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The map label for this gene is gacS [H]

Identifier: 108759989

GI number: 108759989

Start: 279273

End: 280814

Strand: Reverse

Name: gacS [H]

Synonym: MXAN_0230

Alternate gene names: 108759989

Gene position: 280814-279273 (Counterclockwise)

Preceding gene: 108761928

Following gene: 108759725

Centisome position: 3.07

GC content: 70.36

Gene sequence:

>1542_bases
ATGAGGCTTCCCGCCTCCGCCGAGCCTCAGTTCTTCGACGACCTCAGCCGCGAGGTCGCCCTGGCCTGCGACGCGGGCGA
CACCCTCACCTGGGTGGACGCCCGTGCCCGGAACGTCCTCGCCGCCAATCCCGGACAGACGCTGCGCGGACTGGCGGCCC
AGGGCACCGAGGAGAAGGTGGACCGGCTGCTGGTCCAGGCGCACGACGAGAAGGTGGAAGGCTGGGAGCTCATCCTCTGC
CGGGACCAGGTGCCCACGACGTTCGCCTTCCGCGCCCGGCCCCATGACGGAGGCGTGCTGCTGGTGGGCAGCCTGGTGCC
GGAGGACTACGGCGGCGCGCTCGAGCGGGTGAGCACCACCCTGAGCGAGATGTCCGCGCTCCACCGCGAGACGGAGCGCC
AGCAGCGCGAGCTCAAGCGCCGCGCGGACGAACTGGCCCGCCTCAACAACGAGCTGGAGGAATCCAACCGCGGCGTGCGC
AGCCTCCACGCCGCGCTGGACACGGGCATTGGCATCGCGCCGGAGCACCACGAGCGCATCTTCGAGGAGTTCTCGCAGGT
GGAGAGCCACCTGCAACGCAAGGCGAAGGGCACCGGCCTGGGCCTGCCGCTGGCGCGCCGGCTGACGGAGCTCCTGGGCG
GCACGCTCACCGTGAAGAGCGCGCTGGGACAGGGCGCCACGTTCACCGTCACCCTCCCGCGCGTCCACATGGAGGTCACG
GAGATGACCGGCCTGACGGAGCGCAGCGAGCACCTGGACCCCAGCCGGGCCCCGGTGCTGGTGCTGGAGGACGACCGGCA
GACGCTCTTCCTCTACGAGAAGTACCTGGCGCGCTCCGGCTTCCAGGTGCTGCCGGTGCGCAGCACGGATGAGGCCCGGC
GCGTGCTGGAGCGCGTGCGCCCCGCCGCCATGGTGCTGGACGTCATGCTGGAGGGCGAGACGAGCTGGGGCTTCCTGGCG
GACATGAAGAACAACGAGGCCACCCGCGACATCCCCATCCTGGTGGTGACGGTGACGGACCGCGAGCAGAAGGCCCGCGC
GCTGGGCGCCGACGAGTTCTGGCTCAAGCCGGTGGACGAGGTGCGGCTGCAACGCAAGCTGCAGGCCATGGCCCGCACCG
GTCCGGTGGAGCGCATCCTCATCATCGACGACGATGATGTGCACCGCTACCTGCTCACGCAGCTCCTCAAGGACATGCCC
TACGTCCTGCTGGAGGCGGCTGGCGGCAAGGAAGGCGTCCAGTTGGCGCGGGAGAAGTCCCCGCACCTCATCTTCCTGGA
CTTCGTGCTGCCGGACATCACCGCCTTCGACGTGCTGGACGAGCTGAAGGCGGACCCGCGCACGCGTGACGTCCCCGTCA
TCCTCCACACCTCGCACGAGCTGAAGGAGGCCGAGCGCTCGCGGCTGGCGAAGGAGACGGCCGCCATCCTCGCCAAGCAC
ACGCTGAGCCGCGAGGTGGCCATCACCCGCATCCGGGACGCACTGTCCAAGGCCGGCCTGGGCTCCCAAGCGTTGCCGCA
GGAGGCAAGCCGCCGTGGGTGA

Upstream 100 bases:

>100_bases
CCAGTGTGCTGGGCATGTTGAAGTCCGTGCTGGGCTCACGTCACCCCGAAGCACGGACGCTCCTCTCCGAAGCGGGGGTG
TCCTGGGAGGAGACTCGTTC

Downstream 100 bases:

>100_bases
GCCCCATCCAGCCACCGTCCTCAACGTCAACGACGACGACGCCAACCGATACCTCGTCAACCGGCTGCTCTCGCTGGCGG
GATACAACGTGGTGGAGGCG

Product: putative sensor histidine kinase/response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MRLPASAEPQFFDDLSREVALACDAGDTLTWVDARARNVLAANPGQTLRGLAAQGTEEKVDRLLVQAHDEKVEGWELILC
RDQVPTTFAFRARPHDGGVLLVGSLVPEDYGGALERVSTTLSEMSALHRETERQQRELKRRADELARLNNELEESNRGVR
SLHAALDTGIGIAPEHHERIFEEFSQVESHLQRKAKGTGLGLPLARRLTELLGGTLTVKSALGQGATFTVTLPRVHMEVT
EMTGLTERSEHLDPSRAPVLVLEDDRQTLFLYEKYLARSGFQVLPVRSTDEARRVLERVRPAAMVLDVMLEGETSWGFLA
DMKNNEATRDIPILVVTVTDREQKARALGADEFWLKPVDEVRLQRKLQAMARTGPVERILIIDDDDVHRYLLTQLLKDMP
YVLLEAAGGKEGVQLAREKSPHLIFLDFVLPDITAFDVLDELKADPRTRDVPVILHTSHELKEAERSRLAKETAAILAKH
TLSREVAITRIRDALSKAGLGSQALPQEASRRG

Sequences:

>Translated_513_residues
MRLPASAEPQFFDDLSREVALACDAGDTLTWVDARARNVLAANPGQTLRGLAAQGTEEKVDRLLVQAHDEKVEGWELILC
RDQVPTTFAFRARPHDGGVLLVGSLVPEDYGGALERVSTTLSEMSALHRETERQQRELKRRADELARLNNELEESNRGVR
SLHAALDTGIGIAPEHHERIFEEFSQVESHLQRKAKGTGLGLPLARRLTELLGGTLTVKSALGQGATFTVTLPRVHMEVT
EMTGLTERSEHLDPSRAPVLVLEDDRQTLFLYEKYLARSGFQVLPVRSTDEARRVLERVRPAAMVLDVMLEGETSWGFLA
DMKNNEATRDIPILVVTVTDREQKARALGADEFWLKPVDEVRLQRKLQAMARTGPVERILIIDDDDVHRYLLTQLLKDMP
YVLLEAAGGKEGVQLAREKSPHLIFLDFVLPDITAFDVLDELKADPRTRDVPVILHTSHELKEAERSRLAKETAAILAKH
TLSREVAITRIRDALSKAGLGSQALPQEASRRG
>Mature_513_residues
MRLPASAEPQFFDDLSREVALACDAGDTLTWVDARARNVLAANPGQTLRGLAAQGTEEKVDRLLVQAHDEKVEGWELILC
RDQVPTTFAFRARPHDGGVLLVGSLVPEDYGGALERVSTTLSEMSALHRETERQQRELKRRADELARLNNELEESNRGVR
SLHAALDTGIGIAPEHHERIFEEFSQVESHLQRKAKGTGLGLPLARRLTELLGGTLTVKSALGQGATFTVTLPRVHMEVT
EMTGLTERSEHLDPSRAPVLVLEDDRQTLFLYEKYLARSGFQVLPVRSTDEARRVLERVRPAAMVLDVMLEGETSWGFLA
DMKNNEATRDIPILVVTVTDREQKARALGADEFWLKPVDEVRLQRKLQAMARTGPVERILIIDDDDVHRYLLTQLLKDMP
YVLLEAAGGKEGVQLAREKSPHLIFLDFVLPDITAFDVLDELKADPRTRDVPVILHTSHELKEAERSRLAKETAAILAKH
TLSREVAITRIRDALSKAGLGSQALPQEASRRG

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI48994928, Length=242, Percent_Identity=29.7520661157025, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 57232; Mature: 57232

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPASAEPQFFDDLSREVALACDAGDTLTWVDARARNVLAANPGQTLRGLAAQGTEEKV
CCCCCCCCCHHHHHHHHHEEEEECCCCEEEEEEHHHCCEEECCCCHHHHHHHCCCCHHHH
DRLLVQAHDEKVEGWELILCRDQVPTTFAFRARPHDGGVLLVGSLVPEDYGGALERVSTT
HHHHHHHCCCCCCCEEEEEEECCCCCEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHH
LSEMSALHRETERQQRELKRRADELARLNNELEESNRGVRSLHAALDTGIGIAPEHHERI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
FEEFSQVESHLQRKAKGTGLGLPLARRLTELLGGTLTVKSALGQGATFTVTLPRVHMEVT
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEHHHHCCCCEEEEEECHHHHHHH
EMTGLTERSEHLDPSRAPVLVLEDDRQTLFLYEKYLARSGFQVLPVRSTDEARRVLERVR
HHHCCCCHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHC
PAAMVLDVMLEGETSWGFLADMKNNEATRDIPILVVTVTDREQKARALGADEFWLKPVDE
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCHHHHHHCCCCHHHCCCHHH
VRLQRKLQAMARTGPVERILIIDDDDVHRYLLTQLLKDMPYVLLEAAGGKEGVQLAREKS
HHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCC
PHLIFLDFVLPDITAFDVLDELKADPRTRDVPVILHTSHELKEAERSRLAKETAAILAKH
CCEEEEEHHCCCHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHH
TLSREVAITRIRDALSKAGLGSQALPQEASRRG
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MRLPASAEPQFFDDLSREVALACDAGDTLTWVDARARNVLAANPGQTLRGLAAQGTEEKV
CCCCCCCCCHHHHHHHHHEEEEECCCCEEEEEEHHHCCEEECCCCHHHHHHHCCCCHHHH
DRLLVQAHDEKVEGWELILCRDQVPTTFAFRARPHDGGVLLVGSLVPEDYGGALERVSTT
HHHHHHHCCCCCCCEEEEEEECCCCCEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHH
LSEMSALHRETERQQRELKRRADELARLNNELEESNRGVRSLHAALDTGIGIAPEHHERI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
FEEFSQVESHLQRKAKGTGLGLPLARRLTELLGGTLTVKSALGQGATFTVTLPRVHMEVT
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEHHHHCCCCEEEEEECHHHHHHH
EMTGLTERSEHLDPSRAPVLVLEDDRQTLFLYEKYLARSGFQVLPVRSTDEARRVLERVR
HHHCCCCHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHC
PAAMVLDVMLEGETSWGFLADMKNNEATRDIPILVVTVTDREQKARALGADEFWLKPVDE
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCHHHHHHCCCCHHHCCCHHH
VRLQRKLQAMARTGPVERILIIDDDDVHRYLLTQLLKDMPYVLLEAAGGKEGVQLAREKS
HHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCC
PHLIFLDFVLPDITAFDVLDELKADPRTRDVPVILHTSHELKEAERSRLAKETAAILAKH
CCEEEEEHHCCCHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHH
TLSREVAITRIRDALSKAGLGSQALPQEASRRG
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]