Click here to switch to the map view.

The map label for this gene is pgaC [H]

Identifier: 108761124

GI number: 108761124

Start: 6681841

End: 6682632

Strand: Direct

Name: pgaC [H]

Synonym: MXAN_5367

Alternate gene names: 108761124

Gene position: 6681841-6682632 (Clockwise)

Preceding gene: 108760051

Following gene: 108761925

Centisome position: 73.11

GC content: 65.53

Gene sequence:

>792_bases
GTGGCCCAATACCCAAGCATCAGCCTGTTCTTCCCCGCCTGGAACGAAGAGGACTACGTCGAGCGCGCCGTCAGCCGGGC
GCTGGAGGTCCTCCCCGCCCTGACGGACGACTTCGAAATCATCGTCGTCAACGACGCATCCACGGACCGCACCCGTGAGG
TCTGTGAGGCGCTGGCCCAGAAGGTCCCCCAACTGCGTGTCATCCACCACCCCGTGAATCTGAAGCTGGGTGGCGCCATG
CGCACCGGGCTGGCGGCCTCGACGAAGGACATCGTCGTGTACTCCGATGTAGACCTGCCCTGGGACATGCGCGAGCTGGA
GCGGGCGCTGCACCTGATGTCGTACCTGGAGGCGGACATGATTTGCGCCTTCCGGTTCGACCGCACGAGCGAGGGCGCCA
AGCGCATCATCTACTCGTTCGTCTACAACATGCTCATCCGGTCGCTGTTCGACATCCAGATCAAGGACGTCAACTTCAGC
TTCAAGGTGATGCACCGCCGCGTGCTGGAGGCCATGGAGCTGCACAGCCAGGGCTCGTTCATCGACGCGGAGCTCGTGGT
GAAGGCCATCCGCCAGGGCTTCCGCGTGTTCCAGATGGGCGTGGACTACTTCCCACGCACGCGCGGCGTGTCCACGCTGG
CCTCGCCCTCCGTCATCACGAAGATGGTGCGGGAGCTGGTGAAGCTCTATCCGAAGATGCGCGCGCCGCAGCCGCCAGTG
CACCCGGTGCGCTTGCCGCCTTCCGTGCAGCAGCTCCACGCGGTGCCGCCGGGCCGCACGGCGCGAGGCTGA

Upstream 100 bases:

>100_bases
TCATAGGCATGCGACGATATGCGACCCCTGCAATTGGATAAAAACCGGATTCTCTGCTAGCGAGCCGCCAACTTCGCAGG
TCCGCCCGCCGAGGTCCCTC

Downstream 100 bases:

>100_bases
GCCCCGCATGACGCGCCCCCTCACGCGCCTGGTGGTGAACGCGGACGACCTGGGGCTCCATGCCTCGCTGGACGCGGGCA
TCCTCCGCGCGCACCGCGAC

Product: group 2 family glycosyl transferase

Products: GDP; dolichyl D-mannosyl phosphate

Alternate protein names: PGA synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm PGA synthesis protein PgaC; N-acetylglucosaminyltransferase PgaC [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MAQYPSISLFFPAWNEEDYVERAVSRALEVLPALTDDFEIIVVNDASTDRTREVCEALAQKVPQLRVIHHPVNLKLGGAM
RTGLAASTKDIVVYSDVDLPWDMRELERALHLMSYLEADMICAFRFDRTSEGAKRIIYSFVYNMLIRSLFDIQIKDVNFS
FKVMHRRVLEAMELHSQGSFIDAELVVKAIRQGFRVFQMGVDYFPRTRGVSTLASPSVITKMVRELVKLYPKMRAPQPPV
HPVRLPPSVQQLHAVPPGRTARG

Sequences:

>Translated_263_residues
MAQYPSISLFFPAWNEEDYVERAVSRALEVLPALTDDFEIIVVNDASTDRTREVCEALAQKVPQLRVIHHPVNLKLGGAM
RTGLAASTKDIVVYSDVDLPWDMRELERALHLMSYLEADMICAFRFDRTSEGAKRIIYSFVYNMLIRSLFDIQIKDVNFS
FKVMHRRVLEAMELHSQGSFIDAELVVKAIRQGFRVFQMGVDYFPRTRGVSTLASPSVITKMVRELVKLYPKMRAPQPPV
HPVRLPPSVQQLHAVPPGRTARG
>Mature_262_residues
AQYPSISLFFPAWNEEDYVERAVSRALEVLPALTDDFEIIVVNDASTDRTREVCEALAQKVPQLRVIHHPVNLKLGGAMR
TGLAASTKDIVVYSDVDLPWDMRELERALHLMSYLEADMICAFRFDRTSEGAKRIIYSFVYNMLIRSLFDIQIKDVNFSF
KVMHRRVLEAMELHSQGSFIDAELVVKAIRQGFRVFQMGVDYFPRTRGVSTLASPSVITKMVRELVKLYPKMRAPQPPVH
PVRLPPSVQQLHAVPPGRTARG

Specific function: Probable N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N- acetylglucosamine to produce the linear homopolymer poly-beta-1,6- N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide [H]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=94, Percent_Identity=40.4255319148936, Blast_Score=71, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI71999402, Length=227, Percent_Identity=22.9074889867841, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24585265, Length=233, Percent_Identity=24.0343347639485, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: 2.4.1.83

Molecular weight: Translated: 29960; Mature: 29829

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQYPSISLFFPAWNEEDYVERAVSRALEVLPALTDDFEIIVVNDASTDRTREVCEALAQ
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHH
KVPQLRVIHHPVNLKLGGAMRTGLAASTKDIVVYSDVDLPWDMRELERALHLMSYLEADM
HCCCEEEEECCCCEEECCHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
ICAFRFDRTSEGAKRIIYSFVYNMLIRSLFDIQIKDVNFSFKVMHRRVLEAMELHSQGSF
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHCCCCE
IDAELVVKAIRQGFRVFQMGVDYFPRTRGVSTLASPSVITKMVRELVKLYPKMRAPQPPV
EHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCC
HPVRLPPSVQQLHAVPPGRTARG
CCCCCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AQYPSISLFFPAWNEEDYVERAVSRALEVLPALTDDFEIIVVNDASTDRTREVCEALAQ
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHH
KVPQLRVIHHPVNLKLGGAMRTGLAASTKDIVVYSDVDLPWDMRELERALHLMSYLEADM
HCCCEEEEECCCCEEECCHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
ICAFRFDRTSEGAKRIIYSFVYNMLIRSLFDIQIKDVNFSFKVMHRRVLEAMELHSQGSF
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHCCCCE
IDAELVVKAIRQGFRVFQMGVDYFPRTRGVSTLASPSVITKMVRELVKLYPKMRAPQPPV
EHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCC
HPVRLPPSVQQLHAVPPGRTARG
CCCCCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDPmannose; dolichyl phosphate

Specific reaction: GDP-mannose + dolichyl phosphate = GDP + dolichyl D-mannosyl phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]