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The map label for this gene is hyfR [H]

Identifier: 108759402

GI number: 108759402

Start: 3623747

End: 3625096

Strand: Reverse

Name: hyfR [H]

Synonym: MXAN_3095

Alternate gene names: 108759402

Gene position: 3625096-3623747 (Counterclockwise)

Preceding gene: 108761451

Following gene: 108758858

Centisome position: 39.66

GC content: 70.81

Gene sequence:

>1350_bases
ATGGAAGTCGAAGGTGGGCGTGACACGCCGCTGCACGTCACGCTGCCCTACGAGCACGCGCGCCGGGGCTATGACGCCTC
CACGGTGCGCCGGTTCCGGCTCACGGTGGCGGAAGGCCCGAACATGGGGACGACGTGGGAGTCCACGGGCGACACCTGCT
CGGTGGGCTCGCACCCGCTGAACGACTTCGCCGTGGAGGACTCCACGGTGTCCCGGTTCCACTGCGAGGTGCGCATCGGG
CCACGGGGCCCCCAGGTGAAGGACCTGGACAGCCTCAACGGCGTCATCGTCGACGGGGTGCAGGTGGTGGAGGGCGTGCT
GCGCAGTGGCAGCCTGCTGCGCCTGGGCCGCGTGGTGCTGCGCTTCGACTTCAGCGCGGAGAACAACCGCCTGCCGCTGT
CGGAGAACTCGCGCTTCGGCACGCTGGTGGGCGCCTCCGTGGCCATGCGGGGCTGCTTCGCGATGATGGAGCGCGCGGCG
GGCCGCGACGTGACGGTGCTGCTCGAAGGCGAGACGGGCACGGGCAAGAGCCAGGCCGCGCTGGCCATCCACCAGGCCAG
TCCCCGGCGCGACGCGCCCTTCCTCATCGTGGACTGCGGCGCCATCCCCGCGCACCTGTTGGAGAGCGAGCTGTTCGGCC
ACGAGAAGGGCGCGTTCACCGGCGCCGTCCAGCGGCGCGCGGGCGTCTTCGAGGAAGCCGACGGCGGCACCGTCTTCCTG
GACGAGATTGGCGAGCTGCCCGCGGAGTTGCAGCCCAAGCTGCTGCGCGTGCTGGAGAACCGCGAGATTCGCCGCGTGGG
CAGCAACACGTATCAGCCGGTGAACGTGCGCCTCCTCGCCGCCACGCACCGAGACCTTCGCGCGGAGGTCAACGCGGGCC
GGTTCCGCTCCGACCTCTTCTTCCGGCTCGCGGTGCTGCGCTTGCTGCTTCCACCGCTGCGTCAGCGTCCGGAGGACCTG
CCCATGTTGGTGGAAGGCATCCTCGACCTGCTCGGCGCGGACCGCGAGCGCACGGGCGCGCTGCGCACGCAGGACTTCCT
GGCCAGGTTGCGGCACGCGGCGTGGCCCGGCAACGTGCGCGAGCTGCGCAACTACCTGGAGCGCTGCCTTGTCTTCGAGG
AAGCAGTCGAGCTCTCCGAGGAAGAAGCCCACCGCTCCGGCCCACCCGAGGTGGACCCCTCGCAGCCGTACGCCGACCAG
CGCCGTCACGTCGTCGACGACTTCGAGCGCCGCTATCTGCGCGCGCTGCTGGAGAAACACCAGGGCAAGGTGGCCCAGGC
AGCCGTCACCGCGGGCATGGACCGCGTCCACCTCTACCGCCTGCTGCGACGCCACGGCATCAAGCCGTGA

Upstream 100 bases:

>100_bases
GGATGTCGGCTCCCGTGATATCTTGACTTCCATGTCTGCACTGGATCGGGCTCATGCCGCACCCTCCTCGCCGGACGTGA
AGGGCGACTCGCAATGGAAG

Downstream 100 bases:

>100_bases
GCCTCACCGCGGGCGGCTGACGCGCAAGCACCCCAGTCCCGCGGCGGACACGGGTGTCACCAGGAAGGCCGTTTCCAGGC
CCTCGAGCGCCCGAGCGGTC

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MEVEGGRDTPLHVTLPYEHARRGYDASTVRRFRLTVAEGPNMGTTWESTGDTCSVGSHPLNDFAVEDSTVSRFHCEVRIG
PRGPQVKDLDSLNGVIVDGVQVVEGVLRSGSLLRLGRVVLRFDFSAENNRLPLSENSRFGTLVGASVAMRGCFAMMERAA
GRDVTVLLEGETGTGKSQAALAIHQASPRRDAPFLIVDCGAIPAHLLESELFGHEKGAFTGAVQRRAGVFEEADGGTVFL
DEIGELPAELQPKLLRVLENREIRRVGSNTYQPVNVRLLAATHRDLRAEVNAGRFRSDLFFRLAVLRLLLPPLRQRPEDL
PMLVEGILDLLGADRERTGALRTQDFLARLRHAAWPGNVRELRNYLERCLVFEEAVELSEEEAHRSGPPEVDPSQPYADQ
RRHVVDDFERRYLRALLEKHQGKVAQAAVTAGMDRVHLYRLLRRHGIKP

Sequences:

>Translated_449_residues
MEVEGGRDTPLHVTLPYEHARRGYDASTVRRFRLTVAEGPNMGTTWESTGDTCSVGSHPLNDFAVEDSTVSRFHCEVRIG
PRGPQVKDLDSLNGVIVDGVQVVEGVLRSGSLLRLGRVVLRFDFSAENNRLPLSENSRFGTLVGASVAMRGCFAMMERAA
GRDVTVLLEGETGTGKSQAALAIHQASPRRDAPFLIVDCGAIPAHLLESELFGHEKGAFTGAVQRRAGVFEEADGGTVFL
DEIGELPAELQPKLLRVLENREIRRVGSNTYQPVNVRLLAATHRDLRAEVNAGRFRSDLFFRLAVLRLLLPPLRQRPEDL
PMLVEGILDLLGADRERTGALRTQDFLARLRHAAWPGNVRELRNYLERCLVFEEAVELSEEEAHRSGPPEVDPSQPYADQ
RRHVVDDFERRYLRALLEKHQGKVAQAAVTAGMDRVHLYRLLRRHGIKP
>Mature_449_residues
MEVEGGRDTPLHVTLPYEHARRGYDASTVRRFRLTVAEGPNMGTTWESTGDTCSVGSHPLNDFAVEDSTVSRFHCEVRIG
PRGPQVKDLDSLNGVIVDGVQVVEGVLRSGSLLRLGRVVLRFDFSAENNRLPLSENSRFGTLVGASVAMRGCFAMMERAA
GRDVTVLLEGETGTGKSQAALAIHQASPRRDAPFLIVDCGAIPAHLLESELFGHEKGAFTGAVQRRAGVFEEADGGTVFL
DEIGELPAELQPKLLRVLENREIRRVGSNTYQPVNVRLLAATHRDLRAEVNAGRFRSDLFFRLAVLRLLLPPLRQRPEDL
PMLVEGILDLLGADRERTGALRTQDFLARLRHAAWPGNVRELRNYLERCLVFEEAVELSEEEAHRSGPPEVDPSQPYADQ
RRHVVDDFERRYLRALLEKHQGKVAQAAVTAGMDRVHLYRLLRRHGIKP

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=350, Percent_Identity=44.2857142857143, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1788905, Length=288, Percent_Identity=46.5277777777778, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1790437, Length=316, Percent_Identity=44.620253164557, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1788550, Length=329, Percent_Identity=39.5136778115502, Blast_Score=228, Evalue=8e-61,
Organism=Escherichia coli, GI1789087, Length=318, Percent_Identity=42.4528301886792, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI87082152, Length=317, Percent_Identity=42.2712933753943, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1790299, Length=223, Percent_Identity=48.4304932735426, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1786524, Length=359, Percent_Identity=40.1114206128134, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=38.125, Blast_Score=198, Evalue=8e-52,
Organism=Escherichia coli, GI1787583, Length=246, Percent_Identity=41.4634146341463, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI87081872, Length=236, Percent_Identity=44.0677966101695, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1789828, Length=232, Percent_Identity=39.6551724137931, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87081858, Length=292, Percent_Identity=30.1369863013699, Blast_Score=115, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49990; Mature: 49990

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS50006 FHA_DOMAIN ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVEGGRDTPLHVTLPYEHARRGYDASTVRRFRLTVAEGPNMGTTWESTGDTCSVGSHPL
CCCCCCCCCCEEEEECHHHHHCCCCHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
NDFAVEDSTVSRFHCEVRIGPRGPQVKDLDSLNGVIVDGVQVVEGVLRSGSLLRLGRVVL
CCCCCCCCCEEEEEEEEEECCCCCCCCCHHHCCCEEEHHHHHHHHHHCCCCHHHHHHHHE
RFDFSAENNRLPLSENSRFGTLVGASVAMRGCFAMMERAAGRDVTVLLEGETGTGKSQAA
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHE
LAIHQASPRRDAPFLIVDCGAIPAHLLESELFGHEKGAFTGAVQRRAGVFEEADGGTVFL
EEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCEEEH
DEIGELPAELQPKLLRVLENREIRRVGSNTYQPVNVRLLAATHRDLRAEVNAGRFRSDLF
HHHCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEEEEHHHHHHHHCCCCHHHHHHH
FRLAVLRLLLPPLRQRPEDLPMLVEGILDLLGADRERTGALRTQDFLARLRHAAWPGNVR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHCCCCCCHH
ELRNYLERCLVFEEAVELSEEEAHRSGPPEVDPSQPYADQRRHVVDDFERRYLRALLEKH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QGKVAQAAVTAGMDRVHLYRLLRRHGIKP
CCHHHHHHHHHCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEVEGGRDTPLHVTLPYEHARRGYDASTVRRFRLTVAEGPNMGTTWESTGDTCSVGSHPL
CCCCCCCCCCEEEEECHHHHHCCCCHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
NDFAVEDSTVSRFHCEVRIGPRGPQVKDLDSLNGVIVDGVQVVEGVLRSGSLLRLGRVVL
CCCCCCCCCEEEEEEEEEECCCCCCCCCHHHCCCEEEHHHHHHHHHHCCCCHHHHHHHHE
RFDFSAENNRLPLSENSRFGTLVGASVAMRGCFAMMERAAGRDVTVLLEGETGTGKSQAA
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHE
LAIHQASPRRDAPFLIVDCGAIPAHLLESELFGHEKGAFTGAVQRRAGVFEEADGGTVFL
EEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCEEEH
DEIGELPAELQPKLLRVLENREIRRVGSNTYQPVNVRLLAATHRDLRAEVNAGRFRSDLF
HHHCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEEEEHHHHHHHHCCCCHHHHHHH
FRLAVLRLLLPPLRQRPEDLPMLVEGILDLLGADRERTGALRTQDFLARLRHAAWPGNVR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHCCCCCCHH
ELRNYLERCLVFEEAVELSEEEAHRSGPPEVDPSQPYADQRRHVVDDFERRYLRALLEKH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QGKVAQAAVTAGMDRVHLYRLLRRHGIKP
CCHHHHHHHHHCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]