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The map label for this gene is tgt [H]

Identifier: 108759647

GI number: 108759647

Start: 5881030

End: 5882166

Strand: Reverse

Name: tgt [H]

Synonym: MXAN_4693

Alternate gene names: 108759647

Gene position: 5882166-5881030 (Counterclockwise)

Preceding gene: 108761956

Following gene: 108757243

Centisome position: 64.36

GC content: 69.13

Gene sequence:

>1137_bases
ATGGTCCGCTTCGAGCTCCTTCACGAGGACACGTCGGGAACGAAGGCCCGCCGGGGGCGTCTGCACACGCCGCACGGCCC
CGTGGAGACACCCATCTTCATGCCCGTGGGCACCGTGGGCAGCGTCAAGGGCGTGGGGCCGGACGACCTGCTCACGCTGG
ACGCGCAGATCATCCTCGGCAACACCTACCACCTGATGCTGCGCCCCGGCGAAGCGCTGGTGGGGGAGATGGGCGGCCTG
CACCGCTTCGTGTCGTGGAACCGGCCCATGCTCACCGACAGCGGCGGGTTCCAGGTGTTCAGCCTGTCCGAGAAGCGCAA
AATCACCGAGGAGGGCGCCGCCTTCCAGTCGCACCTGGACGGCTCGCGGCACTTCCTCACGCCCGAGCGCTCCATCGACA
TCCAGGAGACGCTGGGCGCCGACGTCATCATGGCCTTCGACGAGTGCCCGCCCTCCACCGAGAACAGGGCCTACCTGGAG
GCGTCGCTGGCCCGCACCACCCGCTGGCTGCACCGGTGCGTGAAGGCCTGGAGCCGGGAGCGCTCGTCGCTCTTCGGCAT
CGTCCAGGGCGGCCTGCACGAGGATTTGCGCAAGCGCCACGCCGAGGAGGTGTGCGCGGTGGACCTGCCCGGCTACGCGC
TGGGCGGCTACTCCGTGGGCGAGGCTCCGGAGGCCATGCATGACGGCGTGGCCTACTCCGCGCCCCTGCTGCCCCGCGAC
AAGCCGCGCTACCTGATGGGCGTGGGCACGCCCGTGGACCTGGTCACCTGCGTGGAGCACGGGGTGGACATGTTCGACTG
CGTCCTGCCCACCCGGTGCGCGCGCAACGGCCTGCTCTTCACCTCGGAGGGCAAGGTCGTCATCCGGAACGCGGCCTACG
CCAGGGACAACCGGCCGGTGGACCCTGCTTGCTCCTGCTACACCTGCCGCAATTTCAGCAGGGCCTACCTCCGGCACCTC
TTCGCCGCCCAGGAACTGTTGGCCATGCGGCTCAACACGATTCACAACCTCCACTACTTCCTGGGACTGATGGCAGAAGT
CCGGCGCGCGGTGGCAGAGGACCGGTTCGCCACTTTTGCCCGGGAATTCCGTGCCCGTGCCAAGGCGCAGGAGGCCGAAC
GGACCCGCGGTCGCTGA

Upstream 100 bases:

>100_bases
GTTACGGCGACGCCATGCTGGTGTCGGAGTGAACGCCATGGGCGAGCAGCAGGAAGCGAAGCAGTCAGGTGGCAAGGGCG
ATACCCGCGTGGAGCCGGGG

Downstream 100 bases:

>100_bases
CCGGGCGGATGGATTGCCCGGCTTCCCGCGTTCACTTGCTCACATGGGCCCCCCTTGCTAAGAGGGCCCCCTTTCGGATG
GTTTCCTCGAACAAAATCGG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MVRFELLHEDTSGTKARRGRLHTPHGPVETPIFMPVGTVGSVKGVGPDDLLTLDAQIILGNTYHLMLRPGEALVGEMGGL
HRFVSWNRPMLTDSGGFQVFSLSEKRKITEEGAAFQSHLDGSRHFLTPERSIDIQETLGADVIMAFDECPPSTENRAYLE
ASLARTTRWLHRCVKAWSRERSSLFGIVQGGLHEDLRKRHAEEVCAVDLPGYALGGYSVGEAPEAMHDGVAYSAPLLPRD
KPRYLMGVGTPVDLVTCVEHGVDMFDCVLPTRCARNGLLFTSEGKVVIRNAAYARDNRPVDPACSCYTCRNFSRAYLRHL
FAAQELLAMRLNTIHNLHYFLGLMAEVRRAVAEDRFATFAREFRARAKAQEAERTRGR

Sequences:

>Translated_378_residues
MVRFELLHEDTSGTKARRGRLHTPHGPVETPIFMPVGTVGSVKGVGPDDLLTLDAQIILGNTYHLMLRPGEALVGEMGGL
HRFVSWNRPMLTDSGGFQVFSLSEKRKITEEGAAFQSHLDGSRHFLTPERSIDIQETLGADVIMAFDECPPSTENRAYLE
ASLARTTRWLHRCVKAWSRERSSLFGIVQGGLHEDLRKRHAEEVCAVDLPGYALGGYSVGEAPEAMHDGVAYSAPLLPRD
KPRYLMGVGTPVDLVTCVEHGVDMFDCVLPTRCARNGLLFTSEGKVVIRNAAYARDNRPVDPACSCYTCRNFSRAYLRHL
FAAQELLAMRLNTIHNLHYFLGLMAEVRRAVAEDRFATFAREFRARAKAQEAERTRGR
>Mature_378_residues
MVRFELLHEDTSGTKARRGRLHTPHGPVETPIFMPVGTVGSVKGVGPDDLLTLDAQIILGNTYHLMLRPGEALVGEMGGL
HRFVSWNRPMLTDSGGFQVFSLSEKRKITEEGAAFQSHLDGSRHFLTPERSIDIQETLGADVIMAFDECPPSTENRAYLE
ASLARTTRWLHRCVKAWSRERSSLFGIVQGGLHEDLRKRHAEEVCAVDLPGYALGGYSVGEAPEAMHDGVAYSAPLLPRD
KPRYLMGVGTPVDLVTCVEHGVDMFDCVLPTRCARNGLLFTSEGKVVIRNAAYARDNRPVDPACSCYTCRNFSRAYLRHL
FAAQELLAMRLNTIHNLHYFLGLMAEVRRAVAEDRFATFAREFRARAKAQEAERTRGR

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=362, Percent_Identity=46.1325966850829, Blast_Score=333, Evalue=2e-91,
Organism=Escherichia coli, GI1786607, Length=367, Percent_Identity=50.6811989100817, Blast_Score=379, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17544680, Length=349, Percent_Identity=44.1260744985673, Blast_Score=300, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI25146502, Length=176, Percent_Identity=28.4090909090909, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920510, Length=358, Percent_Identity=45.5307262569832, Blast_Score=322, Evalue=2e-88,
Organism=Drosophila melanogaster, GI21355311, Length=260, Percent_Identity=25, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42155; Mature: 42155

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRFELLHEDTSGTKARRGRLHTPHGPVETPIFMPVGTVGSVKGVGPDDLLTLDAQIILG
CEEEEEEECCCCCCHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEEEEC
NTYHLMLRPGEALVGEMGGLHRFVSWNRPMLTDSGGFQVFSLSEKRKITEEGAAFQSHLD
CEEEEEEECCHHHHHHHCCHHHHHCCCCCEEECCCCEEEEECCHHHHHHHHHHHHHHHCC
GSRHFLTPERSIDIQETLGADVIMAFDECPPSTENRAYLEASLARTTRWLHRCVKAWSRE
CCCEECCCCCCCCHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RSSLFGIVQGGLHEDLRKRHAEEVCAVDLPGYALGGYSVGEAPEAMHDGVAYSAPLLPRD
HHHHHHHHHCCHHHHHHHHHHHHHEEECCCCCEECCCCCCCCCHHHHCCCCCCCCCCCCC
KPRYLMGVGTPVDLVTCVEHGVDMFDCVLPTRCARNGLLFTSEGKVVIRNAAYARDNRPV
CCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCCEEEEECHHHCCCCCC
DPACSCYTCRNFSRAYLRHLFAAQELLAMRLNTIHNLHYFLGLMAEVRRAVAEDRFATFA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REFRARAKAQEAERTRGR
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVRFELLHEDTSGTKARRGRLHTPHGPVETPIFMPVGTVGSVKGVGPDDLLTLDAQIILG
CEEEEEEECCCCCCHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEEEEC
NTYHLMLRPGEALVGEMGGLHRFVSWNRPMLTDSGGFQVFSLSEKRKITEEGAAFQSHLD
CEEEEEEECCHHHHHHHCCHHHHHCCCCCEEECCCCEEEEECCHHHHHHHHHHHHHHHCC
GSRHFLTPERSIDIQETLGADVIMAFDECPPSTENRAYLEASLARTTRWLHRCVKAWSRE
CCCEECCCCCCCCHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RSSLFGIVQGGLHEDLRKRHAEEVCAVDLPGYALGGYSVGEAPEAMHDGVAYSAPLLPRD
HHHHHHHHHCCHHHHHHHHHHHHHEEECCCCCEECCCCCCCCCHHHHCCCCCCCCCCCCC
KPRYLMGVGTPVDLVTCVEHGVDMFDCVLPTRCARNGLLFTSEGKVVIRNAAYARDNRPV
CCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCCEEEEECHHHCCCCCC
DPACSCYTCRNFSRAYLRHLFAAQELLAMRLNTIHNLHYFLGLMAEVRRAVAEDRFATFA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REFRARAKAQEAERTRGR
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA