Click here to switch to the map view.

The map label for this gene is hisH [H]

Identifier: 108762544

GI number: 108762544

Start: 5186045

End: 5186638

Strand: Reverse

Name: hisH [H]

Synonym: MXAN_4227

Alternate gene names: 108762544

Gene position: 5186638-5186045 (Counterclockwise)

Preceding gene: 108757852

Following gene: 108758023

Centisome position: 56.75

GC content: 70.88

Gene sequence:

>594_bases
ATGAGAGTCACCTTGTTCGACTACGGCGCGGGAAACCTCCACTCGCTGGCCAAGGCGCTGGCCACGGTGCCGGGCGCTGA
GGTGCGCGTCCAGGAGGACCCCTTGCGTGCGCTGGACACCGACGTGCTGGTGCTGCCGGGGGTGGGCGCATTCGGTGCGG
CGGTGGCGCGGCTCGAGCCGGGGCGCGAAGCCATGCGCGCGGCACTGGAGCAGGGCCTGCCGTGCCTGGGCATCTGCCTG
GGCATGCAGTTGCTCTTCGAAGGCAGTGACGAAGGCGAGGGCGCCGGCCTCGGTTACTTCGCGGGCCGGGTGAAACGGCT
GGCGGCGAAGCGAGTGCCCGAGATTGGCTGGAACCAGGTTGAGGACGAACGCACGCTGGCGGGTGCGCGGCTGGACACCG
TGTACTACGCGCACAGCTTCGTCTGCCGCGCCGCGGATGCTTCGGTGGTGACGGGGTGGACGACGCACGAGGCGGACCGC
TTCCCGGCGGCGGTGCGCAGGGGAAGGGTGGTGGGTGTGCAGTTCCACCCGGAGAAGTCCTCCACGGCGGGCGTGCGCTT
CGTGCAGGCCTTCCTGCGGGAGGTGGCGCCGTGA

Upstream 100 bases:

>100_bases
CGTTCCAGGCGCTGACGGGCGTGGGCGACGCGCTCCGAATCGGTGTGGGGCCCTGGCCCTTGATGGAGGCGGCGCTGGCG
GCGCTGCGGGAGGCGCTGCG

Downstream 100 bases:

>100_bases
TTGCGATTCCAGCCATCGATCTGCGCGAGGGCGCGTGTGTCCAACTCGTGGGCGGCTCGTACGCGGCGGAGAAGGTCCGG
GTGGAGGAACCGCTGGAGGC

Product: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MRVTLFDYGAGNLHSLAKALATVPGAEVRVQEDPLRALDTDVLVLPGVGAFGAAVARLEPGREAMRAALEQGLPCLGICL
GMQLLFEGSDEGEGAGLGYFAGRVKRLAAKRVPEIGWNQVEDERTLAGARLDTVYYAHSFVCRAADASVVTGWTTHEADR
FPAAVRRGRVVGVQFHPEKSSTAGVRFVQAFLREVAP

Sequences:

>Translated_197_residues
MRVTLFDYGAGNLHSLAKALATVPGAEVRVQEDPLRALDTDVLVLPGVGAFGAAVARLEPGREAMRAALEQGLPCLGICL
GMQLLFEGSDEGEGAGLGYFAGRVKRLAAKRVPEIGWNQVEDERTLAGARLDTVYYAHSFVCRAADASVVTGWTTHEADR
FPAAVRRGRVVGVQFHPEKSSTAGVRFVQAFLREVAP
>Mature_197_residues
MRVTLFDYGAGNLHSLAKALATVPGAEVRVQEDPLRALDTDVLVLPGVGAFGAAVARLEPGREAMRAALEQGLPCLGICL
GMQLLFEGSDEGEGAGLGYFAGRVKRLAAKRVPEIGWNQVEDERTLAGARLDTVYYAHSFVCRAADASVVTGWTTHEADR
FPAAVRRGRVVGVQFHPEKSSTAGVRFVQAFLREVAP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=206, Percent_Identity=36.4077669902913, Blast_Score=98, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319725, Length=212, Percent_Identity=35.377358490566, Blast_Score=108, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 21077; Mature: 21077

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVTLFDYGAGNLHSLAKALATVPGAEVRVQEDPLRALDTDVLVLPGVGAFGAAVARLEP
CEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCHHHHCCCEEEECCCCHHHHHHHHCCC
GREAMRAALEQGLPCLGICLGMQLLFEGSDEGEGAGLGYFAGRVKRLAAKRVPEIGWNQV
CHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
EDERTLAGARLDTVYYAHSFVCRAADASVVTGWTTHEADRFPAAVRRGRVVGVQFHPEKS
CHHHHHHCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHCCCEEEEEECCCCC
STAGVRFVQAFLREVAP
CHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVTLFDYGAGNLHSLAKALATVPGAEVRVQEDPLRALDTDVLVLPGVGAFGAAVARLEP
CEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCHHHHCCCEEEECCCCHHHHHHHHCCC
GREAMRAALEQGLPCLGICLGMQLLFEGSDEGEGAGLGYFAGRVKRLAAKRVPEIGWNQV
CHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
EDERTLAGARLDTVYYAHSFVCRAADASVVTGWTTHEADRFPAAVRRGRVVGVQFHPEKS
CHHHHHHCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHCCCEEEEEECCCCC
STAGVRFVQAFLREVAP
CHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA