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The map label for this gene is aslB [C]

Identifier: 108760123

GI number: 108760123

Start: 6459304

End: 6460809

Strand: Reverse

Name: aslB [C]

Synonym: MXAN_5172

Alternate gene names: 108760123

Gene position: 6460809-6459304 (Counterclockwise)

Preceding gene: 108757596

Following gene: 108762743

Centisome position: 70.69

GC content: 64.28

Gene sequence:

>1506_bases
ATGAGGGTCCCAGGGTTGCGTTATCGCGACGAGTGGCTGCCCAACTTCTTCCCCGCGAAGCAGGTGGGCCCGGACCACTT
CGTGCGGCTGTCCCGGACGGGCGCCATGCAACTGCTGTCCGGCGCGGAGAATGCCCAGCTCGATGAAATCTCCATGGATG
AGGCCCTGTTCGAGCGGCTCGAGCAGACCAGCCACCTCATCACCGCGCAGAACTCGTCGCGCGTGCTGGAGGACCTCAAG
CGCTGGCATGACCGGACCTACGCGGGGCCGGGGCTCCATATCGTCGTGCTGTCCAAGCGGTGCAACCTCAACTGCACCTA
CTGCCACATGAACCCGGAGAACATCTCCGCGGACTCCAAGCGCTTCGACATGCAGCCCGACGTCGCGGATGCCATCATCC
GCTTCGCGCTCGGTTCTCCGGCCCCCTTCCTGGGTTTCGAGTTCCAGGGCGGCGAGCCCTTCTTGAACTTCGAGGGCATG
AAGTACTTCGTCGAGGAGGCCCGCAGGCAGAACGAAGCCGTGGGCAAGCGGCTCCGCTTCTCCGTGGTGAGCAACCTCAT
GGTCGCCACCGACGAGCACATCGCGTACTGCAAGGAGAACCGCATCAACGTGTCGTATTCGTTGAACGGTCCCGAGGACA
TTCACGACTCATTCCGAGTCACTCGGAGCGGGGCGGGCAGCTTCCGCAAGGTCATGGAGCGGCTGAGCCACTTCCGCGAC
CGATTCCCCGGCGTGCTCAGCGCGTCGCCCCTGTGCGTGGTGGGGGCCTCCAACGCCAAGGAGCTGAAGCGCACCATCGA
CTTCTATCATGCGGAGGGCTTCAAGGACCTCGCCATCCTCAAGCTCAAGGCCCTGGGCAACGCCCGCAAGGAGAAGCTGC
CCTTCGACATCCAGGACTTCCTCCACTACTACCTGGACGCGCTCGATTACATCTACGAGAAGAACCGCCAGGAGAACGAG
AACTACTCCGAGCGCGTGGTCCGGGTCGTCCTGGCCAAGATTCTCCAGTCGACGGATACGGGCTTCGTGGACTGGCGCAA
CCCGGTCGGGGACTTCGGTTCGGCCATCACCTATGACCATGACGGCGAAATCCTGCCGTCGGACGAAGCCCGTTCGATGC
GCTCCGAGTTCGGCTTGGGCAACGTGAAGGGGACGACCTATGACGCGCTCATCCGCAACAAGGCCACGTTCCGGTCCATG
AACCTGTCGCTGCGGGATCGTGACCCGCAGTGCCGCGAGTGCGCCTTCAATCCCTACTGCGGCGTGCTGCCGGTGCTCGA
CTATGCCCGGACCGGGGACGCGACGCCGCGCCCCCATGCGTCCGAGGAATGCCTGCAGACGTTGGCGGTCGTGGAGTGGG
TCCTGCGCAAGCTCGTGGAGGACCCCATCCCCCTGTTGCGGATGATGCCTGGAATGGACGCCGCCCTGGTGGAGATGGCC
AACGGTCTGCCGACAGAGCCTTCCTCGCAGGCCATGCCGCCGATGCTGAGCGTGCAGAGCGGCTGA

Upstream 100 bases:

>100_bases
CCGCCAGCGGTCCCGTGGTGAATGACGCCGAGACATACATGGGCTGGCGCGAGGCGGCAGTGCTCGCGGCCCGCTATACC
CAGCACGCCGGAGGTGCCGC

Downstream 100 bases:

>100_bases
GGCCAGCCGGGGCTGGCGCCCTCGCCGCTGCTCAGGCGGGGCGCAGCCGCCCGGCGGCGAGGTCCGTGAGCGCGCCGAGG
ACTTCTTCCTCGGTGATGTC

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: AnSME; Cys-type sulfatase-activating enzyme [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MRVPGLRYRDEWLPNFFPAKQVGPDHFVRLSRTGAMQLLSGAENAQLDEISMDEALFERLEQTSHLITAQNSSRVLEDLK
RWHDRTYAGPGLHIVVLSKRCNLNCTYCHMNPENISADSKRFDMQPDVADAIIRFALGSPAPFLGFEFQGGEPFLNFEGM
KYFVEEARRQNEAVGKRLRFSVVSNLMVATDEHIAYCKENRINVSYSLNGPEDIHDSFRVTRSGAGSFRKVMERLSHFRD
RFPGVLSASPLCVVGASNAKELKRTIDFYHAEGFKDLAILKLKALGNARKEKLPFDIQDFLHYYLDALDYIYEKNRQENE
NYSERVVRVVLAKILQSTDTGFVDWRNPVGDFGSAITYDHDGEILPSDEARSMRSEFGLGNVKGTTYDALIRNKATFRSM
NLSLRDRDPQCRECAFNPYCGVLPVLDYARTGDATPRPHASEECLQTLAVVEWVLRKLVEDPIPLLRMMPGMDAALVEMA
NGLPTEPSSQAMPPMLSVQSG

Sequences:

>Translated_501_residues
MRVPGLRYRDEWLPNFFPAKQVGPDHFVRLSRTGAMQLLSGAENAQLDEISMDEALFERLEQTSHLITAQNSSRVLEDLK
RWHDRTYAGPGLHIVVLSKRCNLNCTYCHMNPENISADSKRFDMQPDVADAIIRFALGSPAPFLGFEFQGGEPFLNFEGM
KYFVEEARRQNEAVGKRLRFSVVSNLMVATDEHIAYCKENRINVSYSLNGPEDIHDSFRVTRSGAGSFRKVMERLSHFRD
RFPGVLSASPLCVVGASNAKELKRTIDFYHAEGFKDLAILKLKALGNARKEKLPFDIQDFLHYYLDALDYIYEKNRQENE
NYSERVVRVVLAKILQSTDTGFVDWRNPVGDFGSAITYDHDGEILPSDEARSMRSEFGLGNVKGTTYDALIRNKATFRSM
NLSLRDRDPQCRECAFNPYCGVLPVLDYARTGDATPRPHASEECLQTLAVVEWVLRKLVEDPIPLLRMMPGMDAALVEMA
NGLPTEPSSQAMPPMLSVQSG
>Mature_501_residues
MRVPGLRYRDEWLPNFFPAKQVGPDHFVRLSRTGAMQLLSGAENAQLDEISMDEALFERLEQTSHLITAQNSSRVLEDLK
RWHDRTYAGPGLHIVVLSKRCNLNCTYCHMNPENISADSKRFDMQPDVADAIIRFALGSPAPFLGFEFQGGEPFLNFEGM
KYFVEEARRQNEAVGKRLRFSVVSNLMVATDEHIAYCKENRINVSYSLNGPEDIHDSFRVTRSGAGSFRKVMERLSHFRD
RFPGVLSASPLCVVGASNAKELKRTIDFYHAEGFKDLAILKLKALGNARKEKLPFDIQDFLHYYLDALDYIYEKNRQENE
NYSERVVRVVLAKILQSTDTGFVDWRNPVGDFGSAITYDHDGEILPSDEARSMRSEFGLGNVKGTTYDALIRNKATFRSM
NLSLRDRDPQCRECAFNPYCGVLPVLDYARTGDATPRPHASEECLQTLAVVEWVLRKLVEDPIPLLRMMPGMDAALVEMA
NGLPTEPSSQAMPPMLSVQSG

Specific function: Involved in 'Cys-type' sulfatase maturation under anaerobic conditions. Catalyzes the post-translational modification of cysteine ('Cys-51' in the arylsulfatase CPF_0221) into 3-oxoalanine (also known as C(alpha)-formylglycine (FGly)), by a free radical c

COG id: COG0641

COG function: function code R; Arylsulfatase regulator (Fe-S oxidoreductase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994973, Length=329, Percent_Identity=23.1003039513678, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081923, Length=399, Percent_Identity=22.0551378446115, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 56669; Mature: 56669

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVPGLRYRDEWLPNFFPAKQVGPDHFVRLSRTGAMQLLSGAENAQLDEISMDEALFERL
CCCCCCCCHHHCCCCCCCHHHCCHHHHEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
EQTSHLITAQNSSRVLEDLKRWHDRTYAGPGLHIVVLSKRCNLNCTYCHMNPENISADSK
HHHHHEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEECCHHCCCCCCH
RFDMQPDVADAIIRFALGSPAPFLGFEFQGGEPFLNFEGMKYFVEEARRQNEAVGKRLRF
HCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SVVSNLMVATDEHIAYCKENRINVSYSLNGPEDIHDSFRVTRSGAGSFRKVMERLSHFRD
HHHHHHHHHCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
RFPGVLSASPLCVVGASNAKELKRTIDFYHAEGFKDLAILKLKALGNARKEKLPFDIQDF
HCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHCCCCCHHHH
LHYYLDALDYIYEKNRQENENYSERVVRVVLAKILQSTDTGFVDWRNPVGDFGSAITYDH
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHCCEEEECC
DGEILPSDEARSMRSEFGLGNVKGTTYDALIRNKATFRSMNLSLRDRDPQCRECAFNPYC
CCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHCCCEECCCCCHHHHCCCCCCC
GVLPVLDYARTGDATPRPHASEECLQTLAVVEWVLRKLVEDPIPLLRMMPGMDAALVEMA
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHH
NGLPTEPSSQAMPPMLSVQSG
CCCCCCCCCCCCCCCEECCCC
>Mature Secondary Structure
MRVPGLRYRDEWLPNFFPAKQVGPDHFVRLSRTGAMQLLSGAENAQLDEISMDEALFERL
CCCCCCCCHHHCCCCCCCHHHCCHHHHEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
EQTSHLITAQNSSRVLEDLKRWHDRTYAGPGLHIVVLSKRCNLNCTYCHMNPENISADSK
HHHHHEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEECCHHCCCCCCH
RFDMQPDVADAIIRFALGSPAPFLGFEFQGGEPFLNFEGMKYFVEEARRQNEAVGKRLRF
HCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SVVSNLMVATDEHIAYCKENRINVSYSLNGPEDIHDSFRVTRSGAGSFRKVMERLSHFRD
HHHHHHHHHCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
RFPGVLSASPLCVVGASNAKELKRTIDFYHAEGFKDLAILKLKALGNARKEKLPFDIQDF
HCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHCCCCCHHHH
LHYYLDALDYIYEKNRQENENYSERVVRVVLAKILQSTDTGFVDWRNPVGDFGSAITYDH
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHCCEEEECC
DGEILPSDEARSMRSEFGLGNVKGTTYDALIRNKATFRSMNLSLRDRDPQCRECAFNPYC
CCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHCCCEECCCCCHHHHCCCCCCC
GVLPVLDYARTGDATPRPHASEECLQTLAVVEWVLRKLVEDPIPLLRMMPGMDAALVEMA
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHH
NGLPTEPSSQAMPPMLSVQSG
CCCCCCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA