Click here to switch to the map view.

The map label for this gene is queA [H]

Identifier: 108761956

GI number: 108761956

Start: 5882235

End: 5883350

Strand: Reverse

Name: queA [H]

Synonym: MXAN_4694

Alternate gene names: 108761956

Gene position: 5883350-5882235 (Counterclockwise)

Preceding gene: 108757139

Following gene: 108759647

Centisome position: 64.37

GC content: 73.03

Gene sequence:

>1116_bases
GTGGGGGCTGCTACAAGCGCCACCCCCGTGTCGTCCCGTCTCTCTGATTACGACTTCGCGCTGCCCGAATCCCAGATCGC
CCAGGCTCCCCTGGCCGAGCGGGACGCGTCGCGCTTGATGACCATCAGCCGCTCCAGCGGCACCTGGTCGCACCGCCGGT
TCGCCGACCTCCAGGAGCTCCTGCGCCCCGGAGACCTGCTCGTCCTCAACGACGCCCGCGTCATTCCCGCGCGTCTGCTG
GGCCAGAAGGCCGGCACTGGTGGCCGCGTGGAGCTGCTCGTCGTGCGCCCGGCCGCCCAGAACACGCTGACCTCGGCGGC
GCTCGGGGCGGCCCCCGAGTCGCTCGACTGGGTCTGTCTGGGGCAGGCCTCCAAGGGGCTCAAGCCCGGGCAGCGCGTGA
TGTTCGCCAGCGGCCTGGAGGCCGAAATCCAGGAGGCGCTGGGAGGAGGGGAGTACCGCGTGCGGTTCCACGCACCCCCG
GGCGCCTCGCTGGCCGCGCTGTTGGACGCGGCGGGCCGCCTGCCGCTGCCGCCCTACATCACCCGGGAGCCGGACGCCGC
GGACGCGGAGCGCTACCAGACCGTGTACGCACGCGCCTCCGGAGCCGTGGCCGCGCCCACCGCGGGCCTGCACTTCACCC
AGGAGACCCTGGCCGCCCTGGCTGCGCGTGGCGTGCACCGGGCGCTGGTGACGCTGGACGTGGGGCCAGGCACCTTCCTG
CCGGTGCGTGAGGACGACCTGGACAAGCACCACATGCACCCGGAGCGCTACACCGTCCCGGAGGCCACTGCGAAGGCCAT
CAACGCGGCGCGCGCGGAAGGCCGGCGCGTGGTGGCGGTGGGGACCACCGTGGTGCGCACCCTGGAGTCCGCCACCGACC
CGGAGACGGGGCGGCTGCGCGCGGGCCCCGGCGAGACGACCCTCTTCATCCGTCCCGGGTTCGCCTTCCGTCAGGTGGAC
CTGCTGCTCACCAACTTCCACCTGCCGCGTTCGACGCTGGTGGTGCTGGTGAGCGCGCTGCTCGGACGCGAGCGCACGCT
GGAGGCCTACGCGGAGGCGGTCCGCGCGGGCTACCGATTCTTCAGTTACGGCGACGCCATGCTGGTGTCGGAGTGA

Upstream 100 bases:

>100_bases
GGCCGGGAGTACCGGGAGATCCTCTCCCACTACTACCCCGGGGCCGAGCTGCAGCAGCTCTACTGACGCCTCCCGCGTCC
GGCGCGCTTTCCGCGACGGC

Downstream 100 bases:

>100_bases
ACGCCATGGGCGAGCAGCAGGAAGCGAAGCAGTCAGGTGGCAAGGGCGATACCCGCGTGGAGCCGGGGATGGTCCGCTTC
GAGCTCCTTCACGAGGACAC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MGAATSATPVSSRLSDYDFALPESQIAQAPLAERDASRLMTISRSSGTWSHRRFADLQELLRPGDLLVLNDARVIPARLL
GQKAGTGGRVELLVVRPAAQNTLTSAALGAAPESLDWVCLGQASKGLKPGQRVMFASGLEAEIQEALGGGEYRVRFHAPP
GASLAALLDAAGRLPLPPYITREPDAADAERYQTVYARASGAVAAPTAGLHFTQETLAALAARGVHRALVTLDVGPGTFL
PVREDDLDKHHMHPERYTVPEATAKAINAARAEGRRVVAVGTTVVRTLESATDPETGRLRAGPGETTLFIRPGFAFRQVD
LLLTNFHLPRSTLVVLVSALLGRERTLEAYAEAVRAGYRFFSYGDAMLVSE

Sequences:

>Translated_371_residues
MGAATSATPVSSRLSDYDFALPESQIAQAPLAERDASRLMTISRSSGTWSHRRFADLQELLRPGDLLVLNDARVIPARLL
GQKAGTGGRVELLVVRPAAQNTLTSAALGAAPESLDWVCLGQASKGLKPGQRVMFASGLEAEIQEALGGGEYRVRFHAPP
GASLAALLDAAGRLPLPPYITREPDAADAERYQTVYARASGAVAAPTAGLHFTQETLAALAARGVHRALVTLDVGPGTFL
PVREDDLDKHHMHPERYTVPEATAKAINAARAEGRRVVAVGTTVVRTLESATDPETGRLRAGPGETTLFIRPGFAFRQVD
LLLTNFHLPRSTLVVLVSALLGRERTLEAYAEAVRAGYRFFSYGDAMLVSE
>Mature_370_residues
GAATSATPVSSRLSDYDFALPESQIAQAPLAERDASRLMTISRSSGTWSHRRFADLQELLRPGDLLVLNDARVIPARLLG
QKAGTGGRVELLVVRPAAQNTLTSAALGAAPESLDWVCLGQASKGLKPGQRVMFASGLEAEIQEALGGGEYRVRFHAPPG
ASLAALLDAAGRLPLPPYITREPDAADAERYQTVYARASGAVAAPTAGLHFTQETLAALAARGVHRALVTLDVGPGTFLP
VREDDLDKHHMHPERYTVPEATAKAINAARAEGRRVVAVGTTVVRTLESATDPETGRLRAGPGETTLFIRPGFAFRQVDL
LLTNFHLPRSTLVVLVSALLGRERTLEAYAEAVRAGYRFFSYGDAMLVSE

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=362, Percent_Identity=45.5801104972376, Blast_Score=291, Evalue=4e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39638; Mature: 39506

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAATSATPVSSRLSDYDFALPESQIAQAPLAERDASRLMTISRSSGTWSHRRFADLQEL
CCCCCCCCCHHHHCCCCCCCCCHHHHHHCCCHHHHHHHEEEEECCCCCCHHHHHHHHHHH
LRPGDLLVLNDARVIPARLLGQKAGTGGRVELLVVRPAAQNTLTSAALGAAPESLDWVCL
HCCCCEEEECCCCCCHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCEEEE
GQASKGLKPGQRVMFASGLEAEIQEALGGGEYRVRFHAPPGASLAALLDAAGRLPLPPYI
CCCCCCCCCCCEEEEECCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCC
TREPDAADAERYQTVYARASGAVAAPTAGLHFTQETLAALAARGVHRALVTLDVGPGTFL
CCCCCCCHHHHHHHHHHHCCCCEECCCCCCEEHHHHHHHHHHHCCEEEEEEEECCCCCEE
PVREDDLDKHHMHPERYTVPEATAKAINAARAEGRRVVAVGTTVVRTLESATDPETGRLR
CCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECHHHHHHHHHCCCCCCCCEE
AGPGETTLFIRPGFAFRQVDLLLTNFHLPRSTLVVLVSALLGRERTLEAYAEAVRAGYRF
CCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCE
FSYGDAMLVSE
EECCCEEEECC
>Mature Secondary Structure 
GAATSATPVSSRLSDYDFALPESQIAQAPLAERDASRLMTISRSSGTWSHRRFADLQEL
CCCCCCCCHHHHCCCCCCCCCHHHHHHCCCHHHHHHHEEEEECCCCCCHHHHHHHHHHH
LRPGDLLVLNDARVIPARLLGQKAGTGGRVELLVVRPAAQNTLTSAALGAAPESLDWVCL
HCCCCEEEECCCCCCHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCEEEE
GQASKGLKPGQRVMFASGLEAEIQEALGGGEYRVRFHAPPGASLAALLDAAGRLPLPPYI
CCCCCCCCCCCEEEEECCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCC
TREPDAADAERYQTVYARASGAVAAPTAGLHFTQETLAALAARGVHRALVTLDVGPGTFL
CCCCCCCHHHHHHHHHHHCCCCEECCCCCCEEHHHHHHHHHHHCCEEEEEEEECCCCCEE
PVREDDLDKHHMHPERYTVPEATAKAINAARAEGRRVVAVGTTVVRTLESATDPETGRLR
CCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECHHHHHHHHHCCCCCCCCEE
AGPGETTLFIRPGFAFRQVDLLLTNFHLPRSTLVVLVSALLGRERTLEAYAEAVRAGYRF
CCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCE
FSYGDAMLVSE
EECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA