSPLALRLLTRKGPAEEPVDDAFFRRRLEAALARRAYLSGRDGLRLVHGEADQLP
HHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCC
GLFVDRYGKGLTLQTLSEGMDARKETLAKMLVELTGATHVMCRDDASGRDFEGLPREARL
CHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHE
LHGEGAARFTYHEGENRFEVDLQGDMKTGAFLDQVDNHLRAGELARGDALDLFSYHGGFA
EECCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEE
LSLSRTCTSVLAVEQDEKAAARAKANAEANGRANVTVENANAFDVLRRFDTSGRRFDTVV
EEHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHCCCCCEEEEEE
LDPPGLAKRREGLATALRAYHELNLRAFRCLKPDGLLVTCSCSGKLDRAAFEEMVLAAAA
ECCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHC
DAKRPVQILERRGAGLDHPVLAGLPETEYLKALYVRAL
CCCCHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SLMPPHAALPVARVTPKGARSLRHFNPWVYRTEIAAPPDVKGAGAVVLVVDSQGNPIGQ
CCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCHHH
ALYARRSPLALRLLTRKGPAEEPVDDAFFRRRLEAALARRAYLSGRDGLRLVHGEADQLP
HHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCC
GLFVDRYGKGLTLQTLSEGMDARKETLAKMLVELTGATHVMCRDDASGRDFEGLPREARL
CHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHE
LHGEGAARFTYHEGENRFEVDLQGDMKTGAFLDQVDNHLRAGELARGDALDLFSYHGGFA
EECCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEE
LSLSRTCTSVLAVEQDEKAAARAKANAEANGRANVTVENANAFDVLRRFDTSGRRFDTVV
EEHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHCCCCCEEEEEE
LDPPGLAKRREGLATALRAYHELNLRAFRCLKPDGLLVTCSCSGKLDRAAFEEMVLAAAA
ECCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHC
DAKRPVQILERRGAGLDHPVLAGLPETEYLKALYVRAL
CCCCHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA