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The map label for this gene is mrpE [H]

Identifier: 108761987

GI number: 108761987

Start: 4642150

End: 4642623

Strand: Direct

Name: mrpE [H]

Synonym: MXAN_3862

Alternate gene names: 108761987

Gene position: 4642150-4642623 (Clockwise)

Preceding gene: 108760979

Following gene: 108761232

Centisome position: 50.79

GC content: 66.24

Gene sequence:

>474_bases
ATGACCGCGTTCCTCTGGAACCTGATGCTGGCGCTCCTCTGGGCCGCGATGCTGGGGAACGTCACTTCGGAGAACCTCCT
CACGGGGTTCATCATCGGCTTCGTGCTGCTGGCCCTCATCGACACGCAGCATGTGCCCTCGCGCTATGGGACGCAGGCGT
GGAACGTGGTGCGGCTGGTGGCGGGGGTGGGCTGGGACGTGCTGGTCGCCAACGTGCGGGTGGCCTACGAGATTGCGACG
CCCAAGCTCCTCACCCGTCCCGCCATCTACCGCTACGACATGGAGGCACGCACGGACGCGGAGATCACGCTGCTGACGCT
CATCGTCACCTTCGCGCCGGGCACGCTGGCCCTGGAGGTGTCCGAGGACCGCAAGGCCATCTACGTCCACGTCATGTTCG
CCACCACCCGGGAGGCGTTCTGCCAGGAGCTCCGCGAGCGCGTGGAGATTCCACTGTTGAGGGCACTGCGATGA

Upstream 100 bases:

>100_bases
TGGGCCTGGGCGCCGAGCCACTGCTGGAGCTGGCGGACGCCGCCGCGGGCCAGTTGCTGGACCCGGAGGAGTACGTGCGG
GCGGTGCTGGGGGGACGCCC

Downstream 100 bases:

>100_bases
GCGACTGGCTGCAGACCATCACCCAGGGGGCCCTCGGGGCCATCTCCGTCTCGATGCTGTTCTCGCTCCACCGGCTGCTC
CGAGGTCCGTCGCTGACGGA

Product: Na+/H+ ion antiporter

Products: NA

Alternate protein names: Mrp complex subunit E; Multiple resistance and pH homeostasis protein E [H]

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MTAFLWNLMLALLWAAMLGNVTSENLLTGFIIGFVLLALIDTQHVPSRYGTQAWNVVRLVAGVGWDVLVANVRVAYEIAT
PKLLTRPAIYRYDMEARTDAEITLLTLIVTFAPGTLALEVSEDRKAIYVHVMFATTREAFCQELRERVEIPLLRALR

Sequences:

>Translated_157_residues
MTAFLWNLMLALLWAAMLGNVTSENLLTGFIIGFVLLALIDTQHVPSRYGTQAWNVVRLVAGVGWDVLVANVRVAYEIAT
PKLLTRPAIYRYDMEARTDAEITLLTLIVTFAPGTLALEVSEDRKAIYVHVMFATTREAFCQELRERVEIPLLRALR
>Mature_156_residues
TAFLWNLMLALLWAAMLGNVTSENLLTGFIIGFVLLALIDTQHVPSRYGTQAWNVVRLVAGVGWDVLVANVRVAYEIATP
KLLTRPAIYRYDMEARTDAEITLLTLIVTFAPGTLALEVSEDRKAIYVHVMFATTREAFCQELRERVEIPLLRALR

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1863

COG function: function code P; Multisubunit Na+/H+ antiporter, MnhE subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit E family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002758 [H]

Pfam domain/function: PF01899 MNHE [H]

EC number: NA

Molecular weight: Translated: 17680; Mature: 17549

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFLWNLMLALLWAAMLGNVTSENLLTGFIIGFVLLALIDTQHVPSRYGTQAWNVVRLV
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
AGVGWDVLVANVRVAYEIATPKLLTRPAIYRYDMEARTDAEITLLTLIVTFAPGTLALEV
HHCCHHHHHHHHEEEEEECCCHHHHCCHHEEECCCCCCCCHHHHHHHHHHHCCCEEEEEE
SEDRKAIYVHVMFATTREAFCQELRERVEIPLLRALR
CCCCEEEEEEEEEHHHHHHHHHHHHHHHCCHHHHHCC
>Mature Secondary Structure 
TAFLWNLMLALLWAAMLGNVTSENLLTGFIIGFVLLALIDTQHVPSRYGTQAWNVVRLV
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
AGVGWDVLVANVRVAYEIATPKLLTRPAIYRYDMEARTDAEITLLTLIVTFAPGTLALEV
HHCCHHHHHHHHEEEEEECCCHHHHCCHHEEECCCCCCCCHHHHHHHHHHHCCCEEEEEE
SEDRKAIYVHVMFATTREAFCQELRERVEIPLLRALR
CCCCEEEEEEEEEHHHHHHHHHHHHHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 10198001; 11356194 [H]