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The map label for this gene is lipA [H]

Identifier: 108762001

GI number: 108762001

Start: 5178117

End: 5179046

Strand: Reverse

Name: lipA [H]

Synonym: MXAN_4218

Alternate gene names: 108762001

Gene position: 5179046-5178117 (Counterclockwise)

Preceding gene: 108758911

Following gene: 108761010

Centisome position: 56.66

GC content: 68.28

Gene sequence:

>930_bases
ATGGCGACTCCCGACCGGTTCCCTCTGCCCCAGGTGACTGAGACCACCCGCAAGCCGGAGTGGCTGAAGGTGCGGCTCCC
GCACGGAGAAGGTTACGAGCGGGTCAAAGCCATCGTGAAGCGCACCAAGCTGGCCACGGTGTGCGAAGAGGCCCGCTGCC
CGAACATCGCCGAGTGCTGGGGCGGTGGCACCGCCACGGTGATGCTGATGGGCGAGGTGTGCACGCGCGCGTGCCGCTTC
TGCCACGTGAAGGTCGGCGCGCCTCCTCCGCTGGATCCGATGGAGCCCATCCATCTGGCCCAGGCGGTGAAGGAGATGGA
CCTGGAGTACATCGTCGTCACGTCCGTGAACCGGGATGACCGGCCGGACGGCGGCGCCAGCCACTTCGCTTCCGCCATCC
GCGAGCTGCGCCGGGAGAGCCCGCGCACCATCGTCGAAGTGCTCATCCCGGACTTCAAGGGCGTGGAGAAGGACCTGACC
ACCGTGGCGGAGGCCAAGCCCCACGTGGTGGCGCACAACGTGGAGACGGTGGAGCGCCTCACGCCGACGGTCCGTGACCG
GCGCGCGAAGTACCACCAGTCCCTGCGCGTGCTGGAGTACCTCAAGAACCGCCCCGAGGGCCTGTACACCAAGACGTCCG
TCATGGTGGGCCTGGGGGAGACGGACGCGGAGCTGGAGCAGACGTTCAAGGACCTGCGCGACGTGGGCGTGGACGTGCTG
ACGCTGGGCCAGTACCTGCAGCCGTCGCAGTACCACCTGCGCGTGGAGCGCTTCGTGACGCCCGCGCAGTTCGAGGCGTA
CAAGACGCTGGCCGAGTCCTACGGCTTCCTCTACGTGGCCTCCGGTCCGCTGGTGCGGTCCAGCTACCGGGCCGCTGAGT
TCTTCATGAAGGGCCTGATGGAGCGCGAGCGGCTCGAGCGGCTCGGCTGA

Upstream 100 bases:

>100_bases
GAGAGGCGACAACTGGCGGACGACTGGTGGATGCTGACGCCTCGCGTCAGTGGCTTCCGCTTGAACGTCCTCCGTCCAAC
CCATAGAAGGCCCGCGATCC

Downstream 100 bases:

>100_bases
GCTTTGACGCTCCGATTCATGGCTTGACCCAACACCCCTGCTTCCCGGAAAGACGACCCCATGGCGATCTTCGAATTCAA
GCTCCCCGACCTTGGTGAAG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MATPDRFPLPQVTETTRKPEWLKVRLPHGEGYERVKAIVKRTKLATVCEEARCPNIAECWGGGTATVMLMGEVCTRACRF
CHVKVGAPPPLDPMEPIHLAQAVKEMDLEYIVVTSVNRDDRPDGGASHFASAIRELRRESPRTIVEVLIPDFKGVEKDLT
TVAEAKPHVVAHNVETVERLTPTVRDRRAKYHQSLRVLEYLKNRPEGLYTKTSVMVGLGETDAELEQTFKDLRDVGVDVL
TLGQYLQPSQYHLRVERFVTPAQFEAYKTLAESYGFLYVASGPLVRSSYRAAEFFMKGLMERERLERLG

Sequences:

>Translated_309_residues
MATPDRFPLPQVTETTRKPEWLKVRLPHGEGYERVKAIVKRTKLATVCEEARCPNIAECWGGGTATVMLMGEVCTRACRF
CHVKVGAPPPLDPMEPIHLAQAVKEMDLEYIVVTSVNRDDRPDGGASHFASAIRELRRESPRTIVEVLIPDFKGVEKDLT
TVAEAKPHVVAHNVETVERLTPTVRDRRAKYHQSLRVLEYLKNRPEGLYTKTSVMVGLGETDAELEQTFKDLRDVGVDVL
TLGQYLQPSQYHLRVERFVTPAQFEAYKTLAESYGFLYVASGPLVRSSYRAAEFFMKGLMERERLERLG
>Mature_308_residues
ATPDRFPLPQVTETTRKPEWLKVRLPHGEGYERVKAIVKRTKLATVCEEARCPNIAECWGGGTATVMLMGEVCTRACRFC
HVKVGAPPPLDPMEPIHLAQAVKEMDLEYIVVTSVNRDDRPDGGASHFASAIRELRRESPRTIVEVLIPDFKGVEKDLTT
VAEAKPHVVAHNVETVERLTPTVRDRRAKYHQSLRVLEYLKNRPEGLYTKTSVMVGLGETDAELEQTFKDLRDVGVDVLT
LGQYLQPSQYHLRVERFVTPAQFEAYKTLAESYGFLYVASGPLVRSSYRAAEFFMKGLMERERLERLG

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=304, Percent_Identity=50.6578947368421, Blast_Score=320, Evalue=1e-87,
Organism=Homo sapiens, GI37577164, Length=252, Percent_Identity=51.5873015873016, Blast_Score=265, Evalue=4e-71,
Organism=Escherichia coli, GI1786846, Length=285, Percent_Identity=47.0175438596491, Blast_Score=264, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI32564533, Length=293, Percent_Identity=52.5597269624573, Blast_Score=305, Evalue=3e-83,
Organism=Saccharomyces cerevisiae, GI6324770, Length=303, Percent_Identity=51.1551155115512, Blast_Score=320, Evalue=2e-88,
Organism=Drosophila melanogaster, GI221513272, Length=294, Percent_Identity=53.7414965986395, Blast_Score=337, Evalue=7e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 34960; Mature: 34828

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPDRFPLPQVTETTRKPEWLKVRLPHGEGYERVKAIVKRTKLATVCEEARCPNIAECW
CCCCCCCCCCCHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
GGGTATVMLMGEVCTRACRFCHVKVGAPPPLDPMEPIHLAQAVKEMDLEYIVVTSVNRDD
CCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCC
RPDGGASHFASAIRELRRESPRTIVEVLIPDFKGVEKDLTTVAEAKPHVVAHNVETVERL
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCHHHHHHH
TPTVRDRRAKYHQSLRVLEYLKNRPEGLYTKTSVMVGLGETDAELEQTFKDLRDVGVDVL
CHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHEEEEECCCCHHHHHHHHHHHHHCCCHHH
TLGQYLQPSQYHLRVERFVTPAQFEAYKTLAESYGFLYVASGPLVRSSYRAAEFFMKGLM
HHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHH
ERERLERLG
HHHHHHHCC
>Mature Secondary Structure 
ATPDRFPLPQVTETTRKPEWLKVRLPHGEGYERVKAIVKRTKLATVCEEARCPNIAECW
CCCCCCCCCCHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
GGGTATVMLMGEVCTRACRFCHVKVGAPPPLDPMEPIHLAQAVKEMDLEYIVVTSVNRDD
CCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCC
RPDGGASHFASAIRELRRESPRTIVEVLIPDFKGVEKDLTTVAEAKPHVVAHNVETVERL
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCHHHHHHH
TPTVRDRRAKYHQSLRVLEYLKNRPEGLYTKTSVMVGLGETDAELEQTFKDLRDVGVDVL
CHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHEEEEECCCCHHHHHHHHHHHHHCCCHHH
TLGQYLQPSQYHLRVERFVTPAQFEAYKTLAESYGFLYVASGPLVRSSYRAAEFFMKGLM
HHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHH
ERERLERLG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA