Click here to switch to the map view.

The map label for this gene is sigB [H]

Identifier: 108760621

GI number: 108760621

Start: 3903316

End: 3904056

Strand: Reverse

Name: sigB [H]

Synonym: MXAN_3357

Alternate gene names: 108760621

Gene position: 3904056-3903316 (Counterclockwise)

Preceding gene: 108759976

Following gene: 108762092

Centisome position: 42.72

GC content: 65.99

Gene sequence:

>741_bases
ATGGTGACCGCCAACCTGCGCTTCGTGGTGAAGGTCGCCTATGAGTACCGCTCATACGGCATCAAGATGTCGGACCTCAT
CCAGGAGGGGAACATCGGCCTGATGAAGGCCGTGCAGAAGTTCGATCCGGACAAGGGCATCCGCCTCATCTCCTACGCGG
TGTGGTGGATTCGCGCGTACATCCAGAACTACATCCTCAAGAGCTGGTCGCTCGTGAAGCTGGGGACCACCCAGGCGCAG
CGGAAGCTGTTCTTCAGTCTGGCCCGCACGCGCCGCGAGCTGGAGAAGTTTGGCAGCGGTGACGCCGCGGTGAACGTGGA
TGACATCGCCCGCCGGCTCCACGTGAAGCCGGGCGAGGTGCGGGAGATGGAGCAGCGCATGGGGGGCCGGGACCTGTCGC
TGGACGCGCCCATGGGCGAGGACGGCGGCAACAGCCACGTGGACTTCGTGGTGAGCGCCGCGGCCCCGCAGGACGACGAG
TTCGCGGACAAGGAGGAGGCGGGCCTCATCAACGCCCGCGTCCGTACCGCGCTGATGCGCCTGGATCCGCGTGAGCGCTT
CATCATCGAGCAACGCGTCATGAACGAGCGCCCCATGACGCTCAAGGAACTGGGTGAGCACTTCGGCTTCTCGCGCGAGC
GCGCCCGCCAGTTGGAGATTCGCGCCAAGGACAAGCTCAAGTCGGAGCTGGCCGCGCTCATGGCCGAGGTGGACCCCGAG
GCCGTCGCCGCCCAGCAGTGA

Upstream 100 bases:

>100_bases
GTACCTCTCGGAGATCAACCACTACTCCCTCCTCAAGGTGGAGGAGGAGCAGGAGCTCGCGCGCCGCTTCCTCAAGGGCG
ACCTGGCGGCCGGGCACCGG

Downstream 100 bases:

>100_bases
GCCGGGACTGACCCGGCCCGGCGACCCGGCATTCCGGGGCAAGCCCGCATCAAGGCGCCTCCGCCCTACTGGCAGGGGCG
CTTGTCATTTGAGGGCGTTG

Product: RNA polymerase sigma-B factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MVTANLRFVVKVAYEYRSYGIKMSDLIQEGNIGLMKAVQKFDPDKGIRLISYAVWWIRAYIQNYILKSWSLVKLGTTQAQ
RKLFFSLARTRRELEKFGSGDAAVNVDDIARRLHVKPGEVREMEQRMGGRDLSLDAPMGEDGGNSHVDFVVSAAAPQDDE
FADKEEAGLINARVRTALMRLDPRERFIIEQRVMNERPMTLKELGEHFGFSRERARQLEIRAKDKLKSELAALMAEVDPE
AVAAQQ

Sequences:

>Translated_246_residues
MVTANLRFVVKVAYEYRSYGIKMSDLIQEGNIGLMKAVQKFDPDKGIRLISYAVWWIRAYIQNYILKSWSLVKLGTTQAQ
RKLFFSLARTRRELEKFGSGDAAVNVDDIARRLHVKPGEVREMEQRMGGRDLSLDAPMGEDGGNSHVDFVVSAAAPQDDE
FADKEEAGLINARVRTALMRLDPRERFIIEQRVMNERPMTLKELGEHFGFSRERARQLEIRAKDKLKSELAALMAEVDPE
AVAAQQ
>Mature_246_residues
MVTANLRFVVKVAYEYRSYGIKMSDLIQEGNIGLMKAVQKFDPDKGIRLISYAVWWIRAYIQNYILKSWSLVKLGTTQAQ
RKLFFSLARTRRELEKFGSGDAAVNVDDIARRLHVKPGEVREMEQRMGGRDLSLDAPMGEDGGNSHVDFVVSAAAPQDDE
FADKEEAGLINARVRTALMRLDPRERFIIEQRVMNERPMTLKELGEHFGFSRERARQLEIRAKDKLKSELAALMAEVDPE
AVAAQQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=247, Percent_Identity=40.4858299595142, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1789098, Length=231, Percent_Identity=31.6017316017316, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789448, Length=233, Percent_Identity=31.3304721030043, Blast_Score=101, Evalue=4e-23,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 28021; Mature: 28021

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTANLRFVVKVAYEYRSYGIKMSDLIQEGNIGLMKAVQKFDPDKGIRLISYAVWWIRAY
CCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
IQNYILKSWSLVKLGTTQAQRKLFFSLARTRRELEKFGSGDAAVNVDDIARRLHVKPGEV
HHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCHH
REMEQRMGGRDLSLDAPMGEDGGNSHVDFVVSAAAPQDDEFADKEEAGLINARVRTALMR
HHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHCCHHHHHHHHHHHH
LDPRERFIIEQRVMNERPMTLKELGEHFGFSRERARQLEIRAKDKLKSELAALMAEVDPE
CCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AVAAQQ
HHCCCC
>Mature Secondary Structure
MVTANLRFVVKVAYEYRSYGIKMSDLIQEGNIGLMKAVQKFDPDKGIRLISYAVWWIRAY
CCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
IQNYILKSWSLVKLGTTQAQRKLFFSLARTRRELEKFGSGDAAVNVDDIARRLHVKPGEV
HHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCHH
REMEQRMGGRDLSLDAPMGEDGGNSHVDFVVSAAAPQDDEFADKEEAGLINARVRTALMR
HHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHCCHHHHHHHHHHHH
LDPRERFIIEQRVMNERPMTLKELGEHFGFSRERARQLEIRAKDKLKSELAALMAEVDPE
CCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AVAAQQ
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1475207 [H]