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The map label for this gene is bkdB [H]

Identifier: 108761010

GI number: 108761010

Start: 5176806

End: 5178056

Strand: Reverse

Name: bkdB [H]

Synonym: MXAN_4217

Alternate gene names: 108761010

Gene position: 5178056-5176806 (Counterclockwise)

Preceding gene: 108762001

Following gene: 108758514

Centisome position: 56.65

GC content: 68.59

Gene sequence:

>1251_bases
ATGGCGATCTTCGAATTCAAGCTCCCCGACCTTGGTGAAGGCGTGATGGAGGGTGAGCTGGTGAAGTGGCACGTGAAGGC
AGGGGATTCCGTCAAGGAAGACCAGGTGCTCGCCGAGGTGATGACGGACAAGGCCACCGTCACCGTTCCCGCTCCCAAGG
CGGGCCGTGTCGTGAAGACGCATGGCAATGAAGGCGACATGGCGAAGGTGCACCAGCTCCTCGTCACCCTGGAGGTGGAG
GGCGCTGCGCCGGCGCAGGCCGGTGGTCATTCCGAGGCCAGCGCGCCAGCGGCGGCGCCCGTGGCGGGTGGCCATGTCGG
TGGTGCGCCTGCCTCGGCGTCCAAGGTGCTGGCCACGCCGGTGACGCGGCGGATGGCGCGCGAGCACGGGCTGGACCTGG
CGTCGATTGCCGGCACGGGGCCACAGGGCCGCGTGACGAAGGCGGATGTGGTGGCGGCGCTGGAGGGTGGCGAGAAGAAC
GTCGTCGCGGCTCCGGCCGAGCAGAAGGCGCGTCCCGCGGCTCCTGCCGTCAGCTCGGGCGCGGCGGACGAGCGCGTTCC
GCTTCGGGGCCTGCGCAAGAAGATCGCGGAGAAGATGGTGCGGTCGAAGTTCACGGCGCCGCACTTCGCCTTTGTCGAGG
AAGTGGACGCCACGGAGCTGGTGGCCCTGCGCGCGCGGCTGAACGCGCAGTTGGCGGCGGCCGGTGAGAACATCAAGCTC
AACTACCTGCCGTTCATCATCAAGGCGACGGTGGCGGCGCTGAAGAAGTTCCCGCACCTGAACGCGAACTTCGACGAGGC
GTCGCAGGAGCTGGTGGTCCGCGGCGAGTTCAACATCGGCATGGCGGCGGCGACGCCGGACGGCCTCACCGTGGCGGTGG
TGAAGAGCGCGGACCGGCTGACGCTGGCCGAGCTGGCGCGTGAGACGGCCCGCCTGGGCGCCGCCGCGCGTGACCGCAAG
CTGAAGATGGAGGAGCTGACGGGCGGCACCTTCACCATCAGCTCGCTGGGGCAGAGCGGCGGCCTGTTCGCCACGCCCAT
CATCAACCACCCTGAAGTGGGCATCCTGGGCGTGCACCGCCTGAAGAAGCGCCCGGCGGTGGTGGGGGACCAGGTCGTCG
TGCGCGACATGATGAACCTGTCGCTGTCGTGCGACCACCGCGTCATCGACGGCTCGGTGGCGGCGGATTTCACGTACGAA
ATCATCAAGTACCTGGAGAAGCCGGACCTGCTGTTCCTGGCCATGGCGTGA

Upstream 100 bases:

>100_bases
GGGCCTGATGGAGCGCGAGCGGCTCGAGCGGCTCGGCTGAGCTTTGACGCTCCGATTCATGGCTTGACCCAACACCCCTG
CTTCCCGGAAAGACGACCCC

Downstream 100 bases:

>100_bases
GGTGAGCGCCGTGGCAGGTCCACGCCCCTGACGTGAGGGGCGTGGTGGCCCTGGAGCCGGGCCGGGAAGCGCCTGATGCT
GGGGCCCGCGTTCGAGGGCC

Product: alpha keto acid dehydrogenase complex, E2 component, dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MAIFEFKLPDLGEGVMEGELVKWHVKAGDSVKEDQVLAEVMTDKATVTVPAPKAGRVVKTHGNEGDMAKVHQLLVTLEVE
GAAPAQAGGHSEASAPAAAPVAGGHVGGAPASASKVLATPVTRRMAREHGLDLASIAGTGPQGRVTKADVVAALEGGEKN
VVAAPAEQKARPAAPAVSSGAADERVPLRGLRKKIAEKMVRSKFTAPHFAFVEEVDATELVALRARLNAQLAAAGENIKL
NYLPFIIKATVAALKKFPHLNANFDEASQELVVRGEFNIGMAAATPDGLTVAVVKSADRLTLAELARETARLGAAARDRK
LKMEELTGGTFTISSLGQSGGLFATPIINHPEVGILGVHRLKKRPAVVGDQVVVRDMMNLSLSCDHRVIDGSVAADFTYE
IIKYLEKPDLLFLAMA

Sequences:

>Translated_416_residues
MAIFEFKLPDLGEGVMEGELVKWHVKAGDSVKEDQVLAEVMTDKATVTVPAPKAGRVVKTHGNEGDMAKVHQLLVTLEVE
GAAPAQAGGHSEASAPAAAPVAGGHVGGAPASASKVLATPVTRRMAREHGLDLASIAGTGPQGRVTKADVVAALEGGEKN
VVAAPAEQKARPAAPAVSSGAADERVPLRGLRKKIAEKMVRSKFTAPHFAFVEEVDATELVALRARLNAQLAAAGENIKL
NYLPFIIKATVAALKKFPHLNANFDEASQELVVRGEFNIGMAAATPDGLTVAVVKSADRLTLAELARETARLGAAARDRK
LKMEELTGGTFTISSLGQSGGLFATPIINHPEVGILGVHRLKKRPAVVGDQVVVRDMMNLSLSCDHRVIDGSVAADFTYE
IIKYLEKPDLLFLAMA
>Mature_415_residues
AIFEFKLPDLGEGVMEGELVKWHVKAGDSVKEDQVLAEVMTDKATVTVPAPKAGRVVKTHGNEGDMAKVHQLLVTLEVEG
AAPAQAGGHSEASAPAAAPVAGGHVGGAPASASKVLATPVTRRMAREHGLDLASIAGTGPQGRVTKADVVAALEGGEKNV
VAAPAEQKARPAAPAVSSGAADERVPLRGLRKKIAEKMVRSKFTAPHFAFVEEVDATELVALRARLNAQLAAAGENIKLN
YLPFIIKATVAALKKFPHLNANFDEASQELVVRGEFNIGMAAATPDGLTVAVVKSADRLTLAELARETARLGAAARDRKL
KMEELTGGTFTISSLGQSGGLFATPIINHPEVGILGVHRLKKRPAVVGDQVVVRDMMNLSLSCDHRVIDGSVAADFTYEI
IKYLEKPDLLFLAMA

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=429, Percent_Identity=34.032634032634, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI31711992, Length=434, Percent_Identity=31.3364055299539, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI19923748, Length=244, Percent_Identity=36.0655737704918, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=28.3813747228381, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI203098816, Length=450, Percent_Identity=28, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI260898739, Length=164, Percent_Identity=31.7073170731707, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786946, Length=418, Percent_Identity=36.1244019138756, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1786305, Length=409, Percent_Identity=38.8753056234719, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17537937, Length=422, Percent_Identity=34.1232227488152, Blast_Score=248, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI25146366, Length=420, Percent_Identity=31.6666666666667, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17560088, Length=443, Percent_Identity=31.3769751693002, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17538894, Length=308, Percent_Identity=30.1948051948052, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=420, Percent_Identity=31.6666666666667, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6324258, Length=451, Percent_Identity=29.2682926829268, Blast_Score=144, Evalue=3e-35,
Organism=Drosophila melanogaster, GI18859875, Length=437, Percent_Identity=35.4691075514874, Blast_Score=222, Evalue=5e-58,
Organism=Drosophila melanogaster, GI20129315, Length=445, Percent_Identity=28.0898876404494, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24582497, Length=435, Percent_Identity=27.816091954023, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24645909, Length=230, Percent_Identity=33.9130434782609, Blast_Score=128, Evalue=7e-30,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 43704; Mature: 43573

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIFEFKLPDLGEGVMEGELVKWHVKAGDSVKEDQVLAEVMTDKATVTVPAPKAGRVVKT
CEEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCEEEE
HGNEGDMAKVHQLLVTLEVEGAAPAQAGGHSEASAPAAAPVAGGHVGGAPASASKVLATP
CCCCCCHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
VTRRMAREHGLDLASIAGTGPQGRVTKADVVAALEGGEKNVVAAPAEQKARPAAPAVSSG
HHHHHHHHHCCCEEECCCCCCCCCCCHHHEEEEECCCCCCEEECCCHHCCCCCCCCCCCC
AADERVPLRGLRKKIAEKMVRSKFTAPHFAFVEEVDATELVALRARLNAQLAAAGENIKL
CCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHCCEEEECCCCEEE
NYLPFIIKATVAALKKFPHLNANFDEASQELVVRGEFNIGMAAATPDGLTVAVVKSADRL
EHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCEEEEEECCCCCH
TLAELARETARLGAAARDRKLKMEELTGGTFTISSLGQSGGLFATPIINHPEVGILGVHR
HHHHHHHHHHHHCCHHHCCCCCHHHCCCCEEEEECCCCCCCEEECCCCCCCCCCEEEHHH
LKKRPAVVGDQVVVRDMMNLSLSCDHRVIDGSVAADFTYEIIKYLEKPDLLFLAMA
HHCCCCCCCCHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEEEC
>Mature Secondary Structure 
AIFEFKLPDLGEGVMEGELVKWHVKAGDSVKEDQVLAEVMTDKATVTVPAPKAGRVVKT
EEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEECCCCCCCEEEE
HGNEGDMAKVHQLLVTLEVEGAAPAQAGGHSEASAPAAAPVAGGHVGGAPASASKVLATP
CCCCCCHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
VTRRMAREHGLDLASIAGTGPQGRVTKADVVAALEGGEKNVVAAPAEQKARPAAPAVSSG
HHHHHHHHHCCCEEECCCCCCCCCCCHHHEEEEECCCCCCEEECCCHHCCCCCCCCCCCC
AADERVPLRGLRKKIAEKMVRSKFTAPHFAFVEEVDATELVALRARLNAQLAAAGENIKL
CCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHCCEEEECCCCEEE
NYLPFIIKATVAALKKFPHLNANFDEASQELVVRGEFNIGMAAATPDGLTVAVVKSADRL
EHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCEEEEEECCCCCH
TLAELARETARLGAAARDRKLKMEELTGGTFTISSLGQSGGLFATPIINHPEVGILGVHR
HHHHHHHHHHHHCCHHHCCCCCHHHCCCCEEEEECCCCCCCEEECCCCCCCCCCEEEHHH
LKKRPAVVGDQVVVRDMMNLSLSCDHRVIDGSVAADFTYEIIKYLEKPDLLFLAMA
HHCCCCCCCCHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 3046941 [H]